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Anhai Chen,Chufeng He,Yong Feng,Jie Ling,Xin Peng,Xianlin Liu,Shuang Mao,Yongjia Chen,Mengyao Qin,Shuai Zhang,Yijiang Bai,Jian Song,Zhili Feng,Lu Ma,Dinghua He,Lingyun Mei1 대한이비인후과학회 2023 Clinical and Experimental Otorhinolaryngology Vol.16 No.4
Objectives. Branchio-oto syndrome (BOS) primarily manifests as hearing loss, preauricular pits, and branchial defects. EYA1is the most common pathogenic gene, and splicing mutations account for a substantial proportion of cases. However,few studies have addressed the structural changes in the protein caused by splicing mutations and potential pathogenicfactors, and several studies have shown that middle-ear surgery has limited effectiveness in improving hearing in thesepatients. BOS has also been relatively infrequently reported in the Chinese population. This study explored the ge-netic etiology in the family of a proband with BOS and provided clinical treatment to improve the patient’s hearing. Methods. We collected detailed clinical features and peripheral blood samples from the patients and unaffected individualswithin the family. Pathogenic mutations were identified by whole-exome sequencing and cosegregation analysis andclassified according to the American College of Medical Genetics and Genomics guidelines. Alternative splicing wasverified through a minigene assay. The predicted three-dimensional protein structure and biochemical experimentswere used to investigate the pathogenicity of the mutation. The proband underwent middle-ear surgery and was fol-lowed up at 1 month and 6 months postoperatively to monitor auditory improvement. Results. A novel heterozygous EYA1 splicing variant (c.1050+4 A >C) was identified and classified as pathogenic (PVS1(RNA),PM2, PP1). Skipping of exon 11 of the EYA1 pre-mRNA was confirmed using a minigene assay. This mutation mayimpair EYA1-SIX1 interactions, as shown by an immunoprecipitation assay. The EYA1-Mut protein exhibited cellularmislocalization and decreased protein expression in cytological experiments. Middle-ear surgery significantly improvedhearing loss caused by bone-conduction abnormalities in the proband. Conclusion. We reported a novel splicing variant of EYA1 in a Chinese family with BOS and revealed the potential molec-ular pathogenic mechanism. The significant hearing improvement observed in the proband after middle-ear surgeryprovides a reference for auditory rehabilitation in similar patients.
Mei-yu Lv,Kai-yu Liu,Yan Li,Lai Wei,Jian-jian Zhong,Geng Su 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.5
A three-dimensional (3D) Co3O4/mildly oxidized multiwalled carbon nanotubes (moCNTs)/reduced mildly oxidized graphene oxide (rmGO) ternary composite was prepared via a simple and green hydrolysishydrothermal approach by mixing Co(Ac)2·4H2O with moCNTs and mGO suspension in mixed ethanol/H2O. As characterized by scanning electron microscopy and transmission electron microscopy, Co3O4 nanoparticles with size of 20-100 nm and moCNTs are effectively anchored in mGO. Cyclic voltammetry and galvanostatic charge-discharge measurements were adopted to investigate the electrochemical properties of Co3O4/moCNTs/ rmGO ternary composite in 6 M KOH solution. In a potential window of 0-0.6 V vs. Hg/HgO, the composite delivers an initial specific capacitance of 492 F g−1 at 0.5 A g−1 and the capacitance remains 592 F g−1 after 2000 cycles, while the pure Co3O4 shows obviously capacitance fading, indicating that rmGO and moCNTs greatly enhance the electrochemical performance of Co3O4.
Jian Mei,Zan Qu,Songjian Zhao,Xiaofang Hu,Haomiao Xu,Naiqiang Yan 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Co3O4 catalysts modified with Mn and Ti, prepared by co-precipitation method, were used for catalytic oxidation of dibromomethane (CH2Br2), a model molecule for brominated volatile organic compounds (BVOCs). Addition of Ti or Ti + Mn distorted the crystal structure and led to the formation of a Co–O–Ti solid solution. The addition of Mn further enhanced the surface acidity and redox ability of the catalysts. Co–Mn–Ti exhibited the highest activity with a T90 of approximately 234 °C and the highest selectivity to CO2 at low temperatures. Additionally, Co–Mn–Ti showed good stability for at least 30 h at 500 ppm CH2Br2, 0 or 2 vol% H2O, 0 or 500 ppm p-xylene (PX), and 10% O2 at a gas hourly space velocity of 60,000 h−1, and the final products were COx, Br2, and HBr, without the formation of other Br-containing organic byproducts. This high catalytic activity was attributed to its high specific area, high surface acidity, and strong redox property. Furthermore, the synergetic effect of Co, Mn, and Ti made it superior for CH2Br2 oxidation. A plausible reaction mechanism for CH2Br2 oxidation over Co–Mn–Ti catalysts was proposed based on the analysis of the products and in situ diffuse-reflectance infrared Fourier transform spectroscopy results.
Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
( Jian Mei Lu ),( Xiao Hong Zhang ),( Nan Zhang ),( Qing Zhong Kong ),( Yun Feng Zhao ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.2
Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the fl rst time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet signifl cantly decreased the viability of A549 cells at 5-15 M. Tetrazolium violet -induced apoptosis in A549 cells was confl rmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These fl ndings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.
Design of Co3O4/CeO2–Co3O4 hierarchical binary oxides for the catalytic oxidation of dibromomethane
Jian Mei,Jiangkun Xie,Yaning Sun,Zan Qu,Naiqiang Yan 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.73 No.-
Developing the catalysts that can efficiently degrade brominated volatile organic compounds (Br-VOCs)is a priority due to the low activity, the low product selectivity, and the low resistance to Br-poisoning. Inthis work, Co3O4/CeO2–Co3O4 hierarchical binary oxides were designed as a high-performance catalystfor the catalytic oxidation of dibromomethane (CH2Br2) as a model pollutant for Br-VOCs. The surface ofcarbon spheres was coated with a layer of CeO2 nanoparticles, and core-shell CSs@CeO2 were theprecursors of Co3O4/CeO2–Co3O4 hierarchical binary oxides. Co3O4/CeO2–Co3O4(HS) had a hierarchicalporous structure, there was a strong interaction between Co3O4 and CeO2 Co3O4/CeO2–Co3O4(HS)showed an excellent catalytic activity, and its T90 value was only 321 C. Meanwhile, Co3O4/CeO2–Co3O4(HS) showed good stability for at least 30 h. Co3O4 provided the active sites for CH2Br2 oxidation,and the hierarchical porous structure and high specific surface area were conducive to the adsorption ofCH2Br2 molecules. Meanwhile, CeO2 promoted the oxygen mobility of the composite and the oxidationperformance of Co3O4. CH2Br2 molecularfirstly adsorbed on Co3O4/CeO2–Co3O4(HS) surface, anddissociated to form the intermediates (i.e., formate species, and methoxy species), which werefinallyoxidized to CO and CO2, and Br species were removed in the form of HBr and Br2.
Jian Mei,Pengxiang Sun,Xin Xiao,Qi Zhang,Hui Zhao,Yongfu Guo,Shijian Yang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.75 No.-
Optimizing the performance of commercial SCR catalyst (i.e., V2O5–WO3/TiO2) for Hg0 oxidationremained stagnant as the influence mechanism of the compositions offlue gas on Hg0 oxidation wasunclear. In this work, the mechanism of Hg0 oxidation and the influence mechanism of the compositionsofflue gas on Hg0 oxidation over V2O5–WO3/TiO2 were investigated. The reaction orders of Hg0 oxidationover V2O5–WO3/TiO2 in regard to both the concentrations of Hg0 and HCl in gas phase wereapproximately 0. Hence, Hg0 oxidation over V2O5–WO3/TiO2 primarily followed the Langmuir–Hinshelwood mechanism, and the elementary reactions of Hg0 oxidation primarily involved the physicaladsorption of Hg0, the formation of Cl* radial, and the reaction of physically adsorbed Hg0 and Cl* radial. SO2, NO, H2O, and NH3 not only restrained the Cl* radial formation but also disturbed the reaction ofphysically adsorbed Hg0 and Cl* radial. Meanwhile, the physical adsorption of Hg0 was restrained by bothH2O and NH3. Hence, Hg0 oxidation over V2O5–WO3/TiO2 was obviously restrained when SO2, NO, H2O,and NH3 were present influe gas.
DESIGN AND IMPLEMENTATION OF LONG-DISTANCE SERIAL COMMUNICATION BASED ON WEB
SUN Jian-mei,ZHOU Da-yong 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-
With the development of the information industry such as internet and so on, Long-distance controlling on the intelligent equipment becomes the direction of the modem people's lives, Long-distance serial communication is good for researching. The paper discusses the long-distance serial communication Based on Web, it introduces the basic structure and the process of realization of the system, analyzes and realizes the software flow of the serial communication, presents the instance of the Long-distance controlling on the manipulator, the same time, it prospects the development of the long-distance based on the embedded system.