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Disruption of ecdysis in Leptinotarsa decemlineata by knockdown of chitin deacetylase 1
Jian-Jian Wu,Li-Li Mu,Zhong-Chao Chen,Kai-Yun Fu,Wen-Chao Guo,Chao Li,Guo-Qing Li 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2
Chitin deacetylases (CDAs) catalyze N-deacetylation of chitin, a crucial process for chitin modification. In the present paper, LdCDA1 was identified in Leptinotarsa decemlineata. It was copiously expressed in larval foregut, hindgut and epidermis. Just before the molt in the first, second and third larval instars, the mRNA levels of LdCDA1 were high. In the fourth (final)-instar larvae, a peak occurred 4 days after ecdysis. In vivo results revealed that LdCDA1 transcriptionally responded, positively and negatively respectively, to 20-hydroxyecdysone and juvenile hormone titers. Moreover, knockdown of LdCDA1 significantly reduced foliage consumption, lengthened developing period and prevented growth in the final instar larvae. Three distinct lethal phenotypes were noted in the LdCDA1 RNAi larvae. About 30% of the RNAi larvae became moribund and finally died; approximately 50% of deformed pupae died as pharate adults; and around 20% of LdCDA1 depleted pupae finally emerged as abnormal adults and eventually died within 1 week after emergence. Furthermore, chitin content was low and the mRNA levels of five chitin biosynthesis transcripts (LdUAP1, LdUAP2, LdChSAa, LdChSAb and LdChSB) were significantly declined in the LdCDA1 RNAi larvae. In addition, glucose, trehalose and glycogen contents were increased in the LdCDA1 depleted hypomorphs, along with highly expressed genes coding for trehalose and glycogen synthesis enzymes. The findings provide a compelling piece of evidence that CDA1 is critical for chitin deposition in L. decemlineata. Moreover, LdCDA1 may be a potential target for control of the larvae.
Jian-Shian Lin,Ming-Hua Chung,Chen-Ming Chen,Fuh-Shyang Juang,Yu-Sheng Tsai,Shu-Wei Chang,Shun-Hsi Wang,Teh-Chao Liao,Lung-Chang Liu 한국물리학회 2010 Current Applied Physics Vol.10 No.5
UV-curable organic/inorganic hybrid composites with gas barrier and heat-dissipating capability have been successfully fast synthesized with microwave irradiation and utilized for the encapsulation of top-emitting organic light-emitting diodes (TEOLEDs). Experimental results manifest that lab-made organic/inorganic hybrid composites can effectively not only obstruct the invasion of moisture as well as oxygen in the atmosphere into the device but also lower the temperature of device. Therefore, the lifetimes of TEOLEDs with their encapsulation are 2.2 folds longer than those without encapsulation.
( Chao Wu ),( Zhong Jian Guo ),( Ke Ping Chen ),( Hong Xing Shen ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.16 No.2
Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.
Investigation of Porous Structure of Aerogel Prepared from Nanofibrillated Cellulose
Chen Gong,Yu Shi,Jian-Ping Ni,Xiao-Bo Yang,Yan-Zhao Liu,Chao Tian 한국펄프·종이공학회 2016 펄프.종이기술 Vol.48 No.6
Cellulose aerogels were prepared through changing the concentration of TEMPO-oxidized cellulose suspension and applying high-pressure homogenization, followed by regular or rapid freeze drying methods. The prepared cellulose aerogel was studied by scanning electron microscopy, BET analysis (nitrogen gas adsorption), and mercury intrusion porosimetry to investigate the effect of variables on the porous properties. In the present study, the porous structure of cellulose aerogel was well obtained by varying concentration of NFC and applying rapid freeze drying approach. The specific surface area of prepared cellulose aerogels varied linearly with adjusting concentrations of NFC, and the highest surface area of cellulose aerogel was attained at 2.0% concentration of NFC in this study. Compared to regular freezing in the freezer, rapid freezing in the liquid nitrogen prepared cellulose aerogel has a lower BET value but more uniform structure with relatively narrow pore size distribution.
Improved thermoelectric performance of n-type Mg3Sb2–Mg3Bi2 alloy with Co element doping
Chen Yanqun,Wang Chao,Ma Zheng,Li Lanwei,Li Shuyao,Wang Jianli 한국물리학회 2021 Current Applied Physics Vol.21 No.-
n-type Mg3Sb2–Mg3Bi2 alloy shows as a potential new thermoelectric material (TE) and has been widely researched recently. The pure phase n-type Mg3⋅20(Sb0⋅3Bi0.7)1.99Te0.01 were prepared by adjusting Mg content with the Bi impurity phase being effectively suppressed. Then, Co element was doped into the pure phase and the electrical conductivity of samples were improved. With a high power factor of 20.3 μW cm 1K 2 for Mg3⋅185Co0⋅015(Sb0⋅3Bi0.7)1.99Te0.01 at 525 K. Additionally, it was found that the phonon scattering is enhanced due to the larger atomic mass of Co comparing to Mg and the lattice thermal conductivity is reduced. As a result, a high ZT value of ~ 1.03 at 525 K is achieved for the Mg3⋅185Co0⋅015(Sb0⋅3Bi0.7)1.99Te0.01.
Yongjie Chen,Ji Xu,Chao Yang,Hongyu Zhang,Feng Wu,Jian Chen,Kai Li,Hailong Wang,Yu Li,Yinghui Li,Zhongquan Dai 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Long-term spaceflight affects numerous organ systems in the body, including metabolic dysfunction. Recently, ample evidence has demonstrated that the liver is a vulnerable organ during spaceflight. However, the changes in hepatocyte proliferation and cell cycle control under microgravity remain largely unexplored. In the present study, we first confirmed that the serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase, biochemical markers of liver function, were altered in rats under tail suspension (TS) conditions to simulate microgravity, as shown in previous reports. Next, we demonstrated that the cell proliferation activity, determined by Ki67, PCNA and PH3, was significantly decreased at the different TS time points (TS for 14, 28 and 42 days) compared with that in the control group. Consistently, the positive cell cycle regulators Ccna2, Ccnd1, Cdk1, Cdk2 and cyclin D3 were also significantly decreased in the TS groups as shown by quantitative real-time PCR and western blotting analysis. Subsequent analysis revealed that the aberrant hepatocyte proliferation inhibition under simulated microgravity was associated with the upregulation of miR-223 in the liver. We further found that miR-223 inhibited the proliferation of Hepa1-6 cells and identified CDK2 and CUL1 as its direct targets. In addition, the decreased expression of CDK2 and CUL1 was negatively correlated with the level of p27 in vitro and in vivo, which may have been responsible for retarding hepatocyte proliferation. Collectively, these data indicate that upregulation of miR-223 was associated with the inhibition of liver cell growth and reveal the role of miR-223 in rat hepatocyte proliferation disorders and the pathophysiological process under simulated microgravity.
A case study on safety integrity level analysis for shale gas station
Yuan-Jian Yang,Chao Huang,Qiu-Yang Zhong,Hai-Jun Chen,Hong-Zhong Huang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.12
Safety integrity level (SIL), as defined in IEC 61511, is a widely used safety performance measure for safety instrumented functions. Shale gas station is an important part of shale gas gathering and transportation system. To ensure the safety of its instrument system is of great significance to the safe and stable operation of the whole gathering and transportation system. This paper is motivated to conduct a case study on safety integrity analysis for shale gas station by using the layer of protection analysis method (LOPA). Firstly, the equipment under control (EUC) is identified. It is followed by the analysis of reaction reasons on safety instrumented function (SIF) from safety instrumented system (SIS). Finally, according to the identification of independent protection layer (IPL) and probability of failure on demand (PFD), the SIL analysis for every SIF is carried out. The result shows that the SIS of this shale gas station has reached the required of SIL and there is no need for further increase in SIS. Utilizing this method can accurately determine the reliability of the SIS in the station, and it can also be the reference of safe operation for this shale gas station.