Research on the Application of BIM in the Construction Industry

Jiajun Huang 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.8

BIM is a major innovation in the past ten years, it has had a broad and far-reaching impact in the construction industry. Based on the research and analysis of the application value and application status of BIM, this paper forecasts the application prospect of BIM, and puts forward the application scope and application mode of BIM in the construction industry. In this paper, the author participate in B IM projects, he details BIM’s Technology of Application in the green building design and analysis process; he explore the using BIM Technology for green building design and simulation analysis, finally ,he summarizes the technical advantages.

One-Pot Electrodeposition of NiS Nanoparticles as an Efficient Electrode for Nonenzymatic H2O2 and Glucose Sensors

Zhiheng Huang,Chunchuan Gu,Jiajun Wen,Langlang Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1

In this paper, a new and one-pot electrodeposition method was expanded for the preparation of NiS nanoparticles-based electrochemical biosensor using metal-ion complexes as a precursor. Thioacetamide was used to control the production rate of NiS nanoparticles for the first time. The proposed electrochemical sensor was characterized by energy dispersive X-ray spectroscopy (EDX), field emission scanning electron microscope (FESEM), cyclic voltammograms (CV), and electrochemical impedance spectra (EIS). Experiment parameters were optimized. Under the optimized condition, the prepared NiS-based biosensor exhibited excellent electrocatalytic oxidation of H2O2 and glucose due to their small size. It provided fast and sensitive strategy for detecting H2O2 and glucose in the range of 1–5000 and 1–1000 μM. The detection limit of 0.257 and 0.3 μM was obtained for H2O2 and glucose. The mechanisms were also analyzed. The proposed biosensor exhibited excellent anti-interference and repeatability. Furthermore, it was applied in the actual sample analysis, such as human blood serum.

Friction and Wear Performance of Double-bump Design of Piston Skirt Main Thrust Side

Bifeng Yin,Jiajun Zu,Bo Xu,Gongyin Huang,Manying Yang 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.6

As a key component of the engine, piston have to work in harsh environment for a very long time, its friction performance and operational reliability have been the research focus. In this paper, a four-cylinder diesel engine was used as the research object. The dynamic model considering multi-factors such as inertial parameters, surface roughness, thermal deformation and elastic deformation was established in Ricardo-Pisdyn software. The research mainly focused on the doublebump design of the main thrust side of the piston (exploring the parameters of the upper bump height, cavity depth etc.) on the friction and wear performance. The research showed that appropriately increasing the depth of the cavity can improve the wear within a certain range. However, if the cavity depth increase too much, the abrasion of the main thrust surface will be aggravated. After optimizing of the clearance and adopting the double-bump profile on the main thrust side, the accumulated wear load of the piston can be significantly reduced and its friction and wear performance will be improved. In this paper, the optimized accumulated wear load decreases from 31.65 MW/m2 to 14.64 MW/m2, drops by 53.7 %.

Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.

Pim-1 Protects Retinal Ganglion Cells by Enhancing Their Regenerative Ability Following Optic Nerve Crush

Shoumei Zhang,Li Shuai,Dong Wang,Tingting Huang,Shengsheng Yang,Mingyong Miao,Fang Liu,Jiajun Xu 한국뇌신경과학회 2020 Experimental Neurobiology Vol.29 No.3

Provirus integration site Moloney murine leukemia virus (Pim-1) is a proto-oncogene reported to be associated with cell proliferation, differentiation and survival. This study was to explore the neuroprotective role of Pim-1 in a rat model subjected to optic nerve crush (ONC), and discuss its related molecules in improving the intrinsic regeneration ability of retinal ganglion cells (RGCs). Immunofluorescence staining showed that AAV2- Pim-1 infected 71% RGCs and some amacrine cells in the retina. Real-time PCR and Western blotting showed that retina infection with AAV2- Pim-1 up-regulated the Pim-1 mRNA and protein expressions compared with AAV2-GFP group. Hematoxylin-Eosin (HE) staining, γ-synuclein immunohistochemistry, Cholera toxin B (CTB) tracing and TUNEL showed that RGCs transduction with AAV2-Pim-1 prior to ONC promoted the survival of damaged RGCs and decreased cell apoptosis. RITC anterograde labeling showed that Pim-1 overexpression increased axon regeneration and promoted the recovery of visual function by pupillary light reflex and flash visual evoked potential. Western blotting showed that Pim- 1 overexpression up-regulated the expression of Stat3, p-Stat3, Akt1, p-Akt1, Akt2 and p-Akt2, as well as βIII-tubulin, GAP-43 and 4E-BP1, and downregulated the expression of SOCS1 and SOCS3, Cleaved caspase 3, Bad and Bax. These results demonstrate that Pim-1 exerted a neuroprotective effect by promoting nerve regeneration and functional recovery of RGCs. In addition, it enhanced the intrinsic regeneration capacity of RGCs after ONC by activating Stat3, Akt1 and Akt2 pathways, and inhibiting the mitochondrial apoptosis pathways. These findings suggest that Pim-1 may prove to be a potential therapeutic target for the clinical treatment of optic nerve injury

Rhaponticin suppresses the hypoxia-induced factor-1 alpha-mediated aggressive phenotype of tongue squamous cell carcinoma

Wu Yuan,Wan Xiaowen,Shao Yisen,Wang Wei,Huang Wenquan,Zhu Jiajun,Jiang Lin 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive.We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia.The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α.Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α. Background Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive. Objective We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia. Results The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α. Conclusions Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α.