Molecular Cloning, Identification and Characteristics of a Novel Isoform of Carbamyl Phosphate Synthetase 1 in Human Testis

( Ran Huo ),( Hui Zhu ),( Li Lu ),( Lan Lan Ying ),( Min Xu ),( Zhi Yang Xu ),( Jian Min Li ),( Zuo Min Zhou ),( Jia Hao Sha ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1

A gene coding a novel isoform of carbamyl phosphate synthetase I (CPSI) was cloned from a human testicular library. As shown by cDNA microarray hybridization, this gene was expressed at a higher level in human adult testes than in fetal testes. The full length of its cDNA was 3831 bp, with a 3149 bp open reading frame, encoding a 1050-amino-acid protein. The cDNA sequence was deposited in the GenBank (AY317138). Sequence analysis showed that it was homologous to the human CPS1 gene. The putative protein contained functional domains composing the intact large subunit of carbamoyl phosphate synthetase, thus indicated it has the capability of arginine biosynthesis. A multiple tissue expression profile showed high expression of this gene in human testis, suggesting the novel alternative splicing form of CPS1 may be correlated with human spermatogenesis.

Hole-filling Based on Disparity Map and Inpainting for Depth-Image-Based Rendering

Ran Liu,Zekun Deng,Lin Yi,Zhenwei Huang,Donghua Cao,Miao Xu,Ruishuang Jia 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.5

Due to the changes in viewpoint, holes may appear in the novel view synthesized by depth-image-based rendering (DIBR). A hole-filling method combining disparity-map-based hole-filling and inpainting is proposed. The method first eliminates matching errors in destination image according to the disparity map. Then, holes are classified into different types according to their sizes. Finally, a disparity-map-based approach and an improved exemplar-based inpainting algorithm are used to fill different types of holes according to the type of hole. Experimental results show that the artifacts at the edge of foreground objects can be reduced in synthesized view since the matching errors are eliminated before hole-filling. In addition, the proposed method can achieve more natural and satisfactory results in filled areas in comparison with the disparity-map-based approach and Gautier’s inpainting algorithm, though it may result in higher time complexity.

양귀비에 관한 드라마의상 분석

가빙(Jia Bing),장애란(Ae Ran Jang) 한국생활과학회 2012 한국생활과학회 학술대회 Vol.2012 No.11

An Improved Method of Maximum Power Point Tracking Strategy for Wind Power Conversion System

CHEN Ran,CHEN Jie,CHEN Jia-wei,CHEN Zhi-hui,GONG Chun-ying,YAN Yang-guang 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

The turbine speed loop adjustment is the key link of the designing of the controller for the fixed pitch variable speed wind energy conversion systems (WECS). Because of the strong nonlinear, big inertia and mechanical damping characteristics, it’s difficult to designing the controller of the wind power systems. In this paper, a small signal model is present, and based on the analyzing; a wind turbine speed loop regulator is designed. Then, a method of tracking the peak power conversion system is proposed, which is independent of the turbine parameters and air density. At last, simulation system is built, and the results of the simulation experiments show that, the performance of the controller algorithm meet the requirements of the MPPT without wind measurement.

Core fucosylation of maternal milk N-glycan prevents intestinal inflammation by regulating Treg/Th17 balance in infant gut microbiota

Tao Jia,Ran Yan,Pan Zhongmin,Wang Li 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1

Background This study elucidated the regulatory roles of milk glycoprotein core fucosylation on the gut microbiota of breast-fed infants. Objectives In order to investigate the effects of core fucosylation of maternal milk N-glycan on the intestinal functions in newborn mice, Fut8 +/+ → Fut8 +/+ and Fut8 ± → Fut8 +/+ models were established. Results The results indicated that the gut microbiota diversity was altered and production of Bifidobacterium and Lactobacillus was reduced in the intestine of Fut8 ± → Fut8 +/+ mice . Furthermore, the levels of SCFAs were decreased in the gut of Fut8 ± → Fut8 +/+ infant. To further investigate the effects of core fucosylation of breast milk N-glycan on the intestinal inflammation in newborns, NEC models were established, and Fut8 +/+ off -springs with experimentally induced NEC were fed with the breast milk of Fut8 +/+ and Fut8 ± maternal mice. Our data revealed that reduced core fucosylation of breast milk N-glycan could be responsible for the intestinal inflammation in NEC off spring, as the production of pro-inflammatory cytokines including IL-1b, IL-6, IL-17A and TNF-a was significantly upregulated in Fut8 ± → Fut8 +/+ NEC group, while the levels of anti-inflammatory cytokine IL-10 were remarkably reduced. Furthermore, Treg/Th17 balance in the intestine was disturbed in Fut8 ± → Fut8 +/+ NEC newborn, suggesting the impaired immune development caused by reduced core fucosylation of breast milk. Conclusion These fi ndings indicated that core fucosylation of maternal milk N-glycan could assist the colonization of gut Lactobacillus and Bifidobacterium and regulate the Treg/Th17 balance, subsequently preventing intestinal inflammation in breast-fed newborns.

Lactic acid bacterial inoculant effects on the vitamin content of alfalfa and Chinese leymus silage

Tingting Jia,Zhiqiang Sun,Run Gao,Zhu Yu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: Information regarding the vitamin content of silage is limited. This study investigated the changes in the vitamin content of alfalfa and Chinese leymus silages with or without a lactic acid bacterial inoculant. Methods: Alfalfa at the early flowering stage and Chinese leymus at the full-bloom stage were harvested. The treatments for each forage type were control (deionized water only) and 1×106 colony-forming units Lactobacillus plantarum (LP)/g fresh matter. After 45 days of ensiling, all silages were sampled for evaluating the vitamin content, fermentation quality and chemical composition. Results: The LP inoculant decreased the pH value and ammonia nitrogen content of the alfalfa and Chinese leymus silages and significantly (p<0.05) increased the lactic acid, acetic acid concentrations and Flieg’s points. Prior to ensiling, the levels of five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) and α-tocopherol in alfalfa were significantly (p<0.01) higher than those in Chinese leymus. Ensiling decreased the levels of the five B-group vitamins in both alfalfa and Chinese leymus while increasing the α-tocopherol content of Chinese leymus. The thiamin, riboflavin, niacin and pantothenic acid levels in the LP-treated silage were significantly (p<0.05) lower than those in the untreated silage for the alfalfa and Chinese leymus. The α-tocopherol content in the LP-treated alfalfa silage was significantly (p<0.05) higher than that in the untreated alfalfa silage. There was no significant (p>0.05) difference in pyridoxine content between the untreated and LP-treated silages for both forages. Conclusion: With or without LP inoculation, the levels of the five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) in alfalfa and Chinese leymus decreased after 45 days of ensiling, while the α-tocopherol content of Chinese leymus increased. The LP inoculant improved the fermentation quality of both the alfalfa and Chinese leymus silages but increased the thiamin, riboflavin, niacin, and pantothenic acid loss in the two forages after fermentation.

User Research에 의한 거실 가구 디자인 방향 연구 - 중국 북경의 맞벌이 가정을 중심으로 -

나가미(Luo, Jia-Wei),안기(An, Qi),최경란(Choi, Kuyng-Ran) 한국실내디자인학회 2022 한국실내디자인학회 학술대회논문집 Vol.24 No.3

Current Status of Opportunistic Infection in Inflammatory Bowel Disease Patients in Asia: A Questionnaire-Based Multicenter Study

Yang Hong,Ran Zhihua,Jin Meng,Qian Jia-Ming 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.5

Background/Aims: Opportunistic infection in inflammatory bowel disease (IBD) has become a serious problem. However, its status of doctors’ opinions and test equipment in hospitals are unclear. The aim of the study was to investigate these issues to improve the prognosis of IBD patients. Methods: This retrospective, multicenter study was conducted by 83 investigators who were members of the Asian Organization for Crohn’s and Colitis. Data on opportunistic infection were collected from hospital databases between January 2017 and December 2017. The survey consisted of 11 items. Results: Most physicians appreciated the diagnostic value of tissue cytomegalovirus (CMV) DNA, accounting for 86.1% of members in China, 37.5% in Japan, 52.9% in South Korea, and 66.7% in Southeast Asia. Only 83.1% of hospitals had the ability to test for CMV immunohistochemistry in Asia. Hepatitis B surface antigen (HBsAg) screening was recommended by all members. However, only 66.7% in China, 70.6% in South Korea, and 66.7% in Southeast Asia agreed to routinely vaccinate IBD patients when HBsAg tested negative. Most members preferred metronidazole (74.7%) as the first choice for patients with Clostridium difficile infection. However, the proportion of stool C. difficile toxin test was lower in China than in other areas (75.0% in China vs 95.8% in Japan and 100% in South Korea and Southeast Asia, p<0.05). Conclusions: Opportunistic infection from CMV, hepatitis B virus, and C. difficile should be of high concern for IBD patients. More efforts are needed, such as understanding consensus in clinical practice and improving testing facilities in hospitals.

Resistance level of Schoenoplectus juncoides (Roxb.) with Pro<SUB>197</SUB> and Trp<SUB>574</SUB> mutations to ALS-inhibitors

Wei Qiang Jia,Ok Jae Won,Cho Kwang Min,Jin-Won Kim,Jeong ran Lee,In Yong Lee,Kee Woong Park 한국잡초학회 2019 한국잡초학회 별책(학술대회 초록집) Vol.39 No.2

메디컬스킨케어센터를 내원한 환자들의 성별에 따른 피부관리 실태, 인지도, 만족도와의 관련성

쟈위에(Yue Jia),김경란(Kyeong-Ran Kim) 한국컴퓨터정보학회 2021 韓國컴퓨터情報學會論文誌 Vol.26 No.6

본 연구에서는 중국 하이난성 지역 10대~50대 남녀를 대상으로 메디컬스킨케어센터를 내원한 환자들을 중심으로 성별에 따른 피부유형 및 피부관리실태, 피부관리 인지도, 만족도를 검증하고자 한다. 이에 피부관리 실태, 피부관리 인지도, 피부관리 만족도 등을 2020년 12월 21일에서 2021년 1월 9일까지 위쳇(WeChat), 왠쥬엔씽 프로그램(wenjuanxing program)을 이용하여 총 328부를 조사하여 SPSSWIN 21.0 프로그램을 사용하여 분석하였다. 피부유형 및 피부관리실태, 피부관리 인지도 및 만족도는 빈도분석(Frequency Analysis)을 실시하였고, 피부관리 인지도와 만족도 신뢰도는 Cronbach‘s α의 계수를 구하였다. 성별에 따른 피부유형 및 피부관리실태, 인지도, 만족도의 관련성은 카이스케어 검정(χ²)과 t-test를 실시하였다. 분석결과 성별에 따른 피부타입은 여성은 건성피부, 남성은 지성피부이고, 피부고민은 여성은 기미색소, 남성은 여드름피부로 성별에 따라 차이가 나타났다. 이러한 문제성피부관리는 남녀모두 홈케어가 높았고, 다음으로 여성은 피부과, 남성은 약국으로 유의미한 차이를 나타내었다. 진행기간은 남녀모두 1∼3년 미만이고, 효과적인 피부 개선 방법으로는 남녀모두 좋은 생활습관, 레이저 순이었다. 병원 선택 시 고려 사항으로는 유명한 체인병원이고, 관리 시 중요 사항은 의사나 피부관리의 전문성을 고려한 것으로 응답하였다. 피부관리 및 치료 인지도는 여성은 외적, 남성은 내적이 높았고, 피부관리 만족도의 차이는 여성은 서비스, 남성은 효과로 나타났으며, 관리만족도는 남성이 여성보다 유의미하게 더 높은 것으로 나타났다. 결론은 중국 메디컬스킨케어센터를 내원한 환자들이 성별에 따라 피부유형 및 피부고민, 피부문제, 피부관리 방법, 피부관리 만족도에 차이가 있는 것으로 분석되어 다양한 제품 개발 및 체계적인 관리프로그램의 필요성을 제시하였다. Chinese people have increasingly high interests in skin care and trust and prefer medical institution products and equipment to treat skin problems. The purpose of this study is to examine skin types and skin care situations, skin care recognition, and skin care satisfaction by gender in medical skin care center patients from in their 10s to 50s in Hainan Province, China. The questionnaire survey consisted of general characteristics(n=8), skin care situations(n=6), skin care recognition(n=11), and skin care satisfaction(n=21). A total of 328 questionnaires were researched from December 21, 2020 to January 9, 2021 using WeChat and Wenjuanxing program. Data were analyzed by SPSSWIN 21.0. Frequency analysis was applied for general characteristics, skin care situations, skin care recognition, and skin care satisfaction and Cronbach’s α was used for the reliability of skin care recognition and satisfaction. The relationship among skin care situations, skin care recognition, and skin care satisfaction was analyzed by χ2 test and t-test. As a result, the common skin types by gender was dry skin in females and oily skin in males. The highest skin trouble was melasma and pigments in females and pimple in males. The most common way to manage troubled skin was homecare in both females and males, followed by the dermatology department in females and pharmacy in males, suggesting a significant difference. The common period of skin trouble was from one year to three years and the most effective way to improve skin was good life habit, followed by laser treatment in both females and males. The most important consideration to choose a hospital was a famous franchise hospital and the most important matters in management was doctor or skin care professionalism. Skin care and treatment recognition was high in external effects for females and internal effects for males. Skin care satisfaction was high in service for females and effect for males. Skin care satisfaction was significantly higher in males than in females. In conclusion, there was a difference in skin types, skin troubles, skin problems, skin care ways, and skin care satisfaction by gender in Chinese medical skin care center patients. Therefore, this study suggests the development of various products and the need of systematic management programs.