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All-Trans Retinoic Acid에 의한 HL-60 세포 분화유도중 H2B Histone, c-Myc 및 DNA Topoisomerase I 발현에 관한 연구
임규,박정동,최병한,이용진,김계영,이명선,장은미,김삼용,권기량,곽상태,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1
Effects of all-trans retinoic acid(retinoic acid) on DNA replication, H2B histone and DNA topoisopmerase I(Topo I) gene expression have been investigated in human promyelocytic leukemia cell line HL-60. DNA synthesis decreased at 24 hours after exposure of retinoic acid in the HL-60 cells. H2B histone mRNA rapidly reduced at 48 hours and Topo I and c-myc mRNA at 24 hours in retinoic acid-exposured HL-60 cells, respectively. The levels of c-myc, H2B histone and Topo I gene expression were reduced in proportion to the concentration of retinoic acid. The H2B histone mRNA in retinoic acid-exposured cells was elevated by cycloheximide treatment, while the level of Topo I mRNA was constant. These results suggest that regulations of H2B histone, c-myc and Topo I gene expression are different from one another, and repression of Topo I gene is closely correlated with c-myc gene during retinoic acid-induced differentiation of HL-60 cells.
( Jeong Hwan Kim ),( Chung Eun Hwang ),( Chang Kwon Lee ),( Jin Hwan Lee ),( Gyoung Min Kim ),( Seong Hoon Jeong ),( Jeong Hee Shin ),( Jong Sang Kim ),( Kye Man Cho ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7
The changes in the β-glucosidase activity, total phenolic contents, isoflavone contents, and antioxidant activities during the fermentation of cheonggukjang by Bacillus amyloliquefaciens MJ1-4 with and without garlic were investigated. The levels of total phenolic and isoflavonemalonylglycoside, -acetylglycoside, and -aglycone contents increased, whereas the 2,2-diphenyl- 1-picrylhydrazyl (DPPH) and 2,2``-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and ferric reducing/antioxidant power (FRAP) assay results increased, but isoflavone-glycoside levels decreased during cheonggukjang fermentation. The levels of total phenolic and total isoflavone contents and the antioxidant activities were higher in cheonggukjang fermented without garlic (CFWOG) than in cheonggukjang fermented with garlic (CFWG) after 24 h of fermentation, but they were lower in CFWOG than in CFWG after 72h of fermentation. In particular, the highest levels of total phenolic, daidzein, glycitein, and genistein were present at concentrations of 15.18 mg/g, 264.4 μg/g, 16.4 μg/g, and 31.1 μg/g after 72h of fermentation in CFWG, showing 82.89% in DPPH radical scavenging activity, 106.32% in ABTS radical scavenging activity, and 1.47 (OD593 nm) in FRAP assay, respectively. From these results, we suggest that the high antioxidant activity of CFWG might be related to the markedly higher levels of total phenolic contents, isoflavone-malonylglycosides, - acetylglycosides, and -aglycones achieved during fermentation.
( Kye-hwan Kim ),( Kyung Nyeo Jeon ),( Min Gyu Kang ),( Jong Hwa Ahn ),( Jin-sin Koh ),( Yongwhi Park ),( Seok-jae Hwang ),( Young-hoon Jeong ),( Choong Hwan Kwak ),( Jin-yong Hwang ),( Jeong Rang Par 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.5
Background/Aims: This study is a head-to-head comparison of predictive values for long-term cardiovascular outcomes between exercise electrocardiography (ex-ECG) and computed tomography coronary angiography (CTCA) in patients with chest pain. Methods: Four hundred and forty-two patients (mean age, 56.1 years; men, 61.3%) who underwent both ex-ECG and CTCA for evaluation of chest pain were included. For ex-ECG parameters, the patients were classified according to negative or positive results, and Duke treadmill score (DTS). Coronary artery calcium score (CACS), presence of plaque, and coronary artery stenosis were evaluated as CTCA parameters. Cardiovascular events for prognostic evaluation were defined as unstable angina, acute myocardial infarction, revascularization, heart failure, and cardiac death. Results: The mean follow-up duration was 2.8 ± 1.1 years. Fifteen patients experienced cardiovascular events. Based on pretest probability, the low- and intermediate-risks of coronary artery disease were 94.6%. Odds ratio of CACS > 40, presence of plaque, coronary stenosis ≥ 50% and DTS ≤ 4 were significant (3.79, p = 0.012; 9.54, p = 0.030; 6.99, p < 0.001; and 4.58, p = 0.008, respectively). In the Cox regression model, coronary stenosis ≥ 50% (hazard ratio, 7.426; 95% confidence interval, 2.685 to 20.525) was only significant. After adding DTS ≤ 4 to coronary stenosis ≥ 50%, the integrated discrimination improvement and net reclassification improvement analyses did not show significant. Conclusions: CTCA was better than ex-ECG in terms of predicting long-term outcomes in low- to intermediate-risk populations. The predictive value of the combination of CTCA and ex-ECG was not superior to that of CTCA alone.
Short-term results of ABO-incompatible living donor kidney transplantation
Jeong Kye Hwang,Sang Il Kim,Bum Soon Choi,Cheol Woo Yang,Yong Soo Kim,In Sung Moon,Ji Il Kim 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.81 No.1
Purpose: ABO incompatible (ABOi) kidney transplantation (KT) has been increasing to compensate for the shortage of organ donors. However, detailed comparative analyses of ABOi KT with ABO compatible (ABOc) KT are still rare. Methods: This retrospective study compared 12 consecutive ABOi KTs to 50 ABOc KTs that employed the same maintenance immunosuppressive agents during the same period. Comparisons of patient survival, graft survival, graft function, and complications were made until post-transplant day 90. Results: Baseline characteristics of the two groups were similar except for the positivity of panel reactive antibody (12% in the ABOc group vs. 42% in the ABOi group; P = 0.029). There were no significant differences in patient survival, graft survival, post-operative renal function, incidence of acute rejection, infections, or medical and surgical complications. However, bleeding complications were more common in the ABOi group (25%) than versus the ABOc group (6%) (P = 0.08). The preoperative and total hospital stay of the ABOi patients was significantly longer than the ABOc patients (P = 0.001). Conclusion: ABOi KT is a viable and safe option for patients whose only donor is blood incompatible, despite the longer preoperative hospital stay for preparation.
Kye-Hwan Kim,Seung Do Lee,Hyo Jin Lee,Hangyul Kim,Hye Ree Kim,Yun Ho Cho,Jeong Yoon Jang,Min Gyu Kang,Jin-Sin Koh,Seok-Jae Hwang,Jin-Yong Hwang,Jeong Rang Park 한국심초음파학회 2023 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.31 No.2
BACKGROUND: The prognostic utility of follow-up transthoracic echocardiography (FU-TTE) in patients with hypertrophic cardiomyopathy (HCM) is unclear, specifically in terms of whether changes in echocardiographic parameters in routine FU-TTE parameters are associated with cardiovascular outcomes. METHODS: From 2010 to 2017, 162 patients with HCM were retrospectively enrolled in this study. Using echocardiography, HCM was diagnosed based on morphological criteria. Patients with other diseases that cause cardiac hypertrophy were excluded. TTE parameters at baseline and FU were analyzed. FU-TTE was designated as the last recorded value in patients who did not develop any cardiovascular event or the latest exam before event development. Clinical outcomes were acute heart failure, cardiac death, arrhythmia, ischemic stroke, and cardiogenic syncope. RESULTS: Median interval between the baseline TTE and FU-TTE was 3.3 years. Median clinical FU duration was 4.7 years. Septal trans-mitral velocity/mitral annular tissue Doppler velocity (E/e’), tricuspid regurgitation velocity, left ventricular ejection fraction (LVEF), and left atrial volume index (LAVI) at baseline were recorded. LVEF, LAVI, and E/e’ values were associated with poor outcomes. However, no delta values predicted HCM-related cardiovascular outcomes. Logistic regression models incorporating changes in TTE parameters had no significant findings. Baseline LAVI was the best predictor of a poor prognosis. In survival analysis, an already enlarged or increased size LAVI was associated with poorer clinical outcomes. CONCLUSIONS: Changes in echocardiographic parameters extracted from TTE did not assist in predicting clinical outcomes. Cross-sectionally evaluated TTE parameters were superior to changes in TTE parameters between baseline and FU at predicting cardiovascular events.
Comparison of import risk analyses to evaluate the potential veterinary hazards of pork
Soo-Jeong Kye(Soo-Jeong Kye),Hui-Young Hwang(Hui-Young Hwang),Seong-Hee Kim(Seong-Hee Kim),Sung-Ik Moon(Sung-Ik Moon),Jin-Hee Hur(Jin-Hee Hur),Ji-Hye Kim(Ji-Hye Kim),Hve-Sook Chang(Hve-Sook Chang),Byo 한국예방수의학회 2022 한국예방수의학회 학술대회자료집 Vol.2022 No.-
Park, Yongwhi,Tantry, Udaya,Koh, Jin-Sin,Ahn, Jong-Hwa,Kang, Min,Kim, Kye,Jang, Jeong,Park, Hyun,Park, Jeong-Rang,Hwang, Seok-Jae,Park, Ki-Soo,Kwak, Choong,Hwang, Jin-Yong,Gurbel, Paul,Jeong, Young-Ho Thieme 2017 Thrombosis and Haemostasis Vol.117 No.5
<B>Summary</B><P>The role of platelet-leukocyte interaction in the infarct myocardium still remains unveiled. We aimed to determine the linkage of platelet activation to post-infarct left ventricular remodelling (LVR) process. REMODELING was a prospective, observational, cohort trial including patients (n = 150) with ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention. Patients were given aspirin plus clopidogrel therapy (600 mg loading and 75 mg daily). Platelet reactivity (PRU: P2Y12 Reaction Units) was assessed with VerifyNow P2Y12 assay on admission. Transthoracic echocardiography was performed on admission and at one-month follow-up. The primary endpoint was the incidence of LVR according to PRU-based quartile distribution. LVR was defined as a relative ≥ 20 % increase in LV end-diastolic volume (LVEDV) between measurements. Adverse LVR was observed in 36 patients (24.0 %). According to PRU quartile, LVR rate was 10.8 % in the first, 23.1 % in the second, 27.0 % in the third, and 35.1 % in the fourth (p = 0.015): the optimal cut-off of PRU was ≥ 248 (area under curve: 0.643; 95 % confidence interval: 0.543 to 0.744; p = 0.010). LVR rate also increased proportionally according to the level of high sensitivity-C reactive protein (hs-CRP) (p = 0.012). In multivariate analysis, the combination of PRU (≥ 248) and hs-CRP (≥ 1.4 mg/l) significantly increased the predictive value for LVR occurrence by about 21-fold. In conclusion, enhanced levels of platelet activation and inflammation determined the incidence of adverse LVR after STEMI. Combining the measurements of these risk factors increased risk discrimination of LVR. The role of intensified antiplatelet or anti-inflammatory therapy in post-infarct LVR process deserves further study.</P>
Hwang, Seung Hwan,Kim, Kye Hun,Yoon, Hyun Ju,Hong, Young Joon,Kim, Ju Han,Ahn, Young Keun,Jeong, Myung Ho,Cho, Jeong Gwan,Park, Jong Chun,Kang, Jung Chaee Korean Society of Echocardiography 2011 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.19 No.2
<P>The prognosis of stress induced cardiomyopathy (SCMP) is generally known to be excellent, however, several fatal complications such as cardiac rupture and left ventricular (LV) thrombosis with subsequent embolic complications have been described. We report a rare case of SCMP complicated by LV thrombosis and multiple cerebral infarctions in a patient with essential thrombocythemia. After intravenous anticoagulation with heparin and general managements for heart failure and cerebral infarctions, her neurologic symptoms and the wall motion abnormalities of the LV apex were improved, and the thrombus was disappeared on follow-up echocardiography.</P>
Biphasic RLR-IFN-β response controls the balance between antiviral immunity and cell damage.
Hwang, Sun-Young,Hur, Kye-Yeon,Kim, Jeong-Rae,Cho, Kwang-Hyun,Kim, Seung-Hwan,Yoo, Joo-Yeon Williams Wilkins 2013 JOURNAL OF IMMUNOLOGY Vol.190 No.3
<P>In RNA virus-infected cells, retinoic acid-inducible gene-I-like receptors (RLRs) sense foreign RNAs and activate signaling cascades to produce IFN-alpha/beta. However, not every infected cell produces IFN-alpha/beta that exhibits cellular heterogeneity in antiviral immune responses. Using the IFN-beta-GFP reporter system, we observed bimodal IFN-beta production in the uniformly stimulated cell population with intracellular dsRNA. Mathematical simulation proposed the strength of autocrine loop via RLR as one of the contributing factor for biphasic IFN-beta expression. Bimodal IFN-beta production with intracellular dsRNA was disturbed by blockage of IFN-alpha/beta secretion or by silencing of the IFN-alpha/beta receptor. Amplification of RLRs was critical in the generation of bimodality of IFN-beta production, because IFN-beta(high) population expressed more RLRs than IFN-beta(low) population. In addition, bimodality in IFN-beta production results in biphasic cellular response against infection, because IFN-beta(high) population was more prone to apoptosis than IFN-beta(low) population. These results suggest that RLR-mediated biphasic cellular response may act to restrict the number of cells expressing IFN-beta and undergoing apoptosis in the infected population. The Journal of Immunology, 2013, 190: 1192-1200.</P>