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Min Jee Kim,Sun Ok Park,Ye Seul Hong,Eun A Park,Yu Bin Lee,Byung-Ok Choi,Kyung-Ah Lee,Eun Jeong Yu,Inn Soo Kang 대한의학유전학회 2022 대한의학유전학회지 Vol.19 No.1
Purpose: Preimplantation genetic testing for monogenic disorders (PGT-M) has been successfully used to prevent couples with monogenic disorders from passing them on to their child. Charcot–Marie–Tooth Disease (CMT) is a genetic disorder characterized by progressive extremity muscle degeneration and loss of sensory function. For the first time in Korea, we report our experience of applying single nucleotide polymorphism genotyping and karyomapping for PGT-M of CMT disease. Materials and Methods: Prior to clinical PGT-M, preclinical tests were performed using genotypes of affected families to identify informative single-nucleotide polymorphisms associated with mutant alleles. We performed five cycles of in vitro fertilization PGT-M in four couples with CMT1A, CMT2A, and CMT2S in CHA Fertility Center, Seoul Station. Results: From July 2020 through August 2021, five cycles of PGT-M with karyomapping in four cases with CMT1 and CMT2 were analyzed retrospectively. A total of 17 blastocysts were biopsied and 15 embryos were successfully diagnosed (88.2%). Ten out of 15 embryos were diagnosed as unaffected (66.7%). Five cycles of PGT-M resulted in four transfer cycles, in which four embryos were transferred. Three clinical pregnancies were achieved (75%) and the prenatal diagnosis by amniocentesis for all three women confirmed PGT-M of karyomapping. One woman delivered a healthy baby uneventfully and two pregnancies are currently ongoing. Conclusion: This is the first report in Korea on the application of karyomapping in PGT-M for CMT patients. This study shows that karyomapping is an efficient, reliable and accurate diagnostic method for PGT-M in various types of CMT diseases.
지용선(Jee Young-sun),이경원(Lee Kyung-won),옥선종(Ok Sun-jong) 한국전자상거래학회 2001 전자상거래학회지 Vol.2 No.1
With the rapid growth of Internet users, a lot of applications have been introduced in the real world.<BR> Despite the tremendous growth in electronic commerce, there are numerous shortcomings in the criteria that can serve as a market guide to both Internet shopping mall businesses and consumers. A few institutional entities that recognize and certify outstanding shopping malls and online shops are limited to extending their recognition or certification to only the few leading shopping malls.<BR> In this article, we focus on the Internet business model and evaluation method of it. Each of many researches has concentrated on the classification of the Internet business model with a single criterion such as business activity, transaction partner, revenue source, product types, etc. However, the Internet business is performed with integration of various factors affecting the success of the business. Therefore, it is need to have an integrated business model for the Internet business. In this article, we suggest a conceptual model for it.
고체상 정제 및 HPLC/PDA에 의한 영유아식 중 나이아신의 분석
홍지은(Jee Eun Hong),김미란(Miran Kim),천상희(Sanghee Cheon),채정영(Jungyoung Chai),박은령(Eun Ryong Park),문춘선(Chun Sun Mun),곽인신(In-Shin Gwak),김옥희(Ok-Hee Kim),이광호(Kwang Ho Lee) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.3
영유아식 중 나이아신의 기기분석법을 개발하고 영ㆍ유아식 중의 나이아신의 함량을 조사하였다. 니코틴산 및 니코틴아미드에 대해 액체 추출 및 고체상 정제과정을 통해 전처리한 후 HPLC로 분석할 경우 83~104% 범위의 회수율과 1.5~3.5% 범위의 재현성을 나타내었다. 또한 260 ㎚의 파장에서 분석할 경우 0.02~10 ㎎/L 범위에서 검량선의 직선성이 1.0000 수준으로 매우 높게 나타났으며 영유아식 중의 검출한계는 0.2 ㎎/㎏ 수준으로 나타났다. 총 50건의 영ㆍ유아식에 대해 나이아신 함량을 모니터링한 결과 53.5~140.3 ㎎/㎏ 수준으로 나타났으며 3회 반복시험 시의 상대표준편차는 0.6~5.5% 범위로 나타나 본 방법이 나이아신 분석에 유용한 방법임을 확인하였다. This study was performed to establish a rapid and simple analytical method for niacin (nicotinic acid and nicotinamide) using HPLC. A pretreatment method for the extraction and clean-up of niacin in infant formula sample and an instrumental condition for HPLC were optimized. Niacin was extracted by 5 mM hexanesulfonate with ultrasonication for 30 min. For the clean-up of the sample, the extract was applied to a HLB cartridge, and then niacin was eluted from the cartridge using 5 mL of 80% methanol after washing with 5 mL of n-hexane. The total recoveries were 83~104% and relative standard deviation were in the range of 1.5~3.5% during the extraction and clean-up process. Niacin was determined by gradient elution with sodium hexanesulfonate/methanol buffer as a mobile phase and a photodiode array detector (260 ㎚). It showed a high linearity between the content of niacin and the peak area (r²=1.000) in the range of 0.02~10.0 ㎎/L of nicotinic acid and nicotinamide. The detection limit was 0.02 ㎎/L (0.2 ㎎/㎏ in the sample). The method was successfully applied for the determination of niacin in infant formula. Total niacin contents were in the range of 53.5~140.3 ㎎/㎏.
정맥내 통증 자가조절법에 의한 술후 통증관리 1,590예에 대한 분석
송선옥(Sun Ok Song),지대림(Dae Lim Jee),구본업(Bon 대한통증학회 1996 The Korean Journal of Pain Vol.9 No.2
N/A Background: We started postoperative pain management service using an intravenous patient-controlled analgesia(IV-PCA, PCA), which is known as convenient and effective an- algesic method. In this report, we describe the efficacy and safety of PCA and the experi- ence of developing an acute pain service to treat postoperative pain using a PCA. Methods: Practices of an acute pain service were started at a ward for general surgery after preparation of the standardized protocols for PCA. In each patient, PCA was con- nected following administration o;. initial loading doses of analgesics at recovery room after operation. All patients were checked by acute pain service team once or twice daily. The scope of acute pain service was gradually spread to other departments such as ortho- pedic, thoracic, obstetric and gynecologic departments by requests of patients or surgeons. We managed 1,590 patients during first 22 months. Among them, nine hundred seventy two cases were prospectively evaluated for their analgesic efficacy and side effects of PCA. Results: The number of patients was increased day by day. The most common type of operation was gastrectomy(21.6%). Cominonly used analgesics were nalbuphine(59%) and morphine(37%,). The mean duration of PCA attachment was 3.3 days. The degree of anal- gesia on operation day was good in 44.8#k and tolerable in 52.6% of patients. Only 3.9% of patients complained severe pain during their postoperative periods. One elderly patient ex- perienced respiratory depression(0.06%) owing to accidental misuse of PCA by his relatives. Overall patient's satisfaction was over 93%. Conclusions: According to our experiences, we conclude that PCA is an effective, relative- ly safe and highly satisfactory method for postoperative pain management. Because of these advantages of PCA, the creation of our acute pain service using a PCA was success- ful and expanded rapidly.
Production Frequency, Semantic Typicality, and Familiarity of Noun Exemplars for Elderly Adults
성지은(Jee Eun Sung),모경옥(Kyeong Ok Mo),이지선(Ji Sun Lee),심현섭(Hyun Sub Sim) 한국언어청각임상학회 2014 Communication Sciences and Disorders Vol.19 No.2
배경 및 목적: 본 연구는 향후 신경언어장애군을 대상으로 이름대기 중재 프로그램 개발을 위한 기초자료를 확보하는 목적으로, 명사 범주별 본보기 산출빈도, 의미전형성 및 친숙도에 대해 조사하였다. 방법: 실험 1에서는 전문가 및 보호자 75명을 대상으로 이름대기 중재에 필요한 명사 범주 총 17개를 중요한 순서대로 우선순위를 정하도록 하였다. 실험 2에서는 노년층 45명을 대상으로 17개의 명사 범주에 대한 생성이름대기를 실시하였다. 실험 3에서는 실험 2에 참여하지 않은 노년층 78명을 대상으로 실험 1에서 우선순위 3순위 안에 포함되는 6개 범주에서 정리된 각 항목별로 의미전형성 및 친숙도에 대한 7점 척도 평정을 실시하였다. 결과: 실험 1에 따르면, 우선순위가 가장 높은 범주는 음식(요리)인 것으로 나타났으며, 실험 2에서는 가장 많은 본보기 산출빈도를 보인 범주는 신체부위였다. 실험 3에서는 산출 본보기에 대한 의미전형성 및 친숙도 평정척도를 표준화점수(Z-score)로 환산하여 기초자료를 제시하였으며, 의미전형성 및 친숙도는 강한 정적 상관관계를 보였다. 논의 및 결론: 본 연구는 향후 신경언어장애군의 이름대기 중재 프로그램 개발을 위해 명사 범주별 속성에 관한 노년층 기초자료를 제공함으로써 임상적 활용가치가 높을 것으로 기대한다. Objectives: This study seeks to provide normative data on the production frequency and semantic typicality and familiarity of noun exemplars by semantic category for elderly individuals. Methods: A total of 198 individuals participated in the study. In Experiment 1, participants were categorized into two groups: professionals involved in dementia care and primary care-givers of patients with dementia. They ranked 17 semantic categories based on the priority of the stimuli required for naming treatment. Forty-five normal elderly individuals (NEIs) participated in Experiment 2, in which they were administered a generative naming task. Seventy-eight NEIs participated in Experiment 3, where they rated the semantic typicality and familiarity of each noun exemplar obtained from Experiment 2. Results: Experiment 1 revealed that ‘food (dish)’ was ranked as a top priority followed by ‘clothes’, ‘fruits’, and ‘body parts’. In Experiment 2, ‘body parts’ generated the highest mean of production frequency among 17 semantic categories. Results from Experiment 3 suggested that the semantic typicality was positively and strongly correlated with the familiarity ratings. Conclusion: These results are clinically important and meaningful given that they provide preliminary guidelines to select stimuli for the naming treatment of individuals with neurogenic language disorders.