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Syringic acid prevents skin carcinogenesis via regulation of NoX and EGFR signaling
Ha, Su Jeong,Lee, Jangho,Park, Joon,Kim, Young Ho,Lee, Nam Hyouck,Kim, Young Eon,Song, Kyung-Mo,Chang, Pahn-Shick,Jeong, Chul-Ho,Jung, Sung Keun Elsevier 2018 Biochemical pharmacology Vol.154 No.-
<P><B>Abstract</B></P> <P>Validation of nutraceutical and pharmaceutical targets is essential for the prediction of physiological and side effects. Epidemiologic evidence and molecular studies suggest that non-melanoma skin cancer is directly associated with excessive exposure to ultraviolet (UV) radiation. The aim of the present study was to evaluate the inhibitory effects of syringic acid on UVB-induced signaling and skin carcinogenesis, and determine the molecular targets. Treatment of human epidermal keratinocytes (HaCaT) cells with syringic acid resulted in the suppression of UVB-induced cyclooxygenase-2, matrix metalloproteinase-1, and prostaglandin E<SUB>2</SUB> expression as well as activator protein-1 activity. Moreover, syringic acid inhibited the UVB-induced phosphorylation of mitogen-activated protein kinases and Akt signaling pathways as well as epidermal growth factor receptor (EGFR). Syringic acid treatment further inhibited intracellular reactive oxygen species and protein-tyrosine phosphatase-κ activity, a regulator of EGFR activation. Syringic acid and the antioxidant N-acetyl-<SMALL>L</SMALL>-cysteine inhibited UVB-induced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity. <I>In vivo</I>, pretreatment of mouse skin with syringic acid significantly suppressed UVB-induced skin tumor incidence in a dose-dependent manner. Overall, these results indicate that syringic acid exerts potent chemopreventive activity in skin carcinogenesis mainly by inhibition of the Nox/PTP-κ/EGFR axis. Syringic acid might serve as an effective chemopreventive and therapeutic agent against UVB-mediated skin cancer.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Ha, Su Jeong,Lee, Jangho,Song, Kyung-Mo,Kim, Young Ho,Lee, Nam Hyouck,Kim, Young-Eon,Jung, Sung Keun The Royal Society of Chemistry 2018 Food & Function Vol.9 No.4
<P>This study evaluated the use of ultrasonication to extract <I>Lespedeza cuneata</I> as a potential nutraceutical for preventing vascular inflammation. Ultrasonicated <I>L. cuneata</I> extract (ULCE) was prepared using 20% ethanol and 2 h of ultrasonication at room temperature, and its effects were investigated using relevant <I>in vitro</I> and <I>in vivo</I> models. ULCE suppressed tumor necrosis factor-alpha (TNF-α)-induced adhesion capacity, vascular cell adhesion protein 1 (VCAM-1) expression, and nuclear factor kappa-B (NF-κB) activity in human umbilical vein endothelial cells (HUVECs). ULCE also suppressed TNF-α-induced NF-κB signaling pathways and p65 translocation from the cytosol to the nucleus, as well as the mRNA expression of IL-1β, IL-6, and TNF-α in HUVECs. Oral administration of ULCE suppressed TNF-α-induced monocyte infiltration into the intima and VCAM-1 expression, as well as the IL-1β, IL-6, TNF-α, and monocyte chemoattractant protein-1 (MCP-1) mRNA expression in the main artery in mice. Among the compounds identified in the hydrolyzed ULCE, quercetin exhibited the strongest inhibitory effect against TNF-α-induced cell adhesion capacity. These results demonstrate that ULCE contains potent preventive factors against early atherosclerosis, which act by suppressing the NF-κB and VCAM-1 signaling axis.</P>
카드뮴 분석용 홍합(Mytilus coruscus) 표준물질의 균질성 및 안정성 시험평가
이하은 ( Ha-eun Lee ),이장호 ( Jangho Lee ),정다위 ( David Chung ),이수용 ( Soo Yong Lee ),박기완 ( Ki-wan Park ),심규영 ( Kyu-young Shim ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.11
In this study, the KS A ISO Guide 35 was applied to develop analytical standards for heavy metal cadmium using the Korean mussel (Mytilus coruscus) and to evaluate the homogeneity and stability of the sample. Some of the crucial characteristics that reference materials must consist include homogeneity and stability of both intra- and inter-bottles. We tested homogeneity using ANOVA analysis and short-term stability using regression analysis. The variations of cadmium concentrations did not significantly differ between intra- and inter-bottles (F=0.41, p=0.90). For short-term stability verification, cadmium analysis results were not statistically significant as a result of the regression analysis (significance F=0.51, p=0.53). This suggests that we can not dismiss the null hypothesis that there is no significant variation in concentrations of cadmium over time. These results indicated that the cryogenic-milling process has statistically proven the short-term stability for materials from mussels in the chemical analysis of cadmium. Therefore, we propose that the Korean mussel’s reference material developed for the proficiency test could be used as a tool to evaluate reliability and consistency in laboratories.
Lee, Jangho,Ha, Su Jeong,Lee, Hye Jin,Kim, Min Jung,Kim, Jin Hee,Kim, Yun Tai,Song, Kyung-Mo,Kim, Young-Jun,Kim, Hyun Ku,Jung, Sung Keun The Royal Society of Chemistry 2016 Food & Function Vol.7 No.7
<▼1><▼1><P><I>Tremella fuciformis</I> Berk (TFB) prevent LPS-mediated inflammation by inhibition of NF-κB and MAPKs pathways.</P></▼1><▼2><P><I>Tremella fuciformis</I> Berk (TFB) has long been used as a traditional medicine in Asia. Although TFB exhibits antioxidant and anti-inflammatory effects, the mechanisms of action responsible have remained unknown. We confirmed the anti-inflammatory effects of <I>Tremella fuciformis</I> Berk extract (TFE) in RAW 264.7 cells and observed significantly suppressed LPS-induced iNOS/NO and COX-2/PGE2 production. TFE also suppressed LPS-induced IKK, IkB, and p65 phosphorylation, as well as LPS-induced translocation of p65 from the cytosol. Additionally, TFE inhibited LPS-induced phosphorylation of MAPKs. In an acute inflammation study, oral administration of TFE significantly inhibited LPS-induced IL-1β, IL-6 and TNF-α production and iNOS and COX-2 expression. The major bioactive compounds from TFB extract were identified as gentisic acid, protocatechuic acid, 4-hydroxybenzoic acid, and coumaric acid. Among these compounds, protocatechuic acid showed the strongest inhibitory effects on LPS-induced NO production in RAW 264.7 cells. Overall, these results suggest that TFE is a promising anti-inflammatory agent that suppresses iNOS/NO and COX-2/PGE2 expression, as well as the NF-κB and MAPK signaling pathways.</P></▼2></▼1>
Hydrogel Nanospike Patch as a Flexible Anti-Pathogenic Scaffold for Regulating Stem Cell Behavior
Park, Sunho,Park, Hyun-Ha,Sun, Kahyun,Gwon, Yonghyun,Seong, Minho,Kim, Sujin,Park, Tae-Eun,Hyun, Hoon,Choung, Yun-Hoon,Kim, Jangho,Jeong, Hoon Eui American Chemical Society 2019 ACS NANO Vol.13 No.10
<P>Vertically aligned nanomaterials, such as nanowires and nanoneedles, hold strong potential as efficient platforms onto which living cells or tissues can be interfaced for use in advanced biomedical applications. However, their rigid mechanical properties and complex fabrication processes hinder their integration onto flexible, tissue-adaptable, and large-area patch-type scaffolds, limiting their practical applications. In this study, we present a highly flexible patch that possesses a spiky hydrogel nanostructure array as a transplantable platform for enhancing the growth and differentiation of stem cells and efficiently suppressing biofilm formation. <I>In vitro</I> studies show that the hydrogel nanospike patch imposes a strong physical stimulus to the membranes of stem cells and enhances their osteogenic, chondrogenic, and adipogenic differentiation and the secretion of crucial soluble factors without altering cell viability. At the same time, the array exhibits effective bactericidal properties against Gram-positive and Gram-negative bacteria. <I>In vivo</I> studies further demonstrate that the flexible hydrogel patch with its spiky vertical nanostructures significantly promotes the regeneration of damaged cranial bone tissues while suppressing pathogenic bacterial infections in mouse models.</P> [FIG OMISSION]</BR>