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상수원수 수질변화에 따른 전오존 처리효과 및 경제성 평가
최동훈 ( Dong Hoon Choi ),박진식 ( Jin Sik Park ),문추연 ( Choo Yeun Moon ),이재용 ( Jae Yong Lee ),유동춘 ( Dong Choon Ryu ),장성호 ( Seong Ho Jang ),권기원 ( Ki Won Kwon ),이수애 ( Soo Ae Lee ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.4
This study, changes in raw water quality is to indicate on the efficiency of ozone treatment of each pollutant as compared to derive the appropriate operating measures. The appropriate selection for injection rate of pre-ozone and did not inject pre-ozone assess changes in the water. When good water quality, you not injected of pre-ozone to evaluate the economic efficiency of electricity and put the most cost-effective ozone concentration were evaluated. Evaluation remove organic matter and chlorophyll-a concentration level in experiments with each factor of the water DOC> 2.5㎎ / L, THMFP> 70㎍ / L, Chl-a> 30㎎/㎥or less constant process, if you do not need to put pre-ozone showed little impact. It also does not put you in pre-ozone appropriate produce enough power rate savings was calculated as approximately 90 million won. Ability to remove organic materials and the ability to produce disinfection byproducts, and cost-effective decisions by considering the concentration of injection if pre-ozone 1 mg/L was investigated by the appropriate concentration of ozone injection.
Won Hee Jang(장원희),Sang-Jin Kim(김상진),Young Joo Jeong(정영주),Hee Jae Jun(전희재),Il Soo Moon(문일수),Dae-Hyun Seog(석대현) 한국생명과학회 2012 생명과학회지 Vol.22 No.12
KIF5/Kinesin-I는 경쇄(light chain)를 통하여 결합함으로써 다양한 운반체들을 미세소관을 따라 운반한다. Kinesin light chains (KLCs)은 tetratricopeptide repeat (TPR) 영역을 매개로 운반체와 결합한다. 현재까지 KLCs와 결합하는 많은 운반체들이 확인되었으나 KLCs가 어떻게 특정운반체를 인식하여 결합하는지는 아직 확실히 밝혀지지 않았다. 본 연구에서 KLC1의 TPR 영역과 결합하는 단백질을 분리하기 위하여 효모 two-hybrid system을 이용하여 탐색한 결과 amyloid precursor protein (APP)과 결합하는 것으로 보고된 protein interacting with APP tail 1 (PAT1)을 분리하였다. KLC1은 PAT1의 C-말단 부위와 결합하며, PAT1은 KLC1의 TPR 영역을 포함한 부위와 결합함을 효모 two-hybrid assay로 확인하였다. 또한 PAT1는 KLC2와도 결합하였지만 kinesin heavy chains (KHCs)인 KIF5A, KIF5B, KIF5C와는 결합하지 않았다. 단백질간 결합은 glutathione S-transferase (GST) pull-down assay와 공동면역침강으로도 확인하였다. 생쥐의 뇌 파쇄액을 PAT1 항체와 APP 항체로 면역침강을 행한 결과 KLC와 KHCs가 같이 침강하였다. 이러한 결과들은 PAT1이 Kinesin-I와 APP 포함 소포간의 상호작용을 매개한다는 것을 시사한다. A conventional kinesin, KIF5/Kinesin-I, transports various cargoes along the microtubule through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) interact with many different cargoes using their tetratricopeptide repeat (TPR) domain, but the mechanism underlying recognition and binding of a specific cargo has not yet been completely elucidated. We used the yeast two-hybrid assay to identify proteins that interact with the TPR domain of KLC1. We found an interaction between the TPR domain of KLC1 and an amyloid precursor protein (APP)-binding protein PAT1 (protein interacting with APP tail 1). The yeast two-hybrid assay demonstrated that the TPR domain-containing region of KLC1 mediated binding to the C-terminal tail region of PAT1. PAT1 also bound to KLC2 but not to kinesin heavy chains (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. These protein-protein interactions were also observed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti-PAT1 antibody as well as anti-APP antibody co-immunoprecipitated KLC and KHCs associated with PAT1 from mouse brain extracts. These results suggest that PAT1 could mediate interactions between Kinesin-I and APP containing vesicles.
A Study of Thyroid Function in Partial Thyroxine-Binding Globulin Deficiency
Jae Won Lee,Jang Yong Jin,Jungho Lee,Dong Hwan Lee,Yong Hee Hong 순천향대학교 순천향의학연구소 2015 Journal of Soonchunhyang Medical Science Vol.21 No.2
Objective: It is generally thought that thyroxine-binding globulin (TBG)-deficient individuals are euthyroid and do not require treatment. However, there have been case reports of TBG deficiency combined with hypothyroidism. The purpose of this study was to investigate the relationship between TBG deficiency and thyroid function. Methods: We reviewed the medical records of 32 patients diagnosed with TBG deficiency between 1997 and 2008 in Soonchunhyang University Seoul Hospital. All were partial TBG deficiency. Eighteen patients had combined hypothyroidism, and 14 patients had normal thyroid function. We compared the TBG, thyroid-stimulating hormone, free thyroxine, and total triiodothyronine levels between these 2 groups. Eighteen patients with TBG deficiency with hypothyroidism started thyroxine medication and continued for 2-3 years. After, they were followed up with thyroid function tests after discontinuing medication for 4 weeks at 2-3 years of age. Results: The TBG level in TBG deficiency with hypothyroidism patients was significantly lower than that in TBG deficiency with normal thyroid function (4.43±2.22 mg/L vs. 6.23±1.81 mg/L; P=0.02). The percent TBG compared with normal mean TBG level according to age in the hypothyroidism patients was also significantly lower than that of patients with normal thyroid function (13.42%±6.92% vs. 19.08%±4.87%; P=0.014). Sixteen of 18 patients diagnosed with TBG deficiency with hypothyroidism showed persistent hypothyroidism at 2-3 years of age. Conclusion: We conclude that TBG-deficient patients should be observed closely and undergo thyroid function testing in order not to miss hypothyroidism. More investigations of TBG deficiency and thyroid function are needed in the future.
Jang, Kyung Ku,Lee, Zee-Won,Kim, Bityeoul,Jung, Young Hyun,Han, Ho Jae,Kim, Myung Hee,Kim, Byoung Sik,Choi, Sang Ho American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.41
<P>The marine bacterium Vibrio vulnificus causes food-borne diseases, which may lead to life-threatening septicemia in some individuals. Therefore, identifying virulence factors in V. vulnificus is of high priority. We performed a transcriptome analysis on V. vulnificus after infection of human intestinal HT29-methotrexate cells and found induction of plpA, encoding a putative phospholipase, VvPlpA. Bioinformatics, biochemical, and genetic analyses demonstrated that VvPlpA is a phospholipase A(2) secreted in a type II secretion system-dependent manner. Compared with the wild type, the plpA mutant exhibited reduced mortality, systemic infection, and inflammation in mice as well as low cytotoxicity toward the human epithelial INT-407 cells. Moreover, plpA mutation attenuated the release of actin and cytosolic cyclophilin A from INT-407 cells, indicating that VvPlpA is a virulence factor essential for causing lysis and necrotic death of the epithelial cells. plpA transcription was growth phase-dependent, reaching maximum levels during the early stationary phase. Also, transcription factor HlyU and cAMP receptor protein (CRP) mediate additive activation and host-dependent induction of plpA. Molecular biological analyses revealed that plpA expression is controlled via the promoter, P-plpA, and that HlyU and CRP directly bind to P-plpA upstream sequences. Taken together, this study demonstrated that VvPlpA is a type II secretion system-dependent secretory phospholipase A(2) regulated by HlyU and CRP and is essential for the pathogenicity of V. vulnificus.</P>