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Plant-Parasitic Nematodes of Regulatory Importance to Florida, USA
Janete A. Brito 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
Since 1956 the state of Florida has been implementing Nematology regulatory programs to protect high value crops such as Citrus spp and ornamental plants. The discovery of Radopholus similis, which causes a severe disease called “spreading decline” of citrus, during the 1950s, prompted the state to develop and implement strict phytosanitary and regulatory measures, including quarantine and a citrus nursery certification program to avoid further dissemination of the nematode. This also included two other major citrus pathogens, Pratylenchus coffeae and Tylenchulus semipenetrans. Also, ornamental and other plant nurseries certification programs were implemented to promote the export of plants to both national and international markets. These markets have restrictions against the movement of R. similis and other plant-parasitic nematodes that occur in Florida. Other plant-parasitic nematodes present in Florida, not regulated internally but regulated by other states and countries include Rotylenchulus reniformis and Belonolaimus longicaudatus. Regarding root-knot nematodes occurring in Florida, only Meloidogyne enterolobii is of regulatory concern because its ability to overcome the resistance of Mi-1, N, Rk, Tabasco and in omato, bell pepper, cowpea, sweet pepper and soybean, respectively, as well as other unidentified genes in soybean cultivars. The identification of M. enterolobii using morphological and morphometric characters, molecular markers, such as isozyme phenotypes (esterase and malate dehydrogenase) and mtDNA (C2F3/1108 primer set) will be discussed.
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Francisco-Javier Jiménez-Balderas,Janete García-Jaimes,Rita Ríos,Abraham Zonana-Nacach,Raquel Tapia-,Nayeli Villanueva,Patricia Méndez-Samperio,Jorge-Luis de-la-Rosa-Arana 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.3
Purpose: Since few reports had been published on the prevalence of toxocariasis in ankylosing spondylitis (AS)patients with acute non-granulomatous anterior uveitis (ANGAU), the aim of this work was to determine thepresence of antibodies against Toxocara canis in AS patients with ANGAU. Methods: Thirty-six patients (14 female and 22 male) with AS were enrolled in the study. The history of ANGAUwas accepted only if diagnosed by an ophthalmologist. The detection of IgG antibodies to T. canis was determinedby enzyme-linked immunosorbent assay. In addition, antibodies to Ascaris lumbricoides were alsotested to verify non-specific reactions. Results: The prevalence of ANGAU in the AS patients was 58% (21 / 36), and 38% (8 / 21) of the patients withANGAU were positive for antibodies to Toxocara, while 7% (1 / 15) of AS patients without ANGAU were positivefor T. canis (p = 0.038, two tails; mid-p exact). No antibodies were detected to A. lumbricoides antigens inthe serum samples of patients with AS. Conclusions: These data suggest that the seroprevalence of antibodies to T. canis is high in Mexican patientswith AS-associated uveitis, suggesting a chronic asymptomatic toxocariosis, which could be associated withthe pathogenesis of ANGAU; however, further larger-scale studies are needed to confirm this observation.
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Matheus Soldatelli,Álvaro de Oliveira Franco,Felipe Picon,Juliana Ávila Duarte,Ricardo Scherer,Janete Bandeira,Maxciel Zortea,Iraci Lucena da Silva Torres,Felipe Fregni,Wolnei Caumo 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.1
Background: Resting-state functional connectivity (rs-FC) may aid in understanding the link between painmodulating brain regions and the descending pain modulatory system (DPMS) in fibromyalgia (FM). This study investigated whether the differences in rs-FC of the primary somatosensory cortex in responders and non-responders to the conditioned pain modulation test (CPM-test) are related to pain, sleep quality, central sensitization, and the impact of FM on quality of life. Methods: This cross-sectional study included 33 females with FM. rs-FC was assessed by functional magnetic resonance imaging. Change in the numerical pain scale during the CPM-test assessed the DPMS function. Subjects were classified either as non-responders (i.e., DPMS dysfunction, n = 13) or responders (n = 20) to CPM-test. A generalized linear model (GLM) and a receiver operating characteristic (ROC) curve analysis were performed to check the accuracy of the rs-FC to differentiate each group. Results: Non-responders showed a decreased rs-FC between the left somatosensory cortex (S1) and the periaqueductal gray (PAG) (P < 0.001). The GLM analysis revealed that the S1-PAG rs-FC in the left-brain hemisphere was positively correlated with a central sensitization symptom and negatively correlated with sleep quality and pain scores. ROC curve analysis showed that left S1-PAG rs-FC offers a sensitivity and specificity of 85% or higher (area under the curve, 0.78, 95% confidence interval, 0.63–0.94) to discriminate who does/does not respond to the CPM-test. Conclusions: These results support using the rs-FC patterns in the left S1-PAG as a marker for predicting CPM-test response, which may aid in treatment individualization in FM patients.
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Social vulnerability and COVID-19 in Maringá, Brazil
Matheus Pereira Libório,Oseias da Silva Martinuci,Patrícia Bernardes,Natália Cristina Alves Caetano Chaves Krohling,Guilherme Castro,Henrique Leonardo Guerra,Eduardo Alcantara Ribeiro,Udelysses Janete 대한공간정보학회 2023 Spatial Information Research Vol.31 No.1
This research explores the relationship between COVID-19 and social vulnerability on an intra-urban scale. For this, two composite indicators of social vulnerability have been constructed. The composite indicator constructed by the Benefit-of-the-Doubt considers spatial heterogeneity. It weakly captures the conceptually most significant individual indicator of social vulnerability (R=-0.39), as it overestimates the above-average performance sub-indicators. The composite indicator constructed by the Principal Component Analysis considers that the sub-indicators have the same weights in different census tracts, resulting in a highly consistent composite indicator as a multidimensional phenomenon concept (R=-0.93). These findings allow reaching four conclusions. First, the direction and strength of correlations associated with COVID-19 are sensitive to the method employed to construct the composite indicator and not just the geographic scale and space. Second, Medium and High social vulnerability census tracts concentrate 97% of the population but only 93% of COVID-19 cases and deaths. Third, people living in census tracts of None and Low social vulnerability are 3.87 and 2.13 times more likely to be infected or die from COVID-19. Fourth, policies to combat COVID-19 in the study area should prioritize older populations regardless of their social conditions.
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Fabiana América Silva Dantas de Souza,Amanda Emmanuelle Sales,Pablo Eugênio Costa e Silva,Raquel Pedrosa Bezerra,Germana Michelle de Medeiros e Silva,Janete Magali de Araújo,Galba Maria de Campos Taka 한국고분자학회 2016 Macromolecular Research Vol.24 No.7
The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.
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Janet Alejandra Gutierrez-Uribe,Mauricio Salinas-Santander,Delia Serna-Guerrero,Sergio Roman Othon Serna-Saldivar,Ana Maria Rivas-Estilla,Clara Patricia Rios-Ibarra 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1
MicroRNAs (miRNAs) are small molecules of 19–23 nucleotides of RNA that act as regulators of the expression of proteins in eukaryotic cells. Currently, the participation of miRNAs in the development of different types of cancer has been observed. To evaluate the inhibitory effect of kaempferol-3-O-glycoside on the expression of oncological biomarkers, miR31 and miR92a in a colon cancer cell line (RKO) were analyzed. Cells were cultured and treated with 1 mM kaempferol-3-O-glycoside isolated from black bean. Expression levels of miR31 and miR92a were evaluated by real-time PCR using TaqMan probes; in addition, two oncogenes (KRAS and c-MYC) and two tumor suppressors (AMP-activated protein kinase [AMPK] and adenomatous tumors of polyposis coli [APC]) were quantified to validate the biological effects; normalization of expression levels were carried out by 2−ΔΔCt. Results were analyzed by one-way ANOVA. The expression levels of miR31, miR92a, KRAS oncogene, and the c-MYC transcription factor were subexpressed upon 72 h post-treatment with kaempferol-3-O-glycoside compared with the control without treatment (P < .05); in contrast, the tumor suppressor genes AMPK (∼4.85, P = .005) and APC (∼2.71, P = .066) tumor suppressors genes were overexpressed. Our results showed the inhibitory effect of isolated black bean flavonoid kaempferol-3-O-glycoside on cancer biomarkers: miR31 and miR92a; based on our results, this flavonoid may have interesting nutritional, therapeutic, and/or prophylactic applications to combat colon cancer.
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Collaborative Information Technology(CIT) Applications in Post-Disaster Public-Private Partnerships
Janet Dilling,Audrey Heffron-Casserleigh 위기관리 이론과 실천 2008 Crisisonomy Vol.4 No.2
The last several years of the Atlantic Hurricane Season have been some of the busiest and most destructive in history. Although paling in human terms in comparison to the Asian Tsunami with its sobering loss of life, a series of Atlantic Hurricanes from 2004 – 2008 have generated some of the largest response and recovery efforts in U.S. history with damage estimates exceeding $100 billion. This paper will discuss two unique public-private partnerships, which operating as computer supported virtual organizations helped redefine state and federal response relating to post disaster construction and temporary housing issues in a disaster. In doing so, the paper will expand collaborative information technology (CIT). The term CIT is used to denote asynchronous technology that provides support for coordination and collaboration through computer supported communication. Although CIT, closely related Computer Mediated Communication (CMC), and Computer Supported Cooperative Work (CSCW) are usually associated with constructing or managing interorganizational communication, this paper will explore how web based technology bridged the information gap between government, the private sector, and victims left homeless by a series of catastrophic hurricanes.
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Janet Lee,Chang Geun Lee,Kyo-Won Lee,Chang-Woo Lee 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.12
BubR1 mitotic checkpoint kinase monitors attachment of microtubules to kinetochores and links regulation of the chromosome-spindle attachment to mitotic checkpoint signaling. Defects in BubR1-mediated signaling severely perturb checkpoint control and are linked to diseases such as cancer. Studies using BubR1 mouse models suggest that BubR1 activities prevent premature aging and infertility. In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes loss of the differentiation potential and induction of replicative senescence. These effects occur independently of p16INK4A expression and may involve DNA methylation. Our results reveal a new and unsuspected feature of BubR1 expression in regulation of adult stem cell differentiation. BubR1 mitotic checkpoint kinase monitors attachment of microtubules to kinetochores and links regulation of the chromosome-spindle attachment to mitotic checkpoint signaling. Defects in BubR1-mediated signaling severely perturb checkpoint control and are linked to diseases such as cancer. Studies using BubR1 mouse models suggest that BubR1 activities prevent premature aging and infertility. In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes loss of the differentiation potential and induction of replicative senescence. These effects occur independently of p16INK4A expression and may involve DNA methylation. Our results reveal a new and unsuspected feature of BubR1 expression in regulation of adult stem cell differentiation.
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