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Janet Lee,Chang Geun Lee,Kyo-Won Lee,Chang-Woo Lee 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.12
BubR1 mitotic checkpoint kinase monitors attachment of microtubules to kinetochores and links regulation of the chromosome-spindle attachment to mitotic checkpoint signaling. Defects in BubR1-mediated signaling severely perturb checkpoint control and are linked to diseases such as cancer. Studies using BubR1 mouse models suggest that BubR1 activities prevent premature aging and infertility. In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes loss of the differentiation potential and induction of replicative senescence. These effects occur independently of p16INK4A expression and may involve DNA methylation. Our results reveal a new and unsuspected feature of BubR1 expression in regulation of adult stem cell differentiation. BubR1 mitotic checkpoint kinase monitors attachment of microtubules to kinetochores and links regulation of the chromosome-spindle attachment to mitotic checkpoint signaling. Defects in BubR1-mediated signaling severely perturb checkpoint control and are linked to diseases such as cancer. Studies using BubR1 mouse models suggest that BubR1 activities prevent premature aging and infertility. In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes loss of the differentiation potential and induction of replicative senescence. These effects occur independently of p16INK4A expression and may involve DNA methylation. Our results reveal a new and unsuspected feature of BubR1 expression in regulation of adult stem cell differentiation.
Lee, Changsun,Shim, Sehwan,Jang, Hyosun,Myung, Hyunwook,Lee, Janet,Bae, Chang-Hwan,Myung, Jae Kyung,Kim, Min-Jung,Lee, Seung Bum,Jang, Won-Suk,Lee, Sun-Joo,Kim, Hwi-Yool,Lee, Seung-Sook,Park, Sunhoo Elsevier 2017 cytotherapy Vol.19 No.9
<P>Background aims. Mesenchymal stromal cells (MSCs) are a promising agent for treating impaired wound healing, and their therapeutic potential may be enhanced by employing extracellular matrix scaffolds as cell culture scaffolds or transplant cell carriers. Here, we evaluated the effect of human umbilical cord blood derived (hUCB)-MSCs and a porcine small intestinal submucosa (SIS)-derived extracellular matrix scaffold in a combined radiation-wound mouse model of impaired wound healing. Methods. hUCB-MSCs and SIS hydrogel composite was applied to the excisional wound of whole-body irradiated mice. Assessment of wound closing and histological evaluation were performed in vivo. We also cultured hUCB-MSCs on SIS gel and examined the angiogenic effect of conditioned medium on irradiated human umbilical vein endothelial cells (HUVECs) in vitro. Results. hUCB-MSCs and SIS hydrogel composite treatment enhanced wound healing and angiogenesis in the wound site of mice. Conditioned medium from hUCB-MSCs cultured on SIS hydrogel promoted the chemotaxis of irradiated HUVECs more than their proliferation. The secretion of angiogenic growth factors hepatocyte growth factor, vascular endothelial growth factor-A and angiopoietin-1 from hUCB-MSCs was significantly increased by SIS hydrogel, with HGF being the predominant angiogenic factor Of irradiated HUVECs. Conclusions. Our results suggest that the wound healing effect of hUCB-MSCs is enhanced by SIS hydrogel via a paracrine factor-mediated recruitment of vascular endothelial cells in a combined radiation-wound mouse model.</P>
Kyeong Won Lee,Young Jun An,Janet Lee,Ye-Eun Jung,In Young Ko,Jonghwa Jin,Ji Hoon Park,Won Kyu Lee,Kiweon Cha,Sun-Shin Cha,Jung-Hyun Lee,Hyung-Soon Yim 한국미생물학회 2022 The journal of microbiology Vol.60 No.11
Fibroblast growth factor 11 (FGF11) is one of intracrine FGFs (iFGFs), which function within cells. Unlike canonical FGFs, FGF11 remains intracellularly and plays biological roles in FGF receptor (FGFR)-independent manner. Here, we established an expression system of recombinant FGF11 proteins in E. coli and investigated whether the extracellular administration of FGF11 can activate cellular signaling. Human FGF11 has two isoforms, FGF11a and FGF11b, depending on the presence of nuclear localization sequences (NLSs) in the N-terminus. Because these two isoforms are unstable, we prepared an FGF11a-Mut by substituting three cysteine residues in the NLS with serine and FGF11b-ΔC with C-terminal truncation. The introduction of mutation in the NLS improved the solubility of FGF11 prepared from E. coli. Exogenous addition of FGF11b and FGF11b-ΔC to BALB3T3 increased cell proliferation, while FGF11a-Mut exerted no effect. FGF11b-ΔC showed higher cell proliferation activity and FGFR signaling than FGF11b. The cell-proliferating activities of FGF11b and FGF11b-ΔC were blocked by an FGFR1 inhibitor or a recombinant FGFR1, confirming the FGFR1- dependent extracellular activity of FGF11b. The analysis of circular dichroism suggested that the C-terminus of FGF11 has an α-helical structure, which may affect its interaction with FGFR1. These results suggest that the N-and C-terminus of recombinant FGF11 are involved in the activation of FGFR1. The above results provide novel insights into the function and mechanism of FGF11 that may aid the development of useful ligands for FGFR regulation.
The Matrix of Gender, Knowledge, and Writing in the Kyuhap Ch’ongso
Lee, Janet Yoon-Sun 성균관대학교 동아시아학술원 2017 Sungkyun Journal of East Asian Studies Vol.17 No.2
The concept of gendered knowledge is often examined based on the hypothesis that women’s writings deal with intimate and personal concerns in the domestic sphere, while their male counterparts are concerned with professional achievements. The spatial division between men and women in traditional Korea likely impacted the process of knowledge formation, as knowledge requires interaction with the world. Against this backdrop, the Kyuhap ch’ongso˘ (The encyclopedia of women’s daily life), written by Yi Pingho˘ gak (1759–1824), reveals conflicts and tensions in the binary structures of male and female, public and domestic, and classical and vernacular. This article therefore investigates the construction of gendered knowledge envisioned in The Encyclopedia of Women’s Daily Life and explores the positioning of the female author in collecting, classifying, and translating knowledge. It reveals how diverse constituents in this encyclopedic work have not only contributed to but also challenged the claims of gendered norms and defines how the author navigates the cultural and literary heterogeneity of knowledge that transcends the demarcation of gender.
Lee, Jong Kil,Jin, Hee Kyung,Park, Min Hee,Kim, Bo-ra,Lee, Phil Hyu,Nakauchi, Hiromitsu,Carter, Janet E.,He, Xingxuan,Schuchman, Edward H.,Bae, Jae-sung The Rockefeller University Press 2014 The Journal of experimental medicine Vol.211 No.8
<P>In Alzheimer’s disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and in AD mice, leading to defective autophagic degradation due to lysosomal depletion. Partial genetic inhibition of ASM (<I>ASM<SUP>+/−</SUP></I>) in a mouse model of familial AD (FAD; <I>amyloid precursor protein</I> [<I>APP</I>]<I>/presenilin 1</I> [<I>PS1</I>]) ameliorated the autophagocytic defect by restoring lysosomal biogenesis, resulting in improved AD clinical and pathological findings, including reduction of amyloid-β (Aβ) deposition and improvement of memory impairment. Similar effects were noted after pharmacologic restoration of ASM to the normal range in APP/PS1 mice. Autophagic dysfunction in neurons derived from FAD patient induced pluripotent stem cells (iPSCs) was restored by partial ASM inhibition. Overall, these results reveal a novel mechanism of ASM pathogenesis in AD that leads to defective autophagy due to impaired lysosomal biogenesis and suggests that partial ASM inhibition is a potential new therapeutic intervention for the disease.</P>
FEMALE DESIRE, ILLNESS, AND METAMORPHOSIS IN ‘LOVESICK SNAKE’ NARRATIVES IN SIXTEENTH-CENTURY KOREA
JANET YOON-SUN LEE 계명대학교 한국학연구원 2015 Acta Koreana Vol.18 No.2
During the seventeenth century, a surge in fictional stories ushered in an era of romance in Korean literature, and lovesickness became a topical motif. The prototype of the lovesick figure is detected in oral stories dealing with the lovesick snake (sangsa paem) in which a lovesick woman undergoes metamorphosis into a snake. This icon of the lovesick snake has endured and persisted in written and oral traditions. This research undertakes a careful investigation of this metaphor and its meanings in various textual and cultural contexts and further explores the complex relationship of the politics of female desire, death, and metamorphosis in diverse discourses. This study reveals how the grotesque, repulsive image of serpentine transformation creates a focus on horror, alienation, and victimhood in the representation of female lovesickness. Finally, con-structs of the lovesick snake are assessed and reconsidered to expose the relationship between popular discourse and written works, uncovering a literary tendency in androcentric writing practices to associate female lovesickness with sexual and erotic illness.