複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

재래한우의 혈청단백질 좌위의 유전적 다형분석

정재록,김계웅,유재영 공주대학교 자원과학연구소 2000 資源科學硏究論文集 Vol.8 No.-

The study was carried out in order to investigate the genetic polymorphism of serum protein, Albumin(Al) and Transferrin(Tf) through polyacrylamide gel electrophoresis (PAGE) in native Hanwoo. Blood samples were collected and examined from a total of 90 cattle raising in Yesan district in Chungnam province. The results obtained were as follows; 1. The distributions of genotype for Al AA and Al AB at Al loci were 96.7%(87 heads), and 3.3%(3 heads), respectively. The homozygous genotype, Al BB was not detected in this experiment. 2. The frequencies of genes for AlA and AlB were 0.983 and 0.017, respectively. Therefore, these population of Hanwoo group had been maintaining genetic equilibrium (P>0.05). 3. The distributions of genotype for Tf AA, Tf AD1, Tf AD2, Tf AE, Tf D1D1, Tf D1D2, Tf D1E Tf D2D2 Tf D2E, and Tf EE were 10.0%, 23.3%, 20.0%, 6.7%, 6.7%, 13.3%, 6.7%, 3.3%, 6.7%, and 3.3%, respectively. 4. The frequencies of genes for TfA, TfD1, TfD2, and TfE were 0.350, 0.284, 0.233, and 0.133, respectively. 5. Al loci were supposed to be controlled by to autosomal codominant allelic genes such as AlA and AlB. However, Tf loci were assumed to be controlled by four codominant allelic genes as TfA, TfD1, TfD2, and TfE.

골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해

정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.

市乳의 유통과정에 따른 細菌數의 변화

李載英,裵仁烋,鄭忠一 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

This study was carried out to investigate the quality changes of market milk taking place throughout their distribution channels. Samples from 5 different plants were collected from delivery centers, stores and milkmen. 1. Samples from 2 plants (B.D) exceeded the legal limit in their total bacteria on the 4th day. And so did others from another 3 plants (A. B. D) on the 5th day. Stores' milk showed the highest rate of consecutive increase in their total bacteria, while milkmen's the next and centers' the lowest. 2. The number of Coliform bacteria exceeded legal limit on the 4th day was found in only one sample (B), while they were not found in others. With the passing of time, they increased in milkmen's on the 5th day, and showed greater increase in stores' thereafter. 3. In all samples except one(B) from stores, psychrotrophio bacteria did not exceed in number, till the 5th day in refrigeration, the level of 10^6-10^7/ml, at which milk begins to gelatinize and change in taste. 4. All the samples but two from stores showed negative reaction in alcohol test till 5th day, and turned positive thereafter. The reactions were similar in boiling test. 5. Till the 7th day, all samples showed the specific gravity vale of 1.028-1.034, within the normal value. Titratable acidifies were within the normal value of 0.18% in all sampler, those from shops being the highest.

경부 림프절 비대의 진단에 있어서 초음파 유도하의 핵 생검의 유용성

김은경,곽진영,유재경,박정수,정웅윤,양우익,오기근 대한초음파의학회 2003 ULTRASONOGRAPHY Vol.22 No.4

6-Sigma경영혁신기법 시행에 따른 방사선과 내부고객만족도의 변화

이근옥,박재성,강충환,김미영,김승식 한국의료QA학회 2005 한국의료질향상학회지 Vol.12 No.1

Background : The concept of 'service' in a hospital is based on the quality of medical staffs who serve as customer-satisfying media, and this is what distinguishes general hospitals from other business corporations in which customer satisfaction is achieved through commercial products. Thus, the internal employee satisfaction is essential in the improvement of the 'service' and subsequent 'competitiveness' of a hospital. The purpose of this study is to establish internal marketing strategies for a general hospital through regular surveys on employee satisfaction. Method : Surveys on employee satisfaction in radiology department were conducted regularly twice a year in a university hospital with 800 beds 2001 in 2004. The subjects of this study were 35 employees who work in radiology department. The authors developed the questionnaires and the surveys were conducted initially during the first 6 months of the year. After necessary improvements were made by applying 6 Sigma techniques, subsequent surveys were conducted during the latter 6 months of the year and the degree of employee satisfaction was compared. Results : Overall satisfaction increased with a constant rate and the authors were able to assess that the hospital is being gradually stabilized. The degree of satisfaction assessed by multiple choices showed only minor changes. However, as a result of focusing on the demands put forth by the employees through the open questions, the degree of satisfaction increased gradually year after year. Conclusions : It is important to heighten the internal employee satisfaction systematically and harmoniously through the assessment on the demands of the internal customers and feedback-based communications between the management officers and the staffs. Continuous surveys on the internal employee satisfaction will serve to be valuable materials in the establishment of internal marketing strategies for a general hospital.

3교대 근로자에서 교대근무기간과 혈압 및 심박수 변동성

하미나,김재용,박정선,정호근 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

목적 : 교대근무가 심혈관계에 미치는 만성적인 영향을 살펴보기 위하여 본 연구를 수행하였다. 방법 : 4조 3교대의 빠른 순환주기의 교대근무를 하는 1개 제조업체 남성근로자 134명을 대상으로 교대근무마다 혈압 및 심박수 변동성을 측정하였다. 교란변수로서 Karasek의 직무긴장도 모델에 의거한 49항목의 직무스트레스설문지를 이용하여 직무스트레스를 측정하였고, 수면유형과 흡연, 음주력 및 과거병력 등의 사항에 관한 설문조사를 실시하였다. 반복측정 자료의 분석을 위해 mixed model을 이용한 다변량 회귀분석을 수행하여, 교대근무기간과 혈압 및 심박수 변동성과의 관련성을 살펴보았다. 결과 : 대상 근로자의 평균연령은 29 세였다(범위 25∼44). 이들 중 77.9 % 는 현재도 흡연을 하고 있었고, 50 % 는 직무긴장도 모형에서 수동형이었다. 평균 총 교대근무기간은 5.21 년(범위 5.4 개월∼10년)이었다. 수면유형검사에서는, 극단적인 아침형이나 극단적인 저녁형은 없었다. 연령, 직무스트레스, 수면유형 및 흡연을 보정한 다변량 분석에서, 혈압은 총 교대근무기간이 증가함에 따라 통계학적으로 유의하게 증가하는 소견을 보였다(p(0.05). 심박수 변동성은 저주파 및 고주파 영역 모두에서 총 교대근무기간이 증가함에 따라 유의한 감소양상을 나타냈다(p<0.05) . 결론 : 본 연구의 결과는 교대근무가 혈압을 상승시키고 심박수 변동성을 감소시키는 것으로써 심혈관계 기능에 영향을 미침을 보여주었다. Objectives : This study was performed to investigate the chronic health effect of shift-work on cardiovascular system. Methods : We checked blood pressure(BP) and heart rate variability (HRV) at every shift on 134 male workers whose shift type was 8-hour and short rotating of 3 days shift interval. As confoundings, job stress was measured by Karasek's JCQ 49 items questionnaire and circardian type was assessed by morningness-eveningness questionnaire. Smoking and alcohol drinking habit, marital status and past medical history were also acquired. We used the method of repeated measured data analysis with mixed model to show the association between shiftwork duration and BP or HRV. Results : Average age of workers was 29 years olds(range 25∼44). Among them, 77.9 % were current smokers, 50 % were the passive type of job strain in Karasek's model. Mean shiftwork duration was 5.21 years(range 5.4 months∼10 years). In circadian type, no one was definitely morning type or definitely evening type. In multivariate analyses adjusted by age, job strain, shift, circadian rhythm and smoking, blood pressure were showed significantly increasing trends according to shiftwork duration in both systolic and diastolic (p(0.05) . Heart rate variability were also showed significantly decreasing trends according to shiftwork duration in both parasympathetic and sympathetic functions (p(0.05) . Conclusions : The results of this studs suggests the chronic negative health effect of shiftwork on cardiovascular system.

대뇌 피질 신경 세포 일차 배양에서 6-hydroxydopamine에 의한 신경세포사멸의 특성

정재훈,강효정,손성향,정영기,곽병주,노재성 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 : 본 연구는 생쥐 태자 대뇌 피질 세포 일차 배양에서 파킨슨씨병의 동물 모델을 만드는데 사용되는 신경독성 물질인 6-hydroxydopamine(6-OHDA)에 의한 신경세포 사멸의 특성을 관찰한 것이다. 방 법 : 생쥐태자의 신경세포를 배양하여 6-OHDA를 처리하고 이와 더불어 여러가지 신경세포 사멸에 영향을 주는 물질을 투여하여 신경세포의 사멸을 정량적으로 분석하고 형태학적 분석을 전자현미경을 통하여 관찰하였다. 결 과 : 배양한 신경세포에 6-OHDA를 처리하면 투여 양에 비례하여 신경세포의 사멸이 증가하여 50μM 66-OHDA를 24시간 투여하여 일으킨 신경세포 사멸의 형태학적 특징은 세포질의 수축 및 염색질의 응축(nuclear chromatin condensation) 및 핵질의 fragmentation을 동반하는 고사(apoptosis) 형태이다. 이 때의 신경세포의 사멸은 항고사 물질인, 1㎍/ml cycloheximide와 caspase 억제제인 10μMzVAD-fmk에 의해 약화되었으나 글루타메이트 길항제(10μM MK-801 혹은 50μM CNQX)나 항산화제 (100μM trolox 혹은 100μM N-acetyl-cysteine)는 6-OHDA에 의한 신경세포의 사멸을 막지 못했다. 결 론 : 이상의 형태학적 및 약물학적 특징은 6-OHDA가 고사성 신경세포 사멸을 유도한다는 것을 의미하고, 항산화제들이 효과가 없다는 것과 caspase 억제제가 보호효과가 있다는 것은 6-OHDA에 의한 고사성 신경세포 사멸에는 산화적 스트레스보다는 caspase cascade와 관여된 기전이 기여한다는 것을 시사한다. 또한 6-OHDA에 의한 신경세포의 사며른 도파민성 신경세포에 선택적이지 않으며, 일차 대뇌피질 신경세포 배양 시스템이 6-OHDA에 의한 신경세포사멸인구에 사용될 수 있음을 보여준다 Objectives : We examined the patterns of cell death induced by the 6-hydroxydopamine, a selective dopaminergic toxin that used to produce Parkinson's disease model. Method : Neocortices from 14 - or 15-day-old fetal mice for neuron-glia co-cultures were used for this experiments. Results : Cortical cell cultures exposed to 10-100μM 6-hydroxydopamine for 24 hr underwent neuronal death without injuring glia. The degenerating neurons showed hallmark of apoptosis featuring cell body shrinkage, nuclear chromatin condensation and aggregation, nuclear membrane disintegration with intact plasma membrane, and prominent internucle- osomal DNA fragmentation. Neither the glutamate antagonists (10μM MK-801 and 50μM CNQX) nor antioxidants(trolox, 100μM, N-acetyl-cysteine, 100μM prevented the 6-OHDA induced neuronal injury. The death was attenuated by addition of two different anti-apoptotic agents, 1㎍/ml cycloheximide and caspase inhibitor(100μM zVAD-fmk). Conclusion : These features suggest that 6-OHDA induced apoptotic type of neuronal death in cortical neuronal culture. Considering the protective effect of caspase inhibitors, a mechanism involing caspase cascade rather than oxidative stress is responsible for the 6-OHDA-induced neuronal apoptosis. In addition, our results showed that 6-OHDA-induced apoptosis is not confined to dopaminergic neurons and the primary cortical culture system so this system is suitable for the study of 6-OHDA-induced neuronal apoptosis.

人工甘味料의 合成過程에 對한 再考

박숙자,안송자,유영자,윤정자,윤종은,이명재,정규영,황민자 梨花女子大學校 藥學硏究所 1963 梨花藥學硏究 Vol.- No.4

할로페리돌로 유도한 신경세포 고사에 대한 신경성장인자 및 인슐린 유사 성장인자의 억제효과

강효정,강지윤,곽병주,정영기,노재성 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 본 연구는 생쥐 태자의 대뇌피질 세포 일차 배양을 이용하여 할로페리돌로 유도한 신경세포의 사멸에 대해 신경 성장인자인 brain-dervied neurtrophic factor(BDNF), Neurotrophin 4/5(NT 4/5)와 인슐린 유사 성장인자인 insulin-like growth factor Ⅰ(IGF-Ⅰ)및 IGF-Ⅱ의 조절효과를 실험한 것이다. 방법: 생쥐 태자의 신경세포를 배양한 후 할로페리돌을 처리하여 신경세포의 사멸을 유도하고 이에 대한 성장인자의 효과를 정량적으로 분석하였다. 결과: 30M의 할로페리돌을 배양한 일차 대뇌 신경세포에 24시간 처리하면 광범위한 신경세포의 사멸이 유도된다. 이때 신경세포는 세포의 응축, DNA의 단편화와 응집, 세포막보다 핵막의 이른 붕괴 등 고사 형태의 신경세포 사멸 특성을 보인다. 할로페리돌과 더불어 신경성장인자인 BDNF, NT 4/5 및 인슐린 유사 성장 인자인 IGF-Ⅰ 혹은 IGF-Ⅱ를 동시에 처리할 경우 용량 의존적으로 신경세포의 사멸을 억제한다. 결론: 본 연구의 결과는 할로페리돌에 의한 신경독성이 성장인다들에 의해 억제될 수 있음을 보여주는 것이며, 장기적인 항정신병약물의 사용 후에 발생하는 부작용인 지발성 운동장애의 한가지 원인 가설로 대두되고 있는 항정신병 약물에 의한 신경독성을 신경성장인자에 의해 억제할 수 있을 가능성을 제시하는 것이다. 중심단어:할로페리돌·고사·세포사멸·고사·BDNF·NT-4/5·IGF-Ⅰ·IGF-Ⅱ. Objectives: We examined the effects of neurotrophins and insulin-like growth factors on cell death induced by haloperidol, a typical anti-psychotic agent. Method: Neocortices from 14- or 5-daysold fetal mice for neuron-glia co-cultures were used for this experiment. Result: Twenty-four hours treatment of mouse cortical cell cultures with 30 M haloperidol-induced wide spread neuronal apoptosis characterized by cell body shrinkage, DNA frag-mentation and condensation. Concurrent treatment with growth factors, BDNF, NT4/5, IGF-Ⅰand IGF-Ⅱ, protect the neurons from the haloperidol-induced neuronal apoptosis(HINA) in a dose dependent manner(10-100ng/ml). Conclusion: The present study suggests the possibility that haloperidol toxicity can be hampered with growth factors. Further study about the mechanism underlying the protective capacity of the growth factors on HINA may lead to the development of the new protective strategy for tardive dyskinesia. KEY WORDS:Haloperidol·Apoptosis·Neuronal death·BDNF·NT4/5·IGF-Ⅰ·IGF-Ⅱ.