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신재형 ( Jaehyeong Shin ),신재범 ( Jaebeom Shin ),엄건호 ( Gunho Eom ),이경준 ( Kyeong-jun Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5
This study was conducted to determine the supplemental effects of two insect meals, mealworm (MW) and black soldier fly (BSF), with high or low lipid levels in diets, on Pacific white shrimp Litopenaeus vannamei. Sardine and tuna by-product meals were used as the fish meal source in a control (Con) diet. The fish meals were replaced with MW, defatted MW (deMW), BSF or defatted BSF (deBSF), respectively. The shrimp (body weight: 0.47 g) were stocked into 20 acryl tanks (215 L) and fed the diets six times a day. After 45 days of the feeding trial, the shrimp that were fed insect meals had significantly higher phenoloxidase and superoxide dismutase activities than the shrimp fed Con diet. The gene expressions of prophenoloxidase, crustin and penaeidine-3c in shrimp hepatopancrease were also higher in shrimp that were fed the insect diets, regardless of defatting than those in shirmp that were fed Con diet. The survival against Vibrio parahaemolyticus was higher in shrimp that were fed the diets containing defatted insect meals than in shrimp that were fed Con diet. These results indicate that MW and BSF, regardless of lipid levels, could be good protein sources for the enhancement of innate immunity and anti-oxidant capacity of the shrimp.
안재범(Jaebeom Ahn),신효성(Hyoseong Shin),이정언(Jeongeon Lee),김성동(Sungdong Kim) 유공압건설기계학회 2019 유공압건설기계학회 학술대회논문집 Vol.2019 No.6
This study is designed to solve the problem of a person suffering from paralysis of the whole body due to muscle diseases such as amyotrophic lateral sclerosis. Full-body paralysis patients cannot move or speak because of muscular dystrophy and weakness. Eye movement is considered in the study to communicate with other people because they could move their eyes. Arduino is used to implement the eye-motion communication system. The system is composed with eye-keyboard, glasses with QTR sensor and laser pointer-receiver which are attached to eye glasses. When the laser pointer is aiming some letter on a eye-keybard, the QTR sensor is used to recognize the movement of the eyeball so that the letter is inputted and the word is printed on the monitor. Along with that, eye-motion is displayed on the Neopixel LED matrix so that we can communicate from a long distance. The test results showed that it took an average of 8.2 seconds to enter a word of 4 characters, and an average of 43.6 seconds to make a sentence of 4 words for a novice. This design shows the actual implementation potential of the eye-keyboard.
흰다리새우(Litopenaeus vannamei) 사료 내 괭생이모자반(Sargassum horneri) 분말의 이용가능성 평가
엄건호 ( Gunho Eom ),신재범 ( Jaebeom Shin ),임세진 ( Se-jin Lim ),이규태 ( Kyu-tae Lee ),한영석 ( Young-seok Han ),이경준 ( Kyeong-jun Lee ) 한국수산과학회(구 한국수산학회) 2020 한국수산과학회지 Vol.53 No.6
This study was conducted to evaluate dietary supplementation of Sargassum horneri (SH) meal on growth performance, feed utilization, innate immunity, antioxidant capacity and disease resistance of Pacific white shrimp. The diets were added with graded levels of SH meal by 0, 0.5, 1, 2, 4 and 8% (designated as Con, SH0.5, SH1, SH2, SH4 and SH8, respectively). Quadruplicate groups of shrimp were hand-fed with one of the diets five times daily for 39 days. The innate immunity and antioxidant capacity of shrimp were significantly improved by the dietary SH supplementation. The cumulative survival of shrimp exposed to Vibrio parahaemolyticus in a challenge was higher in shrimp group fed SH diets, except for SH8, than that of shrimp fed the control diet. Growth performance and feed utilization of the shrimp were significantly decreased with the dietary SH inclusions except for 0.5%. Results indicated that dietary SH meal could be used as a functional supplement for improvements in innate immunity, antioxidant capacity and disease resistance in Pacific white shrimp. The suggested dietary inclusion level of dried SH is approximately 0.5% for the shrimp.
Au nanozyme-driven antioxidation for preventing frailty
Kim, Jeonghyo,Oh, Sangjin,Shin, Yong Cheol,Wang, Caifeng,Kang, Moon Sung,Lee, Jong Ho,Yun, Woobin,Cho, Jin Ah,Hwang, Dae Youn,Han, Dong-Wook,Lee, Jaebeom Elsevier 2020 Colloids and surfaces Biointerfaces Vol.189 No.-
<P><B>Abstract</B></P> <P>From senescence and frailty that may result from various biological, mechanical, nutritional, and metabolic processes, the human body has its own antioxidant defense enzymes to remove by-products of oxygen metabolism, and if unregulated, can cause several types of cell damage. Herein, an antioxidant, artificial nanoscale enzyme, called nanozyme (NZs), is introduced that is composed of Au nanoparticles (NPs) synthesized with a mixture of two representative phytochemicals, namely, gallic acid (GA) and isoflavone (IF), referred to as GI-Au NZs. Their unique antioxidant and anti-aging effects are monitored using Cell Counting Kit-8 and senescence-associated β-galactosidase assays on neonatal human dermal fibroblasts (nHDFs). Furthermore, alterations in epidermal thickness and SOD activity are measured under ultraviolet light to investigate the effects of the topical application of NZs on the histological structure and antioxidant activity in hairless mice skin. Then, hepatotoxicity and nephrotoxicity in the hairless mice are monitored. It is concluded that the NZs can effectively prevent serial passage-induced senescence in nHDFs, as well as oxidative stress in mice skin, suggesting a range of strategies to further develop novel therapeutics for acute frailty.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>In vitro</I> and <I>in vivo</I> antioxidant effects of Phyto-Au NZs are shown to deter frailty. </LI> <LI> Phyto-Au NZs showed enhanced catalytic activity. </LI> <LI> Cytoprotective effects against serial passage and H<SUB>2</SUB>O<SUB>2</SUB>-induced senescence were shown. </LI> <LI> Phyto-Au NZs caused a decrease in epidermal thickness and an increase in SOD activity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>