Effects of Apolipoprotein A-I on Apoptosis and Cytokine Production in Human Neutrophils

Hyung-Kon Kang(강형곤),Jae-Hyung Choi(최재형),Jae-Taeck Huh(허재택) 한국생명과학회 2011 생명과학회지 Vol.21 No.1

아포지방단백 A-I (apoA-I)은 항염증 및 항산화 작용을 가지고 있는 것으로 알려져 있다. 본 논문에서는 인간혈액의 호중구를 이용하여 in vitro 상태에서 세포사멸과 시토카인의 분비 과정에 미치는 apoA-I의 영향을 조사하였다. 인간 호중구에 apoA-I을 처리한 결과 세포사멸의 정도가 감소되었다. 또한, apoA-I을 함유하는 고밀도 지방단백(HDL)에 의하여도 세포사멸은 현저히 감소되었다. HDL이 결합하는 수용체인 scavenger 수용체 B-1에 대한 항체에 의하여도 세포사멸은 억제되었다. ApoA-I을 20 시간 처리한 세포의 배양액내 인터루킨-8, 인터페론-유도 단백질 10(IP-10) 및 종양괴사인자-α의 분비가 현저히 증가하였다. 호중구에 인터페론 감마와 apoA-I을 동시 처리하였을 때 시토카인 중에서 IP-10과 종양괴사인자-α의 분비가 단독으로 처리한 경우에 비해 현저히 증가되었다. 이러한 결과들은 인간 혈액 호중구에 apoA-I을 처리한 경우 호중구가 활성화되고 호중구의 세포사멸이 지연된다는 것을 제시하는 것으로 apoA-I이 호중구에 대하여 염증성 인자로 작용할 것으로 추정된다. Apolipoprotein A-I (apoA-I) has anti-inflammatory and anti-oxidative properties. This study was designed to investigate whether apoA-I affects apoptosis and cytokine production of human blood neutrophils in an in vitro culture system. Spontaneous apoptosis of neutrophils was significantly delayed by apoA-I. In addition, high density lipoprotein containing apoA-I also delayed apoptosis of neutrophils. Apoptosis of neutrophils was inhibited by anti-scavenger receptor type B-I antibodies. The amounts of interleukin-8, interferon (IFN)-inducible protein 10 (IP-10), and tumor necrosis factor-α (TNF-α) in the supernatants of cultured neutrophils treated with apoA-I were significantly increased. Combined treatment of neutrophils with IFN-γ and apoA-I produced higher amounts of IP-10 and TNF-α than did treatment with IFN-γ or apoA-I alone. The present study reveals that apoA-I activates neutrophils to produce cytokines and delays spontaneous apoptosis of neutrophils. These findings suggest that apoA-I, although a well-known negative acute-phase protein, has a pro-inflammatory effect in neutrophils.

흉강내 수막류 1 예

허재택,이영우 대한신경외과학회 1982 Journal of Korean neurosurgical society Vol.11 No.4

흉강내 수막류는 매우 희귀한 질병이며 이제까지 보고도 적다. 본 교실에서 46세의 여자환자에서 가슴과 상배부에 다발성 지방종을 동반한 흉강내 수막류 1예를 경험하였기에 문헌고찰과 함께 보고한다. An intrathoracic meningocele is a saccular protrusion of the meninges through a normal or enlarged intervertebral foramen or bony defect of the thoracic vertebrae. It extends aneteriorly between the ribs and into the thoracic cavity, displacing the pleura and lung and lying in the paravertebral gutter if of sufficient size. Meningocele, single or multiple, are prone to occur in cases of von Reckling-hausen's neurofibromatosis, often in association with kyphoscoliosis. In this case, we found a case of intrathoracic meningocele associated with multiple lipoma of variable sizes in the back and hyperpigmentation in the anterior chest and upper back region but no evidence of neurofibromatosis. Because of rarity of this disease entity, we report a case of intrathoracic meningocele with a review of literature.

만성 척수손상 환자에 대한 대망전위수술 : 예비보고 Preliminary Report

허재택,김형동,최휴진,김기욱 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.7

The omentum has been used over the years for a variety of clinical problems. Recently it has shown that placing the omemtum on the brain and spinal cord can lead to an extensive development of vascular connections at the omental/CNS interface. Success with omental transposition to the human brain has led to increasing intreast in placing the omentum onto the human spinal cord. One paraplegic patient was chosen to enter into a feasibility study to see if omental transposition to their spinal cord might result in clinical benefit. The length of time from injury was about 21 months respectively. This patient had little, if any, motor and sensory function below umbilicus level. The operation required surgical lengthening of the pedicled omentum followed by its placement into a subcutaneous tunnel created backward along the lateral chest wall up to T-10, 11 level. An extensive thoracic laminectomy was then performed followed by a wide opening of the dura. In our case the cord showed segmental shrinkage of the spinal cord consistent with previous trauma. The omentum was laid directly onto the underlying spinal cord. Our observation for five months has shown that placement of the omentum onto the chronically injured spinal cord allowed for subsequent improvement in neuroelectrical activity, as manifested by reproducible somatosensory evoked potentials, and, more importantly, in moter function. It is considered that placing the omentum directly upon the brain or spinal cord may have the effect of either improving local vascular perfusion or, possibly, exerting some biochemicals(neurotransmitter), or as yet unknown, influence. But further observation is warranted to determine whether this improvement will be continued and will be observed in another cases.

고양이에서 척수 외상으로 인한 심부정맥에 관한 연구

허재택,이영우 대한신경외과학회 1982 Journal of Korean neurosurgical society Vol.11 No.2

This study was undertaken to determine the cardiovascular response to the spinal cord trauma in cats and to determine the autonomic mechanisms involved. The electrocardiogram and arterial blood pressure were recorded in anesthetized cats after the spinal cord trauma of 500 gm-㎝ at T4-T6 levels. 1. Acute spinal cord trauma caused a wide variety of severe cardiac arrhythmias and acute hypertension. 2. The increase of blood pressure could be prevented by intravenous phenoxybenzamine, an alpha adrenergic blocking agent. The pressor response was mediated by the alpha adrenergic receptor sites of the peripheral nervous system. 3. Tachycardia was occurred by the administration of atropine and bilateral vagotomy before trauma without affecting pressor response. 4. The arrhythmias could be eliminated by the combined administration of propranolol and atropine, but not prevented by the administration of either propranolol or atropine without affecting pressor response. These results suggest that the arrhythmias might be a response of hyperactivity of both sympathetic and parasympathetic divisions of the autonomic nervous system.

잠수병의 방사선학적 소견 및 치료에 대한 연구

허재택 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : The aim of this study are to detect the radiological findings of decompression sickness that could elucidate the pathogenesis of decompression sickness and to evaluate the results of management of decompression sickness with hyperbaric oxygen therapy. Methods : Forty nine patients(42 men and 7 women; 23-51, mean 32 years) were referred to the Hyperbaric Oxygen Therapy Center of Dong-A University Hospital for diving related injuries. All patients were studied neurologically and radiologically. Forty patients(81.6%) were type 1 decompression sickness who complained pains in various sites. Eight patients(16.3%) were type 2 decompression sickness with spinal cord involvement and underwent MR examination of the spinal cord. One patient(2.0%) was type 2 decompression sickness with chest involvement. Two patients(4.1%) among nine patients of type 2 decompression sickness were combined with type 3 decompression sickness who was suspected of cerebral embolization. All patients of type 1 were treated with therapeutic hyperbaric recompressions of US Navy treatment table 5 and auxiliary therapy. Six patients of type 2 were treated with US Navy treatment table 6 and two patients of type 3 were treated with US Navy treatment table 6A. Results : Magnetic Resonance Image(MRI) demonstrated patchy areas of increased signal intensity in the spinal cord on both T2-weighted and fast SE T2-weighted images in five patients with symptoms of spinal cord injury, corresponding to an area of the cord believed to be clinically involved. Chest X-ray and spiral computed tomogrphy(CT)scan demonstrated patchy area in right middle lobe of lung in type 2 decopmression sickness of chest involvement and disappeared two weeks later with hyperbaric oxygen therapy. All patients of type Ⅰ decompression sickness were improved with hyperbaric oxygen therapy and auxiliary therapy. Motor weakness of extrimities were improved immediately during hyperbaric therapy within hyperbaric chamber in six patients among eight patients of type 2 decompression sickness with spinal cord involvementbut. Two patients who were not improved during first time of hyperbaric oxygen therapy were remained neurological deficit 6 months later. One patient of type 2 decompression sickness with chest involvement were also improved with hyperbaric oxygen therapy and auxiliary therapy. Two patients of type 3 decompression sickness combined with hype 2 were also improved neurologically after hyperbaric oxygen therapy with auxiliary therapy. Conclusion : MRI has proved to be reliable in the detection of spinal cord involvement due to decompression sickness that were previously undetectable by other neuroradiological investigations(such as myelography, CT, angiography, isotopic tests) and its pathologic findings might be corresponded to ischemic injury of spinal cord. Patch area which was demonstrated by chest spiral CT and chest P-A of type 2 decompression sickness might be related to hemorrhage of lung parenchyme due to alveolar rupture which was resolved two weeks later. Therapeutic hyperbaric recompression is very effective in the management of decompresion sickness.

가토 대뇌피질 Synaptosome에서 Na^(+)-dependent ^(14)C-GABA이동에 미치는 Lidocaine의 영향

허재택 부산의대 1988 부산의대잡지 - 부산대학교 의과대학 Vol.28 No.2

저체온 및 순환정지를 이용하여 모야모야 병이 동반된 뇌기저 동맥 첨부의 복합성 뇌동맥류 수술

조철민,허재택,하상수,우종수,이수일 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.5

Giant and complex aneurysm of the posterior circulation can pose several technical challenges with high operative morbidity. Recent advances in cardiac surgery have raised interest in the technique of deep hypothermic circulatory arrest for the management of giant and complex intracranial aneurysms of posterior circulation. The criteria for selecting patients for this procedure can be based on a preoperative analysis of available studies that suggests high risk with standard intervention. Using the technique of deep hypothermic circulatory arrest, we have successfully operated on a case of complex basilar tip aneurysm with MoyaMoya disease resulting in no significant neurological complications. We therefore suggest that patients with giant and complex intracranial aneurysms of posterior circulation might benefit from the use of deep hypothermic circulatory arrest technique.

소뇌충부에 발생한 해면상혈관종 1례

박동준,허재택,장병조,이영우 대한신경외과학회 1987 Journal of Korean neurosurgical society Vol.16 No.3

A case of cavernous angioma of the cerebellar vermis is described. Cavernous angioma is rare vascular malformation of the central nervous system and the cerebellar vermis is very unusual site. Exact preoperative diagnosis of this lesion is difficult because cavernous angioma is angiographically silent and generally accepted to be very rare. The relevant literature is reviewed.

유아의 결합조직형성 대뇌 성상세포종

김경범,허재택,송영진,허해경,나서희,정진숙,최순섭 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.12

뇌동맥류가 파열된 산모에 제왕절개술과 뇌동맥류 클리핑을 동시에 시행한 마취관리

이수일,황기백,이정유,허재택,배승환 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.32 No.3

Aneurysmal subarachnoid hemorrhage in a pregnant woman is a rare catastrophic situation that places both the mother and fetus at high risk. When this situation arises, numerous relevant issues must be individualized in the effort to reduce hazards threatening both the mother and fetus. A 29 year old woman who was 39 weeks pregnant presented with a subarachnoid hemorrhage, secondary to a ruptured left ophthalmic artery aneurysm. Following initial full recovery, the simultaneous sequence of cesarean section followed by aneurysmal clipping was electively underwent. Successful maternal and perinatal outcome was achieved. The anesthetic management of the case is described and discussed. (Korean J Anesthesiol 1997; 32: 463∼466)