말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

각종 소화성질환에서의 Campylobacter Pylori의 검출에 관하여

허정욱,강영우,박준호,박승국,하경임,김재룡,안성훈,박선우 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

Peptic disorders are among the most common maladies in korea as the world. In most cases their etiologies not be established and their pathophysioolgy remain obscure. Although the persence of fastric bacteria has been logn established, recently campylobacter pylori seems to be etiologic agent of peptic disorders. Campylobacter pylori is visualized in most cases of peptic disorders and not visualized after treatment of bctericidal agents, bismuth compound or metronidazole. We had a following results for identification of campylocacter pylori in stomach and duodenal mcuosa via endoscipc biopsy. 1. We studied 54 patients who had dyspeptic symptoms, 29 males. 2. Most patients are in 5th decades, next in 3rd and 6th in order. 3. Ptients were composed of 27 peptic ulcer, 22 gastitis and 5 gastric carcinoma. 4. Campylobacter pylori was identified in 69% of total patients, peptic ulcer 63%, gastritis 77%, stomach carcinoma leastly.

간세포암의 고주파 열치료 후 천자침 경로를 통한 전이 1례

조영완,박석주,진한영,김준영,이재익,강명주,박정하,윤정희,박성재,지삼룡,이연재,이상혁,설상영 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiofrequency ablation(RFA), as a form of minimally invasive therapy for hepatocellular carcinoma, has become an important treatment modality. Because of limitation of surgery, RFA has become standard therapy for hepatocellular carcinoma in some situations. But there are some complications of RFA such as bleeding, infection, hematoma, adjacent organ thermal damage including intestinal perforation, needle track seeding, and so on. There are few reports in the literature that systematically evaluate the incidence of needle tract seeding and its associated risk factors. And only 2 cases of needle tract seeding of hepatocellular carcinoma after RFA were reported in Korea. We report a case of needle tract seeding of hepatocellular carcinoma after RFA.

단섬유강화 폴리프로필렌 복합재의 환경온도변화에 따른 파괴강도특성에 관한 연구

하재룡(Jae-Ryong Ha) 산업기술교육훈련학회 2011 산업기술연구논문지 (JITR) Vol.16 No.4

In this paper, the fracture mechanism and the properties of microscopic structure of glass fiber reinforced plastics produced by shouting machine was investigated by universal testing instrument and SEM in varied environment temperature. 1) The tensile strength decreased with an increase of environment temperature. The above trend was more dominant in the parent specimen than in the weld specimen. 2) When environment temperature changing, the changing of tensile strength in parent is higher than that of weld part, because it is much influenced by fiber compared to matrix. The strength decrease in welding point is much influenced by fiber direction than composition condition as V-notch in weld line. 3) The fracture surface observation by SEM at low temperature(-30℃) showed fiber breaks in the matrix. But at high temperature(80℃), some of fibers were ruptured but some were delaminate with plastic deformation in the matrix. The tensile strength was higher at low temperature than at high temperature, which was believed to be due to the difference in binding force between matrix and fiber.

논문 : 암반의 불균질성을 고려한 불포화대 지하수 유동 평가

하재철 ( Jae Chul Ha ),이정환 ( Jeong Hwan Lee ),정재열 ( Jae Yeol Cheong ),정해룡 ( Hae Ryong Jung ) 대한지질공학회 2016 지질공학 Vol.26 No.1

본 연구에서는 단열을 포함하는 불포화 암반에서의 지하수 유동 흐름을 예측하기 위한 2차원 수치 모델링을 수행하였다. 특히, 불연속 단열망 모델링(Dscrete Fractured Network, DFN)을 통하여 도출된 투수계수의 분포를 나타내는 k-field를 모델링 입력변수로 적용하였다. 투수계수 차이는 불포화대에서 지하수의 이동속도 차이를 야기시키는 중요한 인자로 적용되었다. 연구지역의 지표 토양층으로부터 지하 대수층까지의 불포화대 깊이를 적용하여 지하수 유동 모델링의 초기조건을 실제와 유사하게 설정하였다. 강우의 지표 침투율은 인공구조물과 자연 토양층의 침투율 차이를 적용하여 실제 불포화 암반을 거쳐서 포화대까지 지하수의 이동흐름을 해석하고 시각화하였다. 특히, 오염물질의 이동 시작점이 될 수 있는 인공구조물의 하부에 모니터링 지점을 설정하여 실제 오염물질원이 지하수에 용해되어 불포화대를 이동할 때의 경로를 예측 하였으며 중력방향인 직하부로 이동하는 것을 확인되었다. We present the results of two-dimensional numerical simulations predicting the flow of groundwater in a fractured unsaturated zone. We applied the k-field distribution of permeability derived from discrete fracture network (DFN) modeling as the hydraulic properties of a model domain. To model an unsaturated zone, we set the depth from the ground surface to the underground aquifer. The rate of water infiltration into the unsaturated zone was divided into two parts, an artificial structure surface and unsaturated soil zone. The movement of groundwater through the unsaturated zone was simulated with particular emphasis on contaminant transport. It was clearly observed that the contaminants dissolved in groundwater transported vertically from the ground surface to the saturated zone.

Seroepidemiological Survey of Zoonotic Diseases in Small Mammals with PCR Detection of Orientia tsutsugamushi in Chiggers, Gwangju, Korea

Jung Wook Park,Jae Keun Chung,Sun Hee Kim,Sun Ju Cho,Yi Deun Ha,So Hyang Jung,Hye Jung Park,Hyun Jae Song,Jung Yoon Lee,Dong Min Kim,Jah Pyus,Dong Ryong Ha,Eun Sun Kim,Jae Il Lee 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.3

Association between Fasting Blood Glucose Levels and Arterial Stiffness in Healthy Korean Men and Women

( Jae Woong Sull ),( Dae Ryong Kang ),( Sun Ha Jee ) 한국보건정보통계학회(구 한국보건통계학회) 2014 한국보건정보통계학회지 Vol.39 No.2

Background: Increased fasting blood glucose (FBG) level and obesity can be associated with the progression of arterial stiffness, which is an early marker of atherosclerosis. Measuring pulse wave velocity (PWV) is a non-invasive method to evaluate vessel wallstiffness. Objective: The objective was to determine the association of FBG levels and obesity with peripheral PWV considering insulin resistance. Methods: Across-sectional study was conducted in 2006. Waist circumference (WC), body mass index (BMI), and PWV were measured in 3,390 healthy Korean men and women. Multiple linear regression models were used to assess the association between FBG levels and peripheral PWV. The population was classi- fied into two groups according to median insulin resistance values (homeostasis model assessment of insulin resistance [HOMA-IR]). Results: Peripheral PWV was significantly associated with BMI and systolic blood pressure in men and women, respectively, after adjusting for age, lipid levels, and HOMA-IR (Table 3). WCwas associated with peripheral PWV only in women ( p=0.006). The relationship between peripheral PWV and FBG in women was significant in subjects with HOMA-IR < 0.725 (p= 0.015). Conclusions: These results suggest that FBG levels and waist circumference are associated with peripheral PWV in Korean women. Further studies should be performed in other populations to confirm the association between FBG levels and PWV considering insulin resistance.

Comparison of polymer-based temporary crown and fixed partial denture materials by diametral tensile strength

Ha, Seung-Ryong,Yang, Jae-Ho,Lee, Jai-Bong,Han, Jung-Suk,Kim, Sung-Hun 대한치과보철학회 2010 The Journal of Advanced Prosthodontics Vol.2 No.1

PURPOSE. The purpose of this study was to investigate the diametral tensile strength of polymer-based temporary crown and fixed partial denture (FPD) materials, and the change of the diametral tensile strength with time. MATERIAL AND METHODS. One monomethacrylate-based temporary crown and FPD material (Trim) and three dimethacrylate-based ones (Protemp 3 Garant, Temphase, Luxtemp) were investigated. 20 specimens (Ø 4 mm × 6 mm) were fabricated and randomly divided into two groups (Group Ⅰ: Immediately, Group Ⅱ: 1 hour) according to the measurement time after completion of mixing. Universal Testing Machine was used to load the specimens at a cross-head speed of 0.5 mm/min. The data were analyzed using one-way ANOVA, the multiple comparison Scheffe′test and independent sample t test (α= 0.05). RESULTS. Trim showed severe permanent deformation without an obvious fracture during loading at both times. There were statistically significant differences among the dimethacrylate-based materials. The dimethacrylate-based materials presented an increase in strength from 5 minutes to 1 hour and were as follows: Protemp 3 Garant (23.16 - 37.6 MPa), Temphase (22.27 - 28.08 MPa), Luxatemp (14.46 - 20.59 MPa). Protemp 3 Garant showed the highest value. CONCLUSION. The dimethacrylate-based temporary materials tested were stronger in diametral tensile strength than the monomethacrylate-based one. The diametral tensile strength of the materials investigated increased with time.

Water-resistant PEDOT:PSS hole transport layers by incorporating a photo-crosslinking agent for high-performance perovskite and polymer solar cells

Ha, Su Ryong,Park, Sumin,Oh, Jae Taek,Kim, Do Hui,Cho, Shinuk,Bae, Sung Yong,Kang, Dong-Won,Kim, Jong-Man,Choi, Hyosung The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.27

<P>We demonstrated a water-resistant PEDOT:PSS HTL by incorporating a photo-crosslinking agent into a PEDOT:PSS film. A crosslinking system was successfully formed inside the PEDOT:PSS film by simple and fast photo-polymerization of PCDSA monomers. Combination of the crosslinking system and MeOH surface treatment simultaneously improved the device efficiency and stability of both perovskite and polymer solar cells. The crosslinking system inside PEDOT:PSS changed its intrinsic water-soluble characteristic into a water-resistant property, thus preventing water penetration into the PEDOT:PSS film. In addition, MeOH treatment improved the surface conductivity and reduced the surface roughness of the PEDOT:PSS film by removing surface residues of PDAs and insulating PSS parts.</P>

Extra LD6 Isoenzyme Band 1례

하경임,전동석,김재룡,현정애,전종찬 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.1

Normal human serum and tissue, except testis, show five lactate dehydrogenase isoenzymes on electrophoresis. An unusual band on the cathodic side of LD?? isoezyme has been found on lcatate dehydrogenase eletrophoresis of serum, called LD??, and the presence of which signifies a poor prognosis for the patients. The autors experience one case of LD?? Extraband in 61-year-old female patient suffered from rheumatic heart disease combined with aortic stenosis and regurgitation, congestive heart failure and pulmonary edema. Cardiac arrest was noted within several hours after exhibition of LD?? Extraband and she did not recover from her illness, so we report with brief review of literatures.