일산화탄소 및 고압산소의 폭로가 흰쥐의 제2형 폐포세포와 사구체에 미치는 영향에 관한 연구

백두진,정호삼,이계훈,황세진,전영호,전영희 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.2

The present study was performed to investigate the ultrastructural changes of the type Ⅱ pneumocyte in the lung and that of the glomerulus in the kidney after carbon monoxide(CO) or hyperbaric oxygen (HBO) exposure. The male Sprague-Dawley rats, weighing about 200gm, were used as experimental animals. The animals were divided into CO exposure group, air exposure after CO exposure group, HBO exposure group and HBO exposure after CO exposure group. After single CO and HBO exposure (3790ppm), the specimen of lung and kidney were obtained. According to routine method, EM preparations were made and observed with electron microscope. The results obtained were as follows ; 1. Slight disruption of the mitochondrial membrane was observed in alveolar type Ⅱ pneumocyte, after single CO exposure. 2. In all experimental groups except CO exposure group, decrease in number and atropy of rough endoplasmic reticulum, disruption of double membrane and crista of mitochondria and vacuolar degeneration of lamellar bodies in alveolar type Ⅱ pneumocytes were observed. 3. In all experimental groups, fusion of the pedicles of podocytes, and obliterations of the endothelial fenestrations were observed. These results suggested that CO and HBO exposure may induced the ultrastructural damages in the alveolar type Ⅱ pneumocyte and the glomerulus.

Daunomycin이 Mouse 간장의 Alkaline Phosphatase 활성에 미치는 영향

백두진,김훈기,정호삼 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Daunomycin is a member of the anthracycline class of antitumor antibiotics. And it was isolated from cultures of Streptomyces peucetius in 1963. Cemically, it was found to consist of a pigmented aglycone (daunomycinone) bound to an aminosugar, daunosamine. The biochemical mode of cytotoxic action of daunomycin results from its binding to DNA possibly by intercalation between the base pairs, thereby inhibiting the DNA polymerases. The author has investigated the effect of daunomycin on the mouse liver histochemically observing the change in the activity of alkaline phosphatase. The animals treated with 16 mg per kg of daunomycin wee sacrified at 6, 12, 24 and 36 hours after drug administration. The animals of control group were administered only water for injection. The liver specimens were fixed in 10% neutral formalin at 4℃ and sectioned at 16? In thickness in a frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method. The results were as follows. 1. The activity of alkaline phosphatase was moderate positive at the periportal and intermediate zones and trace positive at the centrilobular zone of the hepatic lobule in 6 hours daunomycin treated group. Ad the time goes by, the activity of alkaline phosphatase was decreased in the hepatic lobule. At 24 hours after administraton of daunomycin, negative reaction at the centrilobular zone, trace positive at the intermediate zone and weak positive at the periportal zone of the hepatic lobule were seen. Consequently, it is suggested that daunomycin decreases the activity of alkaline phoshatase in the liver, due probably to its cytotoxic effect on the hepatocyte.

Vinblastine Sulfate가 Mouse간장의 인산염 분해효소 활성에 미치는 영향

백태경,박희철,정호삼 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Vinblastine sulfate isolated from the vinca has been widely used as an anticancer drug. Vinblastine sulfate inhibits the formation of microtubules which are related to cytoskeleton, transport of molecules and cellular polarity. As a result, it inhibits the mitosis and brings to cellular necrosis. The author undertook the present study to investigate the effect of binblastine sulfate on phosphatase activity in the mouse liver. Albino mice, DDY strain, weighing 2gm were used as experimental animals. The experimental animals were sacrificed at 6, 12, 24, 36 and 48 hours after administration of 12 mg/kg of of vinblastine sulfate. The activity of alkaline phosphatase was observed by Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein-Meisel's method for histochemical study. The results obtained were as follows: 1. The activity of alkaline phosphatase was slightly increased in the central zone and decreased in the intermediate and periportal zone of the hepatic lobule of the 24 and 36 hours vinblastine sulfate treated groups. In 48 hour-vinblastine sulfate treated group, the activity of alkaline phosphatase was recovered to normal. 2. The activity of adenosine triphosphatase was slightly increased in the hepatic lobule of the 12 and 24 hours vinblastine sulfate treated groups but decreased in the 36 and 48 hour vinbalstine sulfate treated groups. Consequently, it is suggested that vinblastine sulfate cause to decrease the activity of alkaline phosphatase and adenosine phosphatase in mouse liver.

보강판의 트리핑에 관한 연구

백점기,이원호 釜山大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.52 No.-

본 연구의 목적은 보강재의 트리핑의 특성을 수치적으로 조사하고 보강재웨브의 국부좌굴강도를 예측하기 위한 설계식의 정도를 검증하는데 있다. 또한 비선형 유한요소법을 이용하여 보강재의 트리핑과 보강재간 패널의 국부좌굴의 상관효과 및 판과 보강재의 접합선에서 탄소성 회전구속의 영향등을 조사하는 것이다. 기존의 유한요소법은 구조물의 비선형해석을 수행함에 있어서 상당한 컴퓨터계산을 요하지만, 본 연구에서는 보강판의 비선형거동을 보다 효율적으로 계산하기 위하여 간이화된 비선형유한요소법을 적용하였다. 압축력을 받는 flat-bar형 보강재가 붙은 보강판에 대하여 탄소성대변형 유한요소해석을 판과 보강재웨브의 치수를 변화시켜 가면서 시리즈해석을 수행하여, 그 결과를 바탕으로 flat-bar형 보강재의 트리핑에 대한 기본적인 조사를 수행하였다. 또한, 본 연구에서는 보강재웨브의 임계 좌굴강도를 예측하기 위한 2개의 설계식 즉, IACS설계식과 Paik등이 개발한 간이식을 이용한 이론해와 유한요소해석 결과를 비교함으로써 그 정도를 검증하였다. The twin aims of the present study are to numerically investigate the characteristics of tripping failure of flat-bar stiffened panels subject to uniaxial compressive loads and also to study the accuracy of two available design formulations. A special purpose nonlinear finite element method capable of efficiently analyzing the elasto-plastic large deformation behavior of stiffened panels is developed and used in the study. A benefit of the application of the nonlinear finite element method is that it makes possible a rigorous accounting of the interacting effects of stiffener tripping and plating collapse and also the inclusion of the influence of elasto-plastic rotational restraints at the plate-stiffener intersection prior to and during failure. A parametric series of elasto-plastic large deformation analyses for stiffened panels with flat-bar type of stiffeners under uniaxial compressive loads are carried out varying member proportions and structural parameters. Based on the computed results, a basic investigation of tripping behavior of flat-bars is made, and the accuracy of design formulations is studied.

Phase-Type 분포를 이용한 보증서비스 비용 분석

김호균,백천현,조형수 한국경영과학회 2000 韓國經營科學會誌 Vol.25 No.3

We consider the question of servicing warranties for repairable items. During the warranty period, each time an item fails the manufacturer has the obligation to restore the item to operational condition either by repairing the item or by replacing it by a new item. For products with phase-type lifetime distributions where the phases represent the condition of the item. We develop algorithms to determine the expected cost of servicing a warranty and use it in making the repair/replacement decision. Illustrative numerical examples are presented.

VDT 업무가 근골격계장애에 미치는 영향

권호장,하미나,김돈규,백남종,조수헌,한태륜 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

As use of the visual display terminal(VDT) is becoming more generalized as a result of office automation, the so-called 'VDT syndrome' which is often observed with the workers using VDT has emerged as a serious occupational health problem. However, few comparative study with control group using not only subjective symptom but also physical examination has been conducted. We have conducted a study comparing prevalence rated of musculoskeletal disorder between 113 VDT operators of a telecommunication company and the control groups of housewives and office ladies through a review of subjective symptoms as well as physical examination by physiatrist to understand the magnitude of problem and relative risk of VDT worker. The symptom rates of upper extremity disability in VDT operators were higher than those of controls especially in neck and shoulder area. Fifty-four(47.8%) of 113 VDT operators met our case definition of upper extremity disorder by subjective symptom and physical examination. Myofascial pain syndrome of neck and shoulder muscles were the most common(46.9%). The odd ratio of using VDT operation for developing myofascial pain syndrome in the neck or shoulder area were 2.52(compared to controls of office ladies) and 2.64(compared to controls of housewives). Other anatomical lesion such as elbow, hand and wrist shows slight higher prevalence rates of musculoskeletal disorder than control groups but not statistically significant.

Mitomycin이 Mouse의 肝細胞 내 Pyronin 好性顆粒에 미치는 影響

鄭鎬三,李圭植,白斗鎭 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Mitomycin, one of the mitosane antibiotics, is isolated from Streptomyces caespitosus which has anticancer effect. It is well known that mitomycin inhibites DNA synthesis in mammalian cells. The major machanism accounting for the inhibitory effect of mitomycin is interference of the template activities of the DNA by cross linking to DNA double helix. The auther has investigated the effect of mitomycin on the liver of mice, observing the distribution and disaggregation of the pyroninophilic granules in the hepatic parenchymal cells. A total of 52 healthy mice of ICR strain (the weight 20g) were divided into two groups; The control and the mitomycin treated groups. All experimental animals were given food and water ad libitum. The animals of the mitomycin treated groups were administered 4mg per kg of body weight of mitomycin diluted in the water for injection by intraperitoneal injection. The animals of the MC treated groups were sacrified at intervals of 6, 12, 24 and 36 hours after administration of mitomycin Histochemical preparations were stained with the methyl green-pyronin method for the pyroninophilic granules in the hepatic parenchymal cells. A marked reduction or disappearance of the pyroninophilic granules were observed in the entire hepatic lobule at 24 hours after administration of mitomycin. Consequently, it is suggested that mitomycin causes the loss of pyroninophilic granules in the hepatic parenchymal cells of the liver in the mitomycin treated mice.

Mitomycin이 Mouse 肝臟에 미치는 影響

정미경,백두진,정호삼,이규식 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

Mitomycin, one of the mitosane antibiotics, is a alkylating agent in common use as a anticancer drug, which is isolated from Streptomyces caespitosus culture media. Mitomycin interferes with the template activities of DNA by cross linking to the DNA double helix and inhibites nucleic acid synthesis. The authors have demonstrated the effect of mitomycin on the liver of albino mice morphologically, observing the cellular change and accumulation of fat in the hepatic arenchymal cells. A total of 52 healthy albino mice of ICR strain (the weight 20g) were divided into two groups; the control and the mitomycin treated groups. All experimental animals were given food and water ad libitum. The animals of the mitomycin treated groups were administered 4mg per kg of body weight of mitomycin diluted in the water for injection. The animals of the control groups were administered only water for injection. The animals of the mitomycin treated groups were sacrified at intervals of 6, 12, 24 and 36 hours after administration of mitomycin. Histological preparations were prepared by way of hematoxylin and eosin stain for the cellular changes and oil red O stain for fat accumlation in the mouse liver. The results are obtained as follow: 1. The cellular swelling associated with vesicular formation are observed in the hepatic parenchymal cells of the central and intermediate zones of hepatic lobule at 12 and 24 hours after administration of mitomycin. 2. Fat deposits in the hepatic parenchymal cells of the central zones of hepatic lobule are observed at 24 hours after administration of mitomycin. It is suggested that mitomyin causes cellular swelling accompanied with the vesicular formation and fat accumulation in the parenchymal cells of the liver in mice.

일산화탄소 폭로후 고압산소 재폭로시 흰쥐 신장 및 심장의 SOD 활성 변동에 관한 연구

전영희,백두진,황세진,박준숙,정호삼 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Carbon monoxide is generated from the incomplete combustion of carbon-containing materials. Carbon monoxide cause muscle necrosis leading to myoglobinuria and acute renal failure. The hyperbaric oxygen exposure is increased production of active free radicals. Free radicals which promoted peroxidation of unsaturated fatty acids may destroy essential cellular components and itself cytotoxic materials. Superoxide dismutase, one of the protective mechanisms against tissue damage, catalyzed the dismutation of superoxide anion to less toxic product. The author performs the present study to investigate on Mn-SOD and Cu, Zn-SOD immunoreactivities in kidney and heart carbon monoxide (CO) and hyperbaric oxygen (HBO) exposure. The animals were divided CO exposure group, air release after CO exposure group, HBO exposure group and, CO and HBO exposure group. The specimen were sectioned in 16㎛ with cryostate. The Mn-SOD and CuZn-SOD immunohistochemistry was performed in renal corpuscle, proximal and distal convoluted tubules of kidney and, in sarcoplasm and sarcolemma of heart muscle. The results obtained were as follows: 1. The immunoreactivity of Mn-SOD were increased in renal corpuscles and distal convoluted tubules, were similar to control group in proximal convoluted tubules. 2. The immunoreactivity of Cu, Zn-SOD were increased than that of control group in renal corpuscles and distal convoluted tubules, were same that of the control group in proximal convouted tubules. 3. The immunoreactivity of Mn-SOD were increased at all experimental group of sarcoplasms of heart muscle. 4. The immunoreactivity of Cu,Zn-SOD were increased than that of control group in sarcolemma of heart muscle. These results suggest that the immunoreactivity of SOD after CO or HBO exposure is greatly increased in kidney and heart.

Cadmium Chloride가 흰쥐 신장에 미치는 영향에 관한 조직화학적 연구

이규식,백두진,김원규,정호삼,백태경,김영학 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

중공업의 발달에 따라 공장등지에서 중금속인 cadmium을 이용한 제품의 생산이 증가함에 따라 자연계내 토양, 물, 식물 등에 함유된 cadmium 뿐 아니라 공업제품과 공업 쓰레기에 함유된 cadmium은 쉽게 인체에 폭로되어 인체에 유해한 결과를 초래하게 된다고 알려져있다. 이에 저자는 cadmium이 생체내 신장에 특히 오랜기간 축적된다는 보고와 또 반감기가 긴 중금속이란 점에 착안하여 신장의 피질과 수질내 조직학적 변화를 관찰하고자 하였다. 실험동물은 사육실에서 순종 분리고 4년간 사육한 Wistar계 흰쥐를 사용하였다. 흰쥐에 체중 kg당 0.6mg의 cadmium chloride를 식염수 0.2ml에 용해하여 복강내로 10주간 매주 1회씩 투여한 후 희생기켜 신장조직을 paraffin 표본으로 제작하고 Mallory-Azan 염색을 시행하여 광학현미경으로 관찰한 후 다음과 같은 결과를 얻었다. 1. Cadmium chloride 10주간 투여후 1일 경과군에서 신장의 피질과 수질에 강한 Azan 염색반응을 보였으며 신사구체의 괴사와 수질 일부에서 간질성 섬유증식 및 울혈이 관찰되었다. 2. Cadmium chloride 10주간 투여후 1주 경과군에서는 신장의 피질과 수질에 중등도의 Azan 염색반응이 관찰되었고 신사구체의 국소적 괴사가 관찰되었다. 3. Cadmium chloride 10주간 투여후 2주 경과군에서는 신장의 피질에 중등도의 Azan 염색반응이 관찰되었으며, 4주 경과군에서는 피질과 수질에 약한 Azan 염색반응만 관찰되었다. 이상과 같은 결과로 미루어 cadmium chloride의 흰쥐에 장기간 투여는 미량이라도 신장의 신사구체의 괴사, 섬유화 등이 유발되나 투여후 4주 경과시는 손상이 회복되는 것으로 사료된다. The peritoneal injections of cadmium chloride(0.6mg/kg) per 1 week for 10 weeks were made. The cadmium chloride treated rats were sacrificed on 24 hours, 1 week, 2 weeks and 4 weeks after 10 weeks administration. And each of renal tissues from cadmium treated rat kidney was stained with Mallory Azan. 1. The severe glomerular necrosis, fibrosis and congestions in 1 day group were seen in the cadmium chloride treated rats medulla. 2. The moderately positive reaction for azan stain was seen in cortex and medulla of 1 week group in the cadmium chloride treated rats medulla. 3. The weakly positive reaction for azan stain was revealed in cortex and medulla of the rat kidenys on 2 weeks and 4 weeks after cadmium chloride administration for 10 weeks. It is concluded that the renal damage of rat resulted with administration of cadmium chloride may be recovered 4 weeks after injection.