http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Imdad Ullah Khan (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
윤용석,Imdad Ullah Khan,최경욱,정태성,조광래,이수형,김완희,김대용,권오경 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.1
Cell sheets technology is being available for fracture healing. This study was performed to clarify bone healing mechanism of undifferentiated (UCS) and osteogenic (OCS) differentiated mesenchymal stromal cell (MSC) sheets in the fracture model of dogs. UCS and OCS were harvested at 10 days of culture. Transverse fractures at the radius of six beagle dogs were assigned into three groups (n = 4 in each group) i.e. UCS, OCS and control. The fractures were fixed with a 2.7 mm locking plate and six screws. Cell sheets were wrapped around the fracture site. Bones were harvested 8 weeks after operation, then scanned by micro-computed tomography (micro-CT) and analyzed histopathologically. The micro-CT revealed different aspects of bone regeneration among the groups. The percentages of external callus volume out of total bone volume in control, UCS, and OCS groups were 42.1, 13.0 and 4.9% (p\0.05) respectively. However, the percentages of limbs having connectivity of gaps were 25, 12.5 and 75% respectively. In histopathological assessments, OCS group showed well organized and mature woven bone with peripheral cartilage at the fracture site, whereas control group showed cartilage formation without bone maturation or ossification at the fracture site. Meanwhile, fracture site was only filled with fibrous connective tissue without endochondral ossification and bone formation in UCS group. It was suggested that the MSC sheets reduced the quantity of external callus, and OCS induced the primary bone healing.
-
윤용석,정태성,Muhammad Afan Shahid,Imdad Ullah Khan,김완희,권오경 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6
We assessed the efficacy of frozen-thawed gelatin-induced osteogenic cell sheet (FT-GCS) as compared to that of fresh gelatin-induced osteogenic cell sheet (F-GCS) andwith adipose-derived mesenchymal stromal cells (Ad-MSCs) used as thea control. The bB one differentiation capacity of GCS has already been studied. Based on thisOn that basis, the experiment was conducted to determinefor ease of use of GCS in the clinic. In- vitro evaluation of F-GCS showed 3– to 4 layers with an abundant extracellular matrix (ECM) formation; however, cryopreservation resulted in a reduction of FT-GCS layers to, 2– to 3 layers. Cellular viabilities of F-GCS and FT-GCS did not vary significantly. Moreover, there was no significant difference in mRNA expressions of Runx2, β-catenin, OPN, and BMP-7 between F-GCS and FT-GCS. In anthe in- vivo experiment, both legs of six dogs with transverse radial fractures were randomly assigned to one of three groups: 1) F-GCS, 2) FT-GCS, and or c3) Control. Fracture sites were wrapped with the respective cells or cell sheets and fixed with 2.7 mm locking plates with and six screws. Atfter 8 weeks after theof operations, bone samples were collected and subjected to micro- computed tomography (CT) and histopathological examination. External volumes of callus out as a portion of the total bone volume in control, F-GCS, and FT-GCS groups were 49.6%, 45.3%, and 41.9%, respectively. The histopathological assessment showed that both F-GCS and FT-GCS groups haexhibitedve significantly (Pp < 0.05) well- organized, and mature bone with peripheral cartilage at the fracture site as compared to that of the control group. ThereforeBased on our results, we inferred that the cryopreservation process did not significantly change affect the osteogenic ability of FT-GCS as comparegelatin-induced cell sheets to fresh-GCS.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
