고양이 심장에서 관상동맥 결찰과 재관류로 인한 ATPase 변화에 관한 세포화학적 연구

김은기,류임주,엄창섭,서영석 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

To understand the behaviors of Ca^(++)-ATPase and Na^(+)-K^(+) ATPase in ischemic and ischemic-reperfused heart, and to elucidate their roles in those conditions, the authors observed cat myocardium ultracytochemically. Acute ischemia was induced by ligation of the anterior interventricular branch of left coronary artery. Reperfusion was begun after 20minutes of ischemia and discontinued after 1-2 minutes when ventricular fibrillation appeared on EKG. The reaction products of Ca^(++)-ATPase were irregular In shape, and distributed mainly in myocytes in the vicinity of capillaries. In normal cardiac myocytes, they were present at plasmalemma, sarcoplasmic reticulum, transverse tubules, intercalated discs including intermediate junctions, desmosomes and gap junctions. Some were scattered along with inner mitochondrial membranes and myofibrils. After 20 minutes of ischemia. almost all structures had negligible reaction products. except some dilated sarcoplasmic reticulum beneath the plasmalemma, which retained strong reactivities. After reperfusion, the reaction products showed a tendency to be Increased. But there was hardly any reaction products in mitochondria. Patches of myofibrils with much increased enzyme activity than other myofibrils were found scattered. In addition, macrophages showed strong reactivity throughout their nuclei. Endothelial cell nuclei, however, were free of reactions in all conditions. The reaction products of Na^(+)-K^(+) ATPase were fine In nature. In normal cardiac myocytes, they were present at plasmalemma, transverse tubules, sarcoplasmic reticulum, various structures of intercalated discs, myofibrils, mitochondria, inner nuclear membranes and heterochrormatin. In ischemic heart, the reactions decreased generally and increased after reperfusion. Mitochondria in normal states had many fine reaction products scattered throughout them to the degree of masking their details. In ischemic conditions, the reaction products, decreased in number, were located contiguous to inner mitochondrial membrane. After reperfusion each reaction product was increased in size, although the amount was less than normal conditions. Gap junctions were unique structures'in that they lacked reactivity both in ischemic and ischemic-reperfused conditions. Other structures of the intercalated discs, especially desmesomes, showed increased reactions. Some myofibrils showed much strong reactions in ischemic and ischemic-reperfused groups, even though the reactivity at myofibrils as a whole was decreased. Under all conditions, the nuclei of interstitial cails and endothelial cells had their reaction products related to the nuclear membranes and heterochrormatin. The authors, based on the above observations, conclude that Ca^(++)-ATPase may have little role in ischemic and ischemic-reperfused conditions. Na^(+)-K^(+) ATPase during reperfusion, however, even depressed in their activities, may try to normalize the decreased intramitochondrial ATP contents and the increased intracellular Na^(+) concentrations resulted from ischemic conditions.

N형 전압의존성 칼슘채널 α subunit 유전자의 염색체상에서의 위치와 신경계에서의분포

박선화,김현,김형래,박수연,유임주,서영석 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.4

Expression of n-NOS mRNA is Elevated in Rolling Mouse Nagoya(RMN) Cerebelium

Im Joo Rhyu,Chang-Sub Uhm,Seung Jun Hwang,Hyun Kim,Young-Suk Suh 대한체질인류학회 1999 대한체질인류학회 학술대회 연제 초록 Vol.42 No.-

A Glance of Electron Tomography through 4th International Congress on Electron Tomography

Rhyu, Im Joo,Park, Seung Nam 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

Electron tomography (ET) is an electron microscopic technique for obtaining a 3-D image from any electron microscopy specimen and its application in biomedical science has been increased thanks to development of electron microscopy and related technologies during the last decade. There are few researches on ET in Korea during this period. Although the importance of ET has been recognized recently by many researchers, initial approach to electron tomographic research is not easy for beginners. The 4th International Congress on Electron Tomography (4 ICET) was held on Nov 5~8, 2006, at San Diego. The program dealt instrumentation, reconstruction algorithm, visualization/quantitative analysis and electron tomographic presentation of biological specimen and nano particles. I have summarized oral presentations and analyzed the posters presented on the meeting. Cryo-electron microscopic system was popular system for ET and followed conventional transmission electron microscopic system. Cultured cell line and tissue were most popular specimens analyzed and microorganisms including bacteria and virus also constituted important specimens. This analysis provides a current state of art in ET research and a guide line for practical application of ET and further research strategies.

Bergman glial cell morphology under the high voltage Electron microscope

Im Joo Rhyu 한국현미경학회 2019 Applied microscopy Vol.49 No.1

The detailed morphology of Bergam glial cell was observed in single field of view during observation of Golgi stained mouse cerebellar cortex under the high voltage electron microscopy. The 3-dimensional organization of Bergman glial cell fully demonstrated with 8-degree stereo-paired images. The morphology of Bergman glial fiber and its appendages forming microdomains connected to other glial fiber are clearly presented in this image. This image provides a valuable insight for understanding morphology of Bergman glial cell.

Electron-Microscope Contributions to Autophagy Research and the Nobel Prize in Physiology or Medicine 2016

Rhyu, Im Joo Korean Society of Microscopy 2017 Applied microscopy Vol.47 No.1

Professor Yoshinori Ohsumi received the 2016 Nobel Prize in Physiology or Medicine for his contribution to autophagy research, which was first studied using electron microscopy. To celebrate and commemorate this historical moment, I describe the role of electron microscopy in autophagy research and suggest a role for next-generation electron microscopy in this research field.

High Voltage Electron Microscopic Image of Red Blood Cell in the Blood Vessel of Mouse Brain

Rhyu, Im Joo Korean Society of Microscopy 2017 Applied microscopy Vol.47 No.2

Tottering/leaner 생쥐 소뇌에서 조롱박세포의 가지돌기가시와 평형섬유염주사이의 신경연접 가소성

유임주(Im Joo Rhyu),박창현(Chang Hyun Park),전용혁(Yong Hyuck Chun),서영석(Young-Suk Suh) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

Tottering 생쥐는 운동실조증, 소발작, 간헐성근경련과 유사한 운동장애등의 신경학적 증상을 보이는 변종생쥐이다. tottering (tg) 유전인자는 생쥐의 8번 염색체에 위치하는 것으로 알려져 있으며 보통염색체 열성으로 분류된다. 최근 tottering/leaner 생쥐의 전압의존성칼슘채널 α1A subunit 유전자 변이가 보고되었으며 사람의 보통염색체 우성 소뇌성운동실조증 (Autosomal dominant cerebellar ataxia: SCA6), 가족성반신불수성편두통 (Familial hemiplegic migraine) 및 제2 형간헐성운동실조증 (Episodic ataxia type-2) 등의 질환 모델로서 활용될 수 있게 되었다. 또한 이들 생쥐의 소뇌 분자층에서 과립세포 평행섬유와 조롱박세포 가지돌기가시 사이의 신경연접 가소성이 관찰되었다. 이에 본 연구는, tottering/leaner 생쥐에서 소뇌 분자층의 신경연접을 운동실조가 나타나는 시기에 따라 조롱박세포 가지돌기가 시극돌기와 과립세포 평형섬유팽대사이의 신경연접을 전자현미경을 이용하여 관찰하고, 전압의존성칼슘채널 α1A subunit 이상으로 유발된 세포내 전해질 변화가 조롱박세포의 형태에 미치는 영향을 이해하기 위해 성숙한 tottering/leaner 생쥐의 소뇌를 항 calbindin-28 kd 면역염색을 이용하여 관찰하였다. tottering/leaner 잡종생쥐의 조롱박세포에서 가지돌기가시는 정상대조군에 비하여 세포쪽부위에서 나오는 것이 많이 관찰 되었으며 축삭은 드물지 않게 축삭염주가 관찰되었다. 조롱박세포의 가지돌기가시와 과립세포의 평행섬유염주사이 신경연접에서 3개 이상의 신경연접은, tottering 생쥐에서는 30일군에서, tottering/leaner 잡종에서는 18일군에서, leaner 생쥐에서는 15일군에서 쉽게 관찰되었다. 이상에서 관찰된 형태학적 소견들은 이들 변종생쥐들의 운동실조증과 연관되어 있는 것으로 생각된다. The tottering (tg/tg) is neurologic mutant mouse exhibiting three neurological disorders: ataxia, petit mal-like absence seizures and myoclonic intermittent movement disorder. The tottering mouse carries an autosomal recessive single gene mutation on chromosome 8. The leaner (tgla) and Nagoya rolling (tgrol) are another two alleles of the tottering (tg). The combination of two mutant (tottering and leaner) produces compound heterozygous, tottering/leaner (tg/tgla) mouse. The genetic etilogy of the tottering and leaner was identified to be a mutation in voltage-dependent calcium channel α1A subunit. It made us link these animal model to human neurologic disease such as autosomal dominant cerebellar ataxia (SCA6), familial hemiplegic migraine and episodic ataxia type-2. The different onset and severity of neurological symptom of these three mutants (tg/tg, tg/tgla, tgla/tgla) offer good scale to analysis of pathophysiolgy of the neurologic disorder. Altered synapase between parallel fiber varicosity and dendritic spines of Purkinje cell was observed in adult tottering and leaner mice. Through the electron microscopic observation and anticalbindin-28 kd immunohistochemistry, we anaylzed not only the relationship between neurologic symptoms and synaptic plasticity around the ataxic onset of tottering, leaner and tottering leaner double mutation but also Purkinje cell morphology affected by voltage-sensitive calcium channel α1A subunit mutation in totterring mouse. Purkinje cell dendritic spines from proximal dendrites and axonal swellings of Purkine cell were observed frequently in wild type mice. The first apperance point of altered synapse based on semi-quantitative analysis was postnatal 15 days in leaner, postnatal 18 days in totering/leaner double mutation, and 30 days in tottering. These data suggest that altered synapse is associated with ataxia in tottering and leaner mice. Further study is needed to determine whether altered synapse is primary cause of ataxia.

신경병증성 통증이 유도된 척수에서 Protein Kinase C-γ mRNA 발현

유임주(Im Joo Rhyu),나흥식(Heung Sik Na),성백길(Backil Sung),강윤희(Yoon Hee Kang),김 현(Hyun Kim),서영석(Young-Suk Suh) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.6

신경병증성 통증은 신경손상 후, 평상시에는 무해한 자극에도 비정상적으로 신경계가 과민하게 반응하여 나타나는 만성 병적 통증이다. 현재까지 알려져 있는 기전은 손상된 신경으로부터의 비정상적인 정보가 척수후각세포를 민감화시켜 통증이 나타난다는 것이다. 최근 PKC-γ가 척수후각세포의 민감화에 관여한다는 보고가 있어, 신경병증성통증 모델 흰쥐의 척수에서 PKC-γ mRNA 발현을 in situ 조직화학법을 이용하여 관찰하였다. 손상된 쪽과 건강한 쪽의 척수후각 모두에서 PKC-γ mRNA 발현을 관찰할 수 있었으나, 양쪽의 발현에서 통계적으로 유의한 차이는 보이지 않았다. 이러한 결과 신경병증성 통증 유발에 PKC-γ mRNA 자체 보다는 PKC-γ 단백이나, 그 기능적 활성화가 중요한 역할을 할 수 있음을 시사한다. Partial peripheral nerve injury occasionally results in neuropathic pain, including spontaneous burning pain and increased sensitivity to sensory stimuli such as hyperalgesia and allodynia. The pathophysiological mechanisms underlying this disease are poorly understood and the available treatments unsatisfactory. Presently, the neuropathic pain is believed to result from an increase in the excitability of the dorsal horn neurons (central sensitization), which is induced by abnormal signals from injured afferents. PKC-γ is known to play a pivotal role in central sensitization following peripheral nerve injury. In the present study, we examine the expression of PKC-γ mRNA of the spinal dorsal horn after neuropathic injury. There was no significant difference of PKC-γ mRNA between lesion and control sides. These results suggest that PKC-γ mRNA is not a key factor for the generation of neuropathic pain.

최근 대한체질인류학회지에 출판된 “레오나르도 다빈치의 해부학 업적과 사고”에 대한 의견

유임주(Im Joo Rhyu) 대한체질인류학회 2016 해부·생물인류학 (Anat Biol Anthropol) Vol.29 No.4