Optimization of patch for patchworked b-pillar and its verification

I. B. Shim(심인보),J. H. Kim(김재홍),M. S. Ahn(안민수),P. K Seo(서판기),D. C. KO(고대철),B. M. Kim(김병민) 한국소성가공학회 2015 한국소성가공학회 학술대회 논문집 Vol.2015 No.10

Whole genome de novo sequencing and genome annotation of the world popular cultivated edible mushroom, Lentinula edodes

Shim, D.,Park, S.G.,Kim, K.,Bae, W.,Lee, G.W.,Ha, B.S.,Ro, H.S.,Kim, M.,Ryoo, R.,Rhee, S.K.,Nou, I.S.,Koo, C.D.,Hong, C.P.,Ryu, H. Elsevier Science Publishers 2016 Journal of biotechnology Vol.223 No.-

<P>Lentinula edodes, the popular shiitake mushroom, is one of the most important cultivated edible mushrooms. It is used as a food and for medicinal purposes. Here, we present the 46.1 Mb draft genome of L. edodes, comprising 13,028 predicted gene models. The genome assembly consists of 31 scaffolds. Gene annotation provides key information about various signaling pathways and secondary metabolites. This genomic information should help establish the molecular genetic markers for MAS/MAB and increase our understanding of the genome structure and function. (C) 2016 Elsevier B.V. All rights reserved.</P>

Baicalin improves chronic corticosterone-induced learning and memory deficits via the enhancement of impaired hippocampal brain-derived neurotrophic factor and cAMP response element-binding protein expression in the rat

Lee, B.,Sur, B.,Shim, I.,Lee, H.,Hahm, D. H. Springer Science + Business Media 2014 Journal of Natural Medicines Vol.68 No.1

The purpose of this study was to examine whether baicalin (BAI) improves spatial cognitive impairments induced in rats following the repeated administration of the exogenous stress hormone corticosterone (CORT). The effect of BAI on the hippocampal expression of brain-derived neurotrophic factor (BDNF) and cAMP response element-binding protein (CREB) was also investigated. For 21 days, male rats received daily doses of BAI (20, 50, and 100 mg/kg, i.p.) 1 h prior to a CORT (40 mg/kg) injection. The daily administration of BAI improved memory impairment as measured by the passive avoidance test and reduced the escape latency for finding the platform in the Morris water maze test. Additionally, as assessed by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analysis, the administration of BAI also significantly alleviated memory-associated decreases in the expression levels of BDNF and CREB proteins and mRNAs in the hippocampus. These results demonstrate that the administration of BAI prior to high-dose exogenous CORT results in significant neuroprotective activity against neuronal impairment and memory dysfunction in rats. Thus, these findings suggest that BAI might be useful as a therapeutic agent in various neurodegenerative diseases for the improvement of cognitive function. This likely occurs through the regulation of BDNF and CREB expression.

Ginsenoside Rb1 rescues anxiety-like responses in a rat model of post-traumatic stress disorder

Lee, B.,Sur, B.,Cho, S. G.,Yeom, M.,Shim, I.,Lee, H.,Hahm, D. H. Springer Science + Business Media 2016 Journal of Natural Medicines Vol.70 No.2

<P>Single prolonged stress (SPS), a rat model of post-traumatic stress disorder (PTSD), induces alterations in the hypothalamic-pituitary-adrenal axis. Korean red ginseng, whose major active component is ginsenoside Rb1 (GRb1), is one of the widely used traditional anxiolytics. However, the efficacy of GRb1 in alleviating PTSD-associated anxiety-like abnormalities has not been investigated. The present study used several behavioral tests to examine the effects of GRb1 on symptoms of anxiety in rats after SPS exposure and on the central noradrenergic system. Male Sprague-Dawley rats received GRb1 (10 or 30 mg/kg, i.p., once daily) during 14 days of SPS. Daily GRb1 (30 mg/kg) administration significantly increased the number and duration of open-arm visits in the elevated plus maze (EPM) test, reduced the anxiety index, increased the risk assessment, reduced grooming behaviors in the EPM test, and increased the total number of line crossings of an open field after SPS. The higher dose of GRb1 also blocked SPS-induced decreases in hypothalamic neuropeptide Y expression, increases in locus coeruleus tyrosine hydroxylase expression, and decreases in hippocampal mRNA expression of brain-derived neurotrophic factor. These findings suggest that GRb1 has anxiolytic-like effects on both behavioral and biochemical symptoms similar to those observed in patients with PTSD.</P>

뇌막염의 임상적 연구

박충서,임백서,심재용 中央醫學社 1973 中央醫學 Vol.24 No.4

Total 62 meningitis cases since 1958 to 1971 are analyzed. T.B. origin are 13 cases,. bacterial 4, syphilitic 3, toxic 1, and unknown origin 41 cases. Age is mainly 2nd and 3rd decades. Male female ratio is approximately equal. Duration of the disease is mainly 1 month, esp. within 2 weeks. The main symptoms are headache, fever, vomiting, stupor, nausea, and dizziness and main signs are stiff neck, Kernig sign, weak deep reflexes, Babinski sign, and semi-coma. The cerebrospinal fluid showed often confusing picture for T. B. meningitis. Medications for both T. B. and bacterial meningitis together resulted in about 67. 7% improvement (probably cure) and the worsening or expiring cases are those who were too late for treatment.

약침이 반복적인 코카인 투여로 인한 행동적 민감화 현상에 미치는 효과

이봄비,양재하,함대현,이혜정,심인섭,Lee, B.B,Yang, C.H.,Hahm, D.H.,Lee, H.J.,Shim, I.S. 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.1

Substantial evidence suggests that behavioral and reinforcing effects of cocaine can be mediated by the mesolimbic dopaminergic system. It has been shown that repeated injections of cocaine produce increase in locomotor activity, expression of the immediate-early gene, c-fos in the nucleus accumbens (NAc), which was one of the main dopaminergic terminal areas. Herbal-acupuncture as a therapeutic intervention has been widely used for the treatment of many functional disorders such as drug abuse. Coptidis Rhizoma (CR) and its main component, berberine (BER) were selected as herbal medicine of herbal-acupuncture. Both medicines have been known to have the therapeutic effect on the central nervous system. In order to investigate the effects of CR and BER herbalacupuncture at shenmen (HT7) point (CR/H and BER/H) on the cocaine-induced behavioral sensitization, the influence of CR/H and BER/H on repeated cocaine-induced locomotor activity, the change of c-Fos expression in the brain by immunohistochemistry were examined. Male SD rats were given CR/H (0.4mg/kg) and BER/H (0.1mg/kg) 30 min before daily injections of cocaine hydrochloride (15mg/kg. i.p.) 10 days. After 3 days withdrawal, rats received a challenge injection of cocaine (15mg/kg, i.p.). Systemic challenge with cocaine produced much larger increased locomotor activity, accumbal Fos-like immunoreactivity in the NAc. Pretreatment with CR/H and BER/H significantly inhibited cocaine-induced locomotor activity, the change of c-Fos expression in the rats. Our data demonstrated that the inhibitory effects of cocaine-induced behavioral sensitization by CR/H and BER/H were closely associated with the reduction of presynaptic dopamine release in the NAc. These results suggest that CR/H and BER/H can be effectively applied to cocaine addiction.

Diisopropylamine dichloroacetate의 分析에 關한 硏究

李海彬,崔秉箕,金吉洙,沈壽一,梁美瑛 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

綜合消化酵素劑의 藥效 및 藥劑學的 硏究

李海彬,崔秉箕,沈壽一,金吉洙,高守烈 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

항진균제의 경피흡수형 제제의 효력시험

김봉수,유재일,이영선,심수경,최연화,차정옥,남희정 국립보건연구원 2000 국립보건원보 Vol.37 No.-

원주지역 남은 음식물의 계절별 성분 함량 및 비육돈에 대한 건조 남은 음식물 급여 효과

채병조,주지환,심영호,권일경,김상헌 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.3

본 연구는 남은 음식물(FW)의 성분변이를 조사하고, 그것이 급여시 비육돈의 성장과 육질에 미치는 영향을 조사하기 위하여 실시하였다. 사료화를 위해 기초자료로 활용하고져 FW를 1년간 (월 6회) 수집하였다. 8주간의 사양시험을 위해 삼원교잡종(LxYxD) 비육돈 (54.80±4.60㎏) 117두를 공시하였다 (3처리 3반복, 반복당 13두). 처리는 대조구 (옥수수-대두박 위주사료), 건조 방법에 따른 단순 건조 (simple dry : SD) 및 진공발효 (vacuum fermentation: VF)로서 구분하였다. FW의 건물기준으로 에너지, 조단백질, 조지방, 화분, 칼슘 및 인의 4계절 평균치는 각각 5,11kcal/㎏, 22.92%, 14.31%, 15.48%, 2.7% 및 1.05%였다. 에너지와 단백질 함량은 각각 겨울과 여름에 가장 높았다 (p<0.05). 건조한 FW의 유산균 함량은 SD와 VF 처리간에 차이가 없었다. 사양성적에서는 ADG에서 대조구가 FW이 첨가된 사료에 비해 유의적으로 높았으나 (p<0.05), FCR에서는 처리간에 차이가 없었다. SD와 FD와의 생산성 비교에서는 유의차가 인정되지 않았다. 지육율, 등지방두께, 그리고 기타육질(색깔, drip loss 및 TBARS)에서 처리간에 차이가 없었다. 전 시험기간의 증체 ㎏당 사료비를 살펴보면, FW가 포함된 사료급여군이 대조구에 비해 낮았다. 이상의 결과를 요약해 보면, 남은 음식물을 건조한 후 사료에 20% 이내를 첨가하여 펠렛사료로 급여할 경우 비육돈의 사료비를 절감할 수 있을 것으로 사료된다. 그러나 FW의 가공시 FD공정은 필요치 않을 것으로 사료된다. A study was conducted to evaluate seasonal variations in chemical composition of food waste (FW) and its feeding effects on growth performance and pork quality in finishing pigs. FW was collected for 1 year ( 6 times a month ) to establish a database for use of FW as a feed ingredient. For a feeding trial (8 weeks), a total of 117 pigs (L×Y×D; 54.80±4.60㎏) were used to evaluate the processing effects of FW. Treatments were: Control (a corn-soybean meal diet without FW), simple dried FW (SD) and vacuum fermented FW (VF). The gross energy, crude protein, crude fat, ash, calcium and phosphorus in FW (DM, average of 4 seasons) were 5,111㎉/㎏, 22.92%, 14.31%, 15.48%, 2.7% and 1.05%, respectively. Among seasons, the energy and crude protein contents were the highest (p<0.05) in winter and summer, respectively. In lactic acid bacterial counts, there was no difference between SD and VF. Pigs fed the control diet grew faster (p<0.05) than those fed diets containing food waster, but not feed conversion ratio. There were no differences in production traits between SD and VF. No differences were also found in dressing percentage, backfat thickness, and pork quality (color, drip loss and TBARS) among treatments. The feed cost (￦/㎏ body weight) was lower in pigs fed FW than those fed a control diet. In conclusion, a pelleted diet containing food waste less than 20% would reduce feed cost in finishing pigs. However, it seems that a vacuum fermentation of food waste is not necessary for diet processing.