Evaluation of Biological Performance in a Newly Developed Single-use Bioreactor System, CELBIC System, for Large Scale Culture of Recombinant CHO Cell

Hyunwoo KIM,Junghyun CHO,Duk Jae OH 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

The usage of single-use bioreactors for cell culture has been increasing continuously, as the bio-industry of biopharmaceuticals (proteins, antibodies, cell therapies and gene therapies products) has expanded dramatically during recent decades. The SUBs (single-use bioreactors) have several advantages compared with the conventional stainless steel based bioreactors, such as flexibility in scale, low risk of contamination, reduced labors for cleaning/sterilization, and validation/regulation issues. In this study, a newly developed cell culture system, CELBIC system, driven by multi-directional rocking movement is introduced and evaluated its physical and biological performances as a cell culture device. The recombinant cell line derived from CHO-S cell was cultured for producing IFN-beta(interferon beta) of 50 liters working volume in batch culture. The CELBIC system was controlled for making orbitally shaken motion with 10 ° tilt angle and 20 rpm. ELISA and western blot were used for analysis of IFN-beta concentration. From these experiments, the maximum viable cell density was 8.81 x 10⁶ cells/mL in the CELBIC system and this result was the highest value among all groups of experiment. The maximum viable cell density and the IVCD(Integral of Viable Cell Density) were increased up to 1.42 folds and 1.47 folds respectively compared to a control group. In summary, since the CELBIC system driven by orbital movement provides sufficient and excellent conditions for cell cultures, it can be an attractive candidate for suspension cell culture device to produce biopharmaceutics, in particular, antibodies or therapeutic proteins from recombinant CHO cells.

영웅소설의 운명론과 그 위안 - 자기 확인이 주는 위안의 기능을 중심으로 -

조현우 ( Cho¸ Hyunwoo ) 한국고소설학회 2020 古小說 硏究 Vol.49 No.-

이 글에서는 비범한 능력을 가진 천상계의 존재가 적강해 예정된 성공을 얻는 이야기인 영웅소설이 어떻게 봉건적 신분질서 속에서 고통 받던 평민 이하 계층에게 인기를 얻을 수 있었는가를 집중적으로 검토했다. 영웅이 삶의 각 국면에서 겪는 사태는 그가 적강할 때부터 이미 정해진 경로에 따라 진행된다. 그런 점에서 영웅소설에 나타나는 천정은 예정론의 관점에서 접근할 수 있다. 모든 것이 이미 다 정해져 있다는 예정론의 논리에 따르면, 주체가 현재 하는 선택은 과거에 무엇이 예정되어 있었는가를 결정한다. 선택 자체가 운명이라면, 주체가 무엇을 결정하든 그것은 곧 자신의 운명을 선택하는 일이 된다. 이때 수동적이고 무력해 보였던 운명과 주체의 관계가 역전된다. 이러한 관점은 영웅소설의 운명론에 대해 지금까지와는 다른 해석을 가능하게 해준다. 영웅은 지상계 너머의 그 무엇을 추구하지 않는다. 천상계는 그가 지금 여기에서 사는 삶에 정당성을 부여한다. 그러한 정당성은 영웅에게 주어진 운명을 선택에 따른 실현과 그로부터 비롯되는 자기 확신으로 변화시킨다. 영웅소설 장르에 공통적으로 등장하는 운명론은 관습적인 설정을 넘어서서 향유층에게 일정한 위무를 제공했다. 이 글에서는 바로 이러한 운명론의 전유로 인해 영웅소설이 평범하게 타고난 독자들에게 운명을 받아들여 자유롭게 살라는 위안의 서사로 기능할 수 있었다고 보았다. 또 영웅소설이 주는 위안에 대한 고찰을 통해 현재 유행하는 수저론과 비현실적일 정도로 비범한 인물들의 이야기인 슈퍼 히어로 서사의 인기를 함께 설명할 수 있다고 주장했다. 영웅소설이나 슈퍼 히어로 서사가 독자에게 위안을 줄 수 있다는 이 글의 주장이 이러한 서사가 언제나 독자에게 긍정적 영향을 미친다는 의미는 아니다. 그러나 예정된 궤도대로 사는 영웅의 이야기가 가진 매력이 운명론이 주는 위안의 기능과 깊은 관계가 있다는 점은 강조될 필요가 있다. In this article, I explored the reason why Hero Novels could be popular with common people during the Joseon Dynasty. In these novels, the hero who came from heaven achieves a big success without difficulty, because everything in his earthly life was predetermined since he fell down from heaven. However, if everything is already decided, what the hero does through his choice in the present decides what was predetermined in the past. If everything the hero does can't escape his fate, it means he chooses his destiny no matter what he chooses. This paradox included in the predestination theory transforms the passiveness included in fatalism into the infinite activeness of the subject. In this article, I argued that there is a possibility to newly understand the fatalism of hero novels by actively applying the paradox of predestination. Now the fate given to the hero not only justifies his choices and actions, but also provides him with the sense of self-confirming. The hero novels could be read as a story that provided consolation to a wide range of readers in the Joseon Dynasty through this sense of self-confirming, I believe the consolation of hero novels provides a certain explanation of why superhero stories are so popular in Korea at present. I don't want to argue that the consolation of hero novels or superhero stories to readers always works positively. However, it needs to be emphasized that the story of a hero who lives according to his naturalbor destiny can provide comfort to those who suffer in reality.

Formal Verification in Commercial Environments

Hyunwoo Cho 대한전자공학회 1996 대한전자공학회 학술대회 Vol.1996 No.7

Culture of Recombinant CHO Cells in a Newly Developed Single-use Bioreactor System, CEL-BIC™

Hyunwoo KIM,Hyanghee CHO,Jeong Hwan KIM,Duk Jae OH 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.4

In situ fabrication of nanohybrid carbon/polyamide film providing robust binding and conductive network in silicon anode for lithium-ion battery

Cho, Hyunwoo,Kim, Kyungsu,Park, Cheol-Min,Jeong, Goojin Elsevier 2019 Journal of Power Sources Vol.410 No.-

<P><B>Abstract</B></P> <P>Engineering of a composite electrode containing Si-based anode materials is important for the practical applications of Si-based lithium-ion batteries. In this work, a hybrid composite anode of silicon/carbon/polymer was fabricated in situ by optimized heat treatment of the coating slurry containing silicon power and polyamic acid polymer, without adding any carbon agent. The resulting electrode has a three-component (silicon-carbon-polyamide) composite structure, wherein the nanohybrid carbon/polyamide matrix is in-situ generated from polyamic acid by optimized heat treatment. The hybrid Si composite electrode exhibits robust binding and conductive properties, and delivers promising battery performance even for high contents of the micron-sized Si powder (∼95 wt%). Consequently, the proposed method for Si anode engineering is another effective way to manufacture advanced lithium-ion batteries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A Si/C/polyamide composite is fabricated from Si and polyamic acid coat. </LI> <LI> The composite exhibits mechanically and electrically robust network. </LI> <LI> The battery performance is resultingly promising. </LI> <LI> This fabrication method can be an effective way for advanced LIB. </LI> </UL> </P>

A processor with an improved ARM7 core and peripherals

Hyunwoo Cho,Kyungchol Huh,Seungwon Song,Taehoon Kim,WonTae Choi,Haksun Kim,Jusung Park 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

This paper is about designing RISC Processor which is compatible with ARM7 and has partial functions improved and added. The existing ARM7 Processor designed with a latch-based pipeline structure has a problem of time violation at the back-end. Also, its built-in 32 x 8-bit multiplier requires many cycles. In order to solve these problems of the ARM7 Processor, in this study, a flip-flop-based pipeline structure was designed, and in the aspect of (calculation time) x (number of gates), a 32x16 multiplier which is superior to the existing one was designed. Additionally, the new processor includes AMBA BUS, Audio codec interface, MMU (Memory Management Unit), and 192Kbyte RAM. After FPGA test was performed on the designed processor, a chip was fabricated with 0.18 ㎛ CMOS processing. Through application program tests such as unit instruction test, instruction combination test, ADPCM(Adaptive Differential Pulse Code Modulation), SOLA(synchronized-overlap-add), MP3 decoder, it was confirmed that the fabricated chip is compatible with ARM7TDMI. Furthermore, it was confirmed that the designed 32-bit RISC Processor performs all application programs in the unit of MIPS (million instructions per second), which is much better than the previous processor.

Hepatoprotective effect of germinated soybean embryo extracts in HFD-fed obese mice

Hyunwoo Cho,Chawon Yun,Young-Suk Jung 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

In vivo action of RNA G-quadruplex in phloem development

( Hyunwoo Cho ),( Hyun Seob Cho ),( Ildoo Hwang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.11

Phloem network integrates cellular energy status into post-embryonic growth, and development by tight regulation of carbon allocation. Phloem development involves complicated coordination of cell fate determination, cell division, and terminal differentiation into sieve elements (SEs), functional conduit. All of these processes must be tightly coordinated, for optimization of systemic connection between source supplies and sink demands throughout plant life cycle, that has substantial impact on crop productivity. Despite its pivotal role, surprisingly, regulatory mechanisms underlying phloem development have just begun to be explored, and we recently identified a novel translational regulatory network involving RNA G-quadruplex and a zinc-finger protein, JULGI, for phloem development. From this perspective, we further discuss the role of RNA G-quadruplex on post-transcriptional control of phloem regulators, as a potential interface integrating spatial information for asymmetric cell division, and phloem development. [BMB Reports: Perspective 2018; 51(11): 547-548]

<i>Gracilimonas rosea</i> sp. nov., isolated from tropical seawater, and emended description of the genus <i>Gracilimonas</i>

Cho, Yirang,Chung, Hyunwoo,Jang, Gwang Il,Choi, Dong Han,Noh, Jae Hoon,Cho, Byung Cheol International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.11

<P>A Gram-staining-negative, non-motile, spore-forming, rod-shaped, marine bacterial strain, CL-KR2<SUP>T</SUP>, was isolated from tropical seawater near Kosrae, an island in the Federated States of Micronesia. Analysis of the 16S rRNA gene sequence of strain CL-KR2<SUP>T</SUP> revealed a clear affiliation with the genus <I>Gracilimonas</I>. Based on phylogenetic analysis, strain CL-KR2<SUP>T</SUP> showed the closest phylogenetic relationship to <I>Gracilimonas tropica</I> CL-CB462<SUP>T</SUP>, with 16S rRNA gene sequence similarity of 96.6 %. DNA–DNA relatedness between strain CL-KR2<SUP>T</SUP> and <I>G. tropica</I> CL-CB462<SUP>T</SUP> was 6.7 % (reciprocal 9.5 %). Strain CL-KR2<SUP>T</SUP> grew in the presence of 1–20 % sea salts and the optimal salt concentration was 3.5–5 %. The temperature and pH optima for growth were 35 °C and pH 7.5. The major cellular fatty acids (≥10.0 %) of strain CL-KR2<SUP>T</SUP> were iso-C<SUB>15 : 0</SUB>, summed feature 3 (iso-C<SUB>15 : 0</SUB> 2-OH and/or C<SUB>16 : 1</SUB>ω7<I>c</I>) and iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and the only isoprenoid quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, two unidentified glycolipids and two unidentified lipids. The genomic DNA G+C content of strain CL-KR2<SUP>T</SUP> was 43.2 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-KR2<SUP>T</SUP> could be distinguished from the only member of the genus <I>Gracilimonas</I> with a validly published name. Thus, strain CL-KR2<SUP>T</SUP> should be assigned to a novel species in the genus <I>Gracilimonas</I>, for which the name <I>Gracilimonas rosea</I> sp. nov. is proposed. The type strain is CL-KR2<SUP>T</SUP> ( = KCCM 90206<SUP>T</SUP> = JCM 18898<SUP>T</SUP>).</P>

Partially Carbonized Poly (Acrylic Acid) Grafted to Carboxymethyl Cellulose as an Advanced Binder for Si Anode in Li-ion Batteries

Cho, Hyunwoo,Kim, Kyungsu,Park, Cheol-Min,Jeong, Goojin The Korean Electrochemical Society 2019 Journal of electrochemical science and technology Vol.10 No.2

To improve the performance of Si anodes in advanced Li-ion batteries, the design of the electrode plays a critical role, especially due to the large volumetric expansion in the Si anode during Li insertion. In our study, we used a simple fabrication method to prepare Si-based electrodes by grafting polyacrylic acid (PAA) to a carboxymethyl cellulose (CMC) binder (CMC-g-PAA). The procedure consists of first mixing nano-sized Si and the binders (CMC and PAA), and then coating the slurry on a Cu foil. The carbon network was formed via carbonization of the binders i.e., by a simple heat treatment of the electrode. The carbon network in the electrode is mechanically and electrically robust, which leads to higher electrical conductivity and better mechanical property. This explains its long cycle performance without the addition of a conducting agent (for example, carbon). Therefore, the partially carbonized CMC-g-PAA binder presented in this study represents a new feasible approach to produce Si anodes for use in advanced Li-ion batteries.