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Effects of Hwangryunhaedok-tang on gastrointestinal motility function in mice
Kim, Hyoyeon,Kim, Iksung,Lee, Min Cheol,Kim, Hyun Jung,Lee, Guem San,Kim, Hyungwoo,Kim, Byung Joo Baishideng Publishing Group Inc 2017 WORLD JOURNAL OF GASTROENTEROLOGY Vol.23 No.15
<P><B>AIM</B></P><P>To investigate the effects of Hwangryunhaedok-tang (HHT) on gastrointestinal (GI) motility in mice.</P><P><B>METHODS</B></P><P>The effects of a boiling water extract of HHT (HHTE) on GI motility were investigated by calculating percent intestinal transit rates (ITR%) and gastric emptying (GE) values using Evans Blue and phenol red, respectively, in normal mice and in mice with experimentally induced GI motility dysfunction (GMD). In addition, the effects of the four components of HHT, that is, Gardeniae Fructus (GF), Scutellariae Radix (SR), Coptidis Rhizoma (CR), and Phellodendri Cortex (PC), on GI motility were also investigated.</P><P><B>RESULTS</B></P><P>In normal ICR mice, ITR% and GE values were significantly and dose-dependently increased by the intragastric administration of HHTE (0.1-1 g/kg). The ITR% values of GMD mice were significantly lower than those of normal mice, and these reductions were significantly and dose-dependently inhibited by HHTE (0.1-1 g/kg). Additionally, GF, CR, and PC dose-dependently increased ITR% and GE values in normal and GMD mice.</P><P><B>CONCLUSION</B></P><P>These results suggest that HHT is a novel candidate for the development of a gastroprokinetic agent for the GI tract.</P>
Hye-Yeon Han,Bong-Soo Bark,Hyung Joon Kim,Seung-Hwa Jeong,Jiyeon Kim,Sung-Hee Jeong,Gyoo Cheon Kim,Dae-Seok Hwang,Uk-Kyu Kim,Hyungwoo Kim,Mi Heon Ryu 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.6
The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.
Kim, Miyoung,Kim, Hanna,Ryu, Jeonghyun,Jo, Suzy,Lee, Guemsan,Ryu, Mi Heon,Kim, Hyungwoo,Cho, Su In Medknow PublicationsMedia Pvt Ltd 2014 Pharmacognosy magazine Vol.10 No.38
<P><B>Background:</B></P><P>The slough shed of <I>Cryptotympana atrata</I> Fabricius is widely used to treat skin diseases in China, Japan, and Korea.</P><P><B>Objective:</B></P><P>To investigate the anti-inflammatory effects of <I>C. atrata</I> on contact dermatitis.</P><P><B>Materials and Methods:</B></P><P>We investigated the effects of <I>C. atrata</I> methanol extract (MECA) on ear swelling, histophathological changes and cytokine production in 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) mice.</P><P><B>Results:</B></P><P>Topical application of MECA effectively inhibited enlargement of ear swelling (30 and 100 μ/ear, <I>P</I> < 0.05; 300 μg/ear, <I>P</I> < 0.01). MECA treatment also inhibited hyperplasia, spongiosis (100 and 300 μg/ear, <I>P</I> < 0.001), and immune cell infiltration (30 μg/ear, <I>P</I> < 0.05; 100 and 300 μg/ear, <I>P</I> < 0.001) induced by DNFB. In addition, treatment with MECA suppressed the increase in the levels of TNF-α (<I>P</I> < 0.05), IFN-g (3, 100 μg/ear, <I>P</I> < 0.05; 300 μg/ear, <I>P</I> < 0.01), and IL-6 (100 μg/ear, <I>P</I> < 0.05; 300 μg/ear, <I>P</I> < 0.01) production.</P><P><B>Conclusion:</B></P><P>These data suggest that MECA has the potential for use in the treatment of inflammatory skin diseases, including CD. Moreover, the results presented herein indicate that anti-inflammatory actions of MECA are mediated by decreasing production of TNF-α, IFN-γ, and IL-6 in inflamed tissues.</P>
Effects of astaxanthin on dinitrofluorobenzene-induced contact dermatitis in mice
KIM, HYUNGWOO,AHN, YONG-TAE,LEE, GUEM SAN,CHO, SU IN,KIM, JONG-MYOUNG,LEE, CHU,LIM, BYUNG KWAN,JU, SEONG-A,AN, WON GUN SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3
<P>Astaxanthin (AST) is known to exhibit antioxidative and antitumor properties, therefore, the present study investigated its other potential medical applications. AST was observed to exhibit anti?allergic and anti?inflammatory effects in a dinitrofluorobenzene (DNFB)?induced contact dermatitis (CD) mouse model and RBL?2H3 cell lines. The topical application of AST effectively inhibited the enlargement of ear thickness and increase in weight, which occurred following repeated application of DNFB. Furthermore, topical application of different concentrations of AST inhibited inflammatory hyperplasia, edema, spongiosis, and the infiltration of mononuclear cells and mast cells in the ear tissue. In addition, the levels of TNF?α and IFN?γ produced were decreased by application of AST in vivo, and treatment of RBL?2H3 cells with AST inhibited the release of histamine and β?hexosaminidase in vitro. Taken together, these data suggested that AST may be used to treat patients with allergic skin diseases through a mechanism, which may be associated with that involved in anti?inflammatory or anti-allergic activities.</P>
Hyungwoo Kim,Jangwon Lee,Jongsuh Lee,Jaeyub Hyun,Semyung Wang IEEE 2016 IEEE transactions on magnetics Vol.52 No.3
<P>In this paper, a magnetic resonator for wireless energy transfer system is designed using topology optimization based on an electromagnetic wave analysis. To analyze the magnetic resonator, the finite-difference time-domain method in a 2-D transverse magnetic mode is used with a Gaussian pulse source and perfectly matched layers. The topology optimization using solid isotropic material with the penalization method of a magnetic resonator is conducted to maximize magnetic energy. After these approaches, the fast Fourier transform is used to obtain the response of the magnetic resonator system over a wide range of frequencies.</P>
Kim, Hyungwoo,Chang, Ji Young Royal Society of Chemistry 2011 Soft matter Vol.7 No.18
<P>A trimetallic disc-like europium(<SMALL>III</SMALL>) complex having an organogelation and film-forming property was prepared. The complex showed a much higher PL intensity of the <SUP>5</SUP>D<SUB>0</SUB> → <SUP>7</SUP>F<SUB>2</SUB> transition in the gel state than in the solution state at the same concentration (1 wt%).</P> <P>Graphic Abstract</P><P>A disc-like europium(<SMALL>III</SMALL>) complex having an organogelation and film-forming property was prepared. The complex showed a much higher PL intensity in the gel state than in the solution state. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1sm05669g'> </P>
Kim, Hyun Jung,Lee, Guem San,Kim, Hyungwoo,Kim, Byung Joo WJG Press 2017 WORLD JOURNAL OF GASTROENTEROLOGY Vol.23 No.29
<P><B>AIM</B></P><P>To investigate the effects of a water extract of Hwangryunhaedok-tang (HHTE) on the pacemaker potentials of mouse interstitial cells of Cajal (ICCs).</P><P><B>METHODS</B></P><P>We dissociated ICCs from small intestines and cultured. ICCs were immunologically identified using an anti-c-kit antibody. We used the whole-cell patch-clamp configuration to record the pacemaker potentials generated by cultured ICCs under the current clamp mode (<I>I</I> = 0). All experiments were performed at 30 °C-32 °C</P><P><B>RESULTS</B></P><P>HHTE dose-dependently depolarized ICC pacemaker potentials. Pretreatment with a 5-HT<SUB>3</SUB> receptor antagonist (Y25130) or a 5-HT<SUB>4</SUB> receptor antagonist (RS39604) blocked HHTE-induced pacemaker potential depolarizations, whereas pretreatment with a 5-HT<SUB>7</SUB> receptor antagonist (SB269970) did not. Intracellular GDPβS inhibited HHTE-induced pacemaker potential depolarization and pretreatment with a Ca<SUP>2+</SUP>-free solution or thapsigargin abolished the pacemaker potentials. In the presence of a Ca<SUP>2+</SUP>-free solution or thapsigargin, HHTE did not depolarize ICC pacemaker potentials. In addition, HHTE-induced pacemaker potential depolarization was unaffected by a PKC inhibitor (calphostin C) or a Rho kinase inhibitor (Y27632). Of the four ingredients of HHT, Coptidis Rhizoma and Gardeniae Fructus more effectively inhibited pacemaker potential depolarization.</P><P><B>CONCLUSION</B></P><P>These results suggest that HHTE dose-dependently depolarizes ICC pacemaker potentials through 5-HT<SUB>3</SUB> and 5-HT<SUB>4</SUB> receptors <I>via</I> external and internal Ca<SUP>2+</SUP> regulation and <I>via</I> G protein-, PKC- and Rho kinase-independent pathways.</P>
Graphene nanonet for biological sensing applications
Kim, Taekyeong,Park, Jaesung,Jin, Hye Jun,Lee, Hyungwoo,Byun, Kyung-Eun,Lee, Chang-Seuk,Kim, Kwang S,Hong, Byung Hee,Kim, Tae Hyun,Hong, Seunghun IOP Pub 2013 Nanotechnology Vol.24 No.37
<P>We report a simple but efficient method to fabricate versatile graphene nanonet (GNN)-devices. In this method, networks of V<SUB>2</SUB>O<SUB>5</SUB> nanowires (NWs) were prepared in specific regions of single-layer graphene, and the graphene layer was selectively etched via a reactive ion etching method using the V<SUB>2</SUB>O<SUB>5</SUB> NWs as a shadow mask. The process allowed us to prepare large scale patterns of GNN structures which were comprised of continuous networks of graphene nanoribbons (GNRs) with chemical functional groups on their edges. The GNN can be easily functionalized with biomolecules for fluorescent biochip applications. Furthermore, electrical channels based on GNN exhibited a rather high mobility and low noise compared with other network structures based on nanostructures such as carbon nanotubes, which was attributed to the continuous connection of nanoribbons in GNN structures. As a proof of concept, we built DNA sensors based on GNN channels and demonstrated the selective detection of DNA. Since our method allows us to prepare high-performance networks of GNRs over a large surface area, it should open up various practical biosensing applications.</P>