김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

한국산 채소류의 Angiotensin Ⅰ converting enzyme 저해작용에 관한 연구

배인영,신현경,양차범 漢陽大學校 韓國生活科學硏究所 1999 韓國 生活 科學 硏究 Vol.- No.17

The renin-angiotensin system is one of the most important of humoral vasoconstrictor and vasodilator machanisms involved in blood pressure regulation. The angiotensin I converting enzyme catalyzes the production of the vasoconstrictor angiotensin II as well as the inactivation of the vasodilator bradykinin and of the enkaphalins. Consequently, many researchers have investigated to prepare and isolate the naturally occuring ACE inhibitors. This study was carried out to investigate the inhibitory activity of ACE from 33 kinds of vegetables. The yield and ACE inhibitory activity of these samples were found to be significantly affected by the condition of extraction. ACE inhibitory activity of most samples were higher in water extract in order with broccoli, Small watercress, sedum, leek, parsley, egg plant, green onion, radish root and perllila leaf exhibiting more than 60%. The yield of soluble solids in water extraction were higher in order with pumpkin, garlic, sedum, onion, broccoli, spinach, cucumber, green onion, korean cabbage and leek exhibiting more than 20%. When dried samples were extracted with various solvents, sedum, broccoli and tomato showed higher ACE inhibition in ethanol extract while parsley, leek, Small watercress, persimmon leaf and garlic showed higher ACE inhibition in methanol extract. Hot water extraction at 100℃ for 30min caused a significant decrease in ACE inhibition as compared with cold water extraction. Thus these results suggest that ACE inhibitor of these samples seems to be water soluble component being heat-unstable.

골격성 Ⅱ급 부정교합자의 악교정 수술 후 설골의 위치와 상기도 변화에 관한 연구

배진성,김경호,박형식,허종기,박광호 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

OSAS(obstructive sleep apnea syndrome) is becoming more and more of importance nowadays. Therefore the study concerning OSAS is very important. Most of the studies which have been reported were comparison of upper airway size and change of skeletal class Ⅰ group and skeletal class Ⅲ group respectively. But, most of the showing OSAS have pharyngeal narrowing combined with more or less distinct maxillary and mandibular deficiency. Therefore the aim of this study is to see posterior airway space and hyoid bone position in patients affected by class Ⅱ malocclusion and treated with orthognathic surgery. we measured the lines between selected upper air way landmarks on lateral cephalometric x-ray films of skeletal class Ⅱ 30 persons who had not been operated yet, were within 2 weeks after operation, were 6 months after operation. And we compared them respectively and analyzed them with pairing t-test, pearson correlation coefficient and multiple regression analysis. The results were as follows. First of all, skeletal class Ⅱ group was increased in all the level of PAS, especially PAS(MK) and PAS related to hyoid bone(H-phW, H-PhW(Me-H)) was significantly decreased within 6 months after operation but that was larger than preoperation. And then the PAS was increased after mandibular advancement surgery. While there was no significant correlation between PAS and surgical methods, there was statistical significance between PAS and a number of surgical movement. At last, the hyoid bone was advanced within 2 weeks after operation but relapsed within the follow up period. In the end of the study, it was turned out that PAS was always wider without distinction of the region after the operation and that there was significant correlation between the moving volume of hard tissues and the changing quantity of PAS( ; how much PAS was changed according to the moving degree of hard tissues). After this, to be based on the study, I consider that OSAS demands further study and that it is necessary to do more practical applications to the patients.

심실성 조기박동의 심전도학적 연구

배형준 서울保健大學 1995 論文集 Vol.15 No.1

A study on the ventricular premature beats was made on 123 cases diagnosed by surface electrocardiographic examination. The results were as follows : 1. Ventricular premature beats were observed in 123 cases of the 5,456 adult patients and the incidence of ventricular premature beats was 2.3%. 2. The ratio of male to female was 44.7% to 55.3%. The most frequent age group was 5th decade, and the incidence of ventricular premature beats rose suddenly over the 5th decade. 3. Atrial fibrillation was one of the most frequent arrhythmias associated with ventricular premature beats, being 24 cases(19.5%) Second most associating arrhythmias was bundle branch block with or without fascicular block, being 9 cases(7.3%). 4. Ventricular premature beats with interpolated beat were 8 cases(6.5%), with retrograde-P wave were 18 cases(14.6%), and end-diastolic ventricular premature beats were 8 cases(14.6 %). 5. 66 cases(53.7%) were classified as having ventricular premature beats originated in the right ventricle, 52 cases(42.3%) were originated in the left ventricle, and 5 cases(4.1%) had septal ventricular premature beats. 6. Ventricular bigeminy were 10 cases(8.1%), ventricular trigeminy were 4 cases(3.3%), and ventricular Quadrigeminy was 1 case(0.8%).

설치장소에 따른 태양열 스털링엔진 발전시스템의 성능예측에 관한 연구

배명환,장형성,강상율 경상대학교 생산기술연구소 2001 工學硏究院論文集 Vol.1 No.-

The simulation analyses of a dish solar power system with stilling engine in this study are applied to system performance prediction in four different test sites; Seoul, Pusan and Cheju in Korea, and Naha in Japan. The effects of difference of concentrator type such as monolithic and stretched-membrane construction on system efficiency are also evaluated. The total amount of generated power for a year depends on the site. However the total maximum system efficiency in every site is approximately 16% and there isn't a striking difference. It is also found that the maximum collector efficiency of stretched-membrane concentrator Is about 3 ∼ 15% lower than that of the monolithic type.

GC 및 활성탄에 의한 알킬 치환 페놀류의 분자면적 추정

배선영,안형신,김건,이동선 서울여자대학교 자연과학연구소 1996 자연과학연구논문집 Vol.7 No.-

Activated carbons were prepared from leaves of Ginkgo biloba by activation process with zinc chloride after carbonization. The specific surface area of this activated carbon measured by BET surface analyzer was obtained as 469.8㎡/g. Adsorption of alkyl substituted phenols on activated carbon was determined using gas chromatography. It was found that molecular area could be estimated using Langmuir's isotherm, specific surface area, and Avogadro's number. Molecular areas for phenol, 2-ethyl phenol, 2,5-dimethyl phenol, 2-isopropyl phenol were 0.0288n㎡, 0.0796n㎡, 0.0384n㎡, and 0.1182n㎡, respectively. This estimation techniques are potentially useful to obtain molecular area for various compounds.

방실차단 및 심실내전도장애에 관한 통계적 고찰

배형준 대한임상병리사협회 1994 대한임상병리사회지 Vol.26 No.1

A statistical study was done on atrioventricular block and intraventricular conduction disturbance in 127 cases taken on 1,761 patients subjected to electrocardiographic examination. The results were as follows 1. Total incidence of conduction disturbance was 7.21%, atrioventricular block was 2.67% and intraventricular block was 4.54% of total patients. Among of these, first degree atrioventricular block was 2.44% which was the most common occurred one, and complete right bundle branch block(2.33%), incomplete right bundle branch block (1.13%), left anterior hemiblock(0.74%), third degree atrioventricular block and left bundle branch block(each 0.17%), bifascicular block(0.11%), second degree atrioventricular block and left posterior hemiblock(erach 0.06%) in the order of frequency. 2. The ratio of male to female was 1.2:1 for atrioventricular block and 1.4:1 for intraventricular block. Atrioventricular block and intraventricular block were both common in over fifth decade. 3. The abnormal ECG findings wjth first degree atrioventricular block were sinus bradycardia(27.3%), left ventricular hypertrophy(21.2%), atrial enlargement(12.2%). In third degree atrioventricular block, the majority(50.0%) showed atrioventricular junctional escape rhythm. In complete right bundle branch block, there were 22.8% of atrial fibrillation and 14.3% of myocardial infarction. In incomplete right bundle branch block, there were 29.4% of both atrial enlargement and atrial fibrillation. In left anterior hemi-block. there was 21.5% of sinus bradycardia.

치과용 고카라트 금합금의 시효경화와 상변태 과정

배동화,설효정,이희경,김형일 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.1

The hardening behavior and phase transformation process of a dental high carat gold alloy by isothermal ageing treatment were investigated by means of hardness testing, X-ray diffraction study and scanning electron microscopic observations. The following results were obtained. The a single phase with a face centered cubic structure was finally transformed into the coexistence of the Ag-rich α₁phase with a face centered cubic structure and the AuCu I' type ordered phase with a face centered tetragonal superlattice structure by ageing of the solution-treated specimen at 400℃ and 500℃. By ageing at 500℃, the metastable Ag-rich α₁' and AuCu I' type ordered phases were formed prior to the final formation of the equilibrium Ag-rich α₁ and AuCu I type ordered phases . The hardening effect was more significant and the overageing with softening was slower by ageing at 400℃ than 500℃. By ageing at 500℃, the hardening was attributed to the formation of the metastable AuCu I' type ordered phases from the a phase, the overageing with softening was due to the formation of the AuCu I type ordered phase from the metastable Ag-rich α₁' and AuCu I' type ordered phases. By ageing at 500℃, the incubation period which limited the beginning of the hardening in the early stage of ageing existed, and the formation and growth of the precipitates which contributed the overageing with softening were observed concurrently at the grain boundaries and within grains. By ageing at 400℃, the hardening was attributed to the formation of the AuCu I type ordered phase from the a phase, the overageing with softening was due to the growth of the grain boundary precipitates of the lamellar structure composed of the Ag-rich α₁and AuCu I type ordered phases into the grain interior.

내용기반 분류에 의한 비디오 검색 시스템 설계

배종식,남윤성,양동일,최형진 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-

We suggest how to search news data by dividing structurely, by classifying meaningly, and by itemizing them by contents. The structural division divides the scene using correlative information between frames such as the distribution of the space brightness and the irregularity of brightness, and time relation etc. The meaning classification means to classify the detailed contents of news video as articles comparing special information extracted frame key frame with previous knowledge information. This study classifies the articles as the anchor's scene and the non-anchor's scene using the character that the progress of news is repeated centering around the anchor frames by periods. We get the non-anchor's scene to search the news data itemizing speech scene, interview scene and general scene, and classifying as articles. Also, we use the function of searching for summary by news icons and search the caption by processing the integration of caption, and design them to be able to search the news video quickly classifying news video as contents and indexing them.