地籍境界 不一致 地域의 境界決定

김희규,김소철,이형복,홍철순 慶南專門大學 1998 論文集 Vol.26 No.1

GaN DFB-레이저다이오드용 회절격자의 제작

김상진,이형규,위명용 충북대학교 컴퓨터정보통신 연구소 2001 컴퓨터정보통신연구 Vol.9 No.1

GaN 에피층을 CCl2F2 와 Ar 가스를 사용한 RIE로 건식각 공정을 개발하여 청색발광용 DFB 레이저 다이오드의 회절격자를 제작하였다. 회절격자의 패턴은 레이저 홀로그래피와 최적조건의 식각 조건 하에서 제작되었다. 얇은 감광제만을 식각 마스크로 사용하면 플라즈마에 의한 마스크 침식에 의해 불규칙적이며 굴곡이 있는 회절격자가 형성되었다. 반면 SiO2/감광제 이중 마스크를 사용하면 마스크 침식 현상이 현저히 감소하여 매우 선명하며 주기적인 격자를 형성할 수 있었다. 제작된 회절격자는 주기 0.25 ㎛, 깊이 약 50nm 인 정현파 형태를 가지고 있었다. We investigated RIE etching parameters of GaN using CCl2F2 and Ar gases and fabricated DFB gratings for the GaN based blue laser diode. Grating patterns were defined by the laser holography followed by RIE etching. Using thin PR as an etch mask, the mask erosion was severe to render the etched pattern rough and aperiodic. However, when SiO2/PR double mask was employed, the mask erosion problem was greatly reduced and a sharp and periodic pattern was obtained. the fabricated grating had a sinusoidal shape with period of 0.25 ㎛ and depth of 50 nm.

韓·日의 도구시스템과 생활시스템의 異同에 관한 연구

홍철순,이형복,김희규,김소철 慶南專門大學 1999 論文集 Vol.27 No.1

Synergistic Fumigation Effect of Ethyl formate and Methyl salicylate against allosobruchus chinensis (L.)

Kashinath Chiluwal,Byung Ho Lee,Kwon Tae Hyung,Junheon Kim3,Soon Do Bae,Yonglin Ren,Chung Gyoo Park 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

Ethyl formate (EF) and methyl salicylate (MS) are naturally occurring and generally safe substances for use. Sub-lethaldoses of EF and MS were evaluated to find synergistic fumigation action against azuki bean beetle (ABB), Callosobruchuschinensis (L.). Susceptibility of the ABB to EF was in the order of eggs =adults >larvae >pupae. Adults was most sensitiveto MS, and followed by eggs. Larvae and pupae were highly tolerant to MS. However, in synergistic effects of EF andMS showed that all the stages of ABB were highly sensitive in the order of adults >eggs >larvae >pupae. Highest synergisticratio was found against adults when using LC10 of both EF and MS as compared to other stages. Notably, MS synergizedthe EF exposure to all stages of ABB. This finding paves the way to exploiting synergistic activity of MS and EF againstABB for the safeguard of stored azuki bean grains.

한국인에서의 CYP2C19 돌연변이 유전자형의 다형성에 관한 연구

채석,채상우,양동규,김형기,권준택,염윤기 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The S-mephenytoin 4'-hydroxylase (CYP2C19) metabolizes a number of clinically used drugs and shows a marked interethnic difference in the incidence of the poor metabolizer (PM). Recent studies have found a third (CYP2C19*4) and forth (CYP2C19*5) mutations in Caucasians. In the present study, we genotyped 536 healthy unrelated Koreans for functionally defective alleles, CYP2C19*2, CYP2C19*3, CYP2C19*4, and CYP2C19*5. Detection of the normal (CYP2C19*1) and defective alleles was performed by polymerase chain reaction/restriction enzyme analysis. The genomic DNA was isolated from peripheral blood. The allelic frequencies of the wild-type (CYP2C19*1) and CYP2C19*2 were 72.6% and 27.4%, and the wild-type (CYP2C19*1) and CYP2C19*3 were 85.1% and 14.9%, respectively. For each CYP2C19*2 and CYP2C19*3 the observed number of three genotypes were similar to those calculated in accordance with the Hardy-Weinberg equation. The frequencies of homozygotes for CYP2C19*2, CYP2C19*3 and compound heterozygotes were 9.7%, 2.8% and 4.2%, respectively. The mutants of CYP2C19 were identified in 36 subjects (16.7%). The mutated CYP2C19*4 and CYP2C19*5 alleles were not detected in this study. These results suggest that frequency of mutants of CYP2C19 in Koreans resembled the Orientals rather than Caucasians, and we founded the racial variability of the frequencies of the CYP2C19*4 and CYP2C19*5 alleles in the Orientals.

Optimization of hexanoic acid production in recombinant <i>Escherichia coli</i> by precise flux rebalancing

Kim, Seong Gyeong,Jang, Sungho,Lim, Jae Hyung,Jeon, Byoung Seung,Kim, Jungyeon,Kim, Kyoung Heon,Sang, Byoung-In,Jung, Gyoo Yeol Elsevier 2018 Bioresource technology Vol.247 No.-

<P><B>Abstract</B></P> <P>The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant <I>Escherichia coli</I> strains. First, a hexanoic acid-producing <I>E. coli</I> strain was constructed by expressing genes encoding β-ketothiolase (BktB) from <I>Cupriavidus necator</I> and acetyl-CoA transferase (ACT) from <I>Megasphaera</I> sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four synthetic 5′-untranslated regions were designed for <I>atoB</I> using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant <I>E. coli</I> strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of <I>atoB</I> is critical for production of hexanoic acid.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recombinant <I>E. coli</I> strains were constructed to produce hexanoic acid. </LI> <LI> The enzyme that catalyzes the last step of the hexanoic acid pathway was replaced. </LI> <LI> Precise flux rebalancing at the acetyl-CoA branch point was achieved. </LI> <LI> The productivity achieved was 8.7-fold higher than that in the parental strain. </LI> <LI> The productivity of the optimized strain was 14.7 mg/L/h. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Case Report : A Case Report of Familial Renal Hypouricemia Confirmed by Genotyping of SLC22A12, and a Literature Review

( Hyung Oh Kim ),( Chun Gyoo Ihm ),( Kyung Hwan Jeong ),( Hyun Joon Kang ),( Jae Min Kim ),( Hyung Suk Lim ),( Jin Sug Kim ),( Tae Won Lee ) 대한전해질학회 2015 Electrolytes & Blood Pressure Vol.13 No.2

A 24-year-old male visited our hospital because of pain in both flanks. His biochemistry profile showed an elevated serum creatinine level and low serum uric acid level. History taking revealed that he had undertaken exercise prior to the acute kidney injury (AKI) event, and he stated that family members had a history of urolithiasis. His renal profile improved after hydration and supportive care during hospitalization. Although the patient was subsequently admitted again due to AKI, his status recovered with similar treatment. Since the diagnosis of the patient was familial renal hypouricemia with exercise-induced AKI, we performed genotyping of SLC22A12, which encodes human urate transporter 1. The diagnosis was confirmed by the detection of a homozygous mutation of W258X. We herein, report a case of familial renal hypouricemia confirmed by genotyping of SLC22A12, and review the relevant literature.

당뇨병 환자에서 99mTc-DMSA SPECT를 이용한 기능적 신용적의 측정

김태형,홍성표,김명재,이태원,양형인,김덕윤,임천규 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.3

About 40% of patients with diabetes mellitus develops to diabetic nephropathy, and these patients show increment of glomerular filtration rate and renal volume at early phase. 99mTc-DMSA SPECT (single photon emission computed tomography) can measure a functional renal volume because 99mTc- DMSA it is taken up by the viable proximal tubular cells located in the renal cortex. To evaluate the renal volume in early diabetic nephropathy, we compared functional renal volume between diabetic patients and control, renal transplantation donor. 99mTc-DMSA was injected intravenously and SPECT was done after 2 hours in 15 diabetic patients, 18 renal transplantation donors, and 2 patients with end stage renal disease due to chronic glomerulonephritis. In diabetic patients with creatinine clearance more than 30ml/min and proteinuria, right and left renal volume were 247±22ml/BSA(m2), 256±37ml/BSA (m2), which were greater than those of diabetic patients with normal renal function and without proteinuria, or renal transplaation donors(P$lt;0.05). In conclusion, we measured the functional renal volume by 99mTc-DMSA SPECT. Renal volume of diabetic patients with proteinuria were larger than the volume of control group or diabetic patients with normal renal function and without proteinuria. 99mTc-DMSA SPECT was thought to be useful test a for the measurement of functioning renal volume.

Poster Session:PS 0545 ; Nephrology : A Case Report of Capd Peritonitis with the Pathogen of O. Anthropi

( Hyung Oh Kim ),( Chun Gyoo Ihm ),( Tae Won Lee ),( Kyung Hwan Jeong ),( Seul Ki Kim ),( Ji Yun Park ),( Jin Sug Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: We report a rare case of CAPD peritonitis caused by Ochrobacterium anthropi. Introductions: Among the continuous ambulatory peritoneal dialysis(CAPD) patients, peritonitis is one of the most commonly taken complications, and also the general cause of dialytic modality exchange to hemodialysis. Usual pathogens of CAPD peritonitis may be bacteria, fungi, mycobacteria. Coagulase negative staphylococci, S. aureus, campylobacter, pseudomonas are common reported pathogens among the bacteria, of CAPD peritonitis cases, meanwhile candida is among fungi. Ochrobacterium anthropi is one species of Brucellaceae, which is rare pathogen of human disease. Case (Methods and results): 73 years old female, who was on CAPD due to diabetic end stage renal disease visited Kyung-Hee University hospital with intermittent abdominal pain. Body fi uid analysis showed increased white blood cell(WBC) count of 26,750/mm3 with her peritoneal fi uid. Culture study with peritoneal fi uid suggested O. anthropi, and DNA sequencing with PCR was consistent with O. anthropi. Intraperitoneal ceftazidime and cefazolin were administrated as empirical antibiotics. Ceftazidime resistance was noted with the result of antibiotics sensitivity test at 7th day of hospitalization, and antibiotics were changed into intraperitoneal gentamicin, which showed sensitivity to the pathogen. CAPD catheter removal and antibiotics re-exchange into imipenem and cefazolin, which were other sensitive antibiotics by the sensitivity test, were done since clinical manifestation and peritoneal fi uid WBC count was repeatedly improved and aggravated. The patient discharged with improved lab test results and resolving clinical symptoms afterward. Conclusions: We presentators report rare case of CAPD peritonitis with pathogen of O. anthropi. The pathogen of the case confi rmed by classical microbiologic, and molecular biologic Methods: The patient was unable to treat only with antibiotics, thus CAPD catheter, which might be act as colonizing source, was removed, and the disease resolved.

Test Observations of 14m Radio Telescope(I)

Se-Hyung Cho,Byung-Ryul Auh,Jae-Hoon Jung,Chang-Hoon Lee,Hyun-Goo Kim,Yong-Sun Park,Duk-Gyoo Roh,In-Sung Yim,Bong-Gyu Kim,Tu-Hwan Kim 한국천문학회 1987 天文學會報 Vol.12 No.-