구개열 아동 언어의 진단 평가

신효근,고승오,홍기환,서정환,고도흥,김현기 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1

근적외분광분석법을 이용한 참기름 정량 분석 및 진위 판별

서은정,백주현,우영아,김효진 동덕여자대학교 종합약학연구소 2002 동덕약학연구지 Vol.6 No.-

In this study, sesame oil have been analyzed by near infrared spectroscopy (NIRS). Sesame oil was mixed with the other edible oil. NIR transmittance spectra of sesame oil mixtures were acquired by using a dip probe. Partial least squares regression (PLSR) was applied to develop a calibration model over the spectral range 1100-1750nm. The calibration model predicted the content of sesame oil for validation set with a standard errors of prediction (SEP) of 0.3871%. Soft Independent Modeling of Class Analogy (SIMCA) was used to identify sesame oil and adulteration. This model identified the sesame oil and adulteration for validation set with 100% accuracy. This study showed that the rapid and non-destructive the determination of the quantitative analysis of sesame oil, the identification of sesame oil and adulteration was successfully performed by portable NIR system.

제지폐수 처리용 미생물 제제 개발

서현효,김형갑 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

This study presents the effects of microbial augmentation on treatment of paper mill wastewater. A strain SJ7 which produces an extracellular cellulase was isolated from samples of sawdust and soil. Bases on the morphological and physiological characteristics, the isolate SJ7 was identified as Pseudomonas sp. and named Pseudomonas sp. SJ7. Microbial augmentation CE-1 is a special blend of bacterial cultures(Nasseria sp. S4, Bacillus sp. JB2, Corynebacterium murium BY9, Pseudomonas sp. SJ7) selected and adapted to improve their ability to treat paper mill wastewater. For the evaluation of industrial applicability of microbial augmentation CE-1, treatment efficiency was examined using paper mill wastewater. The reducing rate of chemical oxygen demand(COD) and biochemical oxygen demand(BOD) by CE-1 against paper mill wastewater was 84.5% and 91.8%, respectively.

원유 분해균주의 분리 및 특성

서현효,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

Bacterial strains which degrade crude oil were isolated by liquid culture from oil-spilled soil, and four isolates were selected among them. The strain H17-1 was finally selected after testing emulsifying activity and oil conversion rate. The strain H17-1 was identified as a Norcardia sp. based on the test for morphological, biochemical and physiological characteristics. It appears to be highly specialized for growth on crude oil in minimal salts medium since it showed preference for oil or degradation products as substrates for growth. It was found that it could grow on at least fifteen different hydrocarbons. The optimum cultural and environmental conditions were seeked. Cell growth and emulsification activity as a function of time were also determined. Crude oil degradation and the reduction of product peak was identified by the analysis of remnant oil by gas chromatography after 3 days of cultivation. Approximately 83% of oil were converted into a form no longer extractable by organic solvents.

미생물 유래 β-glucan 분리 · 정제 및 이를 이용한 오염물질 정화용 복합 미생물제재 개발

서현효,이현돈,김형갑 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14

β-glucan occuring mainly in mushrooms and fungi are attractive candidates for numerous industrial application due to their pharmacological activity such as antitumor activity via immunomodulating behavior and excellent rheological properties such as emulsification, viscosity, jellation, etc. Screening of β-glucan producing microorganism was performed by congo red dye binding assay of ethanol-precipitates from each culture filtrate. The β-glucan produced form the selected strains was purified mainly by directly diluting the culture broth with the same volume of water, removal of cells by centrifugation at 4,000×g for 20 mim, and precipitation with 3 volumes of ethanol. The monosaccharide composition was analyzed by either TLC or HPLC afer trifuoroacetic acid hydrolysis. The molecular mass of the β-glucan of HH-71 strain was estimated to be about 4,500,000 dalton on a GFC-HPLC chromatogram. A Bacillus protease coated with β-glucan showed 3-times more increased thermal stability comparing to the uncoated control when stored at 30℃ for 1 week. In a similar manner coating of wastewater (containing chlorinated organic compounds) treatment bacteria with β-glucan resulted in significant increase of thermal stability. Although more detailed experimental data need to be accumulated, these preliminary data clearly suggest that β-glucan is applicable as a coating material to stabilize activity or viability of industrial enzymes and microorganisms for treatment of wastewater. Based on these results, β-glucans obtained from HH-71 and HH-87 strains are currently investigated for coating ablility of microbial consortia for the treatment of wastewater.

Benzene 분해 미생물의 분리 및 특성

서현호,정병운,전기일,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

Flavobacterium sp. BEN2 was selected and identified out of 64 benzene-degrading bacteria isolated from soils and waste waters by the enrichment culture technique. The optimal temperature, pH, and benzene concentration for the growth of Flavobacterium sp. BEN2 appeared to be 30℃, 7.0 and 2000 ppm, respectively. Cell growth almost revealed a stationary phase at 24 hours after cultivation and benzen was completely degraded during as benzen, benzoic acid, phenol, p-cresol, salicylic acid, catechol, gentistic acid and toluene, but did not grow ob o-cresol, m-cresol, p-xylene and p-toluic acid. With this strain, catechol was degraded through both meta- and ortho- cleavage pathway. Therefore, it is suggested that Flavobacterium sp. BEN2 could be effectively used for the biological treatment of wastewater containing benzene in the presence of some aromatic compounds and organic solvents.

Bacillus sp. SY12을 이용한 생물응집체 생산

서현효,정병윤,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

A gram(+) bacteria that produced microbial flocculant was isolated from soil and classified as a Bacillus species and named as Bacillus sp. SY12. The culture conditions of the strain for flocculant production were studied in a shake flask. Optimum temperature and initial pH for flocculant production were 30℃ and 6.5, respectively. The optimized medium has following composition : glucose 20 g/ℓ, NH_4NO_3 0.5 g/ℓ, K_2HPO_4 1.0 g/ℓ, KH_2PO_4 0.8 g/ℓ, MgSO_4·7H_2O 0.5 g/ℓ, MnSO_4·4H_2O 0.3 g/ℓ, CaCO_3 0.5 g/ℓ, yeast extract 0.3 g/ℓ in tap water. Under the optimum culture conditions, flocculant production was improved by ten times in comparison with in basal medium. In the jar fermentor, the highest flocculating activity was obtained at 30-h cultivation time and the flocculant was increased with the increase of cells.

뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

정수현,서형주,김윤숙,이효구,강덕호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2

기존의 방법을 일부 수정하여 뇨중 3-methylhistidine을 분석하였다. 뇨중 3-methylhistidine을 fluorescamine 유도체화하여 HPLC에 주입하고 C_18 column과 10 mM acetonitrile/sodium phosphate buffer(pH 7.5)로 분리·용출시켜 형광검출기로 측정하였다. 3-methylhistidine의 체류시간은 7분 이내이었으며, histidine과의 분리상태도 서로 간섭함이 없이 양호하였다. 뇨에 3-methylhistidine을 첨가하고 이를 분석하였을 때의 회수율은 93∼106%로 높은 수준이었다. 체육학과 남학생중 웨이트 트레이닝 단련자와 비단련자를 대상으로 조사한 단기간의 웨이트 트레이닝에 따른 뇨중 3-methylhistidine 함량의 변화는 두 집단 모두 웨이트 트레이닝후의 3-methylhistidine 분비량이 유의하게 증가하였다. A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 mM sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhistidine from urine control were 93% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary 3-methylhistidine content of volunteers increased significantly after weight training.

Bacillus sp. SK31에 의한 생물흡착제의 분리 및 생산

김형갑,서현효 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.1 No.-

A strain (designated SK31) which produces an excellent adsorption substance was isolated from soil samples and identified as Bacillus species. The major adsorption substance (biosorbent SK31) produced by Bacillus sp. SK31 was purified by ethanol precipitation and cetylpyridinium (CPC) precipitation. The adsorption charactics of zinc and lead ions on bioadsorbent SK31 were investigated. The equilibrium isotherms showed that bioadsorbent SK31 took up zinc and lead from aqueous solutions to the extent of about 52㎎/g and 112㎎/g, respectively. The culture conditions at the flask level of Bacillus sp. SK31 were investigated for the production of polysaccharide bioadsorbent, SK31. The optimum pH and temperature for sorbent production were 7.5 and 30℃, respectively. The important substrates for sorbent formation were glucose and ammonium nitrate. In the optimized medium, sorbent production was improved three folds in comparison with the basal medium. In the jar fermenter, the highest sorbent production was obtained at 60 h cultivation time and the amount of biosorbent SK31 at that time was 8.2g/1.

고등학교의 환경과학교육이 환경의식과 태도에 미치는 영향

김형갑,서현호,조경환 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

In order to investigate the effects of environmental scientific education on high school students' environmental knowledge, behavior, and practice, this study selected the test group of two schools that enforce environmental scientific education and the control group of two schools that do not enforce it. These are the results of the comparison between two groups. 1. The comparison of the average mark on the environmental knowledge, behavior, practice between two groups showed following results. In regard to the knowledge, the mark of the test group was higher than that of the control group. (p<0.001) and as for the behavior, the point of the test group was also higher than the control group(p<0.05). With respect to the practice, two groups made little difference. 2. The factor that had most greatest influence on the environmental knowledge was the students' school achievements(R2=15.0%), and in turn, the environmental instructions and the degree of consciousness on the necessity of environmental preservation were the important factors. In regard to the behavior, the degree of consciousness on the necessity of environmental preservation was the most important factor(22.1% of reliability), and in turn, the degree of concern on the environmental problems, the school achievements, and the proportions of parents' environmental preservation practice were important. The coefficient of reliability was 29.1%. As for the practice, the most far-reaching factor was the extent of the students' concern on the environmental problems(19.3% of reliability). and the proportions of parents' environmental preservation practice, the degree of the consciousness on the necessity of environmental preservation, the level of the economic conditions, the extent of parents' concern on the environmental problems were important in turn. The coefficient of reliability on the practice were 30.2%.