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Kwon, Hyukjae,Yang, Hyerim,Lee, Seongdo,Nilojan, Jehanathan,Bathige, S.D.N.K.,Nam, Bo-Hye,Wan, Qiang,Lee, Jehee Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.74 No.-
<P><B>Abstract</B></P> <P>Kazal-type serine protease inhibitors (KSPIs) play important roles in the regulation of endogenous proteases, cell development, blood coagulation, and immune response. In this study, we identified and characterized a KSPI homologue (<I>SsKSPI</I>) in black rockfish, <I>Sebastes schlegelii</I>. The full-length cDNA sequence of <I>SsKSPI</I> was 532 base pairs (bp), including an open reading frame (ORF) of 330 bp, which encodes a polypeptide of 110 amino acids with a signal peptide of 21 amino acids. The greatest value for identity (42.9%) and similarity (50.9%) was observed with <I>Channa striata</I> KSPI. We purified the recombinant protein of <I>SsKSPI</I> and performed protease inhibitory assays using three common serine proteases. The recombinant SsKSPI exhibited specific inhibitory activity against subtilisin A in a dose-dependent manner. Tissue distribution of <I>SsKSPI</I> mRNA has been examined amongst 10 important tissues in healthy rockfish and the liver was found to be the predominant expression organ of <I>SsKSPI.</I> The modulation of <I>SsKSPI</I> expression under immune challenges was also investigated in the liver. The <I>SsKSPI</I> mRNA expression was significantly up-regulated in response to both bacterial (<I>Streptococcus iniae</I> and lipopolysaccharide) and viral (polyinosinic:polycytidylic acid) challenges. Overall, we propose that <I>SsKSPI</I> is potentially involved in the hepatic immune response against bacterial and viral infections in black rockfish.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A KSPI homologue (<I>SsKSPI</I>) was identified in <I>Sebastes schlegelii.</I> </LI> <LI> The recombinant SsKSPI specifically inhibited subtilisin A activity. </LI> <LI> The highest constitutive expression of <I>SsKSPI</I> was detected in the liver. </LI> <LI> <I>SsKSPI</I> gene expression was significantly inducible upon bacterial and viral challenges. </LI> <LI> <I>SsKSPI</I> is potentially involved in the hepatic immune response in black rockfish. </LI> </UL> </P>
권혁재(Hyukjae Kwon),공병석(Byungseok Kong),이종용(Jongyong Lee) 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
Structure design of crash pad module to avoid knee modifier is must for acquiring EURO NCAP 5★. In order to remove Knee Modifier (Variable Contact Modifier and Concentrated Loading Modifier), there must not be steel part without padding in the knee inspection zone. In this thesis, the new concept of crash pad for no knee modifier was proposed, and CAE was performed for structural optimization. This new crash pad structure can bring out the problems (the head impact failure, static load failure, idle resonance) by lower stiffness of crash pad structure by the dropping of material strength. In order to prevent these problems and to account for these phenomena from the early design stages, optimal Crash Pad structure was proposed and developed using CAE simulating tool.
손한창,Hyukjae Kwon,Seongdo Lee,Qiang Wan,이제희 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.10
Scuticociliatosis, caused by the parasitic pathogen Miamiensis avidus, poses a significant threat to olive flounder farms in South Korea. Infected fish suffer from severe systemic infections affecting various organs, with potential secondary bacterial diseases. This study investigated the emergence of different M. avidus serotypes in 20 olive flounder farms on Jeju island, South Korea, from 2015 to 2020. Additionally, we identified Tenacibaculum maritimum as a co-infecting bacteria. Based on serotyping and monitoring data, we developed a trivalent vaccine targeting two serotypes of M. avidus and one strain of T. maritimum. The efficacy of the vaccine was evaluated under laboratory conditions and demonstrated a relative percentage of survival (RPS) of 75%, 80%, and 93% for M. avidus serotype I, M. avidus serotype II and T. maritimum, respectively. Furthermore, successful field trials conducted on four different olive flounder farms resulted in significantly higher survival rates (52%–76% RPS) and weight gains in vaccinated fish. Overall, this study presents an effective vaccine against M. avidus and T. maritimum infections in farmed olive flounder, making a valuable contribution to sustainable aquaculture in South Korea.