Identification and characterization of Dunaliella salina OH214 strain newly isolated from a saltpan in Korea

Minjae, Kim,Hyeon Jun, Oh,Khanh, Nguyen,EonSeon, Jin The Korean Society of Phycology 2022 ALGAE Vol.37 No.4

Carotenoids are effective antioxidants that are found in various photosynthetic organisms. Marine microalgae are an advantageous bioresource for carotenoid production because they do not compete with other crops for freshwater and arable land. This study reports a newly isolated Dunaliella strain from the Geumhong Saltpan on Yeongjong Island, West Sea, Korea. The new strain was isolated and classified as Dunaliella salina through phylogenetic analysis and was named the OH214 strain (Deposit ID: KCTC14434BP). The newly isolated strain can survive in a wide range of NaCl concentrations (0.3-5.0 M NaCl), but grows well in 0.6 to 1.5 M NaCl culture medium. Under high-light conditions (500 ± 10 μmol photons m^-2 s^-1), the cells accumulated three times more β-carotene than under low-light conditions (50 ± 5 μmol photons m^-2 s^-1). The cells accumulated 2.5-fold more β-carotene under nitrogen-deficient (1 mM KNO₃) conditions (3.24 ± 0.36 ㎍ 10⁶ cells^-1) than in nitrogen-sufficient conditions (>5 mM KNO₃). The lutein content under nitrogen-deficient conditions (1.73 ± 0.09 ㎍ 10⁶ cells^-1) was more than 24% higher than that under nitrogen-sufficient conditions. Under the optimized culture condition for carotenoid induction using natural seawater, D. salina OH214 strain produced 7.97 ± 0.09 mg g DCW^-1 of β-carotene and 4.65 ± 0.18 mg g DCW^-1 of lutein, respectively. We propose that this new microalga is a promising strain for the simultaneous production of β-carotene and lutein.

Controlled Crystal Growth of Two-Dimensional Layered Nanomaterials in Hydrogel via a Modified Electrical Double Migration Method

Gwak, Gyeong-Hyeon,Kwon, Na Kyung,Lee, Won-Jae,Paek, Seung-Min,Kim, So Youn,Oh, Jae-Min The American Chemical Society 2017 CRYSTAL GROWTH AND DESIGN Vol.17 No.12

<P>Layered double hydroxide (LDH) nanomaterials of uniform size and homogeneous distribution are successfully developed in agarose hydrogel through the electrical double migration method. To grow LDH crystals in agarose matrix, both cationic (Ni<SUP>2+</SUP>, Ga<SUP>3+</SUP>) and anionic precursors (OH<SUP>–</SUP> and CO<SUB>3</SUB><SUP>2–</SUP>) of LDH are simultaneously transported from solution to agarose hydrogel through electric potential, resulting in the in situ crystal growth of LDH. X-ray diffraction patterns, X-ray absorption spectra, and high-resolution transmission electron microscopic images confirm that the developed LDH is ∼15 nm in size and had a well-defined crystal structure. Scanning electron microscopy and small-angle X-ray scattering spectroscopy showed homogeneous arrays of LDH nanocrystals along agarose sheets with homogeneous distribution. On the basis of the characterization results, we suggest that crystal nuclei are first developed on the agarose chain, and then LDHs homogeneously grow along sheets of agarose matrix.</P><P>Layered double hydroxides (LDHs) of uniform size and homogeneous distribution are developed in agarose hydrogel through the electrical double migration method. Developed LDH had a well-defined crystal structure and homogeneous arrays along agarose sheets with homogeneous distribution. Thus, we suggest that crystal nuclei are first developed on the agarose chain, and then LDHs homogeneously grow along sheets of agarose matrix.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cgdefu/2017/cgdefu.2017.17.issue-12/acs.cgd.7b01252/production/images/medium/cg-2017-01252b_0009.gif'></P>

Electrophoretic Preparation of an Organic–Inorganic Hybrid of Layered Metal Hydroxide and Hydrogel for a Potential Drug‐Delivery System

Gwak, Gyeong‐,Hyeon,Paek, Seung‐,Min,Oh, Jae‐,Min Wiley-VCH 2012 European journal of inorganic chemistry Vol. No.

<P><B>Abstract</B></P><P>We have prepared organic–inorganic hybrid materials consisting of layered metal hydroxides and hydrogel polymers for potential use as a drug delivery system. The hybrid materials were synthesized by an electrophoretic method; anionic precursors, OH<SUP>–</SUP>/CO<SUB>3</SUB><SUP>2–</SUP>, and cationic metal Zn<SUP>2+</SUP> were migrated into hydrogel by applying an electrical potential of 25 V for 20 min to form inorganic nanocrystals inside the hydrogel matrix. Hydrozincite [Zn<SUB>5</SUB>(OH)<SUB>8</SUB>(CO<SUB>3</SUB>)] nanoparticles, a kind of layered metal hydroxide, were determined to develop inside the hydrogel according to powder X‐ray diffraction, FTIR, and X‐ray absorption spectroscopy. Scanning electron microscopic images showed that the prepared hybrid materials have well‐dispersed inorganic nanoparticles in the hydrogel matrix. In order to evaluate the potential of the thus prepared hybrid materials as a drug‐delivery system, the well‐known antioxidant ferulic acid was incorporated and their time‐dependent release profile was obtained by HPLC.</P>

수산화칼슘 처리된 Porphyromonas endodontalis Lipopolysaccharide가 다형핵백혈구의 IL-1과 TNF-α 생성에 미치는 영향에 관한 연구

박찬제,박동성,유현미,오태석,임성삼 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines , namely, interleukin 1 and tumor necrosis factor-α from immune cells, Although monocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-α. Calcium hydroxide has been shown to be an effective medicament in root canal infectionsm, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)_2 may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromanas endodonatalis LPS with calcium hydroxide alters its biological action as neasured by human PMN secretion of IL-1 and TNF-α, and it was compared with Escherichia coli LPS. P.endodonatalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified . 100 ㎍/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)_2 at 37℃ for 7 days. The supernatants were subjected to ultrafiltration. And the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4 × 10^6 cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10㎍/ml) for 24 hours at 37℃ in 5% CO_2 incubator. The supernatants of cells were collected and the levels of IL-1α, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. The results were as follows; 1. The levels of IL-1α, IL-1β, TNF-α from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05). while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05). 3. The levels of secretion or all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with P.endodontalis LPS were significantly lower than those released from PMN stimulated with E. coli LPS (p<0.05).

pH, 이온종류 및 농도, 퇴적물의 성분에 따른 하천 내 불소의 거동특성

이동민(Dong Min LEE),주광진(Kwang Jin Joo),최이송(ISong Choi),장광현(Kwang Hyeon Chang),오종민(Jong Min Oh) 응용생태공학회 2018 Ecology and resilient infrastructure Vol.5 No.1

하천에 유입된 오염물질은 다양한 환경 조건에 의하여 수체 및 퇴적물의 상호작용에 따라 수체의 수질에 영향을 미친다. 특히 최근 수체의 수리학적 체류시간이 상대적으로 긴 정체수역 내에서는 퇴적물의 축적량이 증가하고 있어 퇴적물과 수체 내에서의 물질적 거동특성 파악은 중요한 수질적 관점 요소이다. 본 연구에서는 하천 내 pH, 이온종류 및 농도, 퇴적물의 점토함량에 따라 불소의 거동이 어떻게 변화하는지 검토하기 위하여 경기도에 위치한 중소하천을 대상으로 조건별 실험을 실시하였다. pH에 따른 흡착 및 용출특성 실험결과, pH가 높을수록 불소의 흡착량이 감소하였고, 용출되는 불소량은 증가하는 경향을 나타냈다. 이온농도 및 종류에 따른 흡착 및 용출특성 실험결과, Cl<SUP>-</SUP>, SO₄<SUP>2-</SUP> 이온은 불소의 흡착능에 큰 영향을 주지 않는 것으로 나타났으나, 불소와의 활발한 이온경쟁효과를 지닌 OH<SUP>-</SUP>와의 반응에서는 흡착량이 줄고, 용출량이 늘었음을 알 수 있었다. 퇴적물의 성분에 따른 흡착 및 용출특성 실험 결과 상대적으로 입경이 작은 Silt와 Clay 성분이 많이 포함된 시료에서 불소의 흡착량이 많고 용출되는 불소량이 줄어드는 것을 알 수 있었다. 이는 수체의 환경조건이 퇴적물 내 불소의 흡착 및 용출에 크게 영향을 미치기 때문에, 퇴적물 내 불소를 적절하게 관리하기 위해서는 수체의 환경조건에 대한 이해가 선행되어야 한다는 것을 의미한다. Water quality is affected by the pollutants flowing into rivers since the interaction between water bodies and sediments in various environmental conditions. Especially, accumulation of sediments increases in the stagnant water areas due to a relative long hydrological retention time in the water bodies. Therefore, it is an important factor of water quality to understand characterization of the material behavior in water bodies and sediments. In this study, the objective of the conditional experiments was small and medium sized streams located in Gyeonggi-do. To estimate how the changes of fluoride behavior, depending on the pH, ion type, concentration, and clay contents. The pH results showed a trend that adsorption amount of fluorine decreased and the dissolution of fluorine increased following by pH increasing. The concentration and type of ions results showed that Cl<SUP>-</SUP> and SO₄<SUP>2-</SUP> ions had no significant effect on the adsorption ability of fluorine, the amount of dissolution was increased because OH<SUP>-</SUP> ion had active competition with fluorine in the reaction. The ingredient of sediment results showed that the amounts of fluoride adsorption and dissolution were reduced in samples, which contain relatively large amounts of Silt and Clay components. This means that the environmental conditions of water bodies greatly affect the adsorption and dissolution of fluoride in the sediments, so that proper management of fluoride in the sediments must precede an understanding of the environmental conditions of the water bodies.

주파수의존 근관장 측정기가 습윤한 근관내에서 근첨을 인지하게 되는 원리에 관한 연구

오태석,유현미,박한수 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.2

Gibberellin 處理가 파인애플 果實肥大에 미치는 影響

吳現道,吳迎碩 濟州大學校 亞熱帶農業硏究所 1994 亞熱帶農業硏究 Vol.11 No.-

Pineapple은 施設栽培時 生長調節劑 Gibberellin의 效果를 究明하기 위하여 1992年 7月 3日에 Gibberellin (25, 50, 100, 200, 400ppm)을 처리하였을 경우 파인애플 과실비대에 미치는 효과를 요약하면 다음과 같다. 1. 葉長, 葉數, 抽苔期, 成熟期는 處理間에 有意性이 없었다. 2. 冠芽크기, 果梗長, 果徑, 果重은 處理間에 有意性이 認定되었다. 3. 果重에 있어서는 無處理區에 비해 25, 50, 100, 200ppm까지는 濃度가 높아짐에 따라 果重이 增加하였으나 400ppm 處理區에서는 200ppm 處理區보다 減少하여 200ppm 處理區에서 가장 良好한 結果를 보였다. 4. 果長과 葉長間에는 正의 相關關係를 보였고, 果重과 果徑間에는 高度의 相關關係가 認定되었으나 기타 형질에 있어서는 相關程度가 낮은 편이었다. This experiment was carried out in order to investigate the effects of gibberellin (25, 50, 100, 200, 400 ppm) treatment on growth of furit in Pineapple. Gibberellin was applied on july 3, 1992 to fruit 1. There were not significant differences among treatments for the length of leaves, number of leaves per plant, days to inflorescences emergence and days to maturity. 2. There were significant differences among treatments for crown size, peduncle length, furit diameter and fruit weigtht. 3. Fruit weigth increaseed as the concentration of gibberellin increased form 0 to 200ppm and decreased at 400ppm compared to 200ppm. 4. Fruit length was positively correlated with length of leaves and fruit weight was positively correlated with fruit diameter. However, there were no significant correlated among the other characters.

Stevia에 있어서 優良系統 選拔을 爲한 主要特性 分離에 관한 硏究

吳現道 제주대학교 1980 논문집 Vol.12 No.-

This study was conducted to discover some main characteristics for the selection of a superior line in Stevia rebaudiana Bertoni. The results obtained are summarized as follows: The medium-sized leaf appears far more frequently than the large and small-sized leaf, and the Latter two appear in some what similar ratio to each other. Shape of the leaf is most frequently elliptic, and orbicular and linear shapes are rather infrequent. Stems of medium length appear most frequently, and shorter stem infrequently. The length of internode occurs in a positive correlation with the length of stem. The branch develops more commonly at an acute angle. The plant with a minimum of branches appears with maximal frequency. Those plants which have major and medium number of branches appear in similar ratio to each other. The maojrity of plants begin to flower in early September.

스테비아 種子의 發芽性에 關한 硏究

吳現道,金龍湖 제주대학교 1983 논문집 Vol.16 No.-

스테비아에 있어서 明暗條件下에서 溫度別, 播種樣式別 발아의 效果를 究明하기 爲하여 시험을 逐行하였다. 結果를 要約하면 다음과 같다. 發芽狀態는 暗條件에서는 全般的으로 明條件에 比해서 低調하였으며 不量苗率이 높고, 發芽의 徵候만 나타내는 것이 많았으며 그 程度는 15℃ 低溫인 條件이 20℃ 및 25℃ 條件에 比해서 더욱 많았다. 發芽溫度는 15℃, 低溫보다 20˚∼25℃에서 效果가 좋았고 15℃∼25℃ 變溫條件은 20℃∼30℃ 變溫條件보다 發芽率 및 發芽勢가 優勢하였다. 明條件下에서 發芽期間이 短縮되고 發芽率 및 發芽勢가 優勢하게 나타났으며 播種方法은 이끼 被覆이 가장 效果的이었고 地表播, 半覆土播, 覆土播의 順位를 보였다. This experiment was conducted to investigate the effect of germination at different temperature and seeding method under light and dark condition in Stevia (Stevia rebaudiana Bertoni) The result are summarized as follows; Percentage and speed of germination, and normal seedling were greater under light condition than under dark. Percentage and speed of germination, number of normal seedlings increased as temperature increased from 15 to 20 or 25℃. Alternating temperature from 15 to 25℃ was more effective for germination than 20 to 30℃. Molding with moss was the most effective for germination under both light and dark conditions. No molding was comparable with 50% molding but molding with soil resulted in poor germination.

패션쇼 연출기획의 사례분석연구

오현남,김현주 대한가정학회 2003 Human Ecology Research(HER) Vol.41 No.11

The purpose of this study is : first. to examine factors of fashion show planning and production and to classify types of fashion show planning and production: second, to compare and analyze the factors through a case analysis to defined their differences. For the purpose, data are collected to compare and analyze from literature as well as the interview with fashion show planning and production companies in Korea. The result shows that all the factors are considered on a practical level except security and publicity among 15 factors presented in literature : the purpose of the show, target spectators, product selection, product prices, type of show, size, location, models, time of show, duration, theme, security, budget, and publicity. Show types are divided into national brands, designer brands, imported brands, and collections. The result of the comparison and analysis of the factors of the planning and production by type shows that factors vary greatly depending on the purpose of the show, target spectators, and form of the show and products. By analyzing the case study on fashion show planning and production on a practical level, the study recognizing the need of specialized development of fashion show planning in Korea s fashion industry, is a basic process of theory systematization for the planning and production in its marketing aspects, and will provide relevant theories in such aspects for those who want to enter into the field.