[P8-247] Isolation of flavonoids from the root of Glycyrrhiza uralensis Fischer through GABA<SUB>A</SUB>-BZD receptor activity-guided fractionation

Hyeim Yong,Dongsoo Kim,Jihae Park,Minseok Yoon,Hyejin Yang,Suengmock Cho,Namin Baek 한국식품영양과학회 2010 한국식품영양과학회 학술대회발표집 Vol.2010 No.10

COVID-19가 건강관련 삶의 질에 미치는 영향

이혜임 ( Hyeim Lee ),강상조 ( Sang-jo Kang ) 한국체육학회 2021 한국체육학회지 Vol.60 No.3

이 연구는 세계적으로 유행하고 있는 COVID-19가 인간의 건강관련 삶의 질(Health-related quality of life: HRQOL)에 미치는 영향을 조사하는 데 목적이 있다. 이러한 목적을 달성하기 위해 18세 이상의 한국인 437명을 대상으로 두 개의 건강관련 요인(신체적, 정신적)과 이를 측정하는 8개 문항으로 구성된 ‘건강관련 삶의 질’ 척도(SF-8 scale)을 이용하여 COVID-19 전과 후에 그들이 지각하고 있는 건강관련 삶의 질 수준을 Online 방법을 이용하여 조사하였다. 이 연구에서 구명하고자 한 연구문제는 첫째, COVID-19가 발생하기 전, 후 간 HRQOL에 차이가 있는가? 둘째, 만일 전, 후 간에 차이가 있다면, 그러한 변화는 성별과 연령수준에 따라 차이가 있는가? 이다. 수집된 자료는 연구문제에 따라 t 검증방법, 반복측정 ANOVA, 효과크기, Pearson의 r을 적용하여 분석, 검증하였다. 분석결과 COVID-19 발생 전과 후, 응답자들이 지각한 전체적인 HRQOL의 평균은 4.42에서 3.61로 낮아졌다. 신체적, 정신적 건강요인과 SF-8의 모든 영역에서 COVID-19 전보다 후에 현저하게 낮아졌다. 특히 정신적 건강요인에 해당하는 사회적 기능(SF; M = -1.41)과 정신건강(MH; M = -1.23)영역에서 가장 크게 부정적으로 변화하였다. 응답자들이 지각한 COVID-19 발생 전과 후 전반적 HRQOL의 변화 정도는 남녀와 연령수준에 따라 차이가 있는 것으로 나타났다. 남자보다는 여자(M = -0.99), 그리고 31-50세의 중년집단(M = -0.91)에서 가장 크게 감소하였다. 이러한 HRQOL의 부정적인 변화에도 불구하고 척도의 중간점인 3.0보다 낮지 않다는 사실은 응답자들은 COVID-19을 매우 심각하게 받아들이지 않고 있다는 것이다. The purpose of the study is to investigate and analyze the change in health-related quality of life in Korean residents by comparing before and after COVID-19 outbreak. A total of 437 individuals (aged ≥18 years, Korean nationality) participated in this study, and all respondents completed the questionnaire by self-administrative method between June 15 and 30, 2020 in Korea. Health Related Quality of Life (HRQOL) was measured using the Short Form 8-item (SF-8) which involves both physical (PCS) and mental (MCS) health components, and general demographics including age and gender were collected. The descriptive statistics, including mean and standard deviation, were calculated for the subgroups and the entire sample. Paired t-tests and the Repeated-measure ANOVA, Cohen’s effect size (d and partial η²), and Pearson’s correlation (r) were used to analyze the mean differences and associations between before and after COVID-19 outbreak for SF-8 items and PCS and MCS domains. The result demonstrated a significant difference in overall SF-8 scores with large effect size between before and after the outbreak. Both PCS and MCS scores were significantly lower after COVID-19 outbreak. The mean change for MCS was greater than PCS (p < .001). The change in overall HRQOL before and after COVID-19 occurrence differed according to gender and age level. The mean change scores were greater for the female (M = -0.95) and for the age 31 - 50 group (M = -0.91). The study suggested that COVID-19 has negatively affected HRQOL, and the impact differs within age and gender groups. Understanding the influence of COVID-19 on both physiological and psychological health is essential to effectively address the issue and improve the future healthcare system.

프로그래밍 가능한 데이터 평면의 가상화를 통한 이기종 IoT 프로토콜 연동 지원에 관한 연구

양혜임(Hyeim Yang),한솔(Sol Han),백상헌(Sangheon Pack) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.2

The Construction of Regulatory Network for Insulin-Mediated Genes by Integrating Methods Based on Transcription Factor Binding Motifs and Gene Expression Variations

Jung, Hyeim,Han, Seonggyun,Kim, Sangsoo Korea Genome Organization 2015 Genomics & informatics Vol.13 No.3

Type 2 diabetes mellitus is a complex metabolic disorder associated with multiple genetic, developmental and environmental factors. The recent advances in gene expression microarray technologies as well as network-based analysis methodologies provide groundbreaking opportunities to study type 2 diabetes mellitus. In the present study, we used previously published gene expression microarray datasets of human skeletal muscle samples collected from 20 insulin sensitive individuals before and after insulin treatment in order to construct insulin-mediated regulatory network. Based on a motif discovery method implemented by iRegulon, a Cytoscape app, we identified 25 candidate regulons, motifs of which were enriched among the promoters of 478 up-regulated genes and 82 down-regulated genes. We then looked for a hierarchical network of the candidate regulators, in such a way that the conditional combination of their expression changes may explain those of their target genes. Using Genomica, a software tool for regulatory network construction, we obtained a hierarchical network of eight regulons that were used to map insulin downstream signaling network. Taken together, the results illustrate the benefits of combining completely different methods such as motif-based regulatory factor discovery and expression level-based construction of regulatory network of their target genes in understanding insulin induced biological processes and signaling pathways.

Performance Characteristic of the β-Human Chorionic Gonadotropin Assay on the Access 2 for Its Use in Doping Control

강민정,Hyeim Lee,Sang Mi Shin,유혜현,권오승,진창배,정봉철 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.10

The objective of this study was to validate performance characteristics of the Access 2 (Beckman coulter) system for hCG assays for use as a confirmation test for doping control. The Access 2 assay was linear up to 500 IU/L. The correlation coefficient was higher than 0.999, and the sensitivity of the linearity was 0.82. There were no false positive or false negative cases. LOD was 0.59 IU/L. The method was robust when performed by different people. Repeatability and reproducibility were below 7%. We compared reproducibility and recoveries of Access 2 and Elecsys 2010. Access 2 demonstrated higher reproducibility than Elecsys 2010. Recoveries (accuracy) of Access 2were between 85 and 105%. Recoveries for Elecsys 2010 were between 91 and 104%.

Changes in The Content of Organic Acids in Enzyme-Treated Wheat Germ Extract

Jaekang Lee,Hyeim Kum,Dongwoo Kang,Younghyun Choi,Yongseok Choi 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

2배체 대장균의 제조와 그 특성

정혜임,임동빈,Jung, Hyeim,Lim, Dongbin 한국미생물학회 2016 미생물학회지 Vol.52 No.4

대장균에서 6개의 Leu 코돈중 가장 흔한 코돈은 CUG이다. 이 코돈을 인식하는 tRNA는 4개의 유전자에 의해 합성되는데, leuPQV와 leuT 2개의 locus로 나누어져 있다. 이 CUG를 인식하는 모든 tRNA가 결핍된 균주를 만들기 위해, 우선 leuPQV가 삭제된 균주(${\Delta}leuPQV$)와, leuT의 anticodon CAG를 GAG로 돌연변이시킨 균주[$Km^R$, $leuT^*$(GAG)]를 각각 만들었다. 이 두 돌연변이 유전자를 모으기 위해 ${\Delta}leuPQV$ 균주를 recipient로, $leuT^*$(GAG) 균주를 donor로하는 transduction을 수행한 결과, 콜로니 크기가 큰 것과 작은 것 두 종류의 transductant를 얻었다. PCR 후 염기서열 분석 결과 큰 콜로니는 예측한 recombinant로 판명됐으나, 작은 콜로니는 donor와 recipient 염색체 간의 상호교환재조합(reciprocal recombination)으로는 설명이 되지 않는, 돌연변이 유전자[$leuT^*$(GAG)]와 야생형 유전자(leuT(CAG)]를 모두 가진 균주로 밝혀졌다. 이 heterozygous diploid는 광학현미경으로 관찰시 세포의 형태와 크기에서 특이점이 발견되지 않았으나, 영양배지에서 야생형에 비해 생장이 한참 느리면서, 선형생장곡선(linear growth curve)이라는 예측하지 못한 생장특성을 보였다. 이 2배체 균주는 선택배지에서는 항상 작은 균일한 콜로니를 형성하였으나, 배지에 선택항생제 없을 경우, $leuT^*$(GAG) 유전자형 세포와 leuT(CAG) 유전자형 세포로 분리가 일어났다. 우리의 결과를 종합해볼 때, 이 2배체 균주는, $leuT^*$(GAG)와 leuT(CAG) 부분만 2배체로 갖는 부분이배체(merodiploid)라기 보다는, $leuT^*$(GAG)와 leuT(CAG)가 서로 다른 염색체에 있는 완전이배체라는 모델을 지지했다. 우리는 이러한 2배체가 어떻게 생성되었으며, 어떻게 분리되는지, 또 이 균주는 왜 선형생장곡선을 보이는지 등에 대한 모델을 토론하였다. Among 6 leu codons, CUG is the most frequently used codon in E. coli. It is recognized by leu-tRNA(CAG) encoded by four genes scattered on two chromosomal loci (leuT and leuPQV ). In the process of constructing a strain with no functional leu-tRNA (CAG) gene on chromosome, we made two mutant strains separately, one on leuPQV locus (${\Delta}leuPQV$), and the other on leuT locus [$leuT^*$(GAG)], where the anticodon of leuT was changed from CAG to GAG, thereby altering its recognition codon from CUG to CUC. We attempted to combine these two mutations by transduction using $leuT^*$(GAG) strain as a donor and ${\Delta}leuPQV$ strain as a recipient. Large and small colonies appeared from this transduction. From PCR and DNA sequencing, large colony was confirmed to be the reciprocal recombinant as expected, but the small colonies contained both mutant $leuT^*$(GAG) and wild type leuT (CAG) genes in the cell. This heterozygous diploid strain did not show any unusual morphology under microscopic observation, but, interestingly, it showed a linear growth curve in rich medium with much slower growth rate than wild type cell. It always formed homogenous small colonies in the selection medium, but, when there was no selection, it readily segregated into $leuT^*$(GAG) and leuT (CAG). From these observations, we suggested that the strain with both $leuT^*$(GAG) and leuT (CAG) genes was not a partial diploid (merodiploid), but a full diploid cell having two different chromosomes. We proposed a model explaining how such a heterozygous diploid cell was formed and how and why its growth showed a linear growth curve.

Effect of R-C Compensation on Switching Regulation of CMOS Low Dropout Regulator

Namsoo Kim,Ikguen Choi,Hyeim Jeong,Junho Yu 한국전기전자재료학회 2016 Transactions on Electrical and Electronic Material Vol.17 No.3

Miller feedback compensation is introduced in a low dropout regulator (LDO) in order to obtain a capacitor-freeregulator and improve the fast transient response. The conventional LDO has a limited bandwidth because of thelarge-size output capacitor and parasitic gate capacitance in the power MOSFET. In order to obtain a stable frequencyresponse without the output capacitor, LDO is designed with resistor-capacitor (R-C) compensation and this isachieved with a connection between the gain-stage and the power MOS. An R-C compensator is suggested to providea pole and zero to improve the stability. The proposed LDO is designed with the 0.35 μm CMOS process. Simulationtesting shows that the phase margin in the Bode plot indicates a stable response, which is over 100o. In the loadregulation, the transient time is within 55 μs when the load current changes from 0.1 to 1 mA.

Effect of R-C Compensation on Switching Regulation of CMOS Low Dropout Regulator

Choi, Ikguen,Jeong, Hyeim,Yu, Junho,Kim, Namsoo The Korean Institute of Electrical and Electronic 2016 Transactions on Electrical and Electronic Material Vol.17 No.3

Miller feedback compensation is introduced in a low dropout regulator (LDO) in order to obtain a capacitor-free regulator and improve the fast transient response. The conventional LDO has a limited bandwidth because of the large-size output capacitor and parasitic gate capacitance in the power MOSFET. In order to obtain a stable frequency response without the output capacitor, LDO is designed with resistor-capacitor (R-C) compensation and this is achieved with a connection between the gain-stage and the power MOS. An R-C compensator is suggested to provide a pole and zero to improve the stability. The proposed LDO is designed with the 0.35 μm CMOS process. Simulation testing shows that the phase margin in the Bode plot indicates a stable response, which is over 100^o. In the load regulation, the transient time is within 55 μs when the load current changes from 0.1 to 1 mA.

COLOURS OF INTERIOR MATERIALS AND STAFF FASHION IN RETAIL SPACE

Dongeun Hwang,Seunghan Jeong,Hyeim Jun,Unsol Choi,Jooeun Sung 글로벌지식마케팅경영학회 2019 Global Fashion Management Conference Vol.2019 No.07