오렌지로부터 아플라톡신B₁의 정량분석에 관한 연구

장향동,백광균,구경림,옥선미,최진석,고은주,류성렬 한국공업화학회 2004 응용화학 Vol.8 No.2

Aflatoxin B₁ was known to have the strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature, relative humidity and incubation days, orange incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20℃, 25℃, 30℃ at relative humidity 60%, 70%, 80% and for incubation time 3, 4, 5, 6, 7 days.. Production of Aflatoxin B₁ shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 77.41ppm, and by using Fluoroscence detector was 87.87ppm. - Production of Aflatoxin B₁ shows that the lowest production appreared at 20℃ and its relative humidity 80%(HPLC), 60%(Fluoroscence detector). The result that was quantitatively analyzed by using HPLC was 4.47ppm, and by using Fluoroscence detector was 4.17ppm. The structure of Aflatoxin B₁ was identify with authentic sample by using instrumental analysis methods of GC/MS, ¹H-NMR, FT-IR respectively.

A flatoxin B_1의 化學的 脫毒素化에 關한 硏究(第Ⅳ報)

張香東 단국대학교 대학원 1981 學術論叢 Vol.5 No.-

On acidification of aflatoxin B_1, the lactone ring in reformed and can be extracted with chloroform (2nd report). After hydrogen peroxide treatment of the aflatoxin B_1 in alkaline medium can not be extracted by chloroform. This process dependent upon the opening of the lactone ring in pure crystall aflatoxin B_1 are effectively distroyed on pH=9 and pH=10, 100℃, and 0.125M―H_2O_2 The lactone ring of aflatoxin B_1 opens in the hydrogen peroxide by alkaline medium to yield the carboxyl and hydroxyl group. The separation of opened compound was carried out by elution chromatography. The identification of opened compound was determined to molecular weight by titration and Rast method, and it was confirmed by infrared spectroscopy.

Dong-Chun Lecture : Cell-based High Content Screening (HCS) for action modes of drug targets by using systematic deletion mutants of Schizosaccharomyces pombe

( Dong Uk Kim ),( Im Seon Woo ),( Mi Sun Won ),( Young Joo Jang ),( Kyung Sook Chung ),( Han Oh Park ),( Hyang Sook Yoo ),( Kwang Lae Hoe ),( Doo Sik Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Aflatoxin B_1의 化學的 脫毒素化에 關한 硏究 (第Ⅱ報)

張香東 단국대학교 대학원 1980 學術論叢 Vol.4 No.-

Aflatoxin B_1 was transformed into aflatoxin B_2a by adjusting sulfuric acid solutions to pH=1, 2 and 3, and by heating over a of 30∼100℃. Aflatoxin B_2a was identified by co-thin layer chromatography using authentic B_2a and two different solvents. The transformation of aflatoxin B_1 was found to a function of pH, temperature and time. The optimum condition in this experiments were effected with the reaction under pH=1 and 100℃. The rate of disappearance of aflatoxin B_1 at given temperature and at Constant pH was found to be first order reaction.

수수로부터 아플라톡신B₁ 정량분석에 관한 연구

정향동,원옥남,구경림,류성렬 한국공업화학회 2003 응용화학 Vol.7 No.2

Aflatoxin B₁was known to have the strongest poisonous character among Aflatoxn compounds. To search the production of Aflatoxin B₁according to the change of temperature and relative humidity, millet incubated with Aspergillus flavus KCCM 35078 was incubated at 20℃, 25℃, 30℃, 25℃ and at relative humidity 50%, 60%, 70% and for incubation time 72, 96, 120, 144, 168, 192 hours. Production of Aflatoxin B₁shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 23.35ppm, and by using fluorescence detector was 23.50ppm Production of Aflatoxin B₁shows that the lowed production appeared at 20℃ and its relative humidity 50%. The result that was Quantitatively analyzed by using HPLC was 6,26ppm, and by using fluorescence detector was 6.33ppm The structure of Aflatoxin B₁was identify with authentic sample by using instrumental analysis methods of MS, H-NMR, FT-IR, respectively.

호박씨 기름으로부터 Sterol 성분들의 분석에 관한 연구

장향동,김천수,백광균,류성렬 한국공업화학회 2003 응용화학 Vol.7 No.2

It is found plant oils except Beta-sitosterol have almost the same Carbon contents, approximately 77.50%, comparing carbon contents of various oils by elemental analyzers, which means it is not possible to classify mixed oils using only elemental analysis. In addition, sulphur is not detected. It is proved that various sterol compositions exist in pumpkin seed oil in result of High Performance Liquid Chromatography(HPLC), also showing same sterol contents between Italian and Chinese pumpkin seed oils. However, it is not detectable whether other kinds of adulteration oils are mixed in pumpkin seed oil. Recently, pumpkin seed oil extract is also applied in therapy of disorders of the prostate gland and the urinary bladder. The therapeutic use and safety of a pumpkin seed oil extract were investigated in a clinical study with 2245 patients suffering from Benign Prostatic Hyperplasia. Evaluation of a the study was accomplished according to the International-Prostate-Symptom-Score(I-PSS), which decreased by 41.4%, while live quality improved by 46.1% during the treatment.

감초로부터 Glycyrrhizin 추출

이진휘,장향동,최하영,서동호,이성미,김경하 서울産業大學校 1998 논문집 Vol.47 No.1

Glycyrrhiza uralensis계의 원감초 뿌리에서 추출도는 감초는 여러가지 질병의 약제로 널리 이용되어 왔다. 그 주성분인 gliycyrrhizin은 항염증 활성 및 anti-allergic activity를 갖고 있으며 이러한 생리활성은 aglycone β-gliycyrrhetinic acid 의 활성에 의한 것으로 알려져 있고, β-gliycyrrhetinic acid는 steroid 구조와 유사하며, 면역조절 특성을 갖는 것으로 인식되고 있다. 이러한 β-gliycyrrhetinic acid 의 면역조절 활성에 기여하는 인자와 그 경로에 대하여 조사된 바 있다 Glycyrrhizin은 감초뿌리의 주성분중 하나이며, 그외에도 surgars(14%), starches(30%), flavonoids, saponoids, amonoacids, gums 및 essential oil 등을 포함하고 있다. Licorice, the root extract of Glycyrrhiza uralensis, is used as a medicine for various diseases. Antiinflammatory as well as anti-allergic activities have been attributed to one of its main constituents, glycyrrhizin. These activities are mainly ascribed to the action of the aglycome β-glycyrrhetinic acid. βGlyeyrrhetinic acid has a steroid-like structure and is believed to have immunomodulatory properties, To determine whether interference with complement functions may contribute to the immunomdulatory activity of β-glycyrrbetinic acid. its effect on the classical and alternative activation pathways of human complement were investigated. The main constituent found in the root is glycyrrhizin. The plant also contains various sugars(14%), starches(30%), flavonoids, saponoids, aminoacids, gums, and essential oil etc.

Aziridine 化合物의 合成에 關한 硏究

崔世千,張香東 단국대학교 대학원 1982 學術論叢 Vol.6 No.-

Five steps of subsequent reactions: olefin, β-haloalcohol, epoxide, amino alcohol, β-haloamine and aziridne were conducted successfully. Among these reactions, amino alcohol, which were obtained by reaction of epoxide compounds with tert-butylamine and ethylamine were able to be separated quantitatively from the aqueous solution by saturating it with potassium carbonate, magnesium sulfate and sodium sulfate. The aziridine ring formation was unexpectedly difficult presumably due to the ring strain, even through proximity factor is favored for the small ring. The yield was improved by removing sulfur dioxide completely, which was generated during the chlorination prior to ring forming reaction and by using conc·potassium hydroxide during the aziridine formation.

Polygoum Tinctoria와 합성 인디고 염료의 물리화학적 특성 비교

최창용,장미경,공병기,최혜영,나상권,이동병,양숙향,나재운 한국공업화학회 2002 응용화학 Vol.6 No.2

The natural dye substances appear to have a lot of benefits that enable the dyed materials to exhibit their natural colors. It may have a bit of a harmful influence on the human body itself, in comparison with the synthetic substances. It also causes hardly any environmental pollution because of the natural chemicals and costs much less to disposal. In this study was investigated physicochemical characterization of polygoum tinctoria dye substance and synthetic indigo. Calcium hydroxide was added into polygoum tinctoria leaves to precipitate dye substances and it was freezing dried into powder form. Characterizations of dye substances were investigated by UV/VIS spectrophotometer, TLC, DSC, TGA, HPLC, and MALDI-TOF Mass. Resultantly, polygoum tinctoria was confirmed that is consisted of two ingredients from these results.

Benzene에 의해 오염된 물의 鹽素處理에 의한 鹽素化合物의 生成에 關한 硏究

신현진,최고열,장향동 서울産業大學校 1996 논문집 Vol.44 No.1

This paper describes the production of the second pollution materials possibly produced during the water purification of water contaminated by various inorganic compounds. The effect of the reaction conditions such as the concentration of benzene and remaining chloride, temperature, pH as well as the addition of heavy metal on the production of chlorobenzenes has been investigated. It was confirmed from the results of quantitative and qualitative analyses of products that mono- and dichlorobenzenes, which smelled offensively and were harmful to human body, were created by chlorination of benzene containing water. The production of chlorobenzene was always observed when the concentration of benzene and remaining chloride was higher than 0.1 mg/ℓand 1 mg/ℓ, respectively. The amount of chlorobenzene increased with the reaction temperature and the concentration of reactants. The production of dichlorobenzene was largely dependent on the reaction temperature and the pH of reactant. When the pH of reactant was 2, an appreciable amount of 1.4-dichlorobenzene was produced. However, it decreased rapidly with the pH of reactant and completely disappeared at pH 4. There was no chlorobenzene produced at higher than pH 10. The concentration of chlorobenzene increased by the addition of Al and Fe.