複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

치료경험이 있는 A형 혈우병 환자에서 그린모노^�의 약동학 및 안전성 : 전향적 다기관 공동 임상시험

윤휘중,이순용,황태주,손영택 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

배 경 : 최근 국내에서 사용 가능해 진 단클론항체를 이용한 고순도의 제 8응고인자 그린모노^R에 대하여, 제품의 약동학적 측면을 관찰하고, 급성 이상반응 발생 측면의 안전성을 관찰하고자 하였다. 방 법 : 과거 응고인자 치료경험이 있는 제 8응고인자치 5% 미만의 A 형 혈우병 환자를 대상으로, 그린모노를 체중 1kg당 50units 정맥주사한 후 약물역동학적 분석을 시행하였다. 약물 투여 후 48시간까지 이상반응을 관찰하고, 약물투여 전 및 48시간 까지 이상반응을 관찰하고, 약물투여 전 및 48시간 후에 일반혈액 검사, 혈액생화학검사, 요검사 등을 측정하여 비교하였다. 제8응고인자 억제인자를 Bethesda assay를 이용하여 투여 전 및 투여 3~7일 후 검사하였다. 결 과 : 15명의 환자중 연구를 완료한 13명의 자료를 분석하였다. Recovery rate는 99±22%(범위, 71~ 136%) 였으며, 2-compartment model을 이용한 beta phase의 반감기는 15.7±6.6시간(범위, 9.7~35.9시간)이었다. 그린모노^ R 투여후 의미있는 이상반응은 없었으며, 검사성적의 의미있는 변화도 발견할 수 없었다. 제8응고인자 억제인자는 시험약 투여 전후 모두 0.6 BU 미만으로 유지되었다. 결 론: 그린모노^R는 약동학적으로 유효하고, 급성이상 반응이 없어, 임상 이용에유용하리라 생각한다.

Stem Cell Factor(SCF)와 Interleukin-3(IL-3)

윤휘중 대한내과학회 1999 대한내과학회지 Vol.57 No.4

최신 항암제

윤휘중 경희대학교 2002 慶熙醫學 Vol.18 No.2

Thrombopoietin이 제대혈의 CD34 양성 조혈모세포의 거핵구계 세포분화에 미치는 영향

서진태,조경삼,윤휘중,김시영,이우인 경희대학교 2000 慶熙醫學 Vol.16 No.2

이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향

황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.

심초음파로 결정된 응급 심낭천자술의 천자부위

김성환,황성오,이강현,조준휘,강구현,문중범,이승환,윤정한,최경훈,김영식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: The aim of this study was to determine whether the conventional subcostal approach is suitable for emergency pericardiocentesis in patients with cardiac tamponade or impending cardiac tamponade. Methods: This study was a prospective, observational study conducted at the emergency department of a tertiary hospital, Patients who had symptomatic pericardial effusion and who needed emergency pericardiocentesis in the emergency department were included in this study. We measured the epicardium-to-pericardium distance at the subcostal, parasternal, and apical area with two-dimensional echocardiography to determine the appropriate puncture site for pericardiocentesis. An epicardium-to-pericardium distance of more than 1.0 cm was considered as the primary safety factor in determining the Puncture site for pericardiocentesis. The skin-to-pericardium distance was considered as secondary safety factor. Results: Ninety-five consecutive patients(55 males and 40 females; total mean age: 53 year old) with cardiac tamponade or impending cardiac tamponade were enrolled in this study. The puncture site for pericardiocentesis, as determined by echocardiography, was the subcostal area in 43 patients(45%), the apical area in 40 patients(42%), the left parasternal area In 11 patients(12%), and the right parastemal area in one patient(1%). Pericardiocentesis failed in 2 patients(2%) with the subcostal approach and in one patient(1%) with the apical approach. The average epicardium-to-pericardium distance was 31 ±21 mm in patients with the subcostal approach and 21±8 mm in patients with other approaches. There were no differences in the amount of pericardial fluid and in the intraperical pressure among patient groups according to puncture site. There were two procedure related complications: a puncture of the right ventricle with the subcostal approach and a ventricular tachycardia with the apical approach.

심포지움 : Stem Cell Factor(SCF)와 Interleukin-3(IL-3)

윤휘중 ( Hwi Joong Yoon ) 대한내과학회 1999 대한내과학회 추계학술대회 Vol.57 No.-

중증 과립구 감소증을 동반한 환자에서 Fluconazole의 진균감염 예방 효과

윤휘중,김정백,어완규,김시영,조경삼 대한감염학회 1993 감염 Vol.25 No.1

Fungal infection is one of the major cause of mortality in neutropenic patients. We performed clinical studies to assess the effectiviveness and toxicity of fluconazole for prophylaxis of fungal infection in 20 neutropenic patients, who were admitted to Kyung Hee University Hospital from June 1991 to June 1992 and who had a high risk of fungal infection. The results were as follows. 1) In 18 case, there was no evidence of fungal infection during the hospital course. One patient had suspicious fungal infection on chest X-ray and clinical finding, and the other case had fungemia confirmed by blood culture. Two patients died of septicemia. 2) Elevation of AST, LAT (15%) G-I trouble (10%) were obserbed as toxicities of fluconazole, but renal dysfuntion, skin rash, or seizure was not obserbed.

The relationship between low survival and acute increase of tumor necrosis factor expression in the lung in a rat model of asphyxial cardiac arrest

Yoon soo Park,Hyun Jin Tae,Jeong Hwi Cho,In Shik Kim,Taek Geun Ohk,Chan Woo Park,Joong Bum Moon,Myoung Cheol Shin,Tae Kyeong Lee,Jae Chul Lee,Joon Ha Park,Ji Hyeon Ahn,Seok Hoon Kang,Moo Ho Won,Jun Hw 대한해부학회 2018 Anatomy & Cell Biology Vol.51 No.2

Cardiac arrest (CA) is sudden loss of heart function and abrupt stop in effective blood flow to the body. The patients who initially achieve return of spontaneous circulation (RoSC) after CA have low survival rate. It has been known that multiorgan dysfunctions after RoSC are associated with high morbidity and mortality. Most previous studies have focused on the heart and brain in RoSC after CA. Therefore, the aim of this research was to perform serological, physiological, and histopathology study in the lung and to determine whether or how pulmonary dysfunction is associated with low survival rate after CA. Experimental animals were divided into sham-operated group (n=14 at each point in time), which was not subjected to CA operation, and CA-operated group (n=14 at each point in time), which was subjected to CA. The rats in each group were sacrificed at 6 hours, 12 hours, 24 hours, and 2 days, respectively, after RoSC. Then, pathological changes of the lungs were analyzed by hematoxylin and eosin staining, Western blot and immunohistochemistry for tumor necrosis factor  (TNF-). The survival rate after CA was decreased with time past. We found that histopathological score and TNF- immunoreactivity were significantly increased in the lung after CA. These results indicate that inflammation triggered by ischemia-reperfusion damage after CA leads to pulmonary injury/dysfunctions and contributes to low survival rate. In addition, the finding of increase in TNF- via inflammation in the lung after CA would be able to utilize therapeutic or diagnostic measures in the future.