Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

Dysregulation of the enteric nervous system may play an important role in C3 deficiency-induced constipation in the mid colon of C3 KO mice

Hee Jin Song,Yun Ju Choi,Ji Eun Kim,You Jeong Jin,Yu Jeong Roh,Ayun Seol,Su Jin Lee,Dae Youn Hwang1,2 한국실험동물학회 2023 한국실험동물학회 학술발표대회 논문집 Vol.2023 No.2

도시 주부의 생활환경 문제에 대한 의식과 관리행동

황경혜,정진희,이정우 숙명여자대학교 생활과학연구소 1993 生活科學硏究誌 Vol.8 No.-

The purpose of this study is to investigate the urban housewives and the level of their consciousness and management in the problems of living environment, and to analize the major factors which make various effects. On the findings of these data, author myself is to inspire the environmental accountability and the role in environment preservation, and to provide the grounding materials, by which housewives are realistically awakened to their new significance in the environment problems in the life area. The period of investigation was a month in august, 1993, and the subject of it was limited to the housewives who were living in Kwang-Ju. the 650 sheets of questionnaires were distributed to them and the 536 sheets of them were used in research materials. The data which was obtained got the frequency percentage and the arithmetic mean by way of SAS, and were analyzed by F-test, Duncan's multiful rangement, Pearson's coefficient analysis and stepwise multiful regression. The major findings is summarized as follows: 1) The average score of housewives consciousness and management behavior on the problems of living environment are 74.14 on the scale of 115 (64.46 on the scale of 100) and 69.80 on the scale of 115 (60.69 on the scale of 100). 2) Housewives'age, education and income, the attitude on the household labor, the satisfaction in the family life and the perceptibility in the time pressure are the variables which have an influence on their consciousness in the problems of the living environment, while their age, education, the satisfaction of the family life and the attitude on the household labor are the variables which have an influence on their management behavior in them. 3) There are relatively a positive correlationship between their consciousness and management behavior in the problems of the living environment. 4) It appears that their management behavior on the problems in the living environment has a major correlationship with the consciousness on them, the education, the income and the attitude on the household labor. Based on the above result, the change of consciousness are required in the problems of the living environment. Accordingly, we should develop enough programs and materials in the environment education, and take the lifelong education and the data development.

皮日休 思想의 硏究

황정희 中國語文硏究會 1995 中國語文論叢 Vol.9 No.-

콩나물의 품종, 뿌리발생 및 재배일수에 따른 asparagine 함량 변이

정연신,박희준,Krishna Hari Dhakal,이정동,이인중,황영현 경북대학교 농업과학기술연구소 2008 慶北大農學誌 Vol.26 No.-

숙취해독에 탁월한 효과를 지니는 asparagine 함량의 콩나물 계통간 차이와 뿌리 발생량에 따른 차이 및 콩나물 재배기간에 따른 차이를 구명하여 고 asparagine을 가지는 기능성 콩나물 생산과 품종육성을 위하여 국내재래 콩나물 계통의 asparagine함량을 분석한 결과를 요약하면 다음과 같다. 1. 공시된 나물콩 174품종 및 계통의 콩나물에서의 sparagine 함량의 범위 및 평균은 0.38-1.67%, 0.99%(생체수준)로 각각 나타나 다양한 변이를 보였다. 2. 공시된 장려품종과 재래계통의 asparagine함량은 각각 1.29, 0.96% (생체수준)로 재래계통보다 장려 품종에서 높게 나타났다. 3. 백립중별 asparagine함량은 8.1~10.0g범위에서 1.02%(생체수준)로 가장 높은 경향이었으나 유의적인 차이는 인정되지 않았다. 4. 종피색별 asparagine 함량은 갈색종피종이 1.15%(생제수준)로 가장 높았으며 다른 종피종들은 비슷한 경향으로 나타났다. 5. 공시된 장려품종의 asparagine 함량의 범위는 4.08~6.24%(건물수준)로 나타났고, 다원콩(6.24%), 소백나물콩(6.21%), 소원콩(5.95%), 소명콩(5.85%) 순이 었다. 6. 콩나물의 재배일수를 10일로 한 경우 asparagine함량과 관계가 깊은 뿌리발생량이 크게 증가하였다. 7. 재배일수에 따른 asparagine함량은 5일재배와 10일재배에서 각각 0,68%, 1.21%(생제수준)로 나타나 재배 일수간 asparagine 함량에는 고도의 유의적인 차이가 인정되었다. 8. 콩나물의 하배측 부위에서 재배일수가 증가할수록 asparagine 함량도 증가하여 2일 재배시 2.91%(건물수준)이던 것이 14일 재배시에는 15.68%까지 증가하였다. The varietel difference, effect of root amounts and cultivation duration on the asparagine content of soybean sprouts, which is known to have the excellent detoxifying effect of ethanol, were investigated for developed varieties and indigenous sprout lines to establish cultivation methods of increasing the asparagine content and to develop soybean varieties having high asparagine content. Some of the research results obtained are summarized as follows; 1. Range and mean of asparagine content of 174 germplasm were 0.38~1.67% and 0.99%, respectively, on fresh weight basis. 2. Developed sprout-soybean varieties showed somewhat higher asparagine content of 1.29% than that of indigenous sprout lines of 0.96% on fresh weight basis. 3. No significant difference in asparagine content among the seed size groups was recognized though the highest asparagine content, 1.02% on fresh weight basis was observed in the seed size of 8.1~10.0g/100 seeds. 4. Among the seed coat color groups, soybean of brown seed coat color showed the highest asparagine content (1.15%) on fresh weight basis. No difference was observed among other groups of seed coat color. 5. Range of asparagine content of 174 varieties was 4.08~6.24% on dry weight basis. Soybean varieties that showed high asparagine content were Dawonkong, Sobacknamulkong, Sowonkong, and Somyungkong, with the contents of 6.24%, 6.21%, 5.95%, and 5.85%, respectively. 6. Amount of roots which have the highest asparagine content out of sprout parts was greatly increased up to 10 days of sprout cultivation. 7. Highly significant difference in asparagne content of sprout was recognized between those grown for five and ten days, with the asparagine content of 0.68% and 1.21%, respectively, on fresh weight basis. 8. Asparagine content of hypocotyle part was also highly proportional to days to cultivation; it increased from 2.91% at 2 days of cultivation to 15.68% on fresh weight basis at 14 days of cultivation.

바닐로이드 수용체 길항제로서의 (1R)-phenethyl thiocarbamic acid 0-[l-(4-hydroxy-3-methoxyphenoxymethyI)-3-phenylpropyl] ester 의 설계 및 합성

황유정,이현주,김희두 숙명여자대학교 약학연구소 2002 약학논문집-숙명여자대학교 Vol.18 No.-

(IR)- Phenethylthiocarbamic acid 0-[l-(4-hydroxy-3-methoxyphenoxymethyl)-3-phenylpropyl] ester (8) was designed and synthesized as a vanilloid receptor antagonist. Acetovanillone (1) was successively converted to the thiocarbamate 8 via 6-steps sequence including Baeyer-Villiger oxidation and copper(I)-catalyzed epoxide ring opening reaction.

금피복된 임플란트 보철용 유지나사의 예비하중 및 풀림회전력에 관한 연구

정창모,황희성,전영찬,최설희 大韓齒科器材學會 2000 대한치과재료학회지 Vol.27 No.3

The purpose of this study was to compare the preload and the removal torque produced when using gold coated prosthetic retaining screws (Gold-Tite retaining screws) and non-coated screws(gold screws) repeatedly and to evaluate effects of a thin pure gold coating on screw stability. Following connection of the conical abutment to implant fixture using 20 Ncm of torque applied by a torque gauge, fastened the strain-gauged gold cylinder to conical abutment with gold screw, Gold-Tite retaining screw using 10Ncm. Then, preloads and removal torques were measured, screws tightened and loosened 8 times repeatedly. All experiments were performed under dry and wet condition. The results obtained were summarized as follows : 1. For gold screw group, no significant differences existed in the preload and the removal torque according to trials in both conditions(p>0.05). 2. For Gold-Tite retaining screw group, no significant differences existed in the preload according to trials in both conditions(p>0.05). However, the removal torque decreased at the second trial and incresed, after then(p<0.05). 3. In comparison of preloads according to trials, the preload in dry condition was greater than in wet condition only for Gold-Tite retaining screw group after the fourth trial(p<0.05). However, no significant differences were found between gold screw group and Gold-Tite retaining screw group(p>0.05). 4. In comparison of removal torque according to trials, the removal torque of gold screw group was greater than of Gold-Tite retaining screw group in dry condition at the second, third trial(p<0.05). At the other trials, no significant differences were found among the experimental groups(p>0.05).

皮日休의 文學觀

황정희 中國語文硏究會 1995 中國語文論叢 Vol.8 No.-

통합의료정보시스템을 위한 접근제어모델

황정희,차효성 남서울대학교 2014 남서울대학교 논문집 Vol.20 No.1-2

Ensuring the security of medical record is becoming an increasingly important problem as modern technology is integrated into existing medical services. Electronic medical record system is established in independent private network, which provide certifications for medical practice. An outflow of confidential medical information is an invasion of privacy of the person. Advanced medical information system is integrated with unit of the system because user needs is various. It is necessary to control access management of health information system according to user role. In this paper, we propose an efficient and appropriate management authority system and also introduce the instances applied into a large hospital EMR system, developing appropriate access management. to match the characteristics of the integrated medical information system.

The effects of input-enhanced and output-enhanced grammar instruction on the learning of English verb tenses and relative clauses

Hwang,Hee Jeong 이화여자대학교 교과교육연구소 2014 교과교육학연구 Vol.18 No.3

The present study investigated whether different types of form-focused instruction, input-enhanced or output-enhanced, might be effective in improving low-proficiency learners' English knowledge about specific linguistic forms. The participants were 111 first-year Korean university students enrolled in an English conversation course. They were assigned to three groups; 35 students in the input-enhanced group (TIE), 33 in the output-enhanced group (DICT), and 45 in the control group (CG). English verb tenses and relative clauses were selected as the target structures. Pre- and post-tests were conducted to measure the effectiveness of different types of form-focused instruction on the acquisition of the target forms. Pre- and post-questionnaires were administered to examine the extent of the students' perceptions and attitudes towards the English instruction that they received. The study discovered that both input-enhanced and output-enhanced instruction were more effective than traditional grammar instruction in developing grammatical knowledge about verb tenses, while output-enhanced instruction was more effective than input-enhanced and traditional grammar instruction with respect to the learning of relative clauses. The results of the questionnaires indicated that learners who performed the TIE task and the dictogloss task showed positive changes in their perceptions about the English instruction that they received; only those in the dictogloss task showed positive changes in their attitudes towards the instruction. Based on the results, the limitations of the study and the pedagogical implications are provided.