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Hwang Sang Mee,Kim Beom Joon,Lee Jee-Soo,Seong Moon-Woo,Seo Soo Hyun,Paik Jin Ho,Kim Sang-A,Lee Ji Yun,Lee Jeong-Ok,Chang Yoon Hwan,Bang Soo Mee 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.6
Systemic mastocytosis with associated hematological neoplasm (SM-AHN) poses diagnostic challenges because of the coexistence of atypical mast cell proliferation and hematological neoplasms. We assessed the presence of SM-AHN in patients with acute myeloid leukemia (AML) with RUNX1::RUNX1T1 from 2014 to 2020. Bone marrow (BM) samples were evaluated for mast cell aggregates using CD117 and CD25 immunohistochemical (IHC) staining. The KIT D816V variant burden at diagnosis and post induction was assessed using droplet digital PCR. Among 23 patients diagnosed as having AML with RUNX1::RUNX1T1, four (17.4%) were also diagnosed as having SM-AHN. No significant differences in clinical characteristics or overall survival (P=0.565) were observed between patients with or without SM-AHN, except for the presence of KIT variants (P=0.040). After induction therapy, IHC staining revealed the presence of mast cell aggregates in the BM, and the KIT D816V variant burden decreased with decreasing blast count and was similar in BM aspirates, smear slides, and sections. Concomitant SM-AHN was not infrequent in AML patients with RUNX1::RUNX1T1. This study showed the importance of CD117 and CD25 IHC staining after induction chemotherapy for SM-AHN screening, especially in patients with KIT variants.
Hwang, Jee Youn,Kwon, Mun-Gyeong,Kim, Yu Jin,Jung, Sung-Hee,Park, Myoung-Ae,Son, Maeng-Hyun Elsevier 2017 Fish & shellfish immunology Vol.60 No.-
<P><B>Abstract</B></P> <P>Vaccination by immersion is suitable for mass vaccination of small size fish. However, no viral vaccine has been developed for immersion applications, because of low efficacy. In this study, we evaluated the efficacy and safety of immersion vaccine against viral hemorrhagic septicemia (VHS) containing Montanide IMS 1312 VG adjuvant in olive flounder (<I>Paralichthys olivaceus</I>). Healthy fish were vaccinated by an immersion method with a heat-inactivated FP-VHS2010-1 strain of VHS virus (VHSV) in combination with Montanide IMS 1312 VG for 5 min at 20 ± 2 °C. The control group was vaccinated with sterile PBS. No toxicity of immersion vaccine with Montanide IMS 1312 VG adjuvant was observed by hematological and histopathological analysis. Immersion vaccine with adjuvant enhanced gene expression of immune-associated genes, i.e., genes encoding interleukin (IL)-1β, IL-6, IL-8, and Toll-like receptor (TLR) 3. Relative percent survival (RPS) of fish was measured on weeks 4 and 8 post vaccination. In fish vaccinated with adjuvant, RPS was significantly higher than that of fish vaccinated without adjuvant. The results of the present study provide evidence that the VHSV immersion vaccine with Montanide IMS 1312 VG induces protective immunity in olive flounder against VHS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immersion vaccination of heat-inactivated VHSV with Montanide 1312 VG protected olive flounder from VHS. </LI> <LI> Immersion vaccination inceased the levels of several immune genes. </LI> <LI> Addition of Montanide 1312 VG further increased the levels of immune genes. </LI> <LI> Duration of protective effect of immersion vaccination was short. </LI> </UL> </P>
( Sang Ah Shin ),( Eun Young Park ),( Dong Han Sun ),( Tae Kyoung You ),( Myung Joo Lee ),( Soo Chan Hwang ),( Hee Young Paik ),( Hyo Jee Joung ) 한국임상영양학회 2014 Clinical Nutrition Research Vol.3 No.2
The accuracy of dietary assessments has emerged as a major concern in nutritional epidemiology and new dietary assessment tools using computer technology to increase accuracy have been developed in many countries. The purpose of this study was to develop a web-based computer-assisted personal interview system (CAPIS) for conducting dietary assessment and to evaluateits practical utilization among Koreans. The client software was developed using Microsoft``s ClickOnce technology, which allows communication with a database system via an http server to add or retrieve data. The system consists of a tracking system for the subject and researcher, a data-input system during the interview, a calculation system for estimating food and nutrient intake, a data-output system for presenting the results, and an evaluation system for assessing the adequacy of nutrient and food intake. Databases of the nutrient composition of common food (n = 3,642), recipes for common dishes (n = 1,886), and photos of serving sizes for food and dishes (n = 4,152) were constructed, and logical processes for data collection, calculation, and output were developed. The functionality, on-site applicability, and efficiency of CAPIS were evaluated in a convenience sample of 181 participants (61 males, 120 females; aged 24 to 85) by comparing with manual 24 hour recall method with paper questionnaire. The CAPIS was functioned adequately in the field survey in terms of completeness of function, security, and compliance of researcher and subjects. Regarding on-site applicability, 23.2%, 32.6%, 35.4%, and 43.7% of subjects reported that CAPIS was easier to recall their diet, to estimate the amount consumed, to communicate with the interviewer, and to concentrate on the interview than the manual method with paper questionnaire, respectively. Although CAPIS required more interview time (9 min 42 sec) compared to the manual method (7 min 30 sec), it saved time and cost for data coding and entry (15 min 35 sec) and gave high satisfaction from the prompt feedback after interview to the subjects, which increase ef- ficiency to apply on the field survey. Our results suggest that the newly developed CAPIS is suitable for conducting personal interviews for dietary assessment in Korean population.
Skin wound healing effects of (+)-syringaresinol from ginseng berry
Jee-hyun Hwang,Yeonsoo Kang,Heui-Jin Park,Seolyeong Kim,Su-Hyun Lee,Hangun Kim,Sang-Jip Nam,Kyung-Min Lim 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.5
Background: Ginseng has been used as a traditional medicine and functional cosmetic ingredients formany years. Recent studies have focused on the potential biological effects of the ginseng berry and itsingredients. (þ)-Syringaresinol (SYR) is enriched in ginseng berry and its beneficial effects on the skinhave been recently reported. However, little is known about the its effects on the wound healing processof skin. Methods: Here, we evaluated the skin wound healing effect of (þ)-SYR using the human fibroblast Hs68cell and ex vivo pig and human skin tissue model. Scratch wound test and hydrogen peroxide (HPO)induce chemical wound model were employed. Results: (þ)-SYR promoted the migration and proliferation of Hs68 cells without significant cytotoxicityat the tested concentrations. Especially, in ex vivo pig and human skin tissue, HPO-induced chemicalwound was recovered almost completely by (þ)-SYR. In line with the finding in Hs68, the proteinexpression levels of TGF-b and PCNA, a proliferation marker were increased, demonstrating the beneficialeffects of (þ)-SYR on skin wound repair. Conclusion: Collectively, we demonstrated that (þ)-SYR from ginseng berry, can enhance the woundhealing effect by accelerating cell proliferation and skin regeneration, suggesting the potential utility of(þ)-SYR for skin wound repair.
( Jee Won Hwang ),( Hyung Joon Yim ),( Hyun Jung Lee ),( Sun Jae Lee ),( Eileen L. Yoon ),( Sang Jun Suh ),( Ji Hoon Kim ),( Jong Eun Yeon ),( Soon Ho Um ),( Kwan Soo Byun ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Hepatic stellate cells (HSCs) are a major fibrogenic cell type that contributes to collagen accumulation during chronic liver disease. The NS-398 (a selective COX-2 inhibitor) is a potential drug of chemoprevention and treatment of liver fibrosis by inhibiting HSC activation. The simvastatin (a HMG CoA reductase inhibitor) revert the myofibroblasts-like phenotype of HSC towards a more quiescent phenotype. We investigated the anti-fibrotic and pro-apoptotic mechanisms in synergistic effects of both drugs as using the human hepatic stellate cell line. Methods: The immortalized human hepatic stellate cell line, LX-2 was cultured. The cells were treated with control, simvastatin, NS-398, and combination of simvastatin and NS-398 for 24 and 48 hours. Cellular DNA synthesis was evaluated by measuring BrdU incorporation. Expression of proteins including cell cycle regulators such as p21, cyclinD1 and Akt, NF-kB, caspase-3, caspase-9 as apoptotic factors, α -smooth muscle actin (α-SMA) were measured in Western blot analysis. Results: In BrdU-proliferation assay, combination of two drugs effectively inhibited the proliferation rate compared to monotherapy. The combination of two drugs resulted in a greater decrease of procaspase-3, -9, Akt and NF-kB than monotherapy depend on the concentration of two drugs. In addition, the combination of NS-398 and simvastatin significantly promote the inactivation of HSCs by reducing the amounts of α-SMA protein. Conclusions: We suggested that both of two drugs have synergistic effects that anti-proliferative, pro-apoptotic and anti-fibrotic effects on activated HSCs. The mechanism of synergistic pro-apoptotic effect seems to be mediated through inhibition of NF-kB/Akt pathway and caspase activity.
( Jee Won Hwang ),( Hyung Joon Yim ),( Hyun Jung Lee ),( Sun Jae Lee ),( Eileen L Yoon ),( Sang Jun Suh ),( Ji Hoon Kim ),( Jong Eun Yeon ),( Soon Ho Um ),( Kwan Soo Byun ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Department of Internal Medicine, Division of Gastroenterology and Hepatology, Korea University College of Medicine Background: Hepatic stellate cells (HSCs) are a major fibrogenic cell type that contributes to collagen accumulation during chronic liver disease. The NS-398 (a selective COX-2 inhibitor) is a potential drug of chemoprevention and treatment of liver fibrosis by inhibiting HSC activation. The simvastatin (a HMG CoA reductase inhibitor) revert the myofibroblasts-like phenotype of HSC towards a more quiescent phenotype. We investigated the anti-fibrotic and pro-apoptotic mechanisms in synergistic effects of both drugs as using the human hepatic stellate cell line. Methods: The immortalized human hepatic stellate cell line, LX-2 was cultured. The cells were treated with control, simvastatin, NS-398, and combination of simvastatin and NS-398 for 24 and 48 hours. Cellular DNA synthesis was evaluated by measuring BrdU incorporation. Expression of proteins including cell cycle regulators such as p21, cyclinD1 and Akt, NF-kB, caspase-3, caspase-9 as apoptotic factors, α -smooth muscle actin (α-SMA) were measured in Western blot analysis. Results: In BrdU-proliferation assay, combination of two drugs effectively inhibited the proliferation rate compared to monotherapy. The combination of two drugs resulted in a greater decrease of procaspase-3, -9, Akt and NF-kB than monotherapy depend on the concentration of two drugs. In addition, the combination of NS-398 and simvastatin significantly promote the inactivation of HSCs by reducing the amounts of α-SMA protein. Conclusions: We suggested that both of two drugs have synergistic effects that anti-proliferative, pro-apoptotic and anti-fibrotic effects on activated HSCs. The mechanism of synergistic pro-apoptotic effect seems to be mediated through inhibition of NF-kB/Akt pathway and caspase activity.
Chaperone-E3 Ligase Complex HSP70-CHIP Mediates Ubiquitination of Ribosomal Protein S3
Hwang, Inwoo,Cho, Sung-Woo,Ahn, Jee-Yin MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.9
<P>In addition to its role in ribosome biogenesis, ribosomal protein S3 (RPS3), a component of the 40S ribosomal subunit, has been suggested to possess several extraribosomal functions, including an apoptotic function. In this study, we demonstrated that in the mouse brain, the protein levels of RPS3 were altered by the degree of nutritional starvation and correlated with neuronal apoptosis. After endurable short-term starvation, the apoptotic function of RPS3 was suppressed by Akt activation and Akt-mediated T70 phosphorylation, whereas after prolonged starvation, the protein levels of RPS3 notably increased, and abundant neuronal death occurred. These events coincided with ubiquitination and subsequent degradation of RPS3, controlled by HSP70 and the cochaperone E3 ligase: carboxy terminus of heat shock protein 70-interacting protein (CHIP). Thus, our study points to an extraribosomal role of RPS3 in balancing neuronal survival or death depending on the degree of starvation through CHIP-mediated polyubiquitination and degradation.</P>