An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam

Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10

Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.

Exposure to Tobacco Advertising and Promotion among School Children Aged 13-15 in Vietnam - an Overview from GYTS 2014

Tran, Khanh Long,Phung, Xuan Son,Kim, Bao Giang,Phan, Thi Hai,Doan, Thi Thu Huyen,Luong, Ngoc Khue,Pham, Thi Quynh Nga,Nguyen, Tuan Lam,Hoang, Van Minh,Le, Thi Thanh Huong Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Evidence shows that tobacco advertising and promotion activities may increase tobacco consumption and usage, especially in youth. Despite the regulation on prohibiting advertisement of any tobacco product, tobacco advertisement and promotion activities are still common in Vietnam. This article presents current exposure to tobacco advertising and promotion (TAP) among school children aged 13 to 15 years in Vietnam in 2014 and potential influencing factors. Data from the Global Youth Tobacco Survey 2014 in Vietnam covering 3,430 school aged children were used. Both descriptive and analytical statistics were carried out with Stata 13 statistical software. Binary logistic regression was applied to explain the exposure to TAP among youth and examine relationships with individual factors. A significance level of p<0.05 and sampling weights were used in all of the computations. In the past 30 days, 48.6% of the students experienced exposure to at least 1 type of tobacco advertising or promotion. Wearing or otherwise using products related to tobacco was the most exposure TAP type reported by students (22.3%). The internet (22.1), points of sales (19.2) and social events (11.5) were three places that students aged 13-15 frequently were exposed to TAP. Binary logistic results showed that gender (female vs male) (OR = 0.61, 95%CI: 0.52 - 0.71), susceptibility to smoking (OR = 2.12, 95%CI: 1.53 - 2.92), closest friends' smoked (OR = 1.43, 95%CI: 1.2 - 1.7) and parents smoking status (OR = 2.83, 95%CI: 1.6 - 5.01) were significantly associated with TAP exposure among school-aged children. The research findings should contribute to effective implementation of measures for preventing and controlling tobacco use among students aged 13-15 in Viet Nam.

Preliminary Imaging Analysis for Enhanced Intestinal Uptake of Non-soluble Polystyrene Microspheres in the Presence of Oleic Acid using Rat Intestine

Huyen Thi Thanh Tran,Phuong Ha Lien Tran,Thao Truong Dinh Tran,Kyung Ho Lee,Beom Jin Lee 韓國藥劑學會 2009 Journal of Pharmaceutical Investigation Vol.39 No.3

The responsibility of C-terminal domain in the thermolabile haemolysin activity of Vibrio parahaemolyticus and inhibition treatments by Phellinus sp. extracts

Tran Thi Huyen,Ha Phuong Trang,Nguyen Thi-Ngan,Bui Dinh-Thanh,Le Pham Tan Quoc,Trinh Ngoc Nam 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.3

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 μg of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10–1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

Preliminary Imaging Analysis for Enhanced Intestinal Uptake of Non-soluble Polystyrene Microspheres in the Presence of Oleic Acid using Rat Intestine

Tran, Huyen Thi Thanh,Tran, Phuong Ha Lien,Tran, Thao Truong-Dinh,Lee, Kyung-Ho,Lee, Beom-Jin The Korean Society of Pharmaceutical Sciences and 2009 Journal of Pharmaceutical Investigation Vol.39 No.3

In vitro intestinal uptake of non-soluble polystyrene microspheres (NPMS) was visualized with and without oleic acid using a fluorescence microscopy. Fluorescent polystyrene latex microspheres with 1${\mu}$m larger size were used as models for nonspecifically absorbed nonbiodegradable particulates. The NPMS could not penetrate the enterocytes but a few NPMS could be penetrated via Peyer's patches. When the oleic acid was mixed with NPMS, the transporting efficiency of NPMS through enterocytes as well as Peyer's patches was significantly enhanced. The modification of the intestinal membrane permeability and surface feature of the NPMS in the presence of oleic acid might be a clue to the transport of NSPM although the detailed mechanism is still under investigation.

Violation of Bans on Tobacco Advertising and Promotion at Points of Sale in Viet Nam: Trend from 2009 - 2015

Le, Thi Thanh Huong,Tran, Khanh Long,Phung, Xuan Son,Do, Phuc Huyen,Phan, Thuy Linh,Nguyen, Ngoc Bich,Nguyen, Xuan Lam,Le, Vu Anh,Tran, Thi Tuyet-Hanh Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Comprehensive bans on tobacco advertising and promotion were introduced through tobacco control legislation in Viet Nam, but it has been established that violations of the bans are very common. This study was conducted to explore the trend in violations of bans on tobacco advertising and promotion at points of sale in Viet Nam in the past six years and to explore any differences in the violation situations before and after the Law on Tobacco Control came into effect on 1st May 2013. Quantitative data were collected through observation of violations of the bans on tobacco advertising and promotion at points of sale in 10 provinces throughout Viet Nam in four survey rounds (2009, 2010, 2011, and 2015). Variation in violation prevalence over time was examined by chi-square test using a Bonferini method. Binary logistic regression was employed to identify the factors that may have influences on different types of violation. A level of significance of p<0.05 was used for all tests in this article. The most common form of violation was the display of more than one pack/one carton of a cigarette brand. Violation of bans on tobacco advertising increased while violations on promotion ban and on displaying tobacco decreased through time. Some factors associated with the tobacco advertising and promotion bans included surveyed years, types of points of sale, regions and areas where the points of sale were located. The enforcement of the bans did not improve even after the issuance and the enactment of the Law on Tobacco Control. This suggests that the monitoring and enforcement of bans on tobacco advertising and promotion at points of sale should be strengthened. Penalties should be strictly applied for violators as indicated in the current tobacco control legislation.

Double burden of malnutrition in ethnic minority school-aged children living in mountainous areas of Vietnam and its association with nutritional behavior

Truong Duong Thuy Thi,Tran Trang Huyen Thi,Nguyen Tam Thanh Thi,Tran Van Hong Thi 한국영양학회 2022 Nutrition Research and Practice Vol.16 No.5

BACKGROUND/OBJECTIVES Although children of ethnic minority groups are experiencing a transition from a predominance of undernutrition to overnutrition, there is little evidence of a dual-malnutrition burden. Therefore, this study examined the double burden of malnutrition among school-aged ethnic minority children living in mountainous areas and its association with their diets. SUBJECTS/METHODS A cross-sectional study was conducted from September 2019 to March 2020 in 3 mountainous areas of Northern Vietnam among 1,556 ethnic minority school-aged children. The prevalence of under-nutrition (stunting and thinness) and over-nutrition (overweight and obesity) were measured using the WHO 2006 child growth standards (height-for-age and BMI-for-age Z-score). Nutritional practices were evaluated by the frequency of food consumption based on a 4-level scale. RESULTS The percentage of children with stunting and thinness were 14.0% and 5.4%, respectively, while the figure for overweight/obesity was 9.4%. The factors positively associated with stunting were living in a family with more than 2 children or being Muong/other ethnicities compared to the Tay ethnicity. Children who consumed fish/shrimps/crabs or milk weekly/daily were less likely to be undernourished compared to those who never consumed these foods. By contrast, children who never consumed foods rich in vitamin A precursors and vitamin A and fruit or consumed daily snacks/junk food were more likely to be overweight/obese. CONCLUSIONS Undernutrition remains a common issue among school-aged children and adolescents of ethnic minority groups, while over-nutrition exists simultaneously. Public health nutrition programs promoting adequate diets and positive lifestyle changes related to nutrition are essential to tackle the double burden of malnutrition among ethnic minority children.

Contrast Agents For Magnetic Resonance Imaging Based On Ferrite Nanoparticles Synthesized by Using a Wet-Chemical Method

Tran Hoang Hai,Le Hong Phuc,Doan Thi Kim Dung,Nguyen Thi Le Huyen,Bui Duc Long,Le Khanh Vinh,Nguyen Thi Thanh Kieu,Massanori Abe 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2

Magnetic resonance imaging (MRI) has become a major and promising topic in medical researching because of its numerous potential applications. Due to the specic uptake by macrophage and not entirely captured by liver and spleen at first-pass, Superparamagnetic nanoparticles (SPIONs) are widely investigated as diagnostic tracer for magnetic resonance imaging (MRI). Surfactantcoated Fe3O4 particles (6 nm diameter) have been synthesized by using a wet chemical method (co-precipitation). Our study concentrated on synthesizing magnetic nanoparticles Fe3O4 coating oleic acid and Dextran and Starch polysaccharides.

Factors Affecting Students’ Decision to Choose Regional Public Universities: An Empirical Study from Vietnam

Thi Thanh Thuy LE,Minh Tuan TRAN,Hoang Ba Huyen LE 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.4

The purpose of this paper is to investigate the impact of several factors on students’ decisions to attend a public institution in Vietnam’s North Central area. The enrollment issue toward regional institutions is particularly critical in the Vietnam Ministry of Education and Training reforming the university enrollment process and the complicated scenario of the Covid-19 pandemic. A total of 500 students were surveyed for research samples. Data is synthesized, validated, cleaned, and analyzed using SPSS and AMOS software using methods including reliability, EFA, CFA, and SEM. The findings suggest that the proposed independent components (individual factors, study fees, advertisement, infrastructure and facilities, local features, and lastly, training activities) have a beneficial impact on students’ decision to attend a public university in the North Central region. The study also found that the graduation exam outcome had a moderating effect on the relationship between registration and students’ decisions. These imply targeted solutions for regional public universities to diversify training majors, improve training quality, capitalize on local advantages, increase interaction, and promote training programs and image to be more effective in attracting students and maintaining competition in the current enrollment environment.

Effects of Supplemental Green LEDs to Red and Blue Light on the Growth, Yield and Quality of Hydroponic Cultivated Spinach (Spinacia oleracea L.) in Plant Factory

Nguyen Thi Phuong Dung(넨티덩),Tran Thi Thanh Huyen(트란티),Dong Cheol Jang(장동철),Il Seop Kim(김일섭),Nguyen Quang Thach(넨퀸탓) (사)한국생물환경조절학회 2020 생물환경조절학회지 Vol.29 No.2

본 연구는 폐쇄형 식물공장에서 시금치 수경재배시 세가지의 인공광이 생육, 광합성 및 품질에 미치는 영향을 규명하기 위해 수행되었다. 세가지 광 처리구는 적색 (660nm), 청색 (450nm) 및 녹색 (550nm) LED를 사용하여, R660 / B450 = 4/1 (RBL), R660 / B450 / G550 = 5/2/3 (WWL); R660 / B450 / G550 = 1/1/1 (WL) 비율로 혼합하였고, 동일한 광도로 설정하였다 (PPFD = 190 μmol‧m<SUP>-2</SUP>‧s<SUP>-1</SUP>). 생육조사결과 초장, 엽수는 WL이 가장 적었다. SPAD, 순광합성율, Fv/Fm, LAI, 근권부 생육은 RBL이 가장 높았고 통계적으로 유의하였다. 줄기, 잎, 뿌리의 생체중, 뿌리의 건물 중은 세가지 처리구에서 유의한 차이를 보였다. 대조적으로 WL의 칼륨의 함량은 WWL과 RBL 가운데 가장 높았지만, 반면 칼륨과 철의 함량은 RBL이 가장 높았다. 비타민C 함량도 시험구간 유의한 차이를 보였다. 질소와 옥살산 함량은 WL이 가장 높았고, 용해성 고체와 비타민C 함량은 RBL이 가장 높았다. 옥살산, 질소 함량은 WWL에서 감소하는 경향을 보였고, RBL의 옥살산 함량은 WL와 WWL과 차이가 없었다. 모든 처리구에서 Salmonella, E.coli. 는 감염되지 않았다. 결론적으로, RBL이 시금치의 생육에 적합하지만, 적색, 청색과 적정하게 혼합된 녹색광은 시금치의 생육에 긍정적인 영향을 미친다고 판단된다. The effect of three different light qualities on growth, photosynthesis, quality and safe parameters of hydroponic cultivated spinach (Spinacia oleracea L.) were investigated indoor. Three different light qualities were created of red (660 nm), blue (450 nm) and green (550 nm) LEDs corresponding at ratio R660/B450 = 4/1 (RBL); R660/B450/G550= 5/2/3 (WWL); R660/B450/G550 = 1/1/1 (WL), which were tested at the same intensity (PPFD =190 μmol m-2 s-1). The results showed that the plant height and leaf number were the lowest in WL treatment. The SPAD, Net photosynthesis rate Pn, Fv/Fm, Leaf area index LAI values and all parameters of root characteristics were the highest in RBL treatment and were significantly different from two others. Fresh weight of stem, leaf and root, dry weight of root in the three light qualities were significantly different. In contrast, the highest K<SUP>+</SUP> content in WL was different from WWL and RBL treatments, while Ca<SUP>2+</SUP> and Fe<SUP>2+</SUP> content were the highest in the RBL treatment. Vitamin C content was significantly different between the three treatments. nitrate and oxalic acid contents were the highest in WL treatment, whereas soluble–solids contents and vitamin C contents were the highest in RBL treatment. Oxalic acid, nitrate contents were observed tending reduced under WWL although oxalic acid content in RBL treatment was not different from WL and WWL treatments. In all three different light treatments were not detected Salmonella, E.coli. Our results suggest that RBL may be appropriate light for growth of spinach, but supplementary green light to a combination of red and blue LEDs at the reasonable rate can change the quality of spinach in a positive direction. Hydroponic cultivated spinach was safe for users.