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Thiocholine ester 기질을 이용한 Acetylcholinesterase 활성부위의 구조특성
이천배,주은희,최수라,석대은,명평근 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-
The inhibition pattern of three inhibitors(tacrine, decamethonium and propidium) on the hydrolysis of various thiocholine ester substrates by eel acetylcholinesterase was comparatively examined. When the substrate was acetylthiocholine, it showed a similar competitive inhibition by tacrine inhibitor, and a mixed type inhibition by decamethonium and propidium inhibitors. When the substrate was pentanoylthiocholine, it showed an uncompetitive inhibition by tacrine, and a noncompetitive inhibition by decamethonium. When the substrate was laurylthiocholine, it showed mixed type and uncompetitive inhibition by tacrine, and a competitive inhibition by decamethonium and propidium. Those results suggest that the active of acetylcholinesterase has the existence of hydorphobic site besides the anionic and esteratic site.
Thiocholine ester 기질을 이용한 Acetylcholinesterase 활성부위의 구조특성
이천배,주은희,최수라,석대은,명평근 충남대학교 기초과학연구소 1999 忠南科學硏究誌 Vol.26 No.1
The inhibition pattern of three inhibitors(tacrine, decamethonium and propidium) on the hydrolysis of various thiocholine ester substrates by eel acetylcholinesterase was comparatively examined. When the substrate was acetylthiocholine, it showed a similar competitive inhibition by tacrine inhibitor, and a mixed type inhibition by decamethonium and propidium inhibitors. When the substrate was pentanoylthiocholine, it showed an uncompetitive inhibition by tacrine, and a noncompetitive inhibition by decamethonium. When the substrate was laurylthiocholine, it showed mixed type and uncompetitive inhibition by tacrine, and a competitive inhibition by decamethonium and propidium. Those results suggest that the active site of acetylcholinesterase has the existence of hydrophobic site besides the anionic and esteratic site.
Su-Hwan Lee,Hui-Su Bae,Yang-Yeol Oh,Sang-Hun Lee,Yeong-Joo Kim,Sun Kim,Jin-Hee Ryu,Kang-Ho Jung,Choong-Geun Lee,Jae-Hyeon Kim,Yeong-Doo Kim,Weon-Young Choi,Jae-Yeong Cho,Kyoung-Bo Lee,Keon-Hui Lee,Ki- 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6
It is known that the poor soil fertility of newly reclaimed saline soils is due to the lack of organic matter and available mineral nutrients for crop production. The effect of green manuring with Sesbania aculeata in combination with five rates of urea-N treatments (N0. N25, N50, N75, N100) on the productivity of a subsequent whole-crop barley and the fertility of the reclaimed saline soil in Saemangeum was evaluated in the field during 2013-2014 growing season. Sesbania was grown during summer season (June to October). The amount of Sesbania incorporated was 16.2 Mg ha<SUP>-1</SUP>. Sesbania contributed to 393 kg N ha<SUP>-1</SUP> to the soils when ploughed down and incorporated before whole-crop barley cultivated. The performances of whole-crop barley following sesbania incorporation were significantly affected by a combination of Sesbania manuring and different N rates. The N fertilizer equivalence without N fertilizer following Sesbania was 42.6% (63.9 kgN ha<SUP>-1</SUP>), compared with N100(150 kg N ha<SUP>-1</SUP>) in fallow soils. The whole-crop barley yield responded to N fertilizer rates in both sesbaniaamended and fallow soil. The yield response to nitrogen rates in fallow soil was linear (Y=0.0586X+3.3011, R<SUP>2</SUP> =0.9534), whereas that in sesbania-amended soils was quadratic (Y= -0.001X<SUP>2</SUP>+0.1322X+5.7143, R<SUP>2</SUP>=0.9576). The yield of whole-crop barley in sesbania-amended with increasing N rates was increased up to SN75 (115 kgN) 10.3 Mg ha<SUP>-1</SUP>. Apparent N recovery (ANR) of whole-crop barely showed decreased with sesbania plus increasing rates of N fertilizer. Despite higher yield with sesbania manuring plus increasing N rates, the contributions of N from Sesbania with increasing N rates to whole-crop barley were decreased, whereas those from fertilizer increment due to excessively mineralized Nitrogen. Considering yield, ANR, N contribution from Sesbania and nitrogen fertilizer, the optimum N rate was N50 rate following sesbania incorporation.
Relationship between Soil Chemical Properties and Algae Growth at Rice Paddy Field
Hui Su Bae,Seung Hyeon Ahn,Jaebok Hwang,Hyeonsoo Jang,Jong Tak Youn,Uk Han Kim,Tae Seon Park,Beomsoo Bae 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4
Influences of various soil chemical properties on the algae growth (Chl-a concentration) at the paddy field were statistically analyzed using soil and water samples collected from 2016 to 2018 in June. As a result of correlation analysis between soil chemical properties and Chl-a concentration, significantly positive relationships were observed in pH, EC and SiO₂. To identify the key soil chemistry variables and predict Chl-a concentration, factor analysis was done and three principal components (PCs) with eigenvalues > 1 were found, which accounting for 60.9% of the original variance. The PC1 included Mg<SUP>2+</SUP>, Ca<SUP>2+</SUP> and Avali.-P. The PC2 included pH, Na<SUP>+</SUP>, K<SUP>+</SUP>, and Avail.-SiO₂. PC3 included OM and T-N. According to the result of linear regression analysis based on the factors by factor analysis, the total explanation amount was 18.0% and only PC3 had an significant influence on the decrease of Chl-a concentration. These result will be used for soil management to prevent algae blooms at rice paddy field.
Su-hwan Lee,Pyeong Shin,Hui-su Bae,Jang-Hee Lee,Yang-Yeol Oh,Sang-Hun Lee,Tae-Hwan Rho,Beom-Heon Song,Jae-Yeong Cho,Kyoung-Bo Lee,Keon-Hui Lee,Ki-Hoon Park 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6
Newly reclaimed tidal land is known to be in low status of soil fertility. The incorporation of crop residue is an effective method to improve soil properties and fertility in reclaimed saline soils. The objective of this study was to evaluate the efficiency of rice straw (RS) application to improve physico-chemical properties of saline-sodic soil and its contribution to productivity of whole crop barley. Increasing rate of rice straw improved growth parameter related to yield of whole crop barley, which increased tiller number significantly (p<0.05).The yield increased by 15% (F.W) and 9% (D.W) in rice straw-amended plots. The content of soil organic matter (SOM) in the surface soil (0-20cm) with rice straw incorporation increased by 5~9% (RS 2.5~RS 7.5) compared to RS 0, in which the content of SOM decreased after two consecutive cultivations. Rice straw incorporation promoted soil physico-chemical properties and nutrient-availability of the test crop, as indicated in change in soil bulk density, porosity and increased nutrient uptake of plant. Especially, the P content and uptake of whole crop barley increased with increasing the rate of rice straw application. In conclusion, the rice straw application at rates of 5.0-7.5 ton·ha<SUP>-1</SUP> in reclaimed saline soils effectively improved soil properties and crop productivity, which has potentials to reduce the loss of chemical fertilizers and facilitate the favorable condition for crop growth under adverse soil condition.
Managing Soil Organic Matter and Salinity by Crop Cultivation in Saemangeum Reclaimed Tidal Land
Bae, Hui Su,Jang, Hyeonsoo,Hwang, Jae Bok,Park, Tae Seon,Lee, Kyo Suk,Lee, Dong Sung,Chung, Doug Young Korean Society of Soil Science and Fertilizer 2018 한국토양비료학회지 Vol.51 No.1
This study was to evaluate the effect of organic amendments incorporation on soil properties and plant growth under two different soil salinity levels and various cultivated crops at Saemangeum reclaimed tidal land for three years from 2012 to 2014. The soil texture of the experimental site was sandy loam. Four different crops, sesbania (Sesbania grandiflora), sorghum-sudangrass hybrid (Sorghum bicolor-Sorghum sudanense), rice (Oryza sativa L.) and barley (Hordeum vulgare) were cultivated at low (< $1dS\;m^{-1}$) and high (> $4dS\;m^{-1}$) soil salinity levels. The soil salinity was significantly lowered at the rice cultivation site compared to continuous upland crops cultivation site in high soil salinity level. But the soil salinity was increased as cultivating sesbania coutinuously in low soil salinity level. The soil organic matter content was increased with the incorporation of straw at the continuous site of rice and barley, and the average of soil organic matter was increased by $0.9g\;kg^{-1}$ per year which was effective in soil aggregate formation. The highest biomass yield plot was found in barley (high salinity level) and sesbania (low salinity level) cultivation site, respectively. Our research indicates that rice cultivation in paddy field with high salinity level was effective in lowering soil salinity and sesbania cultivation was useful to biomass production at upland with low salinity. In conclusion, soil salinity and organic matter content should be considered for multiple land use in newly reclaimed tidal land.
Hui Su Bae,Hyeonsoo Jang,Seung Hyeon Ahn,Uk Han Kim,Jong Tak Youn,Doug Young Chung 한국토양비료학회 2021 한국토양비료학회지 Vol.54 No.2
Italian ryegrass (Lolium multiflorum Lam.) was cultivated in a field experiment, on sandy loam soil of the Saemangeum reclaimed land, under different sowing rates and N fertilizer levels to estimate the optimum N fertilizer requirement and the most suitable sowing rate for seed production. The experiment was conducted in a split-plot design with four replications. The main-plots were four sowing rates of 10, 20, 30, and 40 ㎏ ㏊<SUP>-1</SUP> and sub-plots were five N fertilizer application rates of 0, 45, 90, 135, and 180 ㎏ ㏊<SUP>-1</SUP>. Harvest index decreased with increasing sowing and N fertilizer rate. The highest N use efficiency was obtained at 10 ㎏ ㏊<SUP>-1</SUP> sowing rate and 45 - 90 ㎏ ㏊<SUP>-1</SUP> N fertilizer application rates. Maximum seed yield of Italian ryegrass based on the regression curve of N fertilizer application level was 102 ㎏ ㏊<SUP>-1</SUP> and was decreased with further N application. Economically, optimum rates of N application ranged from 70 to 101 ㎏ ㏊<SUP>-1</SUP> depending on the sowing rate. Results from this experiment, could therefore inform producers of the best combination of sowing and N fertilizer rates needed to increase profitability of Italian ryegrass seed production alongside preventing N loss. However, further research will be necessary to elucidate on increasing seed quality and stability of cultivation under various environmental conditions.