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Thi Binh Nguyen Nguyen,Thi Kieu Diem Nguyen,Van Hue Trương,Thi Tuyet Ngoc Tran,van Bao Thang Phan,Thi Tuyen Nguyen,Hoang Bach Nguyen,Viet Quynh Tram Ngo,Van Tuan Mai,Paola Molicotti 질병관리본부 2023 Osong Public Health and Research Persptectives Vol.14 No.5
Objectives: Tuberculosis (TB) and drug-resistant TB (DR-TB) are national health burdens in Vietnam. In this study, we investigated the prevalence of rifampicin (RIF) and/or isoniazid (isonicotinic acid hydrazide, INH) resistance in patients with suspected TB, and applied appropriate techniques to help rapidly target DR-TB. Methods: In total, 1,547 clinical specimens were collected and cultured using the BACTEC MGIT system (Becton Dickinson and Co.). A resazurin microtiter assay (REMA) was used to determine the proportions of RIF and/or INH resistance. A real-time polymerase chain reaction panel with TaqMan probes was employed to identify the mutations of rpoB and katG associated with DR-TB in clinical isolates. Genotyping of the identified mutations was also performed. Results: A total of 468 Mycobacterium tuberculosis isolates were identified using the REMA. Of these isolates, 106 (22.6%) were found to be resistant to 1 or both antibiotics. Of the resistant isolates, 74 isolates (69.8%) were resistant to isoniazid (INH) only, while 1 isolate (0.94%) was resistant to RIF only. Notably, 31 isolates (29.24%) were resistant to both antibiotics. Of the 41 phenotypically INH-resistant isolates, 19 (46.3%) had the Ser315Thr mutation. There were 8 different rpoB mutations in 22 (68.8%) of the RIF-resistant isolates. The most frequently detected mutations were at codons 531 (37.5%), 526 (18.8%), and 516 (6.3%). Conclusion: To help prevent new cases of DR-TB in Vietnam, it is crucial to gain a comprehensive understanding of the genotypic DR-TB isolates.
Tung Nguyen-Thach,Nguyen Canh-Hung,Nguyen Van-Duong,Nguyen Thi-Hong-Thuy,Nguyen Van-Lam,Tran Cao-Son,Pham Thi-Minh-Hue 한국약제학회 2020 Journal of Pharmaceutical Investigation Vol.50 No.4
Purpose The study aimed firstly to determine the release behavior of the model drug (berberine chloride) from the dry coated tablets. The second objective of this study was to evaluate the exact location of the dry coated tablets in in vivo. Methods The colon targeting tablets were developed by dry powder coating technique on pan coater. The drug release behavior was determined in the three continuous mediums: pH 1.2; 7.4 and 6.8 plus pectinase. The location of the dry coated tablets in the gastrointestinal tract of human volunteers was observed through the X-ray imaging of the dry coated tablets containing the optimized radiocontrast agents. Results The release kinetics of berberine chloride from the dry coated tablets was mainly controlled by erosion and enzyme sensitive mechanism. The optimum dry coated tablets having the coating powders of pectin 102:HPMC K4 M (2:1) with the coating level of 200%, and the tablet core with BaSO4 10% and iobitridol 30% as radiocontrast agents were observed in the caecum and ascending colon of human volunteers after 5–6 h of oral administration. Conclusion The successful development of these dosage forms is believed to have a high potential in precisely monitoring the release of highly potent drugs such as anti-inflammatory drugs in bowel diseases.
Close-Packed Monolayer Self-Assembly of Silica Nanospheres Assisted by Infrared Irradiation
Nguyen Van Minh,Nguyen Thi Hue,Nghiem Thi Ha Lien,Chu Manh Hoang 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.1
In this paper, we report on a fast and cost-effective dropcoating technique for the self-assembly of silica nano-spheresfrom a mono-dispersed colloidal suspension into close-packedmonolayer (CMP) on hydrophilic single-crystal siliconsubstrate. The technique includes the self-assembly of silicanano-spheres on slanted silicon substrate and infraredirradiation during evaporation process of the coated droplet. The influence of the substrate slant angle and infraredirradiation on the formation of silica nano-sphere monolayer isinvestigated. This achievement is promising for variousapplications, such as a mask layer for nano-sphere lithographythat is employed for producing fundamental elements inphotonics, plasmonics, and solar cell.
Hue, Nguyen Thi Ngoc,Derva Jarvis,Bhuwon Sthapit 한국생태학회 2004 Journal of Ecology and Environment Vol.27 No.1
Understanding farmer household and community management of crop diversity is necessary in order to effectively strengthen the local plant genetic resources management system in Vietnam. Assessment and management for distribution of farmer s cultivars of rice, taro and tropical tree species were conducted in four different agro-ecological zones in Vietnam using participatory approaches, focus surveys, diversity fairs, agro-morphological field trials, isozyme analysis to understand why Vietnamese farmers grow diverse types of these crop cultivars, when and where these crops cultivars are grown and how farmers maintain and use them. Results showed significant differences in the extent, distribution and use pattern crop cultivars diversity in contrasting environments and between community farmer households. Seed system is an important role in the access and use of diversity in eco-geographical regions.
Nguyen Thi Hue,Anaya Raj Pokhrel,Nguyen Thanh Chung,Jae Kyung Sohng 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Drug development research requires a large amount of target proteins. Screening of drug target often requires 13C- and 15N- labeled protein, and higher protein expression has great advantages for obtaining isotope-labeled proteins. Among many of the proteins, glycosyltransferases(GTs) are attractive biocatalysts in producing a series of important bioactive natural products. In case of kanamycin, kanF gene encodes the first glycosyltransferase which acts both as glucosyltransferase and N-acetyl-glucosaminyl-transferase in kanamycin biosynthetic pathway of Streptomyces kanamyceticus. The recombinant expression of kanF gene in E. coli BL21 (DE3), BL21pLysS and BL21-CodonPlus® (DE3)-RP under T7 promoter-based system showed the expression in insoluble rather than soluble form. Further analysis of the codons revealed that this gene includes number of rare codons which are difficult to be translated in E. coli. Due to variations in codon usage between E. coli and Streptomyces, with high free energy of secondary structure of mRNA, KanF was not expressed under various condition tested. Thus, using codon optimized gene based on codon usage of E. coli has helped to overcome this problem and led to soluble expression of protein. Thus obtained KanF was used for making in vitro reaction for glycosylation of 2-DOS and the product was confirmed to be 2′ -Deamino-2′-hydroxyparomamine by high resolution Q-TOF mass analysis.
The Relationship Between Chromium Content and Erosion-Corrosion Resistance of Fe-Cr-C Alloy System
Hue, Nguyen Viet,Phuong, Doan Dinh,Tich, Nguyen Van,Binh, Hoang Thi 한국부식방식학회 2002 Corrosion Science and Technology Vol.31 No.1
In order to determine the influence of chromium on erosian-comsian coefficient of alloy in acid media, experiments were performed with chromium content in the range of 12-33% in different typical erosion-corrosion environments such as solution of sand, solution of sand with free acid in pH 2-7. The erosion-corrosion coefficient was evaluated by Apparatus for abrasion-corrosion testing. Wear coetr-icient, K, was calculated by formula: K=ΔMetalon/ΔMalloy Testing results were showed that the alloy with composition (%): Cr=28-30; C=1.8-2.0; Mn=2.5 - 3.0; is the optimum for manufacturing details resistant erosion-corrosion in media upto pH=2-3. This alloy is used successfully to produce details for sand-pumps, minerals processing cyclones.
Biosynthesis of fucosyllactose in engineered Corynebacterium glutamicum
Hue Thi Nguyen,Obed Jackson Amoah,Su Yeong Ma,Jae Kyung Sohng 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Human milk oligosaccharides (HMOs) have been proven to be a key ingredient found in breast milk and beneficial to infant health. Structurally, the fucose-containing oligosaccharides are major components, with a proportion of approximately 50–80% in the HMOs. In particular, 2-fucosyllactose (2’-FL) and 3-fucosyllactose (3-FL) are 2 major components of the abundant HMO component. Importantly, 2’-FL is approved for use in infant formula for improving probiotic functions by the FDA of the USA and the European Food Safety Authority, while 3-FL can be used as a prebiotic to improve the growth of beneficial microorganisms. Over the last decade, various approaches have been conducted for fucosyllactose (FL) production, including chemical synthesis, enzymatic conversion, and microbial fermentation. Most studies were performed in Escherichia coli due to the simple culture conditions and availability of various genetic tools. Besides E. coli, Bacillus subtilis and Saccharomyces cerevisiae have also been selected as the hosts for the production of FL due to their generally regarded safe (GRAS) status. As a type of GRAS strain, Corynebacterium glutamicum has been widely used in the biosynthesis of food-grade, high-value-added products, including lysine, glutamic acid, violacein and resveratrol. In this study, the biosynthetic enzymes of FL were condon-optimized and heterologously expressed in engineered C. glutamicum for the production of FL. The trace amount production of FL was confirmed by TLC, HPLC in this study.