Indoor feeding combined with restricted grazing time improves body health, slaughter performance, and meat quality in Huang-huai sheep

Huang Yafeng,Zhao Mengyu,Zhang Xiaoan,Wei Huiqing,Liu Lumeng,Zhang Zi Jun,Cheng Xiao,Wang Guanjun,Ren Chunhuan 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.11

Objective: The aim of this study was to evaluate the effects of three feeding systems, i.e., indoor feeding (CON), indoor feeding with 4-h daily access to grazing artificial pasture (ITGP), and indoor feeding with 8-h daily access to grazing artificial pasture (IEGP), on the plasma antioxidant and immunological capacity, slaughter characteristics, meat quality and economic efficiency of Huang-huai lambs. Methods: Thirty-three healthy Huang-huai rams with similar body weight (approximately 5 mo of age, 28.96±1.01 kg) were assigned equally to three experimental groups. When finished fattening, six lambs from each group were collect blood samples for plasma analyses and then slaughtered to determine slaughter characteristics and obtain biceps brachii muscle for further analysis of meat quality and fatty acid profile. Results: Compared to CON group, animals submitted to ITGP and IEGP groups resulted in greater contents of serum glutathione peroxidase, immunoglobulins (IgA, IgG, and IgM), polyunsaturated fatty acids (PUFA), n-6 PUFA, and PUFA/saturated fatty acid (FA) ratio and lower palmitic /oleic acid ratio (p<0.05). Moreover, animals in ITGP group exhibited a higher (p<0.05) loin eye area, content of meat crude protein (CP), and eicosetrienoic acid compared to CON group, while slaughter performance was superior (p<0.05) to that of the IEGP group. The economic efficiency of ITGP group was 70.12% higher than that of CON group, while the IEGP group exhibited a decrease of 92.54% in economic efficiency compared to the CON group. Conclusion: Restricted grazing time combined with indoor feeding was more effective in conferring superior body health, carcass traits and economic efficiency in Huang-huai lambs, as well as higher CP content and healthier FA composition in the resulting meat.

COMPLETELY GENERALIZED MILDLY NONLINEAR COMPLEMENTARITY PROBLEMS FOR FUZZY MAPPINGS

Huang, Nan-Jing,Zhang, Wen-Bin 한국전산응용수학회 1997 Journal of applied mathematics & informatics Vol.4 No.2

In this paper we introduce and study a new class of completely generalized mildly nonlinear complementarity problems for fuzzy mappings and construct some new iterative algorithms. We also show the existence of solution and the convergence of iterative sequences generated by the algorithm. Our results extend some recent results of Noor, Chang and huang.

Investigation of Quench Sensitivity and Microstructure Evolution During Isothermal Treatment in 2195 Al–Li Alloy

Zhiwu Zhang,Youping Yi,Wen You,Shiquan Huang,Yonglin Guo,Hailin He 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

To investigate the quenching sensitivity of the 2195 Al–Li alloy rolled sheet and guide the design of the quenching process,the time–temperature-property (TTP) curves of this material were researched through interrupted quenching experiments. The differential scanning calorimetry (DSC) and transmission electron microscope (TEM) were used to characterize theevolution of precipitates during isothermal treatment. The results of this essay demonstrated that the nose temperature of 2195Al–Li alloy is around 370 °C and the temperature range of quenching sensitivity is 340 °C to 400 °C. The microstructureobservation revealed that the T1particles precipitate and grow rapidly at the temperature from 340 to 400 °C, which is dueto the high nucleation rate of phase and fast solute diffusion kinetics, especially at the nose temperature. The needle-shapedθ′/θ″ and T1particles grow up quickly as the isothermal preservation time prolonged, leading to the decrease of the supersaturatedsolid solution of the matrix. This will reduce the number of the age-induced precipitate and weaken the subsequentage hardening effect. Therefore, the rate of cooling should be increased in the quenching sensitivity range (340–400 °C) toinhibit the precipitation of the second phase and obtain excellent mechanical properties. While in other temperature ranges,the cooling rate should be decreased appropriately to reduce residual stress. The appropriate average cooling rate is recommendedto be around 13 °C s−1 at the temperature from 340 to 400 °C.

Phosphorylation of DYNLT1 at Serine 82 Regulates Microtubule Stability and Mitochondrial Permeabilization in Hypoxia

Xue Xu,Yue-sheng Huang,Qiong Zhang,Jiong-yu Hu,Dong-xia Zhang2,Xu-pin Jiang,jie-zhi Jia,Jing-ci Zhu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Hypoxia-induced microtubule disruption and mitochondrial permeability transition (mPT) are crucial events leading to fatal cell damage and recent studies showed that microtubules (MTs) are involved in the modulation of mitochondrial function. Dynein light chain Tctex-type 1 (DYNLT1) is thought to be associated with MTs and mitochondria. Previously we demonstrated that DYNLT1 knockdown aggravates hypoxia-induced mitochondrial permeabilization, which indicates a role of DYNLT1 in hypoxic cytoprotection. But the underlying regulatory mechanism of DYNLT1 remains illusive. Here we aimed to investigate the phosphorylation alteration of DYNLT1 at serine 82 (S82) in hypoxia (1% O2). We therefore constructed recombinant adenoviruses to generate S82E and S82A mutants, used to transfect H9c2 and HeLa cell lines. Development of hypoxia-induced mPT (MMP examining, Cyt c release and mPT pore opening assay), hypoxic energy metabolism (cellular viability and ATP quantification), and stability of MTs were examined. Our results showed that phosph-S82 (S82-P) expression was increased in early hypoxia; S82E mutation (phosphomimic) aggravated mitochondrial damage, ele-vated the free tubulin in cytoplasm and decreased the cellular viability; S82A mutation (dephosphomimic) seemed to diminish the hypoxia-induced injury. These data suggest that DYNLT1 phosphorylation at S82 is involved in MTs and mitochondria regulation, and their interaction and cooperation contribute to the cellular hypoxic tolerance. Thus, we provide new insights into a DYNLT1 mechanism in stabilizing MTs and mitochondria, and propose a potential therapeutic target for hypoxia cytoprotective studies.

Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture

Wei Wang,Zhen Zhang,Yinsheng Guo,Yu Dong,Xiaoyu Huang1,Yijun Zhou,Guoyuan Chen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Carbon disulfide (CS2), a common organic solvent, induces a variety of adverse effects in the male reproductive system. In this study, we investigated the cytotoxicity, ultrastructural changes, and potential apoptotic induction mechanisms of CS2 in mixed cultures of Sertoli and germ cells. Sertoli and germ cells were cocultured and treated with CS2 for 24 h. Growth rates were noted, and apoptotic cells were identified by Hoechst 33258 staining. Ultrastructure changes were observed via transmission electron microscopy (TEM). Mitochondrial membrane potential and expressions of apoptosis-related factors (cytochrome c, Bax, Bcl- 2, caspase-3 and caspase-9) were examined by JC-1 staining, western blot, and real-time PCR. The results showed that CS2 treatment was associated with reduced growth rates of Sertoli-germ cells. Ultrastructure changes in Sertoli-germ cells treated with CS2 were typical of apoptosis. In addition, CS2 treatment depolarized mitochondrial membrane potential, upregulated Bax levels and downregulated Bcl-2 levels, released cytochrome c from the mitochondrial intermembrane space to the cytosol, and triggered mitochondria-mediated apoptosis. Subsequently, caspase-9 and caspase-3 were activated, resulting in Sertoli-germ cells apoptosis. The above data suggest that CS2 has adverse effect on the viability of Sertoli-germ cells and induces apoptosis through mitochondrial pathway.

piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.

Effects of Vitamin D Supplementation on Children with Autism Spectrum Disorder: A Systematic Review and Meta-analysis

Min Zhang(Min Zhang),YiRan Wu(YiRan Wu),ZhaoXu Lu(ZhaoXu Lu),MeiYan Song(MeiYan Song),XiaoLan Huang(XiaoLan Huang),LaLa Mi(LaLa Mi),Jian Yang(Jian Yang),Xiaodai Cui(Xiaodai Cui) 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.2

The effect of vitamin D supplementation on individuals with autism spectrum disorder (ASD) is inconclusive. We aimed to conduct a meta-analysis of the available randomized controlled trials (RCTs) to explore whether vitamin D supplementation can improve core symptoms and coexisting conditions in children with ASD. Data were obtained by searching the PubMed, Embase, Web of Science, CINAHL and Cochrane Library databases up to February 2022 following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Using a random-effects model, mean differences with 95% confidence intervals (CIs) were calculated through a meta-analysis. There were eight RCTs with 266 children with ASD in the present review, among which six RCTs were included in the meta-analysis. Children who received vitamin D supplementation showed a significant improvement in stereotypical behavior scores (pooled mean difference (MD): −1.39; 95% CI: −2.7, −0.07; p = 0.04) with low heterogeneity (I2 = 34%), and there was a trend toward decreased total scores on the Social Responsiveness Scale (SRS) and Childhood Autism Rating Scale (CARS, p = 0.05); however, there were no other significant differences in the core symptoms of ASD and coexisting conditions between groups as measured by the Aberrant Behavior Checklist (ABC). Vitamin D supplementation appears to improve stereotypical behaviors but does not improve other core symptoms and coexisting conditions. Further randomized controlled trials with large sample sizes and individualized doses are needed.

Insights Into the BR2/PGP1-mediated Patterns for Shoot and Root Growth in Maize Early Seedling Development by Comparative Transcriptome Sequencing

Xiangge Zhang,Lanjie Zheng,Haojun Zhang,Xinrong Huang,Yinghong Liu,Junjie Zhang,Yu-Feng Hu,Hanmei Liu,Guowu Yu,Yangping Li,Yubi Huang 한국식물학회 2019 Journal of Plant Biology Vol.62 No.3

Plant shoot and root growth is regulated by multiplefactors, including the hormone auxin. A maize brachytic2(br2) mutant displays a shortened shoot and root with highauxin concentration in their apexes, where BR2 encodes anauxin transporter P-glycoprotein1 (PGP1). We employedcomparative transcriptome sequencing to explore the BR2/PGP1-mediated patterns for shoot and root growth amongbr2, wild type (WT), and hybrid F1 (br2 × WT). The resultsshow 352 genes in the br2 shoot and 234 genes in the br2root are differentially expressed compared to WT and F1. Zm00001d045203 and Zm00001d031562 were identified astwo auxin-responsive genes in both the shoot and root. TheZm00001d045203 gene is down-regulated in br2 and encodesan Aux/IAA inhibitor involved in the SCFTIR1/AFB-mediatedauxin signaling pathway. The up-regulated Zm00001d031562gene encodes a small GTPase belonging to the Rab/Yptfamily, which takes part in auxin-mediated responses inArabidopsis. Cytology analysis reveals that reduced cellelongation affects the shoot and root growth of br2. TheZm00001d043477 gene is down-regulated and encodes amember of the cellulose synthase protein family, possiblyreducing cell elongation in the br2 shoot and root. Theseresults reveal the specific regulation of auxin actions and cellresponses by BR2/PGP1.

Exploring the Impact of Live Commercial Anchors on BusinessPerformance and Survival Strategy: Focusing on Resource Configuration and Environmental Choice 1

Li-Jie ZHANG(Li-Jie ZHANG),Fei HUANG(Fei HUANG) 동아시아경상학회 2022 The East Asian Journal of Business Economics Vol.10 No.4

Purpose – Through the analysis and research of different characteristics and related mechanisms of live commerce anchors, this paper aimed to help live commerce anchors to strengthen their characteristic advantages, beautify the live broadcast environment, improve resource allocation and enhance business performance, so as to help live commerce anchors to achieve better high-quality development. Research design, data, and methodology – This study was conducted by sample survey method on 361 live commercial anchors who have experience in using major online shopping malls for more than one year. Data analysis methods were frequency analysis, confirmatory factor analysis, reliability analysis, correlation analysis, and structural equation model analysis. Result – Hypothesis 1 through Hypothesis 5 were all supported. The results of this study suggest that professionalism and mutual entertainment of live commercial anchors have a significant impact on business performance, survival strategy, resource allocation and environment selection. The resource allocation and environment selection of live commercial anchors play a mediating role in the professionalism and mutual entertainment of live commercial anchors on business performance and survival strategy. Conclusion –The results implied that the professionalism and inter entertainment of live commercial anchors have a positive impact on business performance and survival strategy. In the new situation, live commercial anchors should further improve their professionalism and mutual entertainment, so as to achieve better development.

A red-emitting phosphor of Li₅La₃Ti₂O₁₂:Eu³⁺ with garnet-like structure and near-UV/blue light excitation

Zhang, Suyin,Zhang, Pengyue,Liu, Xin,Yang, Zhexi,Huang, Yanlin,Seo, Hyo Jin Elsevier 2018 Journal of luminescence Vol.203 No.-

<P><B>Abstract</B></P> <P>A series of novel red-emitting phosphor of Li<SUB>5</SUB>La<SUB>3–3x</SUB>Eu<SUB>3x</SUB>Ti<SUB>2</SUB>O<SUB>12</SUB> (x = 0.01–0.25) were synthesized via the facile solid-state reaction method. The Rietveld refinements verified that all the samples crystallized in a garnet-like cubic structure. The band gap energy of Li<SUB>5</SUB>La<SUB>3</SUB>Ta<SUB>2</SUB>O<SUB>12</SUB> is 3.87 eV, which can be greatly narrowed by Eu<SUP>3+</SUP>-doping. This benefits the red-shift of optical absorption from UV- to near-UV light region. Importantly, the sample presents dominant absorption in near-UV (390–395 nm) and blue-light (460–465 nm) region. Li<SUB>5</SUB>La<SUB>3–3x</SUB>Eu<SUB>3x</SUB>Ta<SUB>2</SUB>O<SUB>12</SUB> (x = 0.01–0.15) has a bright red-luminescence with the strongest <SUP>5</SUP>D<SUB>0</SUB> → <SUP>7</SUP>F<SUB>2</SUB> transition at 613 nm. The energy transfer was discussed on the Eu<SUP>3+</SUP>-doping concentration. The experimental red-emitting lamps were fabricated using a 395 and a 460 nm LED-chips, respectively, in combination with the single phosphor of Li<SUB>5</SUB>La<SUB>3–3x</SUB>Eu<SUB>3x</SUB>Ta<SUB>2</SUB>O<SUB>12</SUB> (x = 0.2) driven by 2 mA current. The results indicate that the phosphor could be a promising candidate used in solid-state lightings and displays.</P>