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Fu, Qiang,Wang, Bai-Chen,Jin, Xiang,Li, Hong-Bing,Han, Pei,Wei, Kai-Hua,Zhang, Xue-Min,Zhu, Yu-Xian Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6
Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3% of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.
( Bo Xu ),( Fu Ya Yang ),( Cai Yun Xiong ),( Jun Jun Li ),( Xiang Hua Tang ),( Jun Pei Zhou ),( Zhen Rong Xie ),( Jun Mei Ding ),( Yun Juan Yang ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-amylase. AmyPL was expressed in Escherichia coli BL21 (DE3) and the purified AmyPL was enzymatically characterized. This study is the first to report the molecular and biochemical characterization of a novel α-amylase from a gastrointestinal metagenomic library.
Zhu, Hong-Hu,Liu, Lin-Chao,Pei, Hua-Fu,Shi, Bin Techno-Press 2012 Geomechanics & engineering Vol.4 No.1
Soil foundations exhibit significant creeping deformation, which may result in excessive settlement and failure of superstructures. Based on the theory of viscoelasticity and fractional calculus, a fractional Kelvin-Voigt model is proposed to account for the time-dependent behavior of soil foundation under vertical line load. Analytical solution of settlements in the foundation was derived using Laplace transforms. The influence of the model parameters on the time-dependent settlement is studied through a parametric study. Results indicate that the settlement-time relationship can be accurately captured by varying values of the fractional order of differential operator and the coefficient of viscosity. In comparison with the classical Kelvin-Voigt model, the fractional model can provide a more accurate prediction of long-term settlements of soil foundation. The determination of influential distance also affects the calculation of settlements.
An optical fibre monitoring system for evaluating the performance of a soil nailed slope
Zhu, Hong-Hu,Ho, Albert N.L.,Yin, Jian-Hua,Sun, H.W.,Pei, Hua-Fu,Hong, Cheng-Yu Techno-Press 2012 Smart Structures and Systems, An International Jou Vol.9 No.5
Conventional geotechnical instrumentation techniques available for monitoring of slopes, especially soil-nailed slopes have limitations such as electromagnetic interference, low accuracy, poor longterm reliability and difficulty in mounting a series of strain sensors on a soil nail bar with a small-diameter. This paper presents a slope monitoring system based on fibre Bragg grating (FBG) sensing technology. This monitoring system is designed to perform long-term monitoring of slope movements, strains along soil nails, and other slope reinforcement elements. All these FBG sensors are fabricated and calibrated in laboratory and a trial of this monitoring system has been successfully conducted on a roadside slope in Hong Kong. As part of the slope stability improvement works, soil nails and a toe support soldier-pile wall were constructed. During the slope works, more than 100 FBG sensors were installed on a soil nail, a soldier pile, and an in- place inclinometer. The paper presents the layout and arrangement of the instruments as well as the installation procedures adopted. Monitoring data have been collected since March 2008. This trial has demonstrated the great potential of the optical fibre monitoring system for long-term monitoring of slope performance. The advantages of the slope monitoring system and experience gained in the field implementation are also discussed in the paper.
An optical fibre monitoring system for evaluating the performance of a soil nailed slope
Hong-Hu Zhu,Albert N.L. Ho,Jian-Hua Yin,H.W. Sun,Hua-Fu Pei,Cheng-Yu Hong 국제구조공학회 2012 Smart Structures and Systems, An International Jou Vol.9 No.5
Conventional geotechnical instrumentation techniques available for monitoring of slopes, especially soil-nailed slopes have limitations such as electromagnetic interference, low accuracy, poor longterm reliability and difficulty in mounting a series of strain sensors on a soil nail bar with a small-diameter. This paper presents a slope monitoring system based on fibre Bragg grating (FBG) sensing technology. This monitoring system is designed to perform long-term monitoring of slope movements, strains along soil nails, and other slope reinforcement elements. All these FBG sensors are fabricated and calibrated in laboratory and a trial of this monitoring system has been successfully conducted on a roadside slope in Hong Kong. As part of the slope stability improvement works, soil nails and a toe support soldier-pile wall were constructed. During the slope works, more than 100 FBG sensors were installed on a soil nail, a soldier pile, and an in- place inclinometer. The paper presents the layout and arrangement of the instruments as well as the installation procedures adopted. Monitoring data have been collected since March 2008. This trial has demonstrated the great potential of the optical fibre monitoring system for long-term monitoring of slope performance. The advantages of the slope monitoring system and experience gained in the field implementation are also discussed in the paper.