Purification and Characterization of an Extracellular Protease from Bacillus pumilus CN8

Mei-Hu Ma,Yong-Guo Jin,Hao-Li Li,Jun Wang,Ha-Na Kim,오덕환 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.1

The protease produced by a Bacillus pumilus CN8 strain was purified by DEAE-Cellulose-52 ion exchange. It has a molecular weight of approximately 96,920 Dalton. In the present study, this protease showed strong activity over a broad range of pH (6.5-9.5) and temperature from 40℃ to 60℃, and the protease performed the maximal activity at pH 7.3 at 42℃. The effect of metal ions on protease activity showed that K+ could slightly increase the protease activity, and other ions such as Zn²+, Fe²+, Na+, Ca²+, Mg²+ had no significant activation or inhibition to the protease (P > 0.05), and the more important is that Cu²+, Mn²+, Sn²+, Cd²+ had a strong inhibitory effect on the protease activity.

A Multi-center Survey of HPV Knowledge and Attitudes Toward HPV Vaccination among Women, Government Officials, and Medical Personnel in China

Zhao, Fang-Hui,Tiggelaar, Sarah M.,Hu, Shang-Ying,Zhao, Na,Hong, Ying,Niyazi, Mayinuer,Gao, Xiao-Hong,Ju, Li-Rong,Zhang, Li-Qin,Feng, Xiang-Xian,Duan, Xian-Zhi,Song, Xiu-Ling,Wang, Jing,Yang, Yun,Li, Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objectives: To assess knowledge of HPV and attitudes towards HPV vaccination among the general female population, government officials, and healthcare providers in China to assist the development of an effective national HPV vaccination program. Methods: A cross-sectional epidemiologic survey was conducted across 21 urban and rural sites in China using a short questionnaire. 763 government officials, 760 healthcare providers, and 11,681 women aged 15-59 years were included in the final analysis. Data were analyzed using standard descriptive statistics and logistic regression. Results: Knowledge of HPV among the general female population was low; only 24% had heard of HPV. Less than 20% of healthcare providers recognized sexually na$\ddot{i}$ve women as the most appropriate population for HPV vaccination. There was high acceptance of the HPV vaccine for all categories of respondents. Only 6% of women were willing to pay more than US $300 for the vaccine. Conclusions: Aggressive education is necessary to increase knowledge of HPV and its vaccine. Further proof of vaccine safety and efficacy and government subsidies combined with increased awareness could facilitate development and implementation of HPV vaccination in China.

Innovative simulation method of the spherical steel bearing applied to high-speed railway bridges

Renkang Hu,Shangtao Hu,Xiaoyu Zhang,Menggang Yang,Na Zheng 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.85 No.2

The spherical steel bearings (SSBs) has been gradually replaced traditional rubber bearings and extensively applied to high-speed railway (HSR) bridges in China, due to their durability and serviceability. Nevertheless, SSB is generally simplified to the ordinary constraints in the finite element model, which cannot reflect its detailed mechanical characteristics, especially its seismic performance. To provide a more precisely simulation, an innovative and simplified finite element simulation method is proposed and the combined element group is developed in ANSYS. The primary parameters were determined by means of the performance test of SSB. The finite element model of SSB applied to a single-span HSR simply supported girder bridge was established through the proposed method. The seismic performance of the SSB was further investigated. A shake table test was conducted to evaluate the accuracy of the proposed simulation method. It is found that the numerical results could have a good agreement with the experiment, namely, the proposed method is feasible and efficient.

晚唐士族女婚姻生活的個案研究

胡娜(HU Na) 경북대학교 아시아연구소 2014 아시아연구 Vol.- No.19

현재 당대 묘지를 활용하여 당대 여성을 연구한 성과가 상당히 많이 있다. 그러나 대다수는 당대 여성과 사회문화 및 습속에 대한 연구로 여성주의 시각에서 당대 여성의 개체 생활 경험을 미시적으로 관찰한 사례 연구는 부족한 상황이다. 본문은 이에 기초하여 섬서 사범대학 박물관에 소장되어 있는 만당 사대부 李毗妻 盧氏 墓誌 拓片을 활용하고 李毗 墓誌와 상관된 내용을 결합하여 范陽 盧氏 여자의 혼인 생활에 대한 개별적인 사례 연구를 통해 만당 산동지역 士族 여성의 짧은 혼인 생활에서 부딪힌 경험을 분석하고자 한다. 먼저 盧李 두사람의 혼인은 중후기 당나라 때 혼인이 門第科第가 중시되었음을 볼 수 있다. 특히 李毗의 進士出身은 門第, 家境과 년령상의 부족을 보완한 것으로 盧氏가 비록 17歲이었지만 여전히 혼인을 허락받을 수 있었다. 다음으로 盧氏 婚事는 우역곡절이 있었는데, 盧氏가 22세때 李毗와 정혼할 수 있었지만 미혼부 李毗의 임직 조정과 父喪으로 혼사가 연기되었다. 26세때 盧氏는 비록 아버지가 돌아가셨지만 아버지의 유언으로 "因情廢禮"함으로 부상을 지키지 않고 늦은 나이에 출가할 수 있었다. 이외에 盧와 李의 老夫少妻의 혼인에서 나이 많은 李毗는 노씨를 취하기 전에 姬妾과의 사이에 아들을 하나 두었고, 노씨는 혼인 후 3년반 동안 두 아들을 낳은 후 불행히 30세에 병환으로 사망하였다. 노씨의 혼인 사례 연구는 몇가지 대표성이 있다. 만당 시기 사족녀 혼인중의 생존 상태를 집중적으로 반영하고 굴절시켜주고 있다. 만당시기 점차 몰락하는 고문사족의 "作嬪君子, 以援吾宗"라는 혼인 목적하에 여성의 혼인 의사가 별로 관심을 끌지 못했고 어떤 때는 가정 변고 등의 요소에 영향을 받아 혼인이 지연되거나 늦게 시집을 가기도 하였다. 老夫少妻의 혼인 관계 중 남성은 과거 급제나 사회적 지위로 나이나 모습의 부족을 보완할 수 있지만 여성은 단지 부모의 명령하에 피동적으로 老夫少妻의 婚姻을 받아들일 수 밖에 없었다. 나이든 남편은 종종 첩을 취한 후 처를 맞이하여 혼전에 姬妾과의 사이에 자녀를 생육하기도 하였는데, 이는 자주 처첩에게 嫡嗣不立의 婚姻곤경과 불안을 초래하였다. Many scholars in China have already made good use of the epitaphs in the researches on history of women in the Tang Dynasty. However, most of the researches focus mainly on women and social culture in Tang Dynasty. Scholars pay less attention to women themselves experience of individual in Tang Dynasty. There exists a lack of a case study on Tang Dynasty woman .This paper takes the late Tang Dynasty Fang-yang Lu Lady's epigraph rubbing collected in Shaanxi Normal University Museum as key point. With a combination of the message of her husband Li Pi's epigraph, this paper takes Fang-yang Lu lady's marriage life with Li Pi as a case study, aiming to analyze Fang-yang Lu lady's experience of individual in her marriage life. According to Fang-yang Lu lady's epigraph, she was from Fang-yang Lu shi family which was very famous family in Tang Dynasty. Meanwhile, according to Li Pi's epigraph, Li Pi depicted himself from Long-xi Li shi family which was another famous family in Tang Dynasty. As for their marriage, it reflected that the marriage in gentry's class was based on the family social status and also the imperial examination system exerted an important influence on the society of Tang Dynasty. That is, marriage focus on family social status as well as to what a young man had achieved in the imperial examination. In Fang-yang Lu lady's marriage, while Li Pi was 17 years older than her. The fact that Li Pi got the official career by imperial examination and his official career was very smooth gave Li Pi a big advantage for the marriage with Fang-yang Lu lady. Fang-yang Lu lady's marriage was not smooth. She should have married to Li Pi at the age of 22. Unfortunately, her wedding was delayed because of her husband's promotion and the death of his husband father. Li Pi had to be in mourning for three years for his father according to the funeral etiquette. When Fang-yang Lu lady was 26 years old, her father died. Thanks to Fang-yang Lu lady's father last wishes, Fang-yang Lu lady needn't to be in mourning for three years for her father according to the funeral etiquette, so her weeding was not delayed again. Finally, Fang-yang Lu lady married to Li Pi at her 26 years old which was considered an older age above the average age for marriage at that time. But the fact that can not be ignored was that her husband Li Pi was 17 years older than her. In this marriage of "an old man with a young wife", Fang-yang Lu lady was also faced with the fact that Li Pi took concubine and had a son before their wedding. Even more unfortunately, Fang-yang Lu lady died at the young age of 30. In the less than four years of marriage life with Li Pi, Fang-yang Lu lady gave birth to two children. Fang-yang Lu Lady's case reflected the marriage of the noble ladies in Late Tang Dynasty. Under the concept of marriage of dying gentry in late Tang Dynasty, the noble ladies' will was ignored. Sometimes, in order to get the help from another family, their family married them to the man who may be more than ten years older than them but had smooth official career. Affected by some factors, noble ladies got married above the average age for marriage at that time. In the marriage of "an old man with a young wife", faced with the fact that husband may take concubine and have children before marriage, they worried that they had no son but the concubine had already a son. On the contrary, man could have normal sexual experiences and rich feelings before marriage, and then could have a young wife. Man offset their older age by their smooth official career, but women couldn't. Men were the beneficiaries of the marriage of "an old man with a young wife". 目前學術界運用唐代墓誌研究唐代女性的成果頗多,但大多側重對唐代女性與社會文化及習俗的研究,缺少從女性主義角度對唐代女性個體生活經驗的微觀關注和個案研究,本文正是基於此,嘗試以陜西師範大學博物館藏晚唐士大夫李毗妻盧氏墓誌拓片為切入點,幷結合李毗墓誌的相關內容,對范陽盧氏女的婚姻生

An Improved LDV-Hop Localization Algorithm in Wireless Sensor Networks

Xiaoan Bao,Zhongtao Ren,Na Zhang,Xing Hu,Tongshu Wu,Yangqiu Liu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

Localization of sensor nodes is a fundamental and a key technology in wireless sensor networks, it has been widely studied by many universities and research institutions in recent years and many localization algorithms have been put forward by scholars from diverse aspects. A new localization algorithm, which is based on the LDV-Hop algorithm, is proposed in this paper. Firstly, to reduce the localization error we modify the average one-hop distance between anchor nodes, and the average one-hop distance used for each unknown node's location is also modified through weighting the received average one-hop distance from anchor nodes. Then, to solve the problem of low localization coverage which is the main disadvantage of LDV-Hop localization algorithm, we regard regular node with newly estimated location as anchor node and utilize believable factor threshold to restrain the error accumulation brought by this method.

Systemic Analysis on Laparoscope-Assisted Gastrectomy for Patients with Gastric Cancer

Hu, Jun-Hong,Tang, Hong-Na,Ma, Yong-Ping,Wang, Chen-Yu,Yao, Kun-Hou,Zhang, Jun-Jie,Ren, Xue-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: Laparoscope-assisted gastrectomy in treating patients with gastric cancers developed with a background of highly invasive traditional surgery and is being increasingly performed in the Asian Pacific area. This study systemically investigated the technique and clinical results for comparison with traditional radical subtotal gastrectomy for gastric cancers. Methods: Clinical studies evaluating the effectiveness and side effects of laparoscope-assisted gastrectomy in treating patients with gastric cancers were identified using a predefined search strategy. Summary rates of effectiveness and side effects of laparoscope-assisted gastrectomy were calculated. Results: Thirteen clinical studies which including 1,412 patients with gastric cancer treated by laparoscope-assisted gastrectomy were considered eligible for inclusion. Systemic analysis showed that, for all patients, the pooled resection rate was 100%. Major adverse effects were anastomotic stenosis, abdominal abscess, abdominal bleeding, postoperative ileus. Treatment related death occurred in 0. 71% (10/1412). Conclusion: This systemic analysis suggests that laparoscope-assisted gastrectomy in treating patients with gastric cancers is associated with good curative rate and acceptable complications.

Quantitative evaluation of through-thickness rectangular notch in metal plates based on lamb waves

Na Zhao,Bin Wu,Xiucheng Liu,Keqin Ding,Yanan Hu,Mahmoud Bayat 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.71 No.6

Lamb wave technology is a promising technology in the field of structural health monitoring and can be applied in the detection and monitoring of defects in plate structures. Based on the reconstruction algorithm for the probabilistic inspection of damage (RAPID), a Lamb-based detection and evaluation method of through-thickness rectangular notches in metal plates was proposed in this study. The influences of through-thickness rectangular notch length and the angle between sensing path and notch length direction on signals were further explored through simulations and experiments. Then a damage index calculation method which focuses on both phase and amplitude difference between detected signals and baseline signals was proposed. Based on the damage index difference between two vertically crossed sensing paths which pass through the notch in a sensor network, the notch direction identification method was proposed. In addition, the notch length was determined based on the damage index distribution along sensing paths. The experimental results showed that the image reconstructed with the proposed method could reflect the information for the evaluation of notches.

Clinical analysis and review of literature on pilomatrixoma in pediatric patients

Hu, Ju Long,Yoo, Hyokyung,Kwon, Sung Tack,Kim, Sukwha,Chung, Jee Hyeok,Kim, Hyeonwoo,Kim, Jinhyun,Yu, Na Hee,Kim, Byung Jun Korean Cleft Palate-Craniofacial Association 2020 Archives of Craniofacial Surgery Vol.21 No.5

Background: Pilomatrixoma is a benign tumor that originates from the hair follicle matrix. It usually presents as a hard, slow growing, solitary mass that can be easily misdiagnosed as other skin masses. The aim of this study was to clinically analyze a case series of pilomatrixoma in pediatric patients from Korea. Methods: A total of 165 pediatric patients from 2011 to 2018 with a histological diagnosis of pilomatrixoma were included. A retrospective review was performed using the electronic medical records, including patient demographics, number and location of the mass, clinical and imaging presentation, and postoperative outcomes. Results: There were 61 male and 104 female patients with 152 solitary and 13 multiple pilomatrixomas. Among solitary pilomatrixomas, the lesion commonly occurred in the head and neck (84.2%), followed by upper limbs (11.2%), lower limbs (3.3%), and trunk (1.3%). The pilomatrixoma lesion presented as the following types based on our clinical classification: mass (56.02%), pigmentation (25.31%), mixed (12.65%), ulceration (4.82%), and keloid-like (1.2%). Ultrasonography showed a high positive predictive value (95.56%). There were no specific complications observed except for two cases of recurrence. Conclusion: Pilomatrixoma has various clinical feature presentations and commonly occurs in the head and neck. Ultrasonography is a helpful diagnostic tool. Surgical removal of the lesion is the main treatment method with a low recurrence rate.

Prevention of radiation-induced tissue deformation by adiposederived stromal cells

Na Hee Yu,Julong Hu,Sung Tack Kwon,Byung Jun Kim 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.2

Antiviral Activities of Natural Compounds Derived from Traditional Chinese Medicines against Porcine Circovirus Type 2 (PCV2)

Na Sun,Tong Yu,Jun-Xing Zhao,Yaogui Sun,Junbing Jiang,Zhi-Bian Duan,WenkuiWang,Yuan-Liang Hu,Hai-Min Lei,HongquanLi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Twenty natural compounds isolated fromtraditional Chinese medicines were examined for theirantiviral activities against PCV2 in vitro. Antiviral activitywas analyzed by the assays of blocking of virus entry,inhibition of virus replication and inactivation of virusparticles in the virus suspension. 50% cytotoxic concentration(CC50) and 50% effective concentration (EC50) for eachcompound were determined by using the MTT method andimmunofluorescence assay (IFA), respectively. The resultsobtained from the inhibition of virus replication andinactivation of virus particles showed that the maximuminhibition rates for matrine and scutellarin were 57 and72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virusentry and inhibition of virus replication demonstrated thatboth aesculetin and arecoline hydrochloride stimulatedPCV2 replication, with the maximum stimulation rate of387.3 and 219.4%. In summary, both matrine and scutellarinhad antiviral activity against PCV2, while aesculetin andarecoline hydrochloride promoted PCV2 infection replicationin vitro.