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      • Fast, <i>In Situ</i> Demagnetization Method for Protection Current Transformers

        Zheng, Taiying,Hu, Ende,Yang, Huan,Zhao, Rongxiang,Kang, Yong Cheol,Wall, Peter,Terzija, Vladimir IEEE 2016 IEEE transactions on magnetics Vol.52 No.7

        <P>This paper proposes a fast, in situ demagnetization method for protection current transformers (CTs). Simulations show that the method can automatically demagnetize a CT within 300 ms in situ, i.e., while the CT is deenergized but still connected to the substation. Therefore, unlike existing methods that require the CT to be disconnected from the substation, this new method can be used to demagnetize a CT during a short interruption, e.g., the time it takes an autorecloser to operate, rather than requiring an extended outage of the substation. This will dramatically increase the number of opportunities for the demagnetization of protection CTs, which will alleviate the threat of remanent flux in the CT from causing saturation and compromising the performance of power system protection. Furthermore, by demagnetizing the CT in situ, the method avoids the risk of the improper reconnection of the CT, creating a source of hidden failure. The new method uses the existing dc voltage source in the substation to charge the CT and a parallel-connected inductor. An automatic control scheme is presented that manages the charge/discharge of the inductor, and thereby the demagnetization, by controlling three switches that reconfigure an RLC circuit. The additional hardware required by the method is the three switches, the switch controller, and the RLC circuit. The new method is tested for different hysteresis characteristics, levels of remanent flux, and errors in the assumed element parameters and measured hysteresis characteristics using EMTP-RV simulations. The results indicate that the proposed method can quickly and effectively demagnetize a protection CT in situ.</P>

      • KCI등재

        Improved Methodology for Identification of Cryptomonads: Combining Light Microscopy and PCR Amplification

        ( Shuang Xia ),( Ying Yin Cheng ),( Huan Zhu ),( Guo Xiang Liu ),( Zheng Yu Hu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3

        Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde, formaldehyde, and their combinations to preserve field samples collected from various waters, and the currently used fixative, Lugol`s solution was tested for comparison. Results showed that among the fixatives tested, glutaraldehyde preserved the samples best, and the optimal concentration of glutaraldehyde was 2%. The cell morphology was well preserved by glutaraldehyde. Cells kept their original color, volume, and shape, and important taxonomic features such as furrow/gullet complex, ejectosomes, as well as flagella could be observed clearly, whereas these organelles frequently disappeared in Lugol`s solution preserved samples. The osmotic adjustments and buffers tested could not preserve cell density significantly higher. Statistical calculation showed the cell density in the samples preserved by 2% glutaraldehyde remained stable after 43 days of the fixation procedure. In addition, DNA was extracted from glutaraldehyde preserved samples by grinding with liquid nitrogen and the 18S rDNA sequence was amplified by PCR. The sequence was virtually identical to the reference sequence, and phylogenetic analyses showed very close relationship between it and sequences from the same organism. To sum up, the present study demonstrated that 2% unbuffered glutaraldehyde, without osmotic adjustments, can preserve cryptomonads cells for identification, in terms of both light microscopy and phylogenetic analyses based on DNA sequences.

      • OPTIMIZATION DESIGN OF THE DIE OF SYNCHRONIZATION ROLLING AXLE ON CROSSWEDGEROLLING WITH MULTI-WEDGE

        Shu Xue dao,Li Chuan min,Hu Zheng huan 한국소성가공학회 2010 기타자료 Vol.2010 No.6

        For the rolling axle on Cross-Wedge Rolling with Multi-wedge, the key question is to guarantee the even distortion on each spot of the rolled pieces. In this paper, it has been taken that technological parameters of various wedges and the order of the rolling task completed by the third wedge as the design variable, the final rolled piece of the far even distortion as the research aim. By using method combined with the optimization design and the finite element method, the multi-wedge die of railway axle has been designed, in the meantime, its experimental verification has been given. The findings presents the rationale to realize the economization and the highly effective production on synchronization rolled axle of Cross-Wedge Rolling with Multi-wedge.

      • KCI등재

        A practical method for simultaneous determination of Poisson’s ratio and Young’s modulus of elasticity of thin films

        Jun-yi Sun,Jian-li Hu,Zhou-lian Zheng,Xiao-ting He,Huan-huan Geng 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.12

        In this paper, based on the exact analytical solution of axisymmetric deformation of the circular membrane fixed at its edge under the action of uniformly-distributed loads, we propose a new method to be able to simultaneously determine Poisson's ratio and Young's modulus of elasticity for thin films. We also present a set of exact formulas used for simultaneously determining Poisson's ratio and Young's modulus of elasticity for free-standing thin films or coating thin films without residual stresses or pre-tension. This method has the advantages of simplicity and reliability, and it doesn’t involve the substrate effect.

      • KCI등재

        Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host

        Zhongchen Ma,Shuifa Yu,Kejian Cheng,Yuhe Miao,Yimei Xu,Ruirui Hu,Wei Zheng,Jihai Yi,Huan Zhang,Ruirui Li,Zhiqiang Li,Yong Wang,Chuangfu Chen 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

        Background: Brucella infection induces brucellosis, a zoonotic disease. The intracellular circulation process and virulence of Brucella mainly depend on its type IV secretion system (T4SS) expressing secretory effectors. Secreted protein BspJ is a nucleomodulin of Brucella that invades the host cell nucleus. BspJ mediates host energy synthesis and apoptosis through interaction with proteins. However, the mechanism of BspJ as it affects the intracellular survival of Brucella remains to be clarified. Objectives: To verify the functions of nucleomodulin BspJ in Brucella's intracellular infection cycles. Methods: Constructed Brucella abortus BspJ gene deletion strain (B. abortus ΔBspJ) and complement strain (B. abortus pBspJ) and studied their roles in the proliferation of Brucella both in vivo and in vitro. Results: BspJ gene deletion reduced the survival and intracellular proliferation of Brucella at the replicating Brucella-containing vacuoles (rBCV) stage. Compared with the parent strain, the colonization ability of the bacteria in mice was significantly reduced, causing less inflammatory infiltration and pathological damage. We also found that the knockout of BspJ altered the secretion of cytokines (interleukin [IL]-6, IL-1β, IL-10, tumor necrosis factor-α, interferon-γ) in host cells and in mice to affect the intracellular survival of Brucella. Conclusions: BspJ is extremely important for the circulatory proliferation of Brucella in the host, and it may be involved in a previously unknown mechanism of Brucella's intracellular survival.

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