Galangin Prevents Acute Hepatorenal Toxicity in Novel Propacetamol-Induced Acetaminophen-Overdosed Mice

Ming-Shiun Tsai,Chia-Chih Chien,Ting-Hui Lin,Chia-Chi Liu,Rosa Huang Liu,Hong-Lin Su,Yung-Tsung Chiu,Sue-Hong Wang 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.11

Acetaminophen (APAP) overdose causes severe liver and kidney damage. APAP-induced liver injury (AILI) represents the most frequent cause of drug-induced liver failure. APAP is relatively insoluble and can only be taken orally; however, its prodrug, propacetamol, is water soluble and usually injected directly. In this study, we examined the timedependent effects of AILI after propacetamol injection in mice. After analyses of alanine aminotransferase and aspartate aminotransferase activities and liver histopathology, we demonstrated that a novel AILI mouse model can be established by single propacetamol injection. Furthermore, we compared the protective and therapeutic effects of galangin with a known liver protective extract, silymarin, and the only clinical agent for treating APAP toxicity, N-acetylcysteine (NAC), at the same dose in the model mice. We observed that galangin and silymarin were more effective than NAC for protecting against AILI. However, only NAC greatly improved both the survival time and rate consequent to a lethal dose of propacetamol. To decipher the hepatic protective mechanism(s) of galangin, galangin pretreatment significantly decreased the hepatic oxidative stress, increased hepatic glutathione level, and decreased hepatic microsomal CYP2E1 levels induced by propacetamol injection. In addition, propacetamol injection also reproduced the probability of APAP-induced kidney injury (AIKI), appearing similar to a clinical APAP overdose. Only galangin pretreatment showed the protective effect of AIKI. Thus, we have established a novel mouse model for AILI and AIKI using a single propacetamol injection. We also demonstrated that galangin provides significant protection against AILI and AIKI in this mouse model.

Transarterial Chemoembolization Using Gelatin Sponges or Microspheres Plus Lipiodol-Doxorubicin versus Doxorubicin-Loaded Beads for the Treatment of Hepatocellular Carcinoma

Yi-Sheng Liu,Ming-Ching Ou,Yi-Shan Tsai,Xi-Zhang Lin,Chien-Kuo Wang,Hong-Ming Tsai,Ming-Tsung Chuang 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.1

To retrospectively compare treatment of hepatocellular carcinoma (HCC) with transarterial chemoembolization (TACE) using gelatin sponges or microspheres plus lipiodol-doxorubicin vs. doxorubicin-loaded drug-eluting beads (DEB). A total of 158 patients with HCC received TACE from November 2010 to November 2011 were enrolled in this study, including 64 (40.5%) received TACE with lipiodol-doxorubicin and gelatin sponges (group A), 41 (25.9%) received TACE with lipiodol-doxorubicin and microspheres (group B), and 53 (33.5%) received TACE with doxorubicin-loaded DEB (group C). Tumor response and adverse events (AEs) were evaluated. No significant difference was found at baseline among the three groups. The doxorubicin dosage in group C wassignificantly (p < 0.001) higher compared to the dose used in groups A or B (median, 50 mg vs. 31 mg or 25 mg). Significantly (p < 0.001) more patients in group C achieved complete response compared to those in groups A or B (32.1% vs. 6.3% or 2.4%). Significantly (p < 0.001) less patients in group C had progressive disease compared to those in groups A or B (34.0% vs. 57.8% or 68.3%). Minor AEs were more common in groups A and B compared to group C, with rates of 54.7%, 34.1%, and 5.7%, respectively. In patients with HCC, TACE with DEB offers better safety and efficacy profiles compared to either TACE with gelatin sponges or TACE with microspheres.

DO BRAND IMAGE AND COUNTRY OF MANUFACTUR AFFECT CONSUMERS’ PERCEPTION OF LUXURY BRAND AFTER M&AS?

Hsiang-Ming Lee,Tsai Chen,Hong-Yew Lee 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.10

The purpose of this study is to examine the brand image and county of manufacture(COM) effect on brand luxury index, especially when the acquirer brand is afflicted by a low brand image and the acquired brand enjoys a high brand image. There were 248 responses in Taiwan (low image differences were gathered from 119 respondents and high image differences were gathered from 129 respondents). The results indicate that brand image have a positive influence on every dimension of brand luxury, while the better the luxury brand image acquired by one with an inferior image, the more the brand luxury will increase. Regarding the COM effect, the better COM image the acquirer move to, the more the brand luxury will increase. In addition, brand image and COM have interactive effect.

DO BRAND IMAGE AND COUNTRY OF MANUFACTUR AFFECT CONSUMERS’ PERCEPTION OF LUXURY BRAND AFTER M&AS?

Hsiang-Ming Lee,Tsai Chen,Hong-Yew Lee 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.7

The purpose of this study is to examine the brand image and county of manufacture(COM) effect on brand luxury index, especially when the acquirer brand is afflicted by a low brand image and the acquired brand enjoys a high brand image. There were 248 responses in Taiwan (low image differences were gathered from 119 respondents and high image differences were gathered from 129 respondents). The results indicate that brand image have a positive influence on every dimension of brand luxury, while the better the luxury brand image acquired by one with an inferior image, the more the brand luxury will increase. Regarding the COM effect, the better COM image the acquirer move to, the more the brand luxury will increase. In addition, brand image and COM have interactive effect.

Real-Time Target Tracking and Obstacle Avoidance for Mobile Robots using Two Cameras

Chan-Hong Chao,Bo-Yan Hsueh,Ming-Ying Hsiao,Shun-Hung Tsai,Tzuu-Hseng S. Li 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

This paper proposes an image processing approach for real-time target tracking and obstacle avoidance for mobile robot navigation in an indoor environment using two cameras. Several image processing techniques which include the averaging filter, edge-detection, erosion and dilation, and color segmentation are combined to find the target and obstacles. Then one can compute the angular position of the detected target and obstacle related to the mobile robot. The two cameras are utilized to calculate the relative distance of the target and obstacle from the mobile robot. According to the distance, one can determine the relationship of the target and obstacle to the mobile robot. The best target tracking path and obstacle avoidance path can be determined by different behavior modes. Therefore, the mobile robot plans a collision-free and successful track target to complete the patrol routine. Finally, the practical experiments demonstrate the feasibility and effectiveness of the proposed schemes.

Rapid screening of roundup ready soybean in food samples by a hand-held PCR device

Hsiang-Yun Tung,Sue-Hong Wang,Yu-Cheng Chiang,Ming-Shiun Tsai 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

Insulated isothermal PCR (iiPCR) method was recently available for rapid on-site detection ofroundup ready soybean (RRS; event GTS40-3-2) in food materials and products. Performance of thismethod was evaluated in this study. The 100% detection endpoint for the RRS by iiPCR was found insamples containing 0.1% RRS, equivalent to the results of the reference real-time PCR (rtPCR). Analysisof nucleic acids of soybean-based processed food products indicated 95% agreement between the iiPCRand rtPCR for RRS detection. By testing soybean milk and tofu samples using simple pretreatmentmethods, we found that the agreements between iiPCR and rtPCR methods of the aforementionedsamples were 80% and 90%, respectively. Replicated tests of all discrepant samples implied that thesesamples had trace amounts of RRS, suggesting that the iiPCR system is more sensitive than the rtPCRmethod. In conclusion, the iiPCR technology can be a useful point-of-need tool to help make a timelydecision in the consumption of genetically modified organisms.

Investigation on bragg reflection of surface water waves induced by a train of fixed floating pontoon breakwaters

Huei-Tau Ouyang,Kue-Hong Chen,Chi-Ming Tsai 대한조선학회 2015 International Journal of Naval Architecture and Oc Vol.7 No.6

The water wave characteristics of Bragg reflections from a train of fixed floating pontoon breakwaters was studied numerically. A numerical model of boundary discretization type was developed to calculate the wave field. The model was verified by comparing to analytical data in literature and good agreements were achieved. Series of parametric studies were conducted systematically to investigate the dependence of the reflected coefficients by the Bragg scattering on the design variables, including the spacing between the breakwaters, the total number of installed breakwaters, the draft and width do the breakwater, and wave length. Certain wave characteristics of the Bragg reflections were observed and discussed in details which might be of help for practical engineering applications in shoreline protection from incident waves.

Investigation on bragg reflection of surface water waves induced by a train of fixed floating pontoon breakwaters

Ouyang, Huei-Tau,Chen, Kue-Hong,Tsai, Chi-Ming The Society of Naval Architects of Korea 2015 International Journal of Naval Architecture and Oc Vol.7 No.6

The water wave characteristics of Bragg reflections from a train of fixed floating pontoon breakwaters was studied numerically. A numerical model of boundary discretization type was developed to calculate the wave field. The model was verified by comparing to analytical data in literature and good agreements were achieved. Series of parametric studies were conducted systematically to investigate the dependence of the reflected coefficients by the Bragg scattering on the design variables, including the spacing between the breakwaters, the total number of installed breakwaters, the draft and width do the breakwater, and wave length. Certain wave characteristics of the Bragg reflections were observed and discussed in details which might be of help for practical engineering applications in shoreline protection from incident waves.

Development of Novel Tool Center Point Velocity Planning Algorithm for Five Axis Machine Tool

Shih-Kai Wu,Meng-Shiun Tsai,Ming-Tzong Lin,Hong-Wei Huang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.8

In this paper, a tool center point (TCP) feedrate scheduling algorithm for 5-axis machine tool is developed to generate the axes interpolation commands. The proposed algorithm considers not only the constraints of TCP velocity, acceleration and jerk, but also the velocity differences of each axes at the junction of each block. First, the proposed method determines the maximum speed for each block segment at the TCP coordinate based on the computed length. Then the kinematics of the five axis machine tool is employed to derive the five-axis corner velocity difference (FCVD) formulation. The FCVD utilizes the axis velocity difference at the junction of blocks as the designed variable. As the starting and end velocities of each block are determined, the S-shape acceleration/ deceleration (Acc/Dec) method is adopted to generate both smooth TCP and rotary axis profile based on the given interpolation parameters. The servo dynamics of the five axis machine tool are utilized to evaluate the performances of the FCVD. Simulation results demonstrate that the FCVD can achieve better contour accuracy with less machining time as compared to the five-axis feedrate regulation formulation (FFRF) algorithm. Furthermore, the FCVD are compared with Heidenhain CNC controller and the results show that the FCVD has similar behaviors as the Heidenhain controller, but it can achieve less machining time.

Development of a 2-Chamber Culture System for Impedimetric Monitoring of Cell-cell Interaction

Kin Fong Lei,Meng-Tsan Tsai,Ming-Hong Zhong,Chia-Hao Huang,Ngan-Ming Tsang,Ming-Yih Lee 한국바이오칩학회 2017 BioChip Journal Vol.11 No.2

In cancer research, study of cell-cell interaction is important to understand tumor initiation, progression, metastasis, and therapeutic resistance. Conventionally, transwell system was adopted and cell proliferation was quantified by end-point bio-assays. The operations are labor-intensive and time-consuming while studying of the dynamic cellular responses of cell-cell interaction. Although impedance measurement was suggested to be a promising technique to monitor cellular responses, electrodes cannot be integrated into the transwell for the measurement purpose. In this work, a 2-chamber culture system incorporated with impedance measurement technique was developed to quantitatively study cell-cell interaction. The chamber was composed of 2 sub-chambers separated with a barrier. By this design, two types of cells could be independently cultured and concurrently monitored under common medium supplied. Cell-cell interaction was demonstrated by aberrant cell proliferation induced by the EGF secreted from the transfected cells cultured on another sub-chamber. Real-time and non-invasive monitoring of cell-cell interaction was successfully demonstrated. This work provides a practical solution for monitoring the dynamic cellular responses of cellcell interaction during the culture course. It is a reliable and convenient platform and facilitate more quantitative assessments in cancer research.