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Wideband 180° Phase Shifter Using Parallel-Coupled Three-Line
Yoon, Hong-Jib,Min, Byung-Wook THE INSTITUTE OF ELECTRICAL ENGINEERS 2019 Vol. No.
<P>In this letter, a wideband 180° phase shifter using a parallel-coupled three-line is demonstrated. The proposed phase shifter consists of a shorted coupled line and transmission line with shorted stubs. The bandwidth of the phase shifter is increased by a high coupling factor of the coupled line. When considering a coupling factor of 1, the bandwidth is infinite and the phase error is zero. Therefore, we adopted a parallel-coupled three-line to ensure a high coupling factor. Given a limited coupling factor, the transmission line impedance is optimized for the widest bandwidth of the return loss. The designed 180° phase shifter exhibits a return loss of less than −15 dB and a maximum phase error of less than 5° in the 1.14–2.79-GHz range and its fractional bandwidth is 83.9%.</P>
Two Section Wideband 90° Hybrid Coupler Using Parallel-Coupled Three-Line
Yoon, Hong-Jib,Min, Byung-Wook THE INSTITUTE OF ELECTRICAL ENGINEERS 2017 IEEE Microwave and Wireless Components Letters Vol. No.
<P>Two section wideband hybrid coupler with short-circuited coupled lines in the middle branch is demonstrated. The coupled lines are designed with a parallel-coupled 3-line, which has tight coupling and symmetric transmission phase over the center frequency. Without defected ground planes and a multilayer process, the proposed two-section coupler has the widest fractional bandwidth (FBW) than other two or even three section hybrid couplers modified from branch-line couplers. The designed wideband coupler has 55% FBW at the center frequency of 1.9 GHz. The bandwidth is limited by 1-dB power imbalance and the worst return loss, isolation, and phase imbalance within the bandwidth are 20.1 dB, 20.8 dB, and 3.2 degrees, respectively.</P>
보정 및 빔포밍 시간 최소화를 위한 묶음 REV와 최대값 보정 방법
윤홍집(Hong-jib Yoon),민병욱(Byung-Wook Min) 대한전자공학회 2017 대한전자공학회 학술대회 Vol.2017 No.6
In this paper, we proposed two new method. The fist method is bundle REV (Rotating electric-field vector), which significantly reduces the number of trials for array calibration. In the existing REV method, if array calibration is performed through N number of REV in the presence of N antennas, the proposed method can be reduced to logk N by combining k. The second method is the maximum value method, so that the total vector sum becomes the maximum when sweeping each element. In this way, we have confirmed that calibration and beam-forming times are minimized and that some accuracy is maintained.
Hong, Oak-Kee,Suh, Sun-Hee,Kwon, Hyuk-Sang,Ko, Seung-Hyun,Choi, Yoon-Hee,Moon, Sung-Dae,Yoo, Soon-Jib,Son, Ho-Young,Park, Kyung-Soo,Lee, In-Kyu,Yoon, Kun-Ho Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of cellular biochemistry Vol.95 No.4
<P>We have proposed that porcine neonatal pancreatic cell clusters (NPCCs) may be a useful alternative source of cells for islet transplantation, and that monolayer cultures might provide an opportunity to manipulate the cells before transplantation. In addition we previously identified 10 genes up-regulated by epidermal growth factor (EGF) in cultured porcine NPCC monolayers. We have now analyzed the intracellular signaling pathways activated by EGF and searched for proteins differentially expressed following EGF treatment of the monolayers, using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). EGF treatment resulted in phosphorylation of both Erk 1/2 and Akt, as well as increased cell proliferation. Five unknown and 13 previously identified proteins were differentially expressed in response to EGF. EGF treatment increased the expression of several structural proteins of epithelial cells, such as cytokeratin 19 and plakoglobin, whereas vimentin, the intermediate filament protein of mesenchymal cells, and non-muscle myosin alkali chain isoform 1, decreased. Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 factor, which promotes epithelial cell proliferation, and hemoglobin alpha I & II also increased, whereas cyclin A1, immunoglobulin heavy chain, apolipoprotein A1, 5,10-ethylenetetrahydrofolated reductase (5,10-MTHFR), angiotensin-converting enzyme 2 (ACE2), co-lipase II precursor, and NAD<SUP>+</SUP> isocitrate dehydrogenase (NAD<SUP>+</SUP> IDH) alpha chain proteins decreased. Our results show that EGF stimulates proliferation of pancreatic epithelial cells by simultaneously activating the MAPK and PI-3K pathways. HnRNP A2/B1, hemoglobin, cyclin A1, and ACE2 may play roles in the proliferation of epithelial cells in response to EGF. © 2005 Wiley-Liss, Inc.</P>
Yoon, Kun Ho,Yoo, Soon Jib,Kang, Moo-Il,Son, Hyun Shik,Hong, Kwan Soo CATHOLIC MEDICAL CENTER 1992 Bulletin of the Clinical Research Institute Vol.20 No.2
Genetic predispostition to the autoimmune disease insulin dependant diabetes mellitus is determined, in part, by a gene within the major histocompatibility complex (MHC) on human chromosome 6. After amplification of exon 2 HLA-DQα chain gene with polymerase chain reaction (PCR) procedure, we used non-radioactive HLA-DQα ASO probes which labelled with horseradish peroxidase (HRP) coupled to the 5’ end of the oligonucleotide for the HLA-DQα typing. The results were as follows: 1. The first domain elf the DQα chain gene was amplified from minimum 1 ng of genomic DNA with use of the PCR. And from 1 ㎍ DNA samples, all the samples were successfully amplified which have a product of approximately 242 bp. 2. On oligonulcleotide dot blot analysis of study subjects and standard control cell lines, TAB (HLA-DQAI homozygosity) cell line was hybridized to RH 83 probe only and DKB (HLA-DQA3 homozygosity) cell line to GH 67 probe and LUY (HLA-DQA4 homozygosity) cell line to GH 66. According to these results, we accurately determined HLA-DQα subtypes in study subjects. 3. Frequency of DQA3 allele in Korean was high and DQA2 allele was low compared to other populations. In IDDM patients, DQA3 allele w as significantly increased (relative risk 6.6, P=0.03) compared with controls. Conversely the frequencies of DQA1 allele was decreased among patients (relative risk 0.25, P=0.02). These results suggest that HLA-DQA3 allele is positively associated with IDDM as in other populations and HLA-DQA1 allele is negatively associated with IDDM in Korean population.
Transformation of Pisum sativum L. var sparkle; A Non Tissue Culture Method
CHOI, Hong Jib,PARK, Soon Ki,YOON, Young Hwi,KIM, Dal Ung 慶北大學校農業科學技術硏究所 1993 慶北大農學誌 Vol.11 No.-
완두에 있어서 보다 효율적인 형질전환 방법을 모색하고 형질전환된 개체를 얻고자 본실험을 수행하여 얻어진 결과는 다음과 같다. 형질전환은 발아중인 완두의 생장점(shoot tip)을 제거한 다음 T-DNA내에 GUS gene과 neomycin phosphotransferase Ⅱ gene이 들어있는 binary vector를 가진 Agrobacterium tumefaciens를 생장점을 제거한 부위에 감염시켰다. 감염후 새로 형성된 shoot는 개체당 4∼5개 였으며, 그중 GUS유전자가 발현하는 shoot만을 정상적인 식물체로 분화 시켰다. 감염부위에서 형성된 shoot에서의 GUS유전자의 발현빈도는 10%내외였다. 이들 개체로 부터 genomic DNA를 분리하여 Dot blot hybridization분석 결과 T-DNA가 식물체 내에 존재함을 알수 있었고, 수확한 종자를 발아시켜 Sorthern blot hybridization한 결과 T-DNA가 다음세대로 전달되었음이 확인되었으며 형질전환율은 2%이내였다. The transfer of genetic material into pea tissue was accomplished by using an avirulent strain of Agrobacterium tumefaciens containing the binary vector. The method used for transformation requires non-tissue culture steps as it involves the inoculation of the site of the shoot removed of germinating seeds. The identification of β-glucuronidase activity in the tissues of ?? pea plants indicates that the plant expressible β-glucuronidase gene, contained the T-DNA region from pLPBO2, had been transferred at least into somatic tissues. Putative transformed ?? pea plants were advanced to produce T₁plants which were also assayed for the presence of the transferred β-glucuronidase gene. The presence of the β-glucuronidase gene in DNAs isolated from T₁plant was demonstrated by DNA gel blot hybridization. This analysis revealed that the transformed plants contained β-glucuronidase gene.
Kim, Sang-Kuk,Choi, Hong-Jib,Kim, Kye-Ryung,Lee, In-Jung,Kim, Hak-Yoon The Korean Society of Crop Science 2011 한국작물학회지 Vol.56 No.3
The study was carried out to evaluate the affect of proton beam radiation on production of bulbil and tuber including change of endogenous gibberellins, of Dioscorea opposita Thunb. The yield of bulbils and tubers from non- and irradiated D. opposita Thunb at doses of 5, 10, 15 and 20 Gy were determined. Endogenous gibberellins were also quantified by GC/MS analysis. D. opposita tubers irradiated at 15 Gy produced higher bulbil production than non-irradiated plants. Enlarged bulbil (above size diameter 4 mm) was significantly increased at 15 Gy. Bioactive endogenous $GA_4$ was dominant in bulbils and tubers irradiated with proton beam rather than $GA_1$. Major gibberellins biosynthetic pathways in bulbils and tubers of D. opposita plants were non C-13 hydroxylation route. From the results of this study, 15 Gy proton beam radiation was suggested as an optimal dose that can produce high amounts of bulbil for mass production of D. opposita plant.