Korean Mistletoe Extract (KME) extends Life Span in Silk Worm Moth, Bombyx Mori

Hoe-Yune Jung,Jong-Bae Kim,Yung-Choon Yoo(유영춘) 한국실험동물학회 2009 한국실험동물학회 학술발표대회 논문집 Vol.2009 No.-

The Korean Mistletoe ( <i>Viscum album coloratum</i> ) Extract Has an Antiobesity Effect and Protects against Hepatic Steatosis in Mice with High-Fat Diet-Induced Obesity

Jung, Hoe-Yune,Kim, Yu-Hee,Kim, In-Bo,Jeong, Ju Seong,Lee, Jung-Han,Do, Myoung-Sool,Jung, Seung-Pil,Kim, Kwang-Soo,Kim, Kyong-Tai,Kim, Jong-Bae Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>This study investigates the inhibitory effects of Korean mistletoe extract (KME) on adipogenic factors in 3T3-L1 cells and obesity and nonalcoholic fatty liver disease (NAFLD) in mice fed a high-fat diet. Male C57Bl/6 mice fed a high-fat diet were treated with KME (3 g/kg/day) for 15 weeks for the antiobesity and NAFLD experiments. Body weight and daily food intake were measured regularly during the experimental period. The epididymal pad was measured and liver histology was observed. The effects of KME on thermogenesis and endurance capacity were measured. The effects of KME on adipogenic factors were examined in 3T3-L1 cells. Body and epididymal fat pad weights were reduced in KME-treated mice, and histological examination showed an amelioration of fatty liver in KME-treated mice, without an effect on food consumption. KME potently induces mitochondrial activity by activating thermogenesis and improving endurance capacity. KME also inhibited adipogenic factors <I>in vitro</I>. These results demonstrate the inhibitory effects of KME on obesity and NAFLD in mice fed a high-fat diet. The effects appear to be mediated through an enhanced mitochondrial activity. Therefore, KME may be an effective therapeutic candidate for treating obesity and fatty liver caused by a high-fat diet.</P>

Korean Mistletoe (Viscum album coloratum) Extract Improves Endurance Capacity in Mice by Stimulating Mitochondrial Activity

Hoe-Yune Jung,An-Na Lee,Tae-Jun Song,Hyo-Sun An,Young-Hoon Kim,Kyu-Dae Kim,In-Bo Kim,김경심,한백수,김천형,김광수,김종배 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.7

The beneficial effects of exercise on overall health make it desirable to identify the orally active agents that enhance the effects of exercise in an effort to cure metabolic diseases. Natural compounds such as resveratrol (RSV) are known to increase endurance by potentiating mitochondrial function. Korean mistletoe (Viscum album coloratum) extract (KME) has characteristics similar to those of RSV. In the present study, we determined whether KME could increase mitochondrial activity and exert an anti-fatigue effect. We found that KME treatment significantly increased the mitochondrial oxygen consumption rate (OCR) in L6 cells and increased the expression of peroxisome proliferator-activated receptor c coactivator (PGC)-1a and silent mating type information regulation 2 homolog 1 (SIRT1), two major regulators of mitochondria function, in C2C12 cells. In the treadmill test, KME-treated mice could run 2.5-times longer than chow-fed control mice. Additionally, plasma lactate levels of exhausted mice were significantly lower in the KME-treated group. In addition, the swimming time to exhaustion of mice treated with KME was prolonged by as much as 212% in the forced-swim test. Liver and kidney histology was similar between the KME-treated and phosphate-buffered saline-treated animals, indicating that KME was nontoxic. Taken together, our data show that KME induces mitochondrial activity, possibly by activating PGC-1a and SIRT1, and improves the endurance of mice, strongly suggesting that KME has great potential as a novel mitochondria-activating agent.

A <i> Fomitopsis pinicola Jeseng</i> Formulation Has an Antiobesity Effect and Protects against Hepatic Steatosis in Mice with High-Fat Diet-Induced Obesity

Jung, Hoe-Yune,Ji, Yosep,Kim, Na-Ri,Kim, Do-Young,Kim, Kyong-Tai,Choi, Bo-Hwa Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>This study investigated the antiobesity effect of an extract of the Fomitopsis pinicola Jeseng-containing formulation (FAVA), which is a combination of four natural components:<I> Fomitopsis pinicola Jeseng</I>;<I> Acanthopanax senticosus</I>;<I> Viscum album coloratum</I>; and<I> Allium tuberosum</I>. High-fat diet- (HFD-) fed male C57BL/6J mice were treated with FAVA (200 mg/kg/day) for 12 weeks to monitor the antiobesity effect and amelioration of nonalcoholic fatty liver diseases (NAFLD). Body and white adipose tissue (WAT) weights were reduced in FAVA-treated mice, and a histological examination showed an amelioration of fatty liver in FAVA-treated mice without decreasing food consumption. Additionally, FAVA reduced serum lipid profiles, leptin, and insulin levels compared with the HFD control group. The FAVA extract suppressed lipogenic mRNA expression levels from WAT concomitantly with the cholesterol biosynthesis level in the liver. These results demonstrate the inhibitory effects of FAVA on obesity and NAFLD in the diet-induced obese (DIO) mouse model. Therefore, FAVA may be an effective therapeutic candidate for treating obesity and fatty liver caused by a high-fat diet.</P>

한국산 겨우살이 추출물(KME)의 2형 당뇨 억제 및 근육세포 미토콘드리아 생성 증가 효과

정회윤(Hoe-Yune Jung),유영춘(Yung Choon Yoo),김인보(Inbo Kim),성낙윤(Nak Yun Sung),최옥병(Ok-Byung Choi),최보화(Bo-Hwa Choi),김종배(Jong-Bae Kim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.3

본 연구에서는 C57BL/6J ob/ob 마우스를 이용하여 한국산 겨우살이 냉수 추출물(KME)의 항당뇨 활성을 조사하였다. 50 혹은 100 mg/kg의 KME를 1일 1회씩 경구투여 한 결과 KME 투여 개시 5일 후부터 ob/ob 마우스의 혈당이 유의하게 억제되었으며, 10일 후부터 안정된 억제 효과를 나타내고 대조군에 비해 20% 이상의 혈당강하 효과를 나타내었다. 경구 당부하 실험(OGTT)에서는 KME 경구투여 마우스에서 유효한 당부하 억제 활성이 관찰되었다. 또한 KME 경구투여는 ob/ob 당뇨 마우스의 혈액 내 총 콜레스테롤과 중성지질의 농도를 억제하는 것으로 나타났다. 한편 C2C12 근육세포를 이용한 in vitro 실험에서 KME를 처리함으로써 glucose uptake가 현저히 증가하였다. 한편 매우 흥미롭게도 KME를 처리한 C2C12 근육세포에 있어서 미토콘드리아 생성과 산화대사 조절물질인 peroxisome proliferatoractivated receptor gamma coactivator 1-α(PGC-1α)를 비롯하여 glucose transporter type 4(GLUT4), estrogen-related receptor-α(ERR-α), nuclear respiratory factor-1(NRF-1) 그리고 mitochondrial transcription factor A(TmfA)와 같은 PGC-1α 관련 유전자들의 발현이 증가하는 것으로 확인되었다. 이 결과는 KME가 2형 당뇨에 대한 치료물질로서의 작용을 지니며 이러한 KME의 항당뇨 활성은 미토콘드리아 생성의 조절과 관련 있는 것으로 추정된다. In this study, the anti-diabetic activity of a cold water extract of Korean mistletoe (KME) was investigated in C57BL/6J Lep ob (ob/ob) mice. Oral administration of KME (50 or 100 mg/kg/d) significantly inhibited the level of blood glucose of ob/ob mice after 5 days from the beginning of KME treatment. And the anti-diabetic effect of KME was stabilized 10 days after oral administration, showing a substantial reduction of blood glucose levels by more than 20% as compared with control mice. The results of oral glucose tolerance test (OGTT) revealed that oral administration of KME gave rise to a remarkable improvement in overall glucose response. Oral administration of KME in ob/ob diabetic mice also significantly reduced blood total cholesterol (TCHO) and triglyceride (TG) levels compared with the diabetic control mice. Moreover, in an in vitro experiment using C2C12 myotubes, treatment of KME prominently increased glucose uptake. Interestingly, KME significantly increased the expression of peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α), a head regulator of mitochondrial biogenesis and oxidative metabolism, and PGC-1α-associated genes such as glucose transporter type 4 (GLUT4), estrogen-related receptor-α (ERR-α), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TmfA) in C2C12 cells. These results suggest that KME has potential as a novel therapeutic agent for diabetes, and its anti-diabetic activity may be related to the regulation of mitochondrial biogenesis.

Amodiaquine improves insulin resistance and lipid metabolism in diabetic model mice

Jung, Hoe‐,Yune,Kim, Bobae,Ryu, Hye Guk,Ji, Yosep,Park, Soyoung,Choi, Seung Hee,Lee, Dohyun,Lee, In‐,Kyu,Kim, Munki,Lee, You Jeong,Song, Woojin,Lee, Young Hee,Choi, Hyung Jin,Hyun, Chang&# Blackwell Publishing Ltd 2018 Diabetes, obesity & metabolism Vol.20 No.7

<P>Conclusion: Our findings suggest that amodiaquine exerts beneficial effects on glucose and lipid metabolism by concurrent activation of PPAR alpha/gamma. Furthermore, amodiaquine acts as an alternative insulin-sensitizing agent with a positive influence on lipid metabolism and has potential to prevent and treat type 2 diabetes while reducing the risk of lipid abnormalities.</P>

가시오가피를 이용한 기능성 건강음료의 제조

성미선(Mi-Sun Sung),정회윤(Hoe-Yune Jung),최준혁(Jun-Hyeok, Choi),이성철(Sung-Cheol Lee),최보화(Bo-Hwa Choi),박성선(Sung Sun Park) 한국생명과학회 2014 생명과학회지 Vol.24 No.9

본 연구에서는 가시오가피 줄기를 이용하여 농축액을 제조한 후 운동수행능력 증진 효과를 확인하고, 관능검사를 통해 가시오가피의 유용한 기능적인 특성을 살린 기호성 있는 건강 음료를 제조한 후 그 품질 특성을 조사하여 가공식품 및 식품소재로서 가시오가피의 활용도를 높이기 위한 기초 연구 자료로 제시하고자 하였다. 본 실험에서 사용한 건가시오가피 줄기의 일반성분 분석 결과 수분함량 6.50±0.12%, 조단백질 5.89±0.16%, 조지방 1.18±0.11%, 조회분 3.03±0.40%로 나타났다. 건가시오가피 줄기 추출물을 이용하여 제조한 가시오가피 농축액의 DPPH 라디칼 소거능은 증류수로 희석한 10% 농축액에서 87.42±1.63%로 나타나 positive control인 butylated hydroxy anisole (BHA) 1%의 DPPH 라디칼 소거활성 88.65±2.42%와 비슷한 수준으로 높은 활성을 보였으며, 최대운동수행능력을 측정한 결과 가시오가피 농축액을 섭취한 생쥐에서 대조군 생쥐에 비해 농도 의존적으로 운동수행 거리와 시간이 증가하는 것으로 확인되었다. 그리고 관능검사를 통해 선호도가 가장 높은 가시오가피 음료를 제조하여 그 특성을 확인한 결과 당도는 14.9 °Brix, pH는 4.51, 색도는 L값(명도) -62.70, a값(적색도) 3.67, b값(황색도) -6.12로 확인되었다. 따라서 본 연구의 결과는 가시오가피 농축액을 이용한 기호도가 증진된 기능성 음료로의 개발이 가능할 것으로 생각된다. This study was carried out to develop a functional healthy drink using 60% ethanol of dried Acanthopanax senticosus stem extract (ASE). The preparation, physical activity, anti-oxidant activity, and sensory properties of ASE were investigated. The moisture, crude protein, crude lipid, and ash contents of dried ASE were 6.50±0.12%, 5.89±0.16, 1.18±0.11, and 3.03±0.40, respectively. The 1,1-Diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity was 87.42±1.63% at 1/10 folds diluted ASE. In total, 40 male ICR mice were divided into five groups including the control (PBS), positive control (Red ginseng 200 mg/kg/day), and ASE-treated groups at doses of 35, 70, and 140 mg/kg/day for five weeks, respectively. ASE was administrated orally one time per day for five weeks before treadmill exercises, and normal and positive controls were fed PBS and red ginseng extract. In the treadmill test, ASE-treated mice (140 mg/kg/day) could run 1.4 times longer than the control mice. Healthy drinks were prepared with the addition of ASE at levels of 0.97% or 0.49% (A, B, and C type). Among the healthy drinks, the B type (ASE, 0.97%) was revealed to have the highest level of taste and overall acceptability through a sensory evaluation. The brix and pH of the ASE health drink (B type) were 14.9 and 4.51, respectively. These results indicated that the dried stem of Acanthopanax senticosus could be used as a functional material in the health drink industry.

Erratum: Affiliations Correction

정회윤,유영춘,김인보,성낙윤,최옥병,최보화,김종배,Jung, Hoe-Yune,Yoo, Yung Choon,Kim, Inbo,Sung, Nak Yun,Choi, Ok-Byung,Choi, Bo-Hwa,Kim, Jong-Bae The Korean Society of Food Science and Nutrition 2015 한국식품영양과학회지 Vol.44 No.6

Daphnane Diterpenes from <i>Daphne genkwa</i> Activate Nurr1 and Have a Neuroprotective Effect in an Animal Model of Parkinson’s Disease

Han, Baek-Soo,Kim, Kyoung-Shim,Kim, Yu Jin,Jung, Hoe-Yune,Kang, Young-Mi,Lee, Kyu-Suk,Sohn, Mi-Jin,Kim, Chun-Hyung,Kim, Kwang-Soo,Kim, Won-Gon American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.6

<P>Nurr1 is an orphan nuclear receptor that is essential for the differentiation and maintenance of dopaminergic neurons in the brain, and it is a therapeutic target for Parkinson's disease (PD). During the screening for Nurr1 activators from natural sources using cell-based assay systems, a methanol extract of the combined stems and roots of Daphne genkwa was found to activate the transcriptional function of Nurr1 at a concentration of 3 mu g/mL. The active components were isolated and identified as genkwanine N (1) and yuanhuacin (2). Both compounds 1 and 2 significantly enhanced the function of Nurr1 at 0.3 mu M. Nurr1-specific siRNA abolished the activity of 1 and 2, strongly suggesting that transcriptional activation by 1 and 2 occurred through the modulation of Nurr1 function. Additionally, treatment with 1 and 2 inhibited 6-hydroxydopamine (6-OHDA)-induced neuronal cell death and lipopolysaccharide (LPS)-induced neuroinflammation. Moreover, in a 6-OHDA-lesioned rat model of PD, intraperitoneal administration of 2 (0.5 mg/kg/day) for 2 weeks significantly improved behavioral deficits and reduced tyrosine hydroxylase (TH)-positive dopaminergic neuron death induced by 6-OHDA injection and had a beneficial effect on the inflammatory response in the brain. Accordingly, compounds 1 and 2, the first reported Nurr1 activators of natural origin, are potential lead compounds for the treatment of PD.</P>

여주(Momordica charantia) 추출물이 생쥐의 지구력 운동수행능력 향상 효과에 미치는 영향

김인보,박춘호,정회윤,정주성,홍환웅,김종배,Kim, Inbo,Park, Choon-Ho,Jung, Hoe-Yune,Jeong, Juseong,Hong, Hwan-Ung,Kim, Jong Bae 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.4

Bitter melon (Momordica charantia) is used in traditional herbal medicine in many Asian countries for the treatment of several diseases such as diabetes, eczema, night blindness, psoriasis, and rheumatism. Especially, most reports concerning the biological activities of bitter melon have focused on its effects on diabetes and hyperglycemia. Also, bitter melon is regarded as a longevity food, suggesting that it has several beneficial effects on anti-aging and the maintenance of a healthy state. Thus, we investigated whether bitter melon could increase the capacity of exercise in this study. Interestingly, bitter melon fruit extract activated AMP-activated protein kinase (AMPK), which is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. In addition, bitter melon extract increased the expression of enzymes involved in fatty acid oxidation such as mitochondrial uncoupling protein 3 (UCP3), carnitine palmitoyl transferase 1b (CPT1b), and pyruvate dehydrogenase lipoamide kinase isozyme 4 (PDK4). Moreover, exercise tolerance was much more enhanced in bitter melon treated animals compared to the non-treated control group. These results suggest that bitter melon is a promising candidate for the development of functional foods beneficial for physical strength and the enhancement of exercise capacity.