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Yang, Ha-Na,Lee, Seung-Eun,Jeong, Seong-Il,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong-Seek The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3
Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on $H_2O_2$-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.
Hana Yang,Seung Eun Lee,Seong Il Jeong,Cheung-Seog Park,Young-Ho Jin,Yong Seek Park 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3
Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on H<sub>2</sub>O<sub>2</sub>-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.
Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7
<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>
Ho-166 부착풍선도자를 이용한 방사선 조사의 돼지 관상동맥 스텐트 재협착 예방 효과
김원 ( Kim Won ),정명호 ( Jeong Myeong Ho ),박옥영 ( Park Og Yeong ),정우곤 ( Jeong U Gon ),박우석 ( Park U Seog ),김주한 ( Kim Ju Han ),안영근 ( An Yeong Geun ),조정관 ( Jo Jeong Gwan ),박종춘 ( Park Jong Chun ),강정채 ( Kang Je 한국지질동맥경화학회 ( 구 한국지질학회 ) 2002 韓國脂質學會誌 Vol.12 No.1
배경 : 국내에서 개발된 방사선 동위원소 Holmium-166 (166Ho)은 주로 베타선을 방출하며, 166Ho을 부착한 풍선도자를 이용하여 돼지 관상동맥 재협착 모형에서 풍선확장술 후 신생내막 증식을 전신적 부작용 없이 안전하고 효과적으로 억제하였음을 보고한 바 있다. 본 연구에서는 돼지 관상동맥 스텐트 재협착 모형에서 스텐트 시술 후 신생내막 증식에 의한 재협착 병변을 166Ho 부착 풍선도자를 이용하여 치료하여 그 효과를 관찰하고자 하였다. 방법
오석문(Oh Seog-Moon),홍순흠(Hong Soon-Heum),김성호(Kim Seong-Ho) 한국철도학회 2002 한국철도학회 학술발표대회논문집 Vol.- No.-
This paper is to investigate adoptability of the genetic algorithm to the railway traffic conflict resolution problem. We categorize the problem in two cases, one is the specific-boundary case and the other is not. GA is may be used in the first case, but not for second case. For a sample problem, an GA is designed, and the GA is being compared with Ho"s[2,3].
Chi, Jin Hua,Kim, Young Ho,Sohn, Dong Hwan,Seo, Geom Seog,Lee, Sung Hee Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.108 No.-
<P><B>Abstract</B></P> <P>Inflammatory bowel disease (IBD) is chronic inflammation of the gastrointestinal tract caused by high levels of pro-inflammatory cytokines and epithelial barrier dysfunction. <I>Alnus japonica</I> Steud. (Betulaceae) has been used in traditional Asian medicine. However, the potential of <I>A. japonica</I> for the treatment of intestinal inflammation has not been investigated. This study investigated the effects of ethanol extract from <I>A. japonica</I> bark (AJE) on colonic mucosa injury in mice with dextran sodium sulfate (DSS)-induced colitis. Treatment with AJE ameliorated pathological damage and the histopathologic features of DSS-induced colitis. The administration of AJE also inhibits DSS-induced pro-inflammatory cytokines expression, including interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and cyclooxygenase (COX)-2. Notably, AJE administration attenuated the reduction of tight junction proteins, zonula occludens (ZO)-1 and occludin, in DSS-induced colitis. In addition, AJE increased heme oxygenase (HO)-1 expression and prevented DSS-induced apoptosis in colonic epithelial cells. Furthermore, <I>in vitro</I> studies demonstrated that AJE inhibits TNF-α-induced IL-8, IL-1β, and COX-2 expression in human intestinal epithelial HT-29 cells and <I>tert</I>-butyl hydroperoxide-induced reduction of ZO-1 and occludin expression in human intestinal epithelial Caco-2 cells. AJE-induced HO-1 protein expression was also found in both HT-29 and Caco-2 cells. Taken together, our findings demonstrated that AJE inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis <I>in vivo</I> and human intestinal epithelial cells <I>in vitro</I>. These results suggest that AJE might have beneficial effects for the treatment of IBD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AJE attenuates the severity of DSS-induced colitis mice. </LI> <LI> AJE suppresses expression of pro-inflammatory mediators in DSS-induced colitis mice. </LI> <LI> AJE protects intestinal barrier integrity in DSS-induced colitis mice. </LI> <LI> AJE increases HO-1 expression in mouse colonic epithelial cells. </LI> <LI> AJE inhibits inflammation and protects loss of TJ proteins of human IEC cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism
Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2
Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.
변호준,이효진,김기석,천인국 순천향대학교 부설 산업기술연구소 2001 순천향 산업기술연구소논문집 Vol.7 No.2
To prevent from copying digital contents and to protect a copyright, it have used a watermark system which is a component of DRM(Digital Rights Management), When a watermark is extracted, a conventional watermark method needs original image. This needs additional cost, and it can't extract a watermark without original image.In this work, we propose a method to extract a watermark without original image. The proposed method uses a wavelet transformation to embed and extract a watermark. We experiment two cases for testing the proposed method, one thing is a extracting watermark using original watermark information, the other is a extracting watermark not using original watermarking information in extracting process. The proposed method, compared to conventional method which need a original image and a watermark image, offers a lot of convenience for managing digital contents.
고성호,한석영,최형연 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-
The median barrier is one of the roadside hardware to prevent severe human and property damage from highway traffic accidents. The foreign standard of concrete median barrier was introduced and implemented without modification fitting to domestic vehicle and highway condition. In a car accident, median barrier doesn't protect vehicle effectively, especially for heavy vehicle such as bus and heavy truck. The purpose of this study is to develop the optimal performance design of concrete median barrier using the design of experiment with crash simulation analysis which is done by Pam-Crash that is one of the commercial crash simulation software. As a result of this study, an optimal design of concrete median barrier is obtained considering von Mises stress, volume and COG acceleration of truck.