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Neurotropin protects rotator cuff tendon cells from lidocaineinduced cell death
Ryunosuke Abe,Hiroki Ohzono,Masafumi Gotoh,Yosuke Nakamura,Hirokazu Honda,Hidehiro Nakamura,Shinichiro Kume,Takahiro Okawa,Naoto Shiba 대한견주관절의학회 2021 대한견주관절의학회지 Vol.24 No.4
Background: Local anesthetics often are used in rotator cuff tears as therapeutic tools, although some cases have reported that they have detrimental effects. Neurotropin (NTP) is used widely in Japan as a treatment for various chronic pain conditions and is shown to have protective effects on cartilage and nerve cells. In this study, we investigated the protective effect of NTP against lidocaine-induced cytotoxicity. Methods: Tenocytes from rotator cuff tendons were incubated with lidocaine, NTP, lidocaine with NTP, and a control medium. Cell viability was evaluated using the WST-8 assay. Cell apoptosis was detected via annexin V staining using flow cytometry. The expression of BCL-2 and cytochrome c, which are involved in the intrinsic mitochondrial pathway of apoptosis, was evaluated via Western blotting and immunohistochemical staining. Results: In the cell viability assay, lidocaine decreased cell viability in a dose-dependent manner, and NTP did not affect cell viability. Moreover, NTP significantly inhibited the cytotoxic effect of lidocaine. The flow cytometry analysis showed that lidocaine significantly induced apoptosis in tenocytes, and NTP considerably inhibited this lidocaine-induced apoptosis. Western blotting experiments showed that lidocaine decreased the protein expression of BCL-2, and that NTP conserved the expression of BCL-2, even when used with lidocaine. Immunohistochemical staining for cytochrome c showed that 0.1% lidocaine increased cytochrome c-positive cells, and NTP suppressed lidocaine-induced cytochrome c expression. Conclusions: NTP suppresses lidocaine-induced apoptosis of tenocytes by inhibiting the mitochondrial apoptotic pathway. Intra-articular/bursal injection of NTP with lidocaine could protect tenocytes in rotator cuff tendons against lidocaine-induced apoptosis.
Inspiratory Muscle Training Effects on Respiratory Function and Coughing Ability in Men With Obesity
Yoshihiro Yamashina,Hiroki Aoyama,Tomoko Hirayama,Emiko Morita,Nami Sakagami,Hiroto Honda,Kazuyuki Tabira 물리치료재활과학회 2023 Physical therapy rehabilitation science Vol.12 No.4
Objective: Cough is a biological defense mechanism which produces sputum. Difficulties with expelling sputum can occur during decreased coughing ability caused by obesity, which increases the risk of pulmonary complications.We investigated the influence of an 8-week inspiratory muscle training (IMT) program on respiratory function and respiratory muscle strength in the sitting and supine positions of people with obesity. Design: A randomized controlled trial Methods: The participants were Twenty-one men with a body mass index (BMI) ≥ 30 kg/m2 or more. They were randomized into a control group (CG: n=10) and an IMT group (IMTG: n=11). The threshold IMT was used at a load of 30% of the maximum inspiratory oral pressure in the IMTG; the minimum load of the threshold IMT was used in the CG. Two sets of 15 min a day were performed four times a week for 8 weeks. Respiratory function, respiratory muscle strength, and coughing ability were measured in the sitting and supine positions. Results: Compared to baseline values, vital capacity in the supine position and inspiratory and expiratory muscle strength were significantly increased after 8 weeks in the IMTG, but not in theCG.Coughing ability did not differ significantly between the groups. Conclusion: Eight weeks of IMT enhanced respiratory muscle strength in men with obesity and improved vital capacity in the supine position;however, itdid not improve coughing ability.
( Shiro Nakamura ),( Hirotsugu Imaeda ),( Hiroki Nishikawa ),( Masaki Iimuro ),( Minoru Matsuura ),( Hideo Oka ),( Junsuke Oku ),( Takako Miyazaki ),( Hirohito Honda ),( Kenji Watanabe ),( Hiroshi Nak 대한장연구학회 2018 Intestinal Research Vol.16 No.4
Background/Aims: Noninvasive objective monitoring is advantageous for optimizing treatment strategies in patients inflammatory bowel disease (IBD). Fecal calprotectin (FCP) is superior to traditional biomarkers in terms of assessing the activity in patients with IBD. However, there are the differences among several FCP assays in the dynamics of FCP. In this prospective multicenter trial, we investigated the usefulness of FCP measurements in adult Japanese patients with IBD by reliable enzyme immunoassay using a monoclonal antibody. Methods: We assessed the relationship between FCP levels and disease or endoscopic activity in patients with ulcerative colitis (UC, n=64) or Crohn’s disease (CD, n=46) compared with healthy controls (HCs, n=64). Results: FCP levels in UC patients strongly correlated with the Disease Activity Index (r<sub>s</sub>=0.676, P<0.0001) and Mayo endoscopic subscore (MES; r<sub>s</sub>=0.677, P<0.0001). FCP levels were significantly higher even in patients with inactive UC or CD compared with HCs (P=0.0068, P<0.0001). The optimal cutoff value between MES 1 and 2 exhibited higher sensitivity (94.1%). FCP levels were significantly higher in active UC patients than in inactive patients (P<0.001), except those with proctitis. The Crohn’s Disease Activity Index tended to correlate with the FCP level (rs=0.283, P=0.0565). Conclusions: Our testing method using a monoclonal antibody for FCP was well-validated and differentiated IBD patients from HCs. FCP may be a useful biomarker for objective assessment of disease activity in adult Japanese IBD patients, especially those with UC. (Intest Res 2018;16:554-562)
Posterior decentering of the humeral head in patients with arthroscopic rotator cuff repair
Hidehiro Nakamura,Masafumi Gotoh,Hirokazu Honda,Yasuhiro Mitsui,Hiroki Ohzono,Naoto Shiba,Shinichiro Kume,Takahiro Okawa 대한견주관절학회 2022 대한견주관절의학회지 Vol.25 No.1
Background: In some patients with rotator cuff tear (RCT), the axial view of magnetic resonance imaging (MRI) shows subtle posterior decentering (PD) of the humeral head from the glenoid fossa. This is considered to result from a loss of centralization that is typically produced by rotator cuff function. There are few reports on PD in RCT despite the common occurrence of posterior subluxation in degenerative joint disease. In this study, we investigated the effect of PD in arthroscopic rotator cuff repair (ARCR). Methods: We conducted a retrospective study of consecutive patients who underwent ARCR at our institute and were followed-up for at least 1 year. PD was identified as a 2-mm posterior shift of the humeral head relative to the glenoid fossa in the axial MRI view preoperatively. The tear size and fatty degeneration (FD, Goutallier classification) were also evaluated using preoperative MRI. Retears were evaluated through MRI at 1 year postoperatively. Results: We included 135 shoulders in this study. Ten instances of PD (including seven retears) were observed preoperatively. Fifteen retears (three and 12 retears in the small/medium and large/massive tear groups, respectively) were observed postoperatively. PD was significantly correlated with tear size, FD, and retear occurrence (p<0.01 each). The odds ratio for PD in retears was 34.1, which was greater than that for tear size ≥3 cm and FD grade ≥3. Conclusions: We concluded that large tear size and FD contribute to the occurrence of PD. Furthermore, PD could be a predictor of retear after ARCR.
Yoshihisa Arao,Yuichi Sato,Satoru Hashimoto,Hiroki Honda,Kazumi Yoko,Masaaki Takamura,Ken-ichi Mizuno,Masaaki Kobayashi 대한소화기내시경학회 2015 Clinical Endoscopy Vol.48 No.6
A 50-year-old woman had a percutaneous endoscopic gastrostomy (PEG) tube placed after surgery for pharyngeal cancer. After 21 months, the PEG tube was removed due to improvement of per-oral ingestion. She had taken prednisolone for 31 years for systemic lupus erythematosus. The post-PEG fistula did not close spontaneously. The cause of the fistula was slow wound healing and gastrostomy site inflammation due to long-term steroid therapy. We were able to close the fistula with an over-the-scope clipping (OTSC) system. This case suggests that OTSC is useful for closing persistent post-PEG fistulas in patients receiving long-term prednisolone therapy.