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Controlled Lysis of Escherichia coli Double - Lysogen of Bacteriophages λHL1 and φ434
Koo, Yoon Mo,Lim, Henry C,Parekh, Bhavin S,Hatfield, G Wesley 한국화학공학회 1996 NICE Vol.14 No.3
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and, disruption. Construction of the doutrle-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. Thc single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Ø434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induce the double-lysogen to producx a protein and to lyse the host cell. The first phage gercome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cles, lacZ and defective Q genes was induced by increasing temperature to produce p-galactosidase, an intracellular reporter proteitc. The overproduction of 3-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second praphage from the lysogen MS21/Ø434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increasev of the extracxllular activity of galactosidase at the same time.
조무환,이진태,김준하,Henry C. Lim 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.3
A modified version of the IAWQ activated sludge model No. 1 (ASM 1) is adopted for the simulation of a sequencing batch reactor (SBR) to optimize the removal of nitrogen (T-N) and organic matters (COD) from wastewater. Since the removal of nitrogen requires both aerobic nitrification and anaerobic denitrification, we seek to find the optimal strategies of substrate fill and aeration. Substrate filling strategy critically influences the removal efficiency of T-N and COD; one fast discrete fill in the beginning of a cycle leads to the best result, while a slow continuous fill results in poor nitrification. In addition, the total aeration time is more important for the removal efficiency than the aeration frequency. A short aeration is beneficial for T-N removal, while a long aeration is beneficial for COD removal as expected. As a result, there is an optimal condition of aeration for the simultaneous removal of T-N and COD.
CONTROLLED LYSIS OF ESCHERICHIA COLI DOUBLE - LYSOGEN OF BACTERIOPHAGES λHL1 AND φ434
Koo, Yoon Mo,Parekh, Bhavin S,Hatfield, G Wesley,Lim, Henry C 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.2
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and disruption. Construction of the double-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. The single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Φ434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induice the double-lysogen to produce a protein and to lyse the host cell. The first phage genome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cI_(857), lacZ and defective Q genes was induced by increasing temperature to produce β-galactosidase, an intracellular reporter protein. The overproduction of β-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second prophage from the lysogen MS21/Φ434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increase of the extracellular activity of β-galactosidase at the same time.