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        Characterization of Ha29, a Specific Gene for Helicoverpa armigeraSingle-nucleocapsid Nucleopolyhedrovirus

        Chuan-Xi Zhang,Zhong-Jian Guo,Shi-Heng An,Dun Wang,Yan-He Liu,V. Shyam Kumar 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.3

        Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (O DVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

      • KCI등재

        Stochastic single machine scheduling with weighted quadratic early-tardy penalties

        Chuan-li Zhao,Heng-yong Tang 한국전산응용수학회 2008 Journal of applied mathematics & informatics Vol.26 No.5

        The problem of scheduling n jobs on a single machine is considered when the machine is subject to stochastic breakdowns. The objective is to minimize the weighted squared deviation of job completion times from a common due date. Two versions of the problem are addressed. In the first one the common due date is a given constant,whereas in the second one the common due date is a decision variable. In each case, a general form of deterministic equivalent of the stochastic scheduling problem is obtained when the counting process N(t) related to the machine uptimes is a Poisson process. It is proved that an optimal schedule must be V-shaped in terms of weighted processing time when the agreeable weight condition is satisfied. Based on the V-shape property, two dynamic programming algorithms are proposed to solve both versions of the problem.

      • KCI등재

        STOCHASTIC SINGLE MACHINE SCHEDULING WITH WEIGHTED QUADRATIC EARLY-TARDY PENALTIES

        Zhao, Chuan-Li,Tang, Heng-Yong Korean Society of Computational and Applied Mathem 2008 Journal of applied mathematics & informatics Vol.26 No.5

        The problem of scheduling n jobs on a single machine is considered when the machine is subject to stochastic breakdowns. The objective is to minimize the weighted squared deviation of job completion times from a common due date. Two versions of the problem are addressed. In the first one the common due date is a given constant, whereas in the second one the common due date is a decision variable. In each case, a general form of deterministic equivalent of the stochastic scheduling problem is obtained when the counting process N(t) related to the machine uptimes is a Poisson process. It is proved that an optimal schedule must be V-shaped in terms of weighted processing time when the agreeable weight condition is satisfied. Based on the V-shape property, two dynamic programming algorithms are proposed to solve both versions of the problem.

      • KCI등재

        Successful Enrichment of Rarely Found Candidatus Anammoxoglobus propionicus from Leachate Sludge

        ( Shu Chuan Hsu ),( Yen Chun Lai ),( Ping Heng Hsieh ),( Pun Jen Cheng ),( Suen Shin Wong ),( Chun Hsiung Hung ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7

        Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observation, denaturing gradient gel electrophoresis analysis, and cloning/sequencing techniques were applied to identify the diversity of anammox microorganisms throughout the incubation. During the early stage of operation, the diversity of anammox microorganisms was similar to the original complex microbes in the seed sludge. However, as incubation time increased, the anammox microorganism diversity within the system that was originally dominated by Candidatus (Ca.) Brocadia sp. was replaced by Ca. Anammoxoglobus propionicus. The domination of Ca. Anammoxoglobus propionicus produced a stable removal of ammonia (70 mg-N/l) and nitrite (90 mg-N/l), and the total nitrogen removal efficiency was maintained at nearly 95%. The fluorescence in situ hybridization results showed that Ca. Anammoxoglobus propionicus was successfully enriched from 1.8 ± 0.6% initially to 65 ± 5% after 481 days of operation. Therefore, the present results demonstrated the feasibility of enriching Ca. Anammoxoglobus propionicus from leachate sludge, even though the original cell count was extremely low. Application of this seldom found anammox organism could offer an alternative to current ammonia-nitrogen treatment.

      • KCI등재

        SINGLE-MACHINE SCHEDULING PROBLEMS WITH AN AGING EFFECT

        Zhao, Chuan-Li,Tang, Heng-Yong 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.25 No.1

        This paper considers single machine scheduling problems where the processing time of a job increases as a function of its position in the sequence. In this model, the later a given job is scheduled in the sequence, the longer its processing time. It is shown that the optimal schedule may be very different from that of the classical version of the problem. We introduce polynomial solutions for the makespan minimization problem, the sum of completion times minimization problem and the sum of earliness penalties minimization problem. For two resource constrained problems, based on the analysis of the problems, the optimal resource allocation methods are presented, respectively.

      • KCI등재

        Dynamic behavior analysis of push-the-bit rotary steerable bottom hole assembly

        Zhi-chuan Guan,Heng Wang,Yu-cai Shi,Wei-qing Chen,Guo-shan Zhao,Jian-yun Wang,Guang-qiang Cao 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.4

        Rotary steerable drilling technology is currently an advanced technology in the field of drilling engineering. A good understanding of dynamic behavior of rotary steerable system will benefit the drilling progress. Aimed at push-the-bit type of rotary steerable tool, finite element method was employed to model the rotary steerable bottom hole assembly (RSBHA) and establish the motion equation. Contact interaction between drill string and wellbore wall was analyzed and taken as the constraint condition in solving RSBHA motion equation. Force applied by steering pads was as the external force acting at the pads node. Then RSBHA motion state and bit lateral force were obtained by using Newmark method to solve the motion equation. A case was given and effects of influencing factors were analyzed. The results show that stick-slip phenomenon occurs under 60 r/min rotary speed condition and disappears when rotary speed rises to 90 r/min. Fully developed backward whirl comes into being at the near-bit stabilizer when rotary speed increases to 120 r/min. High weight on bit intensifies bit torsional vibration while larger pads’ steering force decreases the bit torsional vibration. Upper stabilizer plays a part in weakening RSBHA backward whirl under high rotary speed condition but reduces the inclination force. Time average of bit lateral force is mainly determined by pads’ steering force. Based on the results, corresponding suggestions were proposed to make the RSBHA be better used in the field applications.

      • KCI등재

        A Dynamic Hand Gesture Recognition System Incorporating Orientation-based Linear Extrapolation Predictor and Velocity-assisted Longest Common Subsequence Algorithm

        ( Min Yuan ),( Heng Yao ),( Chuan Qin ),( Ying Tian ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.9

        The present paper proposes a novel dynamic system for hand gesture recognition. The approach involved is comprised of three main steps: detection, tracking and recognition. First, the gesture contour captured by a 2D-camera is detected by combining the three-frame difference method and skin-color elliptic boundary model. Then, the trajectory of the hand gesture is extracted via a gesture-tracking algorithm based on an occlusion-direction oriented linear extrapolation predictor, where the gesture coordinate in next frame is predicted by the judgment of current occlusion direction. Finally, to overcome the interference of insignificant trajectory segments, the longest common subsequence (LCS) is employed with the aid of velocity information. Besides, to tackle the subgesture problem, i.e., some gestures may also be a part of others, the most probable gesture category is identified through comparison of the relative LCS length of each gesture, i.e., the proportion between the LCS length and the total length of each template, rather than the length of LCS for each gesture. The gesture dataset for system performance test contains digits ranged from 0 to 9, and experimental results demonstrate the robustness and effectiveness of the proposed approach.

      • KCI등재
      • KCI등재

        LSKL, a Peptide Antagonist of Thrombospondin-1, Attenuates Renal Interstitial Fibrosis in Rats with Unilateral Ureteral Obstruction

        Xi-sheng Xie,Fei-yan Li,Heng-chuan Liu,Yao Deng,Zi Li,Jun-ming Fan 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

        The effects of LSKL, the peptide antagonist of thrombospondin-1 (TSP-1), on renal interstitial fibrosis in rats subjected to unilateral ureteral obstruction (UUO) were investigated. Rats were divided randomly into three groups (n = 20 each): UUO group, sham-operation group and UUO plus LSKL treatment group. Collagen deposition was studied using histopathology and reverse transcription polymerase chain reaction analysis (RT-PCR). TSP-1, transforming growth factor beta 1 (TGF-β1), phosphorylated Smad2 (pSsmad2) and α-smooth muscle actin (α-SMA) in the kidney were measured using immunocytochemistry, western blotting analysis, RT-PCR and enzyme-linked immunosorbent assay. Biochemical analyses in the serum and urine were made. Histopathology showed severe tubular dilatation and atrophy, interstitial inflammation and collagen accumulation after surgery and LSKL significantly inhibited interstitial fibrosis including tubular injury as well as collagen deposition. The protein and mRNA levels of TSP-1 increased notably at different time point and significantly decreased in the presence of LSKL. The expression of TGF-β1 and pSmad2 were upregulated in the obstructed kidney and substantially suppressed by LSKL treatment. Myofibroblast accumulation could be alleviated after administration of LSKL. Biochemical parameters did not show differences among the three groups. As TSP-1 is the major activator of TGF-β1, we demonstrate that LSKL can attenuate renal interstitial fibrosis in vivo by preventing TSP-1-mediated TGF-β1 activation.

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