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보문 : 버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 분비 독소(tolaasin)를 저해하는 미생물 Pseudomonas sp. HC1
이찬중 ( Chan Jung Lee ),유영미 ( Young Mi Yoo ),한주연 ( Ju Yeon Han ),전창성 ( Chang Sung Jhune ),정종천 ( Jong Chun Cheong ),문지원 ( Ji Won Moon ),서장선 ( Jang Sun Suh ),한혜수 ( Hye Su Han1 ),차재순 ( Jae Soon Cha ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.4
A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA.. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.
Sang Ik Han(韓尙益),Hyun Tae Kim(金賢泰),Yil Chan Kwon(權一瓚),Chang Kie Park(朴昶璂),Yong Ho Kwack(郭龍鎬),Ill Kyung Chung(鄭鎰炅) 한국육종학회 1997 한국육종학회지 Vol.29 No.1
This study was conducted to determine the pattern of self-incompatibility through self-and cross-pollination in two varieties of herbaceous peony (Paeonia lactiflora Pallas). After self-pollination with the pollens of same variety, pollen tubes have germinated and grown into style tissue. However, the pollen tubes have arrested the growth in the mid of growing stage. This pattern was a different from that of sporophytic self-incompatibility. In the case of sporophytic self-incompatibility, self-pollinated pollens are not germinated on the style in normal, but if the pollens do, they do not penetrate into style tissue. From these phenomena, the pattern of self-incompatibility in herbaceous peony is assumed gametophytic self-incompatibility. For more precise research, protein analysis using SDS-PAGE and RNase activity stain gel electrophoresis was conducted. In SDS-PAGE analysis, two major proteins (21.5 and 28-kDa) was detected, and ribonuclease activity was tested in activity stain gel. Ribonucleatic activity is a unique characteristic of stylar proteins of gametophytic self-incompatibility plants reported previously. From these results, we concluded that P. lactiflora has a gametophytic self-incompatibility.
Han S. Uhm,Kyung H. Lee,Soon C. Cho,Sung Y. Jo,Chan U. Bang,Dong K. Lee 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
Surface treatments of Y2O3:Eu phosphors for improving the hydrophobic property were carried out using plasmas generated from a O-(Si(CH3)3)2 (HMDSO) in a low-pressure environment. The super-hydrophobic properties of the treated Y2O3:Eu phosphors were investigated. The samples treated by using the HMDSO/toluene glow plasma were analyzed by FTIR and OES and analyzed in terms of their contact angles (CAs). The effects of the HMDSO/toluene plasma treatments on the Y2O3:Eu phosphors were investigated in terms of the total surface free energy by using probe liquids to measure their contact angles (CAs). The total surface free energy of the Y2O3:Eu phosphors before and after a surface treatment with the HMDSO/toluene plasma, as estimated via the Owens-Wendt equation, was found to decrease from 82.2 mJ/m2 to 0.021 mJ/m2. This represents a significant improvement of the hydrophobicity of the Y2O3:Eu phosphors after the plasma treatment.
Poster Session : Separation and physiological activities of red pigment from Cordyceps pruinosa
( Chan Ho Lee ),( Deok Hyo Yoon ),( Ho Gyoung Kim ),( Sang Kuk Han ),( Ki Chul Hwang ),( Ki Sook Yang ),( Hae Il Park ),( Jae Mo Sung ),( Tae Woong Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-
Acute Toxicity Study of Recmvinant Human Interferon αA (LBD-007) in ICR Mice
Han, sang seop,Cha, Shin Woo,Song, Si Whan,Shin, Chan Young,Kim, Hyoung Chin,Shin, Chun Chul 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2
The acute toxicity of a recombinant human interferon αA (code name: LBD-007) was evaluated in both sexes of ICR mice, 5 weeks old, by the oral, subcutaneous and intravenous routes of administration. Based on the results, LBD-007 was not considered to induce any toxic effect on the mice in mortalities, clinical findigs, body weights and gross findings. It is suggested that LD_(50) values in mice would be >48x10^8 IU/kg in the oral, subcutaneous or intravenous routes.