cDNA Cloning and Expression Analysis of a Novel Human F-Box Only Protein

Haipeng Cheng,Yushu Ma,Xiaohua Ni,Min Jiang,Lingchen Guo,Wei Jin,Weiwen Xu,Gentao Cao,Chaoneng Ji,Kang Ying,Shaohua Gu,Yuhong Ma,Yi Xie,Yumun Mao 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1

F-box proteins are an expanding family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. During a large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone that encodes a novel F-box protein. It showed a 90.0% identity with the previously isolated mouse F-box protein16 at the amino acid level. Northern blot analysis showed no detectable expression, while re-verse transcription-polymerase chain reaction analysis indicated that FBXO16 was expressed in the heart, spleen, and colon. By mapping, we localized the FBXO16 gene to the human chromosome 8p12. The FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human genomic DNA.

Acoustic Monitoring in the Process of Pulsed Laser Paint Removal

Haipeng Huang,Bentian Hao,Dejun Ye,Yun Chen 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.7

Real-time monitoring of the laser paint removal process is critical to realize a good cleaning effect without causing damages to the base. Acoustic signals when the laser pulse continuously acts at one point were studied through an experiment. Results show that peak values and maximum frequency component of acoustic signals decreased to a stable value with paint removal, reflecting the cleaning process. According to an experimental study on cleaning acoustic signals during laser scanning, the cleaning process is divided into paint removal, clean finished, and excessive cleaning according to the damages threshold. The acoustic signals of the different processes were analyzed. Results show that variations of frequency components could reflect the paint removal process more accurately than time-domain signals. Extracting characteristic parameters of the maximum frequency component, spectrum, spectrum centroid amplitude, and spectrum centroid frequency from the frequency domain; introducing in the discriminant analysis; and establishing a Bayesian discriminant model according to cleaning characteristics can realize the quantitative discrimination of the paint removal process.

Deformation of the PDMS Membrane for a Liquid Lens Under Hydraulic Pressure

Haipeng Gu,Zihao Gan,Huajie Hong,Keyan He 한국광학회 2021 Current Optics and Photonics Vol.5 No.4

In the present study, a hyperelastic constitutive model is built by complying with a simplified hyperelastic strain energy function, which yields the numerical solution for a deformed polydimethylsiloxane (PDMS) membrane in the case of axisymmetric hydraulic pressure. Moreover, a nonlinear equilibrium model is deduced to accurately express the deformation of the membrane, laying a basis for precise analysis of the optical transfer function. Comparison to experimental and simulated data suggests that the model is capable of accurately characterizing the deformation behavior of the membrane. Furthermore, the stretch ratio derived from the model applies to the geometrical optimization of the deformed membrane.

A New Vehicle-Bridge Coupling Analysis Method Based on Model Polycondensation

Haipeng Shen,Xujun Chen,Kai Lu,Heng Huang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.1

Vehicle-bridge coupling analysis has always been a hot issue in bridge structure research. Model polycondensation technology is an effective calculation method for solving large-scale structural dynamic analysis. In this paper, three different model polycondensation theory was described, and based on the model polycondensation theory, a new vehicle-bridge coupling calculation idea is proposed, the corresponding calculation program is compiled by adopting MATLAB. In order to verify the theory and program, a vehicle-bridge model test was designed, and the 3-D numerical model of the vehicle and the bridge was established respectively. Modal analysis and the vehicle-bridge coupled transient analysis were carried out. The correctness of the calculation theory and program was verified by comparing the experimental values with the numerical simulation values, and the advantages and disadvantages of different methods are discussed by the comparation of the solving accuracy and time consumption.

Design and Implementation of an RFID Middleware Test Data Generator

Haipeng Zhang(장해붕),ChungKyu Park(박중규),Wooseok Ryu(류우석),Bonghee Hong(홍봉희) 한국정보과학회 2010 한국정보과학회 학술발표논문집 Vol.37 No.1A

Optimal Incremental-containment Control of Two-order Swarm System Based on Reinforcement Learning

Haipeng Chen,Wenxing Fu,Kang Chen,Junmin Liu,Dengxiu Yu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

In this paper, the optimal incremental-containment control of two-order swarm system based on reinforcement learning (RL) is proposed to avoid the dilemma that the number of agents in a swarm system is immutable, which is essential for a swarm system that cannot meet the containment demands and need more agents to expand the containment range. Notably, the number of agents in a swarm system with a traditional containment controller is immutable, which limits the containment range that the swarm system can achieve. Besides, in traditional optimal control theory, it is obtained by solving the Hamilton-Jacobi-Bellman (HJB) equation, which is difficult to solve due to the unknown nonlinearity. To overcome these problems, several contributions are made in this paper. Firstly, in order to overcome the dilemma that the number of agents in the swarm system is immutable, the incremental-containment control is proposed. Secondly, considering the error and control input as the optimization goal, the optimal cost function is introduced and the optimal incremental-containment control is proposed to reduce resource waste and increase hardware service life. Furthermore, based on the proposed optimal incrementalcontainment control, the controller is designed by a new RL based on the backstepping method. The Lyapunov function is used to prove the stability of controller. The simulation show the efficiency of the proposed controller.

Robust Fixed Time Control of a Class of Chaotic Systems with Bounded Uncertainties and Disturbances

Haipeng Su,Runzi Luo,Meichun Huang,Jiaojiao Fu 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.3

This paper investigates the fixed time control of a class of chaotic systems with uncertainties and disturbances via a novel fixed time theorem. By using variable substitution to calculate definite integral, a sufficient condition for fixed-time stability is firstly derived. Then, based on the given fixed time stable theory, a new nonlinear slide mode surface is proposed. By using a novel robust controller, the system’s state trajectories are driven to its origin in a fixed time which is independent on the initial value. The control strategy is applied to the permanent magnet synchronous motor (PMSM) chaotic system, and the results of numerical simulation demonstrate the effectiveness of the proposed schemes.

Significance of considering respiratory movement in estimating sleep stage

Haipeng Liu,Yuhang Xu,Dingchang Zheng 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.2

We read with great interest the paper “Sleep stage estimationmethods using a camera for home use” by Nochino et al. [ 1 ]. The paper proposed a method of sleep stage (wake, light,deep, and rapid-eye movement (REM)) estimation using anoff -the-shelf camera. Recognizing its potential in providingconvenient daily sleep monitoring for home use, we wouldlike to open a productive discussion concerning the methodologicalerrors and limitations presented in the paper.

Experimental and numerical research on cavitating flows around axisymmetric bodies

Wei Haipeng,Fu Song,Wu Qin,Huang Biao,Wang Guoyu 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.11

We investigated the cavitating flows around different axisymmetric bodies based on experiments and numerical simulation. In the numericalsimulation, the multiphase Reynolds averaged Navier Stokes equations (RANS) were solved via the commercial computationalfluid dynamics code CFX. The modified k-w SST turbulence model was used along with the transport equation-based cavitation model. In the experiments, a high-speed video technique was used to observe the unsteady cavitating flow patterns, and the dynamic force measurementsystem was used to measure the hydrodynamics of the axisymmetric bodies under different cavitation conditions. Results areshown for the hemisphere bodies, conical bodies and blunt bodies. Reasonable agreements were obtained between the computational andexperimental results. The results show that for the hemispherical body, the cavity consists of quasi-steady transparent region and unsteadyfoggy water-vapor mixture region, which contains small-scale vortices and is dominated by bubble clusters, causing irregulardisturbances at the cavity interfaces. The curvature at the front of the conical body is larger, resulting in that the flow separates at theshoulder of the axisymmetric body. The cavity stretches downstream and reaches to a fixed cavity length and shape. For blunt bodies, theincipient cavitation number is larger than that for the hemispherical body. A large cloud cavity is formed at the shoulder of the blunt bodyin the cores of vortices in high shear separation regions and the re-entrant jet does not significantly interact with the cavity interface whenit moves upstream. As to the dynamic characteristics of unsteady cavitating flows around the axisymmetric bodies, the pulsation frequencyfor the hemispherical body is larger than that for the blunt body. For the hemispherical body, the pulsation is mainly caused bythe high-frequency, small-scale shedding at the rear end of the cavity, while for the blunt body, the main factor for the pulsation frequencyis the periodically shedding of large-scale vortex cavities.

Peptidoglycan activation of the proPO-system without a peptidoglycan receptor protein (PGRP)?

Liu, Haipeng,Wu, Chenglin,Matsuda, Yasuyuki,Kawabata, Shun-ichiro,Lee, Bok Luel,Sö,derhä,ll, Kenneth,Sö,derhä,ll, Irene Elsevier 2011 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.35 No.1

<P><B>Abstract</B></P><P>Recognition of microbial polysaccharide by pattern recognition receptors triggers the prophenoloxidase (proPO) cascade, resulting in melanin synthesis and its deposition on the surface of invading pathogens. Several masquerade-like proteins and serine proteinase homologues have been shown to be involved in the proPO activation in insects. In this study, a novel serine proteinase homologue, <I>Pl</I>-SPH2, was found and isolated as a 30kDa protein from hemocytes of the freshwater crayfish, <I>Pacifastacus leniusculus</I>, by its binding property to a partially lysozyme digested or TCA-treated insoluble Lysine (Lys)-type peptidoglycan (PGN) and soluble polymeric Lys-type PGN. Two other proteins, the <I>Pl</I>-SPH1 and lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) were also found in the several different PGN-binding assays. However no PGRP homologue was detected. Neither was any putative PGRP found after searching available crustacean sequence databases. If RNA interference of <I>Pl</I>-SPH2, <I>Pl</I>-SPH1 or LGBP in the crayfish hematopoietic tissue cell culture was performed, it resulted in lower PO activity following activation of the proPO-system by soluble Lys-type PGN. Taken together, we report for the first time that Lys-type PGN is a trigger of proPO-system activation in a crustacean and that two <I>Pl</I>-SPHs are involved in this activation possibly by forming a complex with LGBP and without a PGRP.</P>