Compounds from the subterranean part of Johnsongrass and their allelopathic potential

Liu, Yan,Zhang, Chaoxian,Wei, Shouhui,Cui, Hailan,Huang, Hongjuan The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.3

The allelopathic effects of the exotic invasive weed, Johnsongrass, on Lactuca sativa, a native plant in China, were evaluated and the phytotoxins were investigated under laboratory conditions. The crude extracts (chloroform and ethyl acetate fractions) that were obtained from the ethanol extract of the subterranean parts of Johnsongrass inhibited the germination speed and growth of the roots and shoots of the test plant. The four compounds, ethyl p-hydroxybenzoate, diosmetin, apigenin, and luteolin, were isolated from the extract of the subterranean parts of Johnsongrass first, along with three other compounds (reported previously), p-hydroxybenzaldehyde, p-hydroxybenzoic acid, and dhurrin, and they all were evaluated on L. sativa. At the concentration of <0.5 mmol $L^{-1}$, ethyl p-hydroxybenzoate, dhurrin, and the mixture of the compounds delayed the germination speed of the seeds of L. sativa at 24 h of incubation, while apigenin delayed the germination speed of the seeds of L. sativa at 72 h of incubation. Ethyl p-hydroxybenzoate demonstrated the strongest delaying effect among the phenols and a similar effect was found with apigenin among the flavonoids, wheras ethyl p-hydroxybenzoate induced a drastic inhibition of the germination at 2 mmol $L^{-1}$. In contrast, p-hydroxybenzaldehyde and luteolin had no effect on the germination at any concentration at any stage. All the substances inhibited the shoot and root growth of L. sativa at 3 mmol $L^{-1}$. Increasing the concentration increased the inhibition of the growth of L. sativa.The inhibitory activity of ethyl p-hydroxybenzoate and p-hydroxybenzaldehyde was greater than that of the other compounds. This result suggested that the isolated phytotoxins might contribute to the successful invasion by Johnsongrass.

Predicting the Weed Control Efficacy of Photosynthesis Inhibitors by PPM Method

HONGJUN ZHANG,HAILAN CUI,JIMING YE,XUE LIU,XIANGJUN LI,CORNE KEMPENAAR,SHOUHUI WEI,HANWEN NI 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.3

The experiment was conducted in a greenhouse in Beijing, China, to determine whether weed control efficacy could be predicted based on Plant Photosynthesis Meter (PPM) values measured shortly after the application of photosynthesis inhibitors. The decrease of PPM values of the weed leaves treated with the mixture of atrazine and bentazone could be measured 1 day after treatment (DAT). The decreased extents depended on weed susceptibility and application dosage. There was significant correlation between the PPM values of the treated leaves 1 to 3 DAT and the biomass reduction 14 DAT. The experimental findings indicated that PPM values measured in early days after treatment could give reasonable prediction on weed control. The herbicide usually provided 90% control by weed biomass when PPM value was about 20 and relatively poor control when PPM value over 20.

Analysis of Antenna Selection in Two-way Relaying MIMO Systems with CPM Modulation

( Guowei Lei ),( Hailan Chen ),( Yuanan Liu ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.3

Up to now, many state-of-arts have been proposed for two-way relaying system with linear modulations. The performances of antenna selection (AS) at both transmit and relay nodes need to be investigated in some two-way relaying multiple-input multiple-output (TWRM) systems. In this paper, the goal is focused on the study of nonlinear modulations, i.e., continuous phase modulation (CPM) in TWRM systems. Firstly, the joint phase trellis are simplified by reversed Rimoldi processing so as to reduce the systems’ complexity. Then the performances of joint transmit and receive antenna selection (JTRAS) with CPM modulations in two-way relaying MIMO systems are analyzed. More exactly, the pair wise probability (PEP) is used to evaluate the error performance based on the CPM signal matrix, which is calculated in terms of Laurent expression. Since the channels subject to two terminal nodes share common antennas at relay node R in multiple-access scheme, we revise the JTRAS algorithm and compare it to existing algorithm via simulation. Finally, the error performances for various schemes of antenna selection are simulated and compared to the analysis in this paper.

Subproteomic profiling from renal cortices in OLETF rats reveals mutations of multiple novel genes in diabetic nephropathy

Li Zhiguo,Shen Hong,Liu Yeqiang,Zhou Xuefeng,Yan Meihua,He Hailan,Zhao Tingting,Zhang Haojun,Li Ping 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Ovarian cancer is a common gynecological malignancy among female patients and poses a serious threat to women's health. Although it has been established that Fos-like antigen 2 (FOSL2) is linked to ovarian cancer (OC), its exact role in the development of OC remains unknown. Objective: This article aims to investigate the role of FOSL2 in ovarian cancer development. Methods: FOSL2 expression in ovarian carcinoma and adjacent tissues was assessed using real-time fluorescent quantitative PCR and western blot. We constructed OE/sh-FOSL2 plasmids and Caspase-1 specific inhibitors (Yvad-CMK) and transfected A 2780 cells with them to identify the relevant cell functions. Furthermore, we used western blot assay to determine the changes in expression of apoptosis-associated speck-like protein containing a CARD (ASC), cysteine aspartate-specific proteasezymogen procaspase 1 (pro-caspase-1), cysteinyl aspartate-specific proteinase-1 (caspase-1), interleukin-1β precursor (pro-IL-1β), interleukin-1β (IL-1β), interleukin-18 precursor (pro-IL-18), and interleukin-18 (IL-18). In addition, we measured the concentration of IL-1β and IL-18 using an enzyme-linked immunosorbent assay (ELISA). Moreover, Tthe level of lactate dehydrogenase (LDH) in the cell supernatant was measured by LDH release assay kit. Results: The expression of FOSL2 was significantly higher compared with the surrounding tissues. The proliferation, migration, and invasion of A2780 cells were enhanced after transfection with OE-FOSL2 plasmids; however, the cell apoptosis was significantly decreased. When FOSL2 was overexpressed, the inflammasome-associated proteins such as ASC, caspase-1, IL-1β, and IL-18 were downregulated. Furthermore, FOSL2 induced apoptosis and activated the production of inflammasomes in A2780 cells. Co-therapy with Yvad-CMK and substantially inhibited apoptosis and activation of inflammasomes. Conclusions: Inhibition of FOSL2 promotes the apoptosis of OC cells by mediating the formation of an inflammasome.

Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

Li Fang,Sun Hailan,Ran Guangjun,Liu Xinhong,Yi Ruokun,Tan Fang,Zhao Xin,Liu Huazhi 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5

The aim of this study was to investigate the effect of Lactobacillus plantarum HFY09 on gastric injury induced by HCl/ ethanol in Kunming mice. The results showed that HFY09-H inhibited any increases in gastric juice volume, maintained the normal pH value of gastric acid, and reduced the damage caused to the gastric mucosa and gastric wall, the inhibition rate on the injury area reaches 63.70%. Compared with the negative control group, HFY09 increased the levels of serum somatostatin (SS) and vasoactive intestinal peptide (VIP), and also decreased the levels of substance P (SP), endothelin-1 (ET-1), interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). In addition, real time fluorescent quantitative PCR (Q-PCR) also confirmed that high-dose HFY09 ( 109 CFU/kg/ day) upregulated the mRNA expression of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS), and downregulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). At the same time, the results of the HFY09 treatment group were similar to those of the ranitidine treatment group. These results indicate that HFY09 can prevent gastric injury induced by HCl/ethanol in vivo. Therefore, HFY09 may play a potential role in the treatment of gastric diseases.

In situ and Ex situ Conservation of Erioborya Plants in China

Youli Hu,Shunquan Lin,Xianghui Yang,Hailan Zhang,Chengming Liu,Xiaolong He,Yehua He 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Genome-wide analysis of Gro/Tup1 family corepressors and their responses to hormones and abiotic stresses in maize

Hongyou Li,Kaifeng Huang,Hanmei Du,Hongling Wang,Xin Chen,Shibin Gao,Hailan Liu,Moju Cao,Yanli Lu,Tingzhao Rong,Su-Zhi Zhang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.6

Gro/Tup1 proteins act as negative transcriptional regulators and play crucial roles in many growth and developmental processes in a wide range of organisms. However, our understanding of Gro/Tup1 protein functions in plants is confined to the model plant Arabidopsis. Here, 11 Gro/Tup1 genes, which were characterized by the typical LisH and WD40 repeat domains, were identified in maize through a genome-wide survey. A phylogenetic analysis revealed that maize Gro/Tup1 proteins could be divided into three subfamilies, in which members shared similar protein and gene structures. The predicted maize Gro/Tup1 genes were distributed on seven chromosomes and segmental duplication contributed to their expansion. Many predicted cis-elements associated with hormones, biotic- or abioticstress responses, meristem and seed development, and circadian rhythms, were found in their putative promoter regions. A potential associated protein analysis identified a large number of candidates, including transcription factors, chromatin-modifying enzymes, protein kinases, and ubiquitinconjugating enzymes. An expression profile derived from the RNA-seq data indicated that Gro/Tup1 genes in maize were widely expressed in various organs and tissues. Quantitative real-time PCR revealed that these genes responded to at least one hormone or abiotic stress, either in roots or in shoots. Our study provides useful information on the Gro/Tup1 genes in maize and will facilitate the further functional validation of these genes in growth and development, hormone responses, and biotic- or abiotic-stress resistance.