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리그닌 함유 셀룰로오스 나노섬유로 강화된 폴리락틴산 나노복합재의 제조 및 분석
Hai Bo Sun,Xuan Wang,Li Ping Zhang 한국고분자학회 2014 폴리머 Vol.38 No.4
A chemo-mechanical method was used to prepare lignin-containing cellulose nanofibrils(L-CNF) from unbleached woodpulps dispersed uniformly in an organic solvent. L-CNF/PLA composites were obtained by solvent cast-ing method. The effects of L-CNF concentration on the composite performances were characterized by tensile test machine. contact angle machine, scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). The tensile test results indicated that the tensile strength and elongation-at-break increased by 50.6%and 31.8% compared with pure PLA. The contact angle of PLA composites decreased from 79.3° to 68.9°. The FTIR analysis suc- cessfully showed that L-CNF had formed intermolecular hydrogen bonding with PLA matrix.
The Incidence, Changes and Treatments of Cervical Deformity After Infection and Inflammation
Bo Han,Jianqiang Wang,Yong Hai,Duan Sun,Weishi Liang,Peng Yin,Hongtao Ding 대한척추신경외과학회 2023 Neurospine Vol.20 No.1
A healthy cervical spine with normal movement is the basis of many daily activities and is essential for maintaining a good quality of life. However, the alignment, fusion, and structure of the cervical spine can change for various reasons, leading to cervical deformity, mainly kyphosis. Approximately 5%‒20% of spinal infections in the cervical spine cause cervical deformity. The deformity can recover early; however, the disease's long-term existence or the continuous action of abnormal stress may lead to intervertebral fusion and abnormal osteophytes. Many gaps and controversies exist regarding infectious cervical deformities, including a lack of clear definitions and an acceptable classification system thereby requiring further research. Moreover, there is no consensus on the indications for postinfectious cervical deformity associated with Mycobacterium tuberculosis, Staphylococcus aureus, and Brucellosis. Therefore, we reviewed and discussed the incidence, clinical manifestations, changes, and treatment of infectious and inflammatory secondary cervical deformities from common to rare to provide a theoretical basis for clinical decision-making.
Sun, Jian,Zhang, Shi-Jie,Li, Hai-Bo,Zhou, Wei,Hu, Wei-Xiao,Shan, Shang Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.5
Twenty two new 5-fluorouracil (5-FU) derivatives, 2-butoxy-4-substituted 5-fluoropyrimidines, were synthesized and characterized by IR, $^1H$ NMR, MS, HRMS. All compounds were preliminarily evaluated by MTT assay on human liver BEL-7402 cancer cell line in vitro. Ten compounds were selected to test their cytotoxic activity against A549, HL-60 and MCF-7 cancer cell lines in vitro. These compounds were more sensitive to BEL-7402 than other cell lines, particularly, cytotoxic activity of compounds 6b, 6d-f, 6p, 6s-u were in sub-micromolar scale. The highest cytotoxic potency against A549, HL-60 and MCF-7 was shown by 2-butoxy-4-chloro-5-fluoropyrimidine (5) with $IC_{50}$ values of 0.10, 1.66 and $0.59{\mu}M$, respectively. Compounds 6d and 6e were effective against MCF-7 with $IC_{50}$ $9.73{\mu}M$ and HL-60 with $IC_{50}$ $8.83{\mu}M$, respectively.
Jian Sun,Shi-Jie Zhang,Hai-Bo Li,Wei Zhou,Wei-Xiao Hu,Shang Shan 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.5
Twenty two new 5-fluorouracil (5-FU) derivatives, 2-butoxy-4-substituted 5-fluoropyrimidines, were synthesized and characterized by IR, 1H NMR, MS, HRMS. All compounds were preliminarily evaluated by MTT assay on human liver BEL-7402 cancer cell line in vitro. Ten compounds were selected to test their cytotoxic activity against A549, HL-60 and MCF-7 cancer cell lines in vitro. These compounds were more sensitive to BEL-7402 than other cell lines, particularly, cytotoxic activity of compounds 6b, 6d-f, 6p, 6s-u were in sub-micromolar scale. The highest cytotoxic potency against A549, HL-60 and MCF-7 was shown by 2-butoxy-4-chloro-5- fluoropyrimidine (5) with IC50 values of 0.10, 1.66 and 0.59 μM, respectively. Compounds 6d and 6e were effective against MCF-7 with IC50 9.73 μM and HL-60 with IC50 8.83 μM, respectively.
Liu, Hai-Bo,Kim, Jung Sun,Park, Sunghoon Mary Ann Liebert, Inc 2011 Journal of biomolecular screening Vol.16 No.9
<P>Quorum sensing (QS) is a cell density-dependent signaling system that is used by bacteria to coordinate gene expression within their population. In this study, the authors describe the development and characterization of various cell-based bioassay systems for detecting QS inhibitors based on three LuxR family proteins, TraR, LasR, and the recently identified QscR. Three different gram-negative bacteria, Escherichia coli, Agrobacterium tumefaciens, and Pseudomonas aeruginosa, were employed as reporter strains to overproduce one of the aforementioned QS activator proteins and respond to inhibitors. The nine different whole-cell assay systems (three reporter strains three QS proteins) were evaluated for their applicability and reliability by studying quantitative responses to various furanones, which are potent inhibitors of the LuxR family proteins. These results demonstrate that the cell-based bioassay systems are sensitive and reliable tools for screening of QS activators and inhibitors. This study also suggests that furanones are potentially important QS inhibitors for many LuxR-type activator proteins.</P>
Inhibitors of the Pseudomonas aeruginosa quorum-sensing regulator, QscR
Liu, Hai-Bo,Lee, Joon-Hee,Kim, Jung Sun,Park, Sunghoon Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and bioengineering Vol.106 No.1
<P>QscR is a quorum-sensing (QS) signal receptor that controls expression of virulence genes in the prevalent opportunistic pathogen, Pseudomonas aeruginosa. Unlike the previously reported LuxR-type QS receptor proteins, that is, LasR and TraR, QscR can be obtained as an apo-protein that can reversibly form an active complex in vitro with its cognate signal molecule, 3-oxododecanoyl-homoserine lactone (3OC12-HSL), and subsequently bind to target promoter DNA sequences. To search for potential QS inhibitors, an in vitro gel retardation assay was developed using the purified QscR. Both the in vitro assay and the in vivo cell-based assay using QscR-overproducing recombinant strains were applied in the screening process. Furanones were chosen for testing the activity as QS inhibitors because they have been reported to strongly inhibit expression of QS-related genes in Agrobacterium tumefaciens. Among more than a hundred furanones tested, three compounds showed strong and dose-dependent inhibitory effects on QscR in both assays. One compound in particular, designated as F2, could completely inhibit the 3OC12-HSL-dependent QscR activity in vitro at a concentration of 50-fold molar excess over 3OC12-HSL. However, with the furanones F3 and F4, which are structurally similar to F2 but with a nitro group instead of the amine moiety, significantly decreased activities were observed. These results suggest that (i) the in vitro assay is a sensitive and reliable tool for screening QS inhibitors, and (ii) furanones are potentially important QS inhibitors for many LuxR-type receptor proteins. Biotechnol. Bioeng. 2010; 106: 119–126. © 2010 Wiley Periodicals, Inc.</P>