http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Hafeza Akter (검색결과 10 건)
- 무료
- 기관 내 무료
- 유료
-
Validation of Neurotensin Receptor 1 as a Therapeutic Target for Gastric Cancer
Hafeza Akter,윤정환,유영숙,강민정 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.6
Gastric cancer is the fifth most common type of malignancy worldwide, and the survival rate of patients with advanced-stage gastric cancer is low, even after receiving chemotherapy. Here, we validated neurotensin receptor 1 (NTSR1) as a potential therapeutic target in gastric cancer. We compared NTSR1 expression levels in sixty different gastric cancer-tissue samples and cells, as well as in other cancer cells (lung, breast, pancreatic, and colon), by assessing NTSR1 expression via semi-quantitative real-time reverse transcription polymerase chain reaction, immunocytochemistry and western blot. Following neurotensin (NT) treatment, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) and further determined the effects on cell migra-tion and invasion via wound-healing and transwell assays. Our results revealed that NTSR1 mRNA levels were higher in gastric cancer tissues than non-cancerous tissues. Both of NTSR1 mRNA levels and expression were higher in gastric cancer cell lines relative to levels observed in other cancer-cell lines. Moreover, NT treatment induced MMP-9 expression and activity in all cancer cell lines, which was significantly decreased following treatment with the NTSR1 antagonist SR48692 or small-interfering RNA targeting NTSR1. Furthermore, NT-mediated metastases was confirmed by observing epithelial-mesenchymal transition markers SNAIL and E-cadherin in gastric cancer cells. NT-mediated invasion and migration of gastric cancer cells were reduced by NTSR1 depletion through the Erk signaling. These findings strongly suggested that NTR1 constitutes a potential therapeutic target for the inhibition of gastric cancer inva-sion and metastasis.
-
Validation of Neurotensin Receptor 1 as a Therapeutic Target for Gastric Cancer
Akter, Hafeza,Yoon, Jung Hwan,Yoo, Young Sook,Kang, Min-Jung Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6
Gastric cancer is the fifth most common type of malignancy worldwide, and the survival rate of patients with advanced-stage gastric cancer is low, even after receiving chemotherapy. Here, we validated neurotensin receptor 1 (NTSR1) as a potential therapeutic target in gastric cancer. We compared NTSR1 expression levels in sixty different gastric cancer-tissue samples and cells, as well as in other cancer cells (lung, breast, pancreatic, and colon), by assessing NTSR1 expression via semi-quantitative real-time reverse transcription polymerase chain reaction, immunocytochemistry and western blot. Following neurotensin (NT) treatment, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) and further determined the effects on cell migration and invasion via wound-healing and transwell assays. Our results revealed that NTSR1 mRNA levels were higher in gastric cancer tissues than non-cancerous tissues. Both of NTSR1 mRNA levels and expression were higher in gastric cancer cell lines relative to levels observed in other cancer-cell lines. Moreover, NT treatment induced MMP-9 expression and activity in all cancer cell lines, which was significantly decreased following treatment with the NTSR1 antagonist SR48692 or small-interfering RNA targeting NTSR1. Furthermore, NT-mediated metastases was confirmed by observing epithelial-mesenchymal transition markers SNAIL and E-cadherin in gastric cancer cells. NT-mediated invasion and migration of gastric cancer cells were reduced by NTSR1 depletion through the Erk signaling. These findings strongly suggested that NTR1 constitutes a potential therapeutic target for the inhibition of gastric cancer invasion and metastasis.
-
Akter, Hafeza,Park, Min,Kwon, Oh-Seung,Song, Eun Joo,Park, Won-Sang,Kang, Min-Jung Saikon Pub. Co 2015 TUMOR BIOLOGY Vol.36 No.8
<P>Neurotensin (NT) is distributed throughout the brain and gastrointestinal tract. Although the relationship between NT and matrix metalloproteinase-9 (MMP-9) activity in gastric cancer has not been reported, the elevation of MMP-9 and NT is reported in the breast, lung, prostate, and gastric cancer. The aim of our study is to investigate the relationship between NT and MMP-9 activity and the underlying signaling mechanism in gastric cancer cell lines. Commercial ELISA kits were used for estimation of NT and MMP-9 expression, and fluorescence resonance energy transfer (FRET) assay was used for measurement of MMP-9 activity. Cell migration and invasion were determined by wound healing and transwell assay. The expression of signaling proteins was measured by Western blotting. Our study reveals a positive correlation between increased plasma NT and MMP-9 activity in both of patient's serum and gastric cancer cell lines. A dose-dependent elevation of MMP-9 activity was observed by NT treatment in gastric cancer cells (MKN-1 and MKN-45) compared to untreated gastric cancer and normal epithelial cell (HFE-145). Moreover, NT-mediated migration and invasion were observed in gastric cancer cells unlike in normal cell. The signaling mechanism of NT in gastric cancer cells was confirmed in protein kinase C (PKC), extracellular-signal regulated kinase (ERK), and phosphatidylinositol 3-kinase (PI3K) pathway. In addition, pretreatment of gastric cancer cells with NTR1 inhibitor SR48692 was shown to significantly inhibit the NT-mediated MMP-9 activity, cell invasion, and migration. Our finding illustrated NTR1 could be a possible therapeutic target for gastric cancer.</P>
-
용액 안에 담겨 있는 항체와 결합용 자성나노입자의 자기저항 특성
이상석,최종구,Mahbub Hasan,Hafeza Akter,이현숙 한국자기학회 2022 韓國磁氣學會誌 Vol.32 No.3
Magnetic nanoparticles with an average diameter of less than 50 nm with an amine-group surface coated with silica or dextran are being used as immunotherapeutic antibody conjugates. The magnetoresistance (MR) characteristics of magnetic nanoparticles (MNPs) are investigated according to the strength of the external magnetic field that changed the distribution of the nanoparticle solution at a 1 mm interval between two copper electrodes having a diameter of 1 mm. In the case of the silica amine group MNP solution, which is rapidly separated by an external magnetic field, the magnetoresistance curve is non-uniform. In the case of MNPs with an antibody attached to an amine group, the initial MR value, MR ratio, and solution coercivity are 0.74M, 1.6%, and 200 Oe, respectively, depending on the external magnetic field. In addition, it took more than 10 hours under a powerful magnetic field to completely separate the magnetic dextran nanoparticles from which the dispersant is sufficiently added to a MNP solution of 2 mg/mL. The dextran MNP solution, which maintained the concentration, shows a symmetric butterfly-shaped magnetoresistance curve with MR, MR ratio, and solution coercive force of 1.43M, 0.2%, and 260 Oe, respectively, depending on the external magnetic field. These results show that it is possible to control the induction of MNPs for the development of antibodies for immunotherapy to a target with a magnetic field.
-
Mahbub Hasan,Jong-Gu Choi,Hafeza Akter,Sang-Suk Lee 한국자기학회 2022 Journal of Magnetics Vol.27 No.4
The suppression of cytokine storm in severe coronavirus disease 2019 (COVID-19) patients can be treated with monoclonal antibody therapy against CD3 for T cell receptor inhibition. An optimized liquid phase as a CD3 antibody-magnetic nanoparticle (Ab-MNP) conjugate can inhibit the overactivation of T cells. We aim to analyze the distribution of Fe in the spleen after acute administration of silica-conjugated amine magnetite (Fe3O4) nanoparticles (35 nm) delivered by intravenous injection. The Fe element distribution and concentration levels in spleen tissue were analyzed using energy dispersive spectroscopy (EDS) and inductively coupled plasmamass spectrometry (ICP-MS). The experimental result is a difference in the concentration of Fe elements, which was 1.89×103 mg/kg in the spleen of a control mouse not administered with MNPs, whereas increases significantly to 1.93×103 mg/kg in that of a mouse administered with MNPs. Further, time kinetic analysis of biochemical and immunological parameters is required to confirm its suitability in bio-administration.
-
Choi Jong-Gu,Hasan Mahbub,Akter Hafeza,이상석 한국물리학회 2023 Current Applied Physics Vol.48 No.-
Conjugation of magnetic nanoparticles (MNPs) with biomolecules can be achieved through covalent or non-covalent bonding and chemisorption. By glutaraldehyde cross-linking, we successfully conjugated mouse monoclonal antibody (Ab) with amine-functionalized dextran-coated MNPs of diameter 50 nm. We aim to characterize the antibody-conjugated MNPs (Ab-MNPs) after buffer separation through low-gradient magnet and centrifugation. The magnetic separation/washing was achieved throughout the conjugation process by attaching the MNPs solution to a natural magnet for about 12 h. The centrifugation-based separation steps were done at 15,000×g for 30 min. The transmission electron microscope (TEM) data showed the flower-shaped core MNPs and their increased diameter after conjugation with Abs. The nanoparticle tracking analysis (NTA) also revealed the size distribution and drift velocities in MNPs and Ab-MNPs. The size distribution and concentration data showed an increased size and a lower value after conjugation, respectively. The drift velocities of Ab-MNPs prepared by the centrifugation method were significantly reduced than magnetic separation and standard MNPs.
-
-
Seong, Yunseo,Yoo, Young Sook,Akter, Hafeza,Kang, Min-Jung Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1060 No.-
<P><B>Abstract</B></P> <P>Proteomics is a valuable approach to discover biomarkers in human plasma for early diagnosis. However, detection of biomarkers in the plasma is still challenging because of its large protein content. In our study, we established albumin/IgG depletion methods for identification of low abundance proteins using two commercial kits with additional buffer conditions and various concentrations of cold acetone. Trypsin digestion, desalting, and data-dependent acquisition were also optimized. More than 80% depletion of albumin/IgG was achieved with two commercial kits and 98% depletion of albumin was obtained with 70% cold acetone. Recovery of four reference proteins, BNP (47–76), insulin, cytochrome c, and ubiquitin was obtained in all optimized methods. The best recovery of reference proteins was obtained using the ProteoExtract albumin/IgG removal kit with buffer A (61%–106%). After cold acetone precipitation, three reference proteins were recovered more than 48% except ubiquitin (12%). The number of identified proteins by Mascot was 28, 35, 17, and 34 for ProteoExtract, ProteoPrep, 70%, and 50% cold acetone, respectively. Furthermore, optimized methods detected MS/MS fragmentation patterns of elevated BNP in patient samples with cardiac disease. Our study provides the conditions for efficient biomarker discovery by minimal removing of high abundant proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Low abundance proteins as biomarker candidates were detected after simple sample preparation. </LI> <LI> UHPLC-MS/MS conditions and Mascot parameters were optimized for low abundance biomarker discovery. </LI> <LI> The established method was practically applied to discover BNP fragments from patient plasma samples with AMI. </LI> </UL> </P>
-
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
