<i>CYP2C19</i> haplotypes in Koreans as a marker of enzyme activity evaluated with omeprazole

Jin, S. K.,Kang, T. S.,Eom, S. O.,Kim, J.-I.,Lee, H. J.,Roh, J. Blackwell Publishing Ltd 2009 Journal of clinical pharmacy and therapeutics Vol.34 No.4

<P>Summary</P><P>Background and objective: </P><P>CYP2C19 is clinically important in Korea because of the relatively high incidence of poor metabolizers in the population. To fully understand the genetic mechanism of the <I>CYP2C19</I> defect in poor metabolizers, all variants need to be studied simultaneously. The aim of this study was to investigate the usefulness of <I>CYP2C19</I> haplotypes as a marker of CYP2C19 enzyme activity in Koreans.</P><P>Methods: </P><P>We analysed the single nucleotide polymorphisms and haplotypes of the <I>CYP2C19</I> gene in 150 healthy Koreans and found three major (frequency > 0·1) haplotypes (H1, H2 and H3). One oral dose of 40 mg omeprazole (Losec<SUP>®</SUP>) was administered to 30 subjects grouped as H1/H1, H2/H2, H1/H2, H1/H3 and H2/H3. The pharmacokinetics of omeprazole and its metabolites, 5-hydroxyomeprazole and omeprazole sulphone, in those groups was analysed.</P><P>Results and discussion: </P><P>The area under the plasma concentration–time curve (AUC<SUB>0→∞</SUB>) and elimination half-life (<I>T</I><SUB>1/2</SUB>) of omeprazole were significantly greater in the H2/H2 and H2/H3 groups than in the H1/H1 group (<I>P </I><<I> </I>0·05), whereas the metabolic ratios of omeprazole to 5-hydroxyomeprazole were also markedly higher.</P><P>Conclusion: </P><P>Although a specific SNP of <I>CYP2C19</I> may be predictive of enzyme activity, haplotyping is more reliable for identifying poor metabolizers in populations with variant alleles other than <I>CYP2C19*2</I> and <I>*3</I> alleles.</P>

Cryptotanshinone, a lipophilic compound of Salvia miltiorrriza root, inhibits TNF-α-induced expression of adhesion molecules in HUVEC and attenuates rat myocardial ischemia/reperfusion injury in vivo

Jin, Y.C.,Kim, C.W.,Kim, Y.M.,Nizamutdinova, I.T.,Ha, Y.M.,Kim, H.J.,Seo, H.G.,Son, K.H.,Jeon, S.J.,Kang, S.S.,Kim, Y.S.,Kam, S.C.,Lee, J.H.,Chang, K.C. North-Holland ; Elsevier Science Ltd 2009 european journal of pharmacology Vol.614 No.1

The aim of the present study was to evaluate the protective effect of cryptotanshinone (CTS), one of active ingredients of Salvia miltiorrhiza root, on myocardial ischemia-reperfusion injury in rat due to inhibition of some inflammatory events that occur by NF-kB-activation during ischemia and reperfusion. Myocardial ischemia and reperfusion injury was induced by occluding the left anterior descending coronary artery for 30 min followed by either 2 h (biochemical analysis) or 24 h (myocardial function and infarct size measurement) reperfusion. CTS injected (i.v.) 10 min before ischemia and reperfusion insult. CTS significantly reduced the infarct size and improved ischemia and reperfusion-induced myocardial contractile dysfunction. Furthermore, CTS inhibited NF-kB translocation, expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), neutrophil infiltration and MPO activity in ischemic myocardial tissues. CTS also significantly reduced plasma levels of TNF-α, IL-1β due to ischemia and reperfusion. Interestingly, H<SUB>2</SUB>O<SUB>2</SUB>-stimulated NF-kB-luciferase activity and TNF-α-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expressions in human umbilical vein endothelial cells (HUVEC) were significantly inhibited by CTS. Taken together, it is concluded that CTS may attenuate ischemia and reperfusion-induced microcirculatory disturbances by inhibition of proinflammatory cytokine production, reduction of neutrophil infiltration and possibly inhibition of adhesion molecules through inhibition of NF-kB-activation during ischemia and reperfusion.

Interface sulfur passivation using H₂S annealing for atomic-layer-deposited Al₂O₃ films on an ultrathin-body In_0.53Ga_0.47As-on-insulator

Jin, H.S.,Cho, Y.J.,Lee, S.M.,Kim, D.H.,Kim, D.W.,Lee, D.,Park, J.B.,Won, J.Y.,Lee, M.J.,Cho, S.H.,Hwang, C.S.,Park, T.J. New York] ; North-Holland 2014 APPLIED SURFACE SCIENCE - Vol.315 No.-

Atomic-layer-deposited Al<SUB>2</SUB>O<SUB>3</SUB> films were grown on ultrathin-body In<SUB>0.53</SUB>Ga<SUB>0.47</SUB>As substrates for III-V compound-semiconductor-based devices. Interface sulfur (S) passivation was performed with wet processing using ammonium sulfide ((NH<SUB>4</SUB>)<SUB>2</SUB>S) solution, and dry processing using post-deposition annealing (PDA) under a H<SUB>2</SUB>S atmosphere. The PDA under the H<SUB>2</SUB>S atmosphere resulted in a lower S concentration at the interface and a thicker interfacial layer than the case with (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment. The electrical properties of the device, including the interface property estimated through frequency dispersion in capacitance, were better for (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment than the PDA under a H<SUB>2</SUB>S atmosphere. They might be improved, however, by optimizing the process conditions of PDA. The PDA under a H<SUB>2</SUB>S atmosphere following (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment resulted in an increased S concentration at the interface, which improved the electrical properties of the devices.

UV Laser Rayleigh Scattering을 이용한 가스 성분의 농도 및 온도 분포 계측에 관한 실험적 연구

진성호(S.H.Jin),남기중(G.J.Nam),김희산(H.S.Kim),장래각(N.K.Chang),박승한(S.H.Park),깅웅(U.Kim),박경석(K.S.Park),김경수(G.S.Kim) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_2

Rayleigh Scattering Cross Sections(σ_i) of various gases and the temperature distributions of premixed Propane/O₂ flame are measured by high power KrF(248nm) Excimer laser and ICCD camera. Results show that σ_i of O₂ and Propane(C₃H_8) gases agree well in the 5% error range. but σ_i of H₂ has the more or less difference from the calculated value by other groups. This is attributed to the low RS signal of H₂ to noise level(SIN ratio).<br/> The temperature distributions of flame range out between 300K in the air and about 2000K in the burned area. In this temperature range, our system has the about 250K temperature resolution. Because low RS signals in the reaction area with high temperature are affected highly by noises, temperature uncertainty of this area is relatively high to another part of flame.<br/> Experimental results show that UV Rayleigh Scattering can be used for the measurement of mixing ratios of mixed gases and the temperature distributions of flame. Especially. this technique can be applied for the measurement of the mixing ratios of air/fuel before the ignition and the flame structure after the ignition inside the Engine.<br/>

Distinct clinical courses according to presenting phenotypes and their correlations to <i>ATP7B</i> mutations in a large Wilson's disease cohort

Lee, Beom H.,Kim, Joo H.,Lee, Sun Y.,Jin, Hye Y.,Kim, Kwi‐,Joo,Lee, Jin‐,Joo,Park, Jung‐,Young,Kim, Gu‐,Hwan,Choi, Jin‐,Ho,Kim, Kyung M.,Yoo, Han‐,Wook Blackwell Publishing Ltd 2011 Liver International Vol.31 No.6

<P><B>Abstract</B></P><P><B>Introduction and aims: </B> Wide phenotypic and genotypic heterogeneities in Wilson's disease (WD) have been reported, hampering the study of their correlations. The goal of this study was to identify the factors related to these diversities.</P><P><B>Methods: </B> Clinical courses and molecular genetic characteristics were analysed in 237 unrelated Korean WD families. The average follow‐up period was 8.2 ± 5.8 years.</P><P><B>Results: </B> Presenting phenotypes were classified as H1 (12.2%), H2 (42.4%), N1 (21.6%), N2 (0.4%), NX (0.4%), presymptomatic (22.4%) and other (0.4%), modifying the guidelines by Ferenci and colleagues. Age at presentation was youngest and cirrhosis was rarest in the presymptomatic group. Decompensated cirrhosis was the highest in the H1 group. Favourable outcome was rarest in the N1 group. Forty‐seven (11 novel) <I>ATP7B</I> mutations were identified in 85% of the 474 alleles. Multiplex ligation‐dependent probe amplification assays in <I>ATP7B</I> and analyses of <I>ATOX1</I> and <I>COMMD1</I> genes identified no additional mutations. Yeast complementation assays demonstrated functional perturbation of the seven novel missense mutants. Five major mutations, p.Arg778Leu, p.Ala874Val, p.Asn1270Ser, p.Lys838SerfsX35 and p.Leu1083Phe, accounted for 63% of the alleles. H1 was more common, age at presentation was younger and N1+N2+NX tended to be less common in patients with nonsense, frame shifting or splicing mutations than in those with missense mutations alone. Patients with both mutations in the transduction (Td) or the ATP hinge domain showed presymptomatic or hepatic manifestations but no neurological manifestation.</P><P><B>Conclusions: </B> The presenting phenotype strongly affects the clinical outcome of WD, and is related to the <I>ATP7B</I> mutation type and location, providing an evidence for genotype–phenotype correlations in WD.</P>

Effects of Fertilization Time and Culture Medium of Pig Oocytes Matured In Vitro by Liquid Boar Sperm Stored at 4℃

Park, C.S.,Yi, Y.J.,Kim, M.Y.,Chang, Y.J.,Lee, S.H.,Jin, D.I 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

This study was to investigate the effects of fertilization time and culture medium of pig oocytes matured in-vitro by liquid boar sperm. The sperm rich fraction (30∼60 ml) was slowly cooled to room temperature (20∼23℃) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min 800×g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of the LEN diluent to provide 1.0×10^(9) sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4℃. The medium used for oocyte maturation was TCM-199 supplemented with 26.19 mM sodium bicarbonate, 0.9 mM sodium pyruvate, 10μg/ml insulin, 2μg/ml vitamin B_(12), 25 mM HEPES, 10μg/ml bovine apotransferrin, 150μM cysteamine, 10IU/ml PMSG, 10 IU/ml hCG, 10 ng/ml EGF, 0.4% BSA, 75μg/ml sodium penicillin G, 50μg/ml streptomycin sulfate and 10% pFF. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5℃, 5% CO₂in air. Oocytes were inseminated with liquid boar sperm stored at 4℃ for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9 h in 500 μl mTBM fertilization media with 1.0×10^(6) sperm/ml concentration, respectively. Thereafter, oocytes were transferred into 500 μl NCSU-23, HEPES buffered NCSU-23, PZM-3 and PZM-4 culture media, respectively, for further culture of 6, 48 and 144 h. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times for 6 and 9 h than in those for 1 and 3 h. The rates of cleaved oocytes were higher in the fertilization times for 6 and 9 h (85.0 and 84.6%) than in those for 1 and 3 h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time for 6 h (33.6%) than in that for 1, 3 and 9 h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9, 27.6, 26.3 and 24.4 in the fertilization times for 6, 9, 3 and 1 h, respectively. The rate of blastocyst from the cleaved oocytes and the number of cells per blastocyst were higher in HEPES buffered NCSU-23 culture medium than in NCSU-23, PZM-3 and PZM-4 culture media. In conclusion, we found out that liquid boar sperm stored at 4℃ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend the coincubation time of 6 h in 500 μl TBM fertilization medium with 1×10^(6) sperm/ml concentration and the HEPES buffered NCSU-23 culture medium for in-vitro fertilization of pig oocytes matured in-vitro.

Enhancement of adhesion between polyphenylene sulfide and copper by surface treatments

Kim, J.Y.,Ahn, K.,Jeong, S.Y.,Jeong, E.D.,Jin, J.S.,Bae, J.S.,Kim, H.G.,Cho, C.R. Elsevier 2014 CURRENT APPLIED PHYSICS Vol.14 No.1

In this paper, we introduce methods which can effectively enhance the adhesion between polyphenylene sulfide (PPS) and bulk Cu. One of the methods involved the thermal evaporation of PPS to form a buffer layer on Cu and the other involved plasma treatment with reactive gases such as O<SUB>2</SUB>, H<SUB>2</SUB>, and N<SUB>2</SUB> on the PPS buffer layer. The adhesion strength of samples prepared by PPS thin film coating (~26 MPa) was largely enhanced when compared to that of samples obtained by only etching (~15 MPa). Among the samples obtained by plasma treatment using various reactive gases, the samples treated using H<SUB>2</SUB> plasma showed the best adhesion strength (of ~32 MPa) in comparison to the other samples owing to the adhesion between hydrophobic surfaces.

식품중의 수산분석법에 관한 연구

김복성,이영자,홍기형,이창희,류시생,진희숙,송성실,김용철,이명환 국립보건연구원 1992 국립보건원보 Vol.29 No.2

A study on biochemical and physiological characteristics of Lactobacillus pentosus LSDM isolated from Kimchi

Lim, S. D 韓國酪農學會 1996 韓國酪農學會誌 Vol.18 No.1

재래돼지(Korea Native Pigs) 특이 genetic marker와 육질 연관성 분석

김철욱,여정수,조광근,진상근,오명곤,박준규,권은정,홍연희,김지현,이보경,박다혜,김재우,이지홍 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6

본 연구는 재래돼지 특이적인 marker를 개발하고 이들 marker와 육질과의 연관성을 규명하기 위하여 실시하였다. 실험동물로 재래돼지와 랜드레이스 각각 30두씩 총 60두를 사용하였으며, 품종 특이 marker를 찾기 위하여 4번과 7번 염색체에 존재하는 총 60개의 microsatellite를 이용하여 PCR (polymerase chain reaction)을 수행하였다. PCR 증폭은 형광물질 Fam과 Hex, Ned로 표지된 한쌍의 microsatellite primer를 이용하였으며, 형광물질로 표식된 PCR 산물은 Genetic Analyzer ABI 310으로 전기영동하여 대립 유전자를 규명하고 통계 분석하였다. 대립 유전자에 대한 통계 분석 결과 총 60개의 microsatellite중 27개가 다양한 유전적 다형현상을 보이는 대립 유전자가 증폭되었으며 (p<0.05), 특히 SW1364와 SW445, SW1369, SWR773 microsatellite의 증폭 산물에서 재래돼지와 랜드레이스 두 품종간을 구분할 수 있는 품종 특이적인 대립 유전자를 확인하였다 (p<0.01). 또한 육질에 영향을 미치는 유전적 marker를 얻기 위해 60두 전체에 대한 육색, 지방색, 지방산 조성, 콜레스테롤 함량, 연도와 경도에 대한 육질 분석을 실시하여 육질 형질과 marker와의 연관성을 분석한 결과 육질 형질에 영향을 미치는 28개 marker를 확인하였으며, 육색과 지방색에 연관된 4개, 조직감과 연관된 10개의 marker를 확인하였다. 이렇게 선발된 marker들은 고급육 생산을 위한 돼지의 유전적 개량을 위하여 유용하게 이용될 수 있다. This study was conducted to develop Korean native pig-specific DNA markers and analyze its relativity with meat quality using 30 of Korean native pigs and Landraces, respectively. To find species-specific DNA markers, we selected and tested a total of 60 microsatellite markers on the 4 and 7 chromosome using polymerase chain reaction (PCR). PCR amplification was carried out by microsatellite primer pairs labeled with fluorescent Fam, Hex and Ned. The fluorescent labeled PCR products were electrophoresed with Genetic Analyzer ABI 310 followed by the allelic and statistical analysis. According to the allelic frequency annalysis, of the 60 microsatellite markers 27 were preduced with a variety of genetic polymorphic alleles (p<0.05). Especially, alleles of SW1364, SW445, SW1369 and SWR773 microsatellite markers were identified to be species-specific markers between two breeds (p<0.01). To analyze the meat quality we determined the meat color, fat color, fat composition, cholesterol contents and hardness, and compared these characteristics with the alleles of 36 species-specific markers in all pigs. Of the 60 microsatellite markers 28 were found to be related with meat quality; 18 for meat and fat color, 7 for fatty acid, 4 for cholesterol contents and 10 for shear force and hardness. These selected markers will be useful in the genetic improvement of pigs for an excellent meat quality.